Restriction map for pF1KB5715
 (Linear) (Six-base) MAPSORT of: KIBB5715.seq  Check: 3374  from: 1  to: 2361



KIBB5715.orf

 With 182 enzymes: SgfI * 

                        March 2, 2010 14:26  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    1091    1128    2137    2361 
   Size:       1091      37    1009     224

  Fragments arranged by size: 

                1091    1009     224      37
 
AccI GT'mk_AC

Cuts at:      0     386    2361 
   Size:        386    1975
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     545    1383    1749    2361 
   Size:        545     838     366     612

  Fragments arranged by size: 

                 838     612     545     366
 
AflIII A'CryG_T

Cuts at:      0     227    2361 
   Size:        227    2134
 
AleI CACnn'nnGTG

Cuts at:      0     754    1921    2361 
   Size:        754    1167     440

  Fragments arranged by size: 

                1167     754     440
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     334    2361 
   Size:        334    2027
 
AlwNI CAG_nnn'CTG

Cuts at:      0     372     673    1758    2295    2361 
   Size:        372     301    1085     537      66

  Fragments arranged by size: 

                1085     537     372     301      66
 
ApaI G_GGCC'C

Cuts at:      0    1796    1857    2361 
   Size:       1796      61     504

  Fragments arranged by size: 

                1796     504      61
 
ApaLI G'TGCA_C

Cuts at:      0    2252    2361 
   Size:       2252     109
 
AvaI C'yCGr_G

Cuts at:      0      75      93     449    1059    1300    1536    2361 
   Size:         75      18     356     610     241     236     825

  Fragments arranged by size: 

                 825     610     356     241     236      75      18
 
BamHI G'GATC_C

Cuts at:      0    2235    2361 
   Size:       2235     126
 
BanI G'GyrC_C

Cuts at:      0     862     895    1039    2055    2149    2361 
   Size:        862      33     144    1016      94     212

  Fragments arranged by size: 

                1016     862     212     144      94      33
 
BanII G_rGCy'C

Cuts at:      0      35     241     917    1736    1796    1829    1857    2222 
   Size:         35     206     676     819      60      33      28     365

Cuts at:   2222    2277    2361 
   Size:         55      84

  Fragments arranged by size: 

                 819     676     365     206      84      60      55      35
                  33      28
 
BbeI G_GCGC'C

Cuts at:      0    2059    2361 
   Size:       2059     302
 
BbvCI CC'TCA_GC

Cuts at:      0    1830    2114    2361 
   Size:       1830     284     247

  Fragments arranged by size: 

                1830     284     247
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     525     559    2361 
   Size:        525      34    1802

  Fragments arranged by size: 

                1802     525      34
 
BglI GCCn_nnn'nGGC

Cuts at:      0     191     712     976    2266    2291    2361 
   Size:        191     521     264    1290      25      70

  Fragments arranged by size: 

                1290     521     264     191      70      25
 
BglII A'GATC_T

Cuts at:      0    1979    2361 
   Size:       1979     382
 
BlpI GC'TnA_GC

Cuts at:      0     181     674    2361 
   Size:        181     493    1687

  Fragments arranged by size: 

                1687     493     181
 
Bme1580I G_kGCm'C

Cuts at:      0     587     867    1042    1796    1857    1940    2050    2256 
   Size:        587     280     175     754      61      83     110     206

Cuts at:   2256    2361 
   Size:        105

  Fragments arranged by size: 

                 754     587     280     206     175     110     105      83
                  61
 
BmgBI CAC'GTC

Cuts at:      0     627    2361 
   Size:        627    1734
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     843    1667    2087    2216    2243    2361 
   Size:        843     824     420     129      27     118

  Fragments arranged by size: 

                 843     824     420     129     118      27
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     936    2361 
   Size:        936    1425
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1653    1916    2361 
   Size:       1653     263     445

  Fragments arranged by size: 

                1653     445     263
 
Bpu10I CC'TnA_GC

Cuts at:      0    1830    2066    2114    2361 
   Size:       1830     236      48     247

  Fragments arranged by size: 

                1830     247     236      48
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    1333    2202    2361 
   Size:       1333     869     159

