Restriction map for pF1KB5715
##################################
# NO CUT ENZYMES (   95 enzymes) #
##################################

AatII       Acc65I      AclI        AcsI        AflII       
AgeI        AhdI        Aor51HI     ApoI        AscI        
AseI        AsnI        Asp700I     Asp718I     AspEI       
AviII       AvrII       BbrPI       BbsI        BciVI       
BclI        BfaI        BfrI        BlnI        BpuAI       
BsaAI       BsaBI       BsiWI       BsmBI       Bsp1407I    
BspDI       BspLU11I    BsrDI       BsrGI       BssHII      
BstBI       BstEII      BstPI       ClaI        CpoI        
DraI        DrdI        Eam1105I    Eco47III    EcoO65I     
EcoRI       EcoRV       EcoT22I     FbaI        FseI        
FspI        HindIII     HpaI        KpnI        KspI        
MaeI        MamI        MfeI        MluI        MseI        
MunI        NaeI        NcoI        NdeI        NgoMIV      
NheI        NotI        NruI        NsiI        NspV        
PacI        PinAI       PmaCI       PmeI        PmlI        
PshBI       Psp1406I    PvuI        RsrII       SacII       
SalI        SbfI        ScaI        SfuI        SgrAI       
SnaBI       SpeI        Sse8387I    SspBI       SspI        
SwaI        Tru9I       Tsp509I     XbaI        XmnI        


###################################
# ONE CUT ENZYMES (   50 enzymes) #
###################################

AccI          385  GTATAC               GT^[AC][GT]AC
AccIII        335  TCCGGA               T^CCGGA
AcyI         2055  GGCGCC               G[GA]^CG[CT]C
AflIII        227  ACGTGT               A^C[GA][CT]GT
Alw44I       2252  GTGCAC               G^TGCAC
AocI         2230  CCTCAGG              CC^T[ACGT]AGG
ApaLI        2252  GTGCAC               G^TGCAC
AspI          930  GACTCTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BalI          746  TGGCCA               TGG^CCA
BamHI        2235  GGATCC               G^GATCC
BbeI         2055  GGCGCC               GGCGC^C
BcgI          536  CGAATGAGGTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglII        1979  AGATCT               A^GATCT
BsaHI        2055  GGCGCC               G[GA]^CG[CT]C
BsaWI         335  TCCGGA               [AT]^CCGG[AT]
BseAI         335  TCCGGA               T^CCGGA
BsmI          339  GAATGC               GAATGC(1/-1)
BspEI         335  TCCGGA               T^CCGGA
BspHI        1268  TCATGA               T^CATGA
BsrFI        2256  ACCGGC               [GA]^CCGG[CT]
Bst1107I      385  GTATAC               GTA^TAC
BstZ17I       385  GTATAC               GTA^TAC
Bsu36I       2230  CCTCAGG              CC^T[ACGT]AGG
Cfr10I       2256  ACCGGC               [GA]^CCGG[CT]
DraIII       1774  CACACTGTG            CAC[ACGT][ACGT][ACGT]^GTG
EagI          192  CGGCCG               C^GGCCG
EclXI         192  CGGCCG               C^GGCCG
Eco52I        192  CGGCCG               C^GGCCG
Eco81I       2230  CCTCAGG              CC^T[ACGT]AGG
HgaI          941  GCGTC                GACGC(5/10)
Hin1I        2055  GGCGCC               G[GA]^CG[CT]C
HincII       1653  GTTGAC               GT[CT]^[GA]AC
HindII       1653  GTTGAC               GT[CT]^[GA]AC
KasI         2055  GGCGCC               G^GCGCC
MluNI         746  TGGCCA               TGG^CCA
MroI          335  TCCGGA               T^CCGGA
MscI          746  TGGCCA               TGG^CCA
NarI         2055  GGCGCC               GG^CGCC
PaeR7I         93  CTCGAG               C^TCGAG
PflFI         930  GACTCTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI          71  GGGACCC              [GA]G^G[AT]CC[CT]
PshAI        2190  GACCCTGGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI         1268  TCATGA               T^CATGA
SfiI          705  GGCCTTCGGGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI         2055  GGCGCC               GGC^GCC
SmlI           93  CTCGAG               C^T[CT][GA]AG
SphI         1769  GCATGC               GCATG^C
StuI         1712  AGGCCT               AGG^CCT
Tth111I       930  GACTCTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XhoI           93  CTCGAG               C^TCGAG


