(Circular) (Six-base) MAPSORT of: pF1KB6685.seq Check: 9961 from: 1 to: 4394 pF1KB6685 4394 bp With 182 enzymes: SgfI * October 7, 2009 14:22 .. AatII G_ACGT'C Cuts at: 722 722 Size: 4394 AccI GT'mk_AC Cuts at: 1373 1432 1373 Size: 59 4335 Acc65I G'GTAC_C Cuts at: 1410 1410 Size: 4394 AclI AA'CG_TT Cuts at: 4234 4234 Size: 4394 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 574 2219 2651 3398 3666 574 Size: 1645 432 747 268 1302 Fragments arranged by size: 1645 1302 747 432 268 AflIII A'CryG_T Cuts at: 519 1746 2850 3827 519 Size: 1227 1104 977 1086 Fragments arranged by size: 1227 1104 1086 977 AgeI A'CCGG_T Cuts at: 2782 2782 Size: 4394 AlwNI CAG_nnn'CTG Cuts at: 225 1699 3266 225 Size: 1474 1567 1353 Fragments arranged by size: 1567 1474 1353 ApaLI G'TGCA_C Cuts at: 3164 3164 Size: 4394 ApoI r'AATT_y Cuts at: 568 1032 1264 1398 3747 4003 568 Size: 464 232 134 2349 256 959 Fragments arranged by size: 2349 959 464 256 232 134 AseI AT'TA_AT Cuts at: 20 20 Size: 4394 AsiSI GCG_AT'CGC Cuts at: 104 104 Size: 4394 AvaI C'yCGr_G Cuts at: 318 677 1414 318 Size: 359 737 3298 Fragments arranged by size: 3298 737 359 BamHI G'GATC_C Cuts at: 1419 1419 Size: 4394 BanI G'GyrC_C Cuts at: 834 1228 1410 1824 2072 2107 834 Size: 394 182 414 248 35 3121 Fragments arranged by size: 3121 414 394 248 182 35 BanII G_rGCy'C Cuts at: 244 710 1408 2438 244 Size: 466 698 1030 2200 Fragments arranged by size: 2200 1030 698 466 BbeI G_GCGC'C Cuts at: 1828 2076 1828 Size: 248 4146 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 90 124 1353 1387 90 Size: 34 1229 34 3097 Fragments arranged by size: 3097 1229 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2292 3053 4394 2292 Size: 761 1341 2292 Fragments arranged by size: 2292 1341 761 BclI T'GATC_A Cuts at: 1914 1914 Size: 4394 BfrBI ATG'CAT Cuts at: 111 111 Size: 4394 BglII A'GATC_T Cuts at: 1909 3946 1909 Size: 2037 2357 BlpI GC'TnA_GC Cuts at: 346 1506 346 Size: 1160 3234 Bme1580I G_kGCm'C Cuts at: 640 839 2019 2112 3168 640 Size: 199 1180 93 1056 1866 Fragments arranged by size: 1866 1180 1056 199 93 BmgBI CAC'GTC Cuts at: 663 1215 663 Size: 552 3842 BmrI ACTGGGnnnn_n' Cuts at: 1606 1687 2022 4166 1606 Size: 81 335 2144 1834 Fragments arranged by size: 2144 1834 335 81 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2761 2761 Size: 4394 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 247 974 1198 247 Size: 727 224 3443 Fragments arranged by size: 3443 727 224 Bpu10I CC'TnA_GC Cuts at: 541 541 Size: 4394 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 238 433 694 768 1570 2449 2941 3239 238 Size: 195 261 74 802 879 492 298 1393 Fragments arranged by size: 1393 879 802 492 298 261 195 74 BsaI GGTCTCn'nnnn_ Cuts at: 307 4098 307 Size: 3791 603 BsaAI yAC'GTr Cuts at: 1747 1747 Size: 4394 BsaBI GATnn'nnATC Cuts at: 6 1908 3945 6 Size: 1902 2037 455 Fragments arranged by size: 2037 1902 455 BsaHI Gr'CG_yC Cuts at: 719 1825 2073 2775 4271 719 Size: 1106 248 702 1496 842 Fragments arranged by size: 1496 1106 842 702 248 BsaWI w'CCGG_w Cuts at: 1583 2104 2782 3056 3203 3614 1583 Size: 521 678 274 147 411 2363 Fragments arranged by size: 2363 678 521 411 274 147 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 121 121 Size: 4394 BseYI C'CCAG_C Cuts at: 247 1113 1819 2577 3154 3650 247 Size: 866 706 758 577 496 991 Fragments arranged by size: 991 866 758 706 577 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 157 466 157 Size: 309 4085 BsiEI CG_ry'CG Cuts at: 104 1936 1982 3190 