  Fragments arranged by size: 

                1333     869     159
 
BsaHI Gr'CG_yC

Cuts at:      0    2056    2361 
   Size:       2056     305
 
BsaWI w'CCGG_w

Cuts at:      0     335    2361 
   Size:        335    2026
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     153     183     274     304    2361 
   Size:        153      30      91      30    2057

  Fragments arranged by size: 

                2057     153      91      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     159     162    1277    1833    1901    2037    2361 
   Size:        159       3    1115     556      68     136     324

  Fragments arranged by size: 

                1115     556     324     159     136      68       3
 
BseYI C'CCAG_C

Cuts at:      0      15      66     353     424    1856    2161    2361 
   Size:         15      51     287      71    1432     305     200

  Fragments arranged by size: 

                1432     305     287     200      71      51      15
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     489     516     565     714     908    1668    2361 
   Size:        489      27      49     149     194     760     693

  Fragments arranged by size: 

                 760     693     489     194     149      49      27
 
BsiEI CG_ry'CG

Cuts at:      0     110     195    2361 
   Size:        110      85    2166

  Fragments arranged by size: 

                2166     110      85
 
BsiHKAI G_wGCw'C

Cuts at:      0      35     323     680    1235    1391    1600    1736    2256 
   Size:         35     288     357     555     156     209     136     520

Cuts at:   2256    2361 
   Size:        105

  Fragments arranged by size: 

                 555     520     357     288     209     156     136     105
                  35
 
BsmI GAATG_Cn'

Cuts at:      0     345    2361 
   Size:        345    2016
 
Bsp1286I G_dGCh'C

Cuts at:      0      35     241     323     587     680     867     917    1042 
   Size:         35     206      82     264      93     187      50     125

Cuts at:   1042    1235    1391    1600    1736    1796    1829    1857    1940 
   Size:        193     156     209     136      60      33      28      83

Cuts at:   1940    2050    2222    2256    2277    2361 
   Size:        110     172      34      21      84

  Fragments arranged by size: 

                 264     209     206     193     187     172     156     136
                 125     110      93      84      83      82      60      50
                  35      34      33      28      21
 
BspEI T'CCGG_A

Cuts at:      0     335    2361 
   Size:        335    2026
 
BspHI T'CATG_A

Cuts at:      0    1268    2361 
   Size:       1268    1093
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    1091    1128    1640    2137    2361 
   Size:       1091      37     512     497     224

  Fragments arranged by size: 

                1091     512     497     224      37
 
BsrBI CCG'CTC

Cuts at:      0      39      98     480     660    2361 
   Size:         39      59     382     180    1701

  Fragments arranged by size: 

                1701     382     180      59      39
 
BsrFI r'CCGG_y

Cuts at:      0    2256    2361 
   Size:       2256     105
 
BssSI C'ACGA_G

Cuts at:      0     316    1729    2361 
   Size:        316    1413     632

  Fragments arranged by size: 

                1413     632     316
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     647    1126    2361 
   Size:        647     479    1235

  Fragments arranged by size: 

                1235     647     479
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     273     756    1875    2325    2361 
   Size:        273     483    1119     450      36

  Fragments arranged by size: 

                1119     483     450     273      36
 
BstYI r'GATC_y

Cuts at:      0    1979    2235    2361 
   Size:       1979     256     126

  Fragments arranged by size: 

                1979     256     126
 
BstZ17I GTA'TAC

Cuts at:      0     387    2361 
   Size:        387    1974
 
Bsu36I CC'TnA_GG

Cuts at:      0    2231    2361 
   Size:       2231     130
 
BtgI C'CryG_G

Cuts at:      0    1796    2264    2361 
   Size:       1796     468      97

  Fragments arranged by size: 

                1796     468      97
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     684    1572    2361 
   Size:        684     888     789

  Fragments arranged by size: 

                 888     789     684
 
BtsI GCAGTG_nn'

Cuts at:      0     186     268     365    2361 
   Size:        186      82      97    1996

  Fragments arranged by size: 

                1996     186      97      82
 
DraIII CAC_nnn'GTG

Cuts at:      0    1779    2361 
   Size:       1779     582
 
EaeI y'GGCC_r

Cuts at:      0       2     192     275     746    2280    2361 
   Size:          2     190      83     471    1534      81

