Restriction map for pF1KE1090
 (Linear) (Six-base) MAPSORT of: KIEE1090.seq  Check: 2706  from: 1  to: 1785



KIEE1090.orf

 With 182 enzymes: SgfI * 

                        October 13, 2009 15:05  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     861    1785 
   Size:        861     924
 
AflIII A'CryG_T

Cuts at:      0     851    1560    1785 
   Size:        851     709     225

  Fragments arranged by size: 

                 851     709     225
 
AleI CACnn'nnGTG

Cuts at:      0     190     850    1785 
   Size:        190     660     935

  Fragments arranged by size: 

                 935     660     190
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     369     401    1785 
   Size:        369      32    1384

  Fragments arranged by size: 

                1384     369      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0    1082    1715    1785 
   Size:       1082     633      70

  Fragments arranged by size: 

                1082     633      70
 
ApaLI G'TGCA_C

Cuts at:      0     181     250    1785 
   Size:        181      69    1535

  Fragments arranged by size: 

                1535     181      69
 
ApoI r'AATT_y

Cuts at:      0     437     979    1785 
   Size:        437     542     806

  Fragments arranged by size: 

                 806     542     437
 
AvaI C'yCGr_G

Cuts at:      0     358    1785 
   Size:        358    1427
 
BamHI G'GATC_C

Cuts at:      0      59     941    1727    1785 
   Size:         59     882     786      58

  Fragments arranged by size: 

                 882     786      59      58
 
BanI G'GyrC_C

Cuts at:      0      79     500     967    1427    1475    1785 
   Size:         79     421     467     460      48     310

  Fragments arranged by size: 

                 467     460     421     310      79      48
 
BanII G_rGCy'C

Cuts at:      0    1595    1785 
   Size:       1595     190
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     484     927    1785 
   Size:        484     443     858

  Fragments arranged by size: 

                 858     484     443
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     374    1785 
   Size:        374    1411
 
BclI T'GATC_A

Cuts at:      0     104    1785 
   Size:        104    1681
 
BglII A'GATC_T

Cuts at:      0     764    1785 
   Size:        764    1021
 
Bme1580I G_kGCm'C

Cuts at:      0     185     254     505    1785 
   Size:        185      69     251    1280

  Fragments arranged by size: 

                1280     251     185      69
 
BmgBI CAC'GTC

Cuts at:      0    1563    1785 
   Size:       1563     222
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     780    1785 
   Size:        780    1005
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1059    1501    1743    1785 
   Size:       1059     442     242      42

  Fragments arranged by size: 

                1059     442     242      42
 
BsaAI yAC'GTr

Cuts at:      0     102    1785 
   Size:        102    1683
 
BsaWI w'CCGG_w

Cuts at:      0     445     970    1730    1785 
   Size:        445     525     760      55

  Fragments arranged by size: 

                 760     525     445      55
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    1057    1087    1474    1504    1785 
   Size:       1057      30     387      30     281

  Fragments arranged by size: 

                1057     387     281      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     232    1384    1498    1785 
   Size:        232    1152     114     287

  Fragments arranged by size: 

                1152     287     232     114
 
BseYI C'CCAG_C

Cuts at:      0    1708    1785 
   Size:       1708      77
 
BsiHKAI G_wGCw'C

Cuts at:      0     185     254     572    1785 
   Size:        185      69     318    1213

  Fragments arranged by size: 

                1213     318     185      69
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0      34    1785 
   Size:         34    1751
 
Bsp1286I G_dGCh'C

Cuts at:      0     185     254     505     572    1595    1785 
   Size:        185      69     251      67    1023     190

  Fragments arranged by size: 

                1023     251     190     185      69      67
 
BspEI T'CCGG_A

Cuts at:      0    1730    1785 
   Size:       1730      55
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    1302    1785 
   Size:       1302     483
 
BsrFI r'CCGG_y

Cuts at:      0       3    1628    1785 
   Size:          3    1625     157

  Fragments arranged by size: 

                1625     157       3
 
BsrGI T'GTAC_A

Cuts at:      0    1004    1785 
   Size:       1004     781
 
BssSI C'ACGA_G

Cuts at:      0     612    1785 
   Size:        612    1173
 
BstYI r'GATC_y

Cuts at:      0      59     764     941    1405    1727    1785 
   Size:         59     705     177     464     322      58

  Fragments arranged by size: 

                 705     464     322     177      59      58
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0    1312    1785 
   Size:       1312     473
 
BtsI GCAGTG_nn'

Cuts at:      0      27     521     801    1785 
   Size:         27     494     280     984

  Fragments arranged by size: 

                 984     494     280      27
 
EaeI y'GGCC_r

Cuts at:      0     597    1037    1785 
   Size:        597     440     748

  Fragments arranged by size: 

                 748     597     440
 
EarI CTCTTCn'nnn_

Cuts at:      0     515    1741    1785 
   Size:        515    1226      44

  Fragments arranged by size: 

                1226     515      44
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     761    1785 
   Size:        761    1024
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     861    1059    1501    1743    1785 
   Size:        861     198     442     242      42

  Fragments arranged by size: 

                 861     442     242     198      42
 
EcoO109I rG'GnC_Cy

Cuts at:      0     525    1511    1785 
   Size:        525     986     274

  Fragments arranged by size: 