104 Size: 1832 46 1208 1308 Fragments arranged by size: 1832 1308 1208 46 BsiHKAI G_wGCw'C Cuts at: 534 710 1408 2186 2376 3168 534 Size: 176 698 778 190 792 1760 Fragments arranged by size: 1760 792 778 698 190 176 BsmBI CGTCTCn'nnnn_ Cuts at: 917 917 Size: 4394 Bsp1286I G_dGCh'C Cuts at: 244 534 640 710 839 1408 2019 2112 2186 Size: 290 106 70 129 569 611 93 74 Cuts at: 2186 2376 2438 3168 244 Size: 190 62 730 1470 Fragments arranged by size: 1470 730 611 569 290 190 129 106 93 74 70 62 BspEI T'CCGG_A Cuts at: 3614 3614 Size: 4394 BspHI T'CATG_A Cuts at: 876 3570 876 Size: 2694 1700 BspMI ACCTGCnnnn'nnnn_ Cuts at: 134 1444 1658 1960 2341 134 Size: 1310 214 302 381 2187 Fragments arranged by size: 2187 1310 381 302 214 BsrBI CCG'CTC Cuts at: 50 2686 2740 4224 4389 50 Size: 2636 54 1484 165 55 Fragments arranged by size: 2636 1484 165 55 54 BsrDI GCAATG_nn' Cuts at: 2306 2306 Size: 4394 BsrFI r'CCGG_y Cuts at: 1307 2392 2573 2782 1307 Size: 1085 181 209 2919 Fragments arranged by size: 2919 1085 209 181 BssSI C'ACGA_G Cuts at: 2665 3023 2665 Size: 358 4036 BstAPI GCAn_nnn'nTGC Cuts at: 480 1771 480 Size: 1291 3103 BstBI TT'CG_AA Cuts at: 2755 2755 Size: 4394 BstYI r'GATC_y Cuts at: 1 407 1125 1419 1886 1909 2244 2490 3502 Size: 406 718 294 467 23 335 246 1012 Cuts at: 3502 3588 3600 3946 1 Size: 86 12 346 449 Fragments arranged by size: 1012 718 467 449 406 346 335 294 246 86 23 12 BtgI C'CryG_G Cuts at: 504 584 504 Size: 80 4314 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 2344 2525 2688 2344 Size: 181 163 4050 Fragments arranged by size: 4050 181 163 BtsI GCAGTG_nn' Cuts at: 534 1637 534 Size: 1103 3291 DraI TTT'AAA Cuts at: 1393 1393 Size: 4394 DrdI GACnn_nn'nnGTC Cuts at: 2100 2958 2100 Size: 858 3536 EaeI y'GGCC_r Cuts at: 165 501 587 1979 2153 2544 2571 3916 165 Size: 336 86 1392 174 391 27 1345 643 Fragments arranged by size: 1392 1345 643 391 336 174 86 27 EagI C'GGCC_G Cuts at: 1979 1979 Size: 4394 EarI CTCTTCn'nnn_ Cuts at: 613 971 1021 2417 2627 613 Size: 358 50 1396 210 2380 Fragments arranged by size: 2380 1396 358 210 50 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 2602 2912 3058 4204 2602 Size: 310 146 1146 2792 Fragments arranged by size: 2792 1146 310 146 EcoICRI GAG'CTC Cuts at: 708 1406 708 Size: 698 3696 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 247 574 974 1198 2219 2651 3398 3666 247 Size: 327 400 224 1021 432 747 268 975 Fragments arranged by size: 1021 975 747 432 400 327 268 224 EcoNI CCTnn'n_nnAGG Cuts at: 953 953 Size: 4394 EcoO109I rG'GnC_Cy Cuts at: 383 417 1533 383 Size: 34 1116 3244 Fragments arranged by size: 3244 1116 34 EcoRI G'AATT_C Cuts at: 1398 1398 Size: 4394 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 348 738 1224 2413 348 Size: 390 486 1189 2329 Fragments arranged by size: 2329 1189 486 390 FspI TGC'GCA Cuts at: 2175 2175 Size: 4394 HaeII r_GCGC'y Cuts at: 1828 2076 3098 4077 1828 Size: 248 1022 979 2145 Fragments arranged by size: 2145 1022 979 248 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1117 1149 2236 2266 2268 2298 1117 Size: 32 1087 30 2 30 3213 Fragments arranged by size: 3213 1087 32 30 30 2 HincII GTy'rAC Cuts at: 1374 1433 1374 Size: 59 4335 KasI G'GCGC_C Cuts at: 1824 2072 1824 Size: 248 4146 KpnI G_GTAC'C Cuts at: 1414 1414 Size: 4394 MluI A'CGCG_T Cuts at: 519 3827 519 Size: 3308 1086 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 123 3065 3249 123 Size: 2942 184 1268 Fragments arranged by size: 2942 1268 184 MscI TGG'CCA Cuts