###################################
# TWO CUT ENZYMES (   22 enzymes) #
###################################

ApaI         1792  GGGCCC               GGGCC^C
             1853  GGGCCC              
BbvCI        1829  CCTCAGC              CCTCAGC(-5/-2)
             2113  CCTCAGC             
BlpI          180  GCTGAGC              GC^T[ACGT]AGC
              673  GCTGAGC             
BpmI         1931  CTCCAG               CTGGAG(16/14)
             1632  CTGGAG              
Bpu1102I      180  GCTGAGC              GC^T[ACGT]AGC
              673  GCTGAGC             
BsaI         1339  GAGACC               GGTCTC(1/5)
             2196  GGTCTC              
BsiEI         107  CGACCG               CG[GA][CT]^CG
              192  CGGCCG              
BssSI        1729  CACGAG               CACGAG(-5/-1)
              316  CTCGTG              
BstAPI        641  GCATCCAGTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
             1120  GCAATCCTTGC         
BstYI        1979  AGATCT               [GA]^GATC[CT]
             2235  GGATCC              
CelII         180  GCTGAGC              GC^T[ACGT]AGC
              673  GCTGAGC             
DsaI         1796  CCGTGG               C^C[GA][CT]GG
             2264  CCGTGG              
EcoNI         435  CCTCTGCCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
             1643  CCTGGTGCAGG         
HaeII        2000  AGCGCC               [GA]GCGC^[CT]
             2055  GGCGCC              
MflI         1979  AGATCT               [GA]^GATC[CT]
             2235  GGATCC              
SacI           31  GAGCTC               GAGCT^C
             1732  GAGCTC              
SexAI        1090  ACCTGGT              A^CC[AT]GGT
             1642  ACCTGGT             
SmaI           75  CCCGGG               CCC^GGG
             1300  CCCGGG              
TfiI          777  GAATC                G^A[AT]TC
             1530  GAATC               
XhoII        1979  AGATCT               [GA]^GATC[CT]
             2235  GGATCC              
XmaCI          75  CCCGGG               C^CCGGG
             1300  CCCGGG              
XmaI           75  CCCGGG               C^CCGGG
             1300  CCCGGG              


#########################################
# 3 OR MORE CUT ENZYMES (   91 enzymes) #
#########################################

AccII       AciI        AfaI        AluI        AlwI        
AlwNI       AspHI       AvaI        AvaII       BanI        
BanII       BbvI        BcnI        BglI        BmyI        
BsaJI       BseRI       BsgI        BsiHKAI     BsiYI       
BslI        BsmAI       BsmFI       BsoBI       Bsp1286I    
BspMI       BsrBI       BsrI        BssKI       BstF5I      
BstNI       BstUI       BstXI       Cac8I       CfoI        
Cfr13I      DdeI        DpnII       DraII       EaeI        
EarI        EcoO109I    EcoRII      EcoT14I     FauI        
Fnu4HI      FokI        HaeIII      HapII       HhaI        
HinP1I      HinfI       HpaII       HphI        ItaI        
Ksp632I     MaeII       MaeIII      MboI        MboII       
MnlI        MslI        MspA1I      MspI        MvaI        
MvnI        MwoI        NciI        NdeII       NlaIII      
NlaIV       NspI        PflMI       PleI        PstI        
PvuII       RsaI        SapI        Sau3AI      Sau96I      
ScrFI       SfaNI       SfcI        StyI        TaqI        
TseI        Tsp45I      TspRI       TthHB8I     Van91I      
XcmI        
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