                 986     525     274
 
EcoRI G'AATT_C

Cuts at:      0     437    1785 
   Size:        437    1348
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     111    1785 
   Size:        111    1674
 
HaeII r_GCGC'y

Cuts at:      0    1606    1671    1785 
   Size:       1606      65     114

  Fragments arranged by size: 

                1606     114      65
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     651     683    1057    1089    1785 
   Size:        651      32     374      32     696

  Fragments arranged by size: 

                 696     651     374      32      32
 
HincII GTy'rAC

Cuts at:      0    1137    1785 
   Size:       1137     648
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     711    1785 
   Size:        711    1074
 
MslI CAynn'nnrTG

Cuts at:      0     190     850    1228    1785 
   Size:        190     660     378     557

  Fragments arranged by size: 

                 660     557     378     190
 
MspA1I CmG'CkG

Cuts at:      0      17     156     348     996    1042    1380    1712    1721 
   Size:         17     139     192     648      46     338     332       9

Cuts at:   1721    1785 
   Size:         64

  Fragments arranged by size: 

                 648     338     332     192     139      64      46      17
                   9
 
NaeI GCC'GGC

Cuts at:      0       5    1630    1785 
   Size:          5    1625     155

  Fragments arranged by size: 

                1625     155       5
 
NgoMIV G'CCGG_C

Cuts at:      0       3    1628    1785 
   Size:          3    1625     157

  Fragments arranged by size: 

                1625     157       3
 
PflMI CCAn_nnn'nTGG

Cuts at:      0      89    1686    1785 
   Size:         89    1597      99

  Fragments arranged by size: 

                1597      99      89
 
PfoI T'CCnGG_A

Cuts at:      0    1356    1785 
   Size:       1356     429
 
PmlI CAC'GTG

Cuts at:      0     102    1785 
   Size:        102    1683
 
PstI C_TGCA'G

Cuts at:      0      16      22    1571    1785 
   Size:         16       6    1549     214

  Fragments arranged by size: 

                1549     214      16       6
 
PvuII CAG'CTG

Cuts at:      0      17     996    1380    1712    1785 
   Size:         17     979     384     332      73

  Fragments arranged by size: 

                 979     384     332      73      17
 
ScaI AGT'ACT

Cuts at:      0     892    1785 
   Size:        892     893
 
SfcI C'TryA_G

Cuts at:      0      12      18    1149    1567    1647    1785 
   Size:         12       6    1131     418      80     138

  Fragments arranged by size: 

                1131     418     138      80      12       6
 
StyI C'CwwG_G

Cuts at:      0     188    1785 
   Size:        188    1597
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     410    1021    1785 
   Size:        410     611     764

  Fragments arranged by size: 

                 764     611     410
 
TatI w'GTAC_w

Cuts at:      0     890    1004    1760    1785 
   Size:        890     114     756      25

  Fragments arranged by size: 

                 890     756     114      25
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1474    1506    1785 
   Size:       1474      32     279

  Fragments arranged by size: 

                1474     279      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     513    1326    1785 
   Size:        513     813     459

  Fragments arranged by size: 

                 813     513     459
 
XmnI GAAnn'nnTTC

Cuts at:      0     301    1785 
   Size:        301    1484
 

 Enzymes that do cut:

     AcuI   AflIII     AleI     AloI    AlwNI    ApaLI     ApoI     AvaI
    BamHI     BanI    BanII     BbsI    BciVI     BclI    BglII Bme1580I
    BmgBI     BmrI     BpmI    BsaAI    BsaWI    BsaXI    BseRI    BseYI
  BsiHKAI    BsmBI Bsp1286I    BspEI    BspMI    BsrFI    BsrGI    BssSI
    BstYI    BtgZI     BtsI     EaeI     EarI     EciI  Eco57MI EcoO109I
    EcoRI     FalI    HaeII    Hin4I   HincII     MmeI     MslI   MspA1I
     NaeI   NgoMIV    PflMI     PfoI     PmlI     PstI    PvuII     ScaI
     SfcI     StyI    TaqII     TatI     TstI     XcmI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII     AgeI
     AhdI     AlfI     ApaI     AscI     AseI    AsiSI    AvrII     BaeI
     BbeI    BbvCI     BcgI    BfrBI     BglI     BlpI     BmtI     BplI
   Bpu10I    BpuEI     BsaI    BsaBI    BsaHI     BsgI    BsiEI    BsiWI
     BsmI    BspHI    BsrBI    BsrDI   BssHII   BstAPI    BstBI   BstEII
    BstXI  BstZ17I   Bsu36I     BtgI     ClaI    CspCI     DraI   DraIII
     DrdI     EagI  EcoICRI    EcoNI    EcoRV     FseI     FspI    FspAI
  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MscI     NarI
     NcoI     NdeI     NheI     NotI     NruI     NsiI     NspI     PacI
     PasI     PciI     PmeI     PpiI    PpuMI    PshAI     PsiI   PspOMI
    PspXI     PsrI     PvuI    RsrII     SacI    SacII     SalI    SanDI
     SapI     SbfI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI
     SmlI    SnaBI     SpeI     SphI     SrfI     SspI     StuI     SwaI
  Tth111I     XbaI     XhoI     XmaI     ZraI
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com