at: 167 2155 167 Size: 1988 2406 MslI CAynn'nnrTG Cuts at: 188 2383 2510 188 Size: 2195 127 2072 Fragments arranged by size: 2195 2072 127 MspA1I CmG'CkG Cuts at: 171 345 1505 1819 2179 3192 3437 171 Size: 174 1160 314 360 1013 245 1128 Fragments arranged by size: 1160 1128 1013 360 314 245 174 NaeI GCC'GGC Cuts at: 2575 2575 Size: 4394 NarI GG'CG_CC Cuts at: 1825 2073 1825 Size: 248 4146 NcoI C'CATG_G Cuts at: 504 584 504 Size: 80 4314 NgoMIV G'CCGG_C Cuts at: 2573 2573 Size: 4394 NruI TCG'CGA Cuts at: 1258 1258 Size: 4394 NsiI A_TGCA'T Cuts at: 113 113 Size: 4394 NspI r_CATG'y Cuts at: 1447 1947 2854 1447 Size: 500 907 2987 Fragments arranged by size: 2987 907 500 PasI CC'CwG_GG Cuts at: 378 378 Size: 4394 PciI A'CATG_T Cuts at: 2850 2850 Size: 4394 PflMI CCAn_nnn'nTGG Cuts at: 510 510 Size: 4394 PmeI GTTT'AAAC Cuts at: 1393 1393 Size: 4394 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3533 3565 3533 Size: 32 4362 PspXI vC'TCGA_Gb Cuts at: 677 677 Size: 4394 PstI C_TGCA'G Cuts at: 851 1242 1441 2126 851 Size: 391 199 685 3119 Fragments arranged by size: 3119 685 391 199 PvuI CG_AT'CG Cuts at: 104 104 Size: 4394 PvuII CAG'CTG Cuts at: 171 1819 2179 171 Size: 1648 360 2386 Fragments arranged by size: 2386 1648 360 RsrII CG'GwC_CG Cuts at: 2589 2589 Size: 4394 SacI G_AGCT'C Cuts at: 710 1408 710 Size: 698 3696 SalI G'TCGA_C Cuts at: 1372 1431 1372 Size: 59 4335 SapI GCTCTTCn'nnn_ Cuts at: 2417 2627 2417 Size: 210 4184 SbfI CC_TGCA'GG Cuts at: 1441 1441 Size: 4394 ScaI AGT'ACT Cuts at: 3911 3911 Size: 4394 SfcI C'TryA_G Cuts at: 33 847 1238 1437 2122 3115 3306 33 Size: 814 391 199 685 993 191 1121 Fragments arranged by size: 1121 993 814 685 391 199 191 SfoI GGC'GCC Cuts at: 1826 2074 1826 Size: 248 4146 SgfI GCG_AT'CGC Cuts at: 104 104 Size: 4394 SmaI CCC'GGG Cuts at: 320 1416 320 Size: 1096 3298 SmlI C'TyrA_G Cuts at: 253 412 677 709 783 1549 2464 2956 3218 Size: 159 265 32 74 766 915 492 262 Cuts at: 3218 253 Size: 1429 Fragments arranged by size: 1429 915 766 492 265 262 159 74 32 SpeI A'CTAG_T Cuts at: 67 67 Size: 4394 SphI G_CATG'C Cuts at: 1447 1947 1447 Size: 500 3894 StyI C'CwwG_G Cuts at: 504 584 954 1208 1528 1882 504 Size: 80 370 254 320 354 3016 Fragments arranged by size: 3016 370 354 320 254 80 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 1357 1977 2110 2569 2778 1357 Size: 620 133 459 209 2973 Fragments arranged by size: 2973 620 459 209 133 TatI w'GTAC_w Cuts at: 3909 3909 Size: 4394 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3911 3943 3911 Size: 32 4362 Tth111I GACn'n_nGTC Cuts at: 661 2191 661 Size: 1530 2864 XbaI T'CTAG_A Cuts at: 1425 1425 Size: 4394 XcmI CCAnnnn_n'nnnnTGG Cuts at: 375 1212 1889 375 Size: 837 677 2880 Fragments arranged by size: 2880 837 677 XhoI C'TCGA_G Cuts at: 677 677 Size: 4394 XmaI C'CCGG_G Cuts at: 318 1414 318 Size: 1096 3298 XmnI GAAnn'nnTTC Cuts at: 772 980 772 Size: 208 4186 ZraI GAC'GTC Cuts at: 720 720 Size: 4394 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BcgI BciVI BclI BfrBI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV NruI NsiI NspI PasI PciI PflMI PmeI PpiI PspXI PstI PvuI PvuII RsrII SacI SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AflII AhdI AleI AlfI AloI ApaI AscI AvrII BaeI BbsI BbvCI BglI BmtI BsaXI BsiWI BsmI BsrGI BssHII BstEII BstXI BstZ17I Bsu36I ClaI CspCI DraIII EcoRV FseI FspAI HindIII HpaI MfeI NdeI NheI NotI PacI PfoI PmlI PpuMI PshAI PsiI PspOMI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI SspI StuI SwaI