  Fragments arranged by size: 

                1534     471     190      83      81       2
 
EagI C'GGCC_G

Cuts at:      0     192    2361 
   Size:        192    2169
 
EarI CTCTTCn'nnn_

Cuts at:      0     667     742     805    1687    2014    2023    2361 
   Size:        667      75      63     882     327       9     338

  Fragments arranged by size: 

                 882     667     338     327      75      63       9
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     161     783    2361 
   Size:        161     622    1578

  Fragments arranged by size: 

                1578     622     161
 
EcoICRI GAG'CTC

Cuts at:      0      33    1734    2361 
   Size:         33    1701     627

  Fragments arranged by size: 

                1701     627      33
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     545    1383    1653    1749    1916    2361 
   Size:        545     838     270      96     167     445

  Fragments arranged by size: 

                 838     545     445     270     167      96
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     439    1647    2361 
   Size:        439    1208     714

  Fragments arranged by size: 

                1208     714     439
 
EcoO109I rG'GnC_Cy

Cuts at:      0      72     454    2292    2299    2361 
   Size:         72     382    1838       7      62

  Fragments arranged by size: 

                1838     382      72      62       7
 
HaeII r_GCGC'y

Cuts at:      0    2004    2059    2361 
   Size:       2004      55     302

  Fragments arranged by size: 

                2004     302      55
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     153     185     274     306     863     895     919     951 
   Size:        153      32      89      32     557      32      24      32

Cuts at:    951    1135    1167    1515    1547    1828    1860    1863    1895 
   Size:        184      32     348      32     281      32       3      32

Cuts at:   1895    2335    2361 
   Size:        440      26

  Fragments arranged by size: 

                 557     440     348     281     184     153      89      32
                  32      32      32      32      32      32      32      26
                  24       3
 
HincII GTy'rAC

Cuts at:      0    1655    2361 
   Size:       1655     706
 
KasI G'GCGC_C

Cuts at:      0    2055    2361 
   Size:       2055     306
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    2225    2361 
   Size:       2225     136
 
MscI TGG'CCA

Cuts at:      0     748    2361 
   Size:        748    1613
 
MslI CAynn'nnrTG

Cuts at:      0     754    1591    1870    1921    2361 
   Size:        754     837     279      51     440

  Fragments arranged by size: 

                 837     754     440     279      51
 
MspA1I CmG'CkG

Cuts at:      0       7     180     216     350    1403    1834    2052    2118 
   Size:          7     173      36     134    1053     431     218      66

Cuts at:   2118    2361 
   Size:        243

  Fragments arranged by size: 

                1053     431     243     218     173     134      66      36
                   7
 
NarI GG'CG_CC

Cuts at:      0    2056    2361 
   Size:       2056     305
 
NspI r_CATG'y

Cuts at:      0     393     690    1773    1864    2361 
   Size:        393     297    1083      91     497

  Fragments arranged by size: 

                1083     497     393     297      91
 
PasI CC'CwG_GG

Cuts at:      0    1035    2361 
   Size:       1035    1326
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     614     855    1961    2361 
   Size:        614     241    1106     400

  Fragments arranged by size: 

                1106     614     400     241
 
PfoI T'CCnGG_A

Cuts at:      0    1451    2361 
   Size:       1451     910
 
PpuMI rG'GwC_Cy

Cuts at:      0      72    2361 
   Size:         72    2289
 
PshAI GACnn'nnGTC

Cuts at:      0    2194    2361 
   Size:       2194     167
 
PspOMI G'GGCC_C

Cuts at:      0    1792    1853    2361 
   Size:       1792      61     508

  Fragments arranged by size: 

                1792     508      61
 
PspXI vC'TCGA_Gb

Cuts at:      0      93    2361 
   Size:         93    2268
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0    1177    1209    2361 
   Size:       1177      32    1152

  Fragments arranged by size: 

                1177    1152      32
 
PstI C_TGCA'G

Cuts at:      0    1742    1997    2148    2361 
   Size:       1742     255     151     213

  Fragments arranged by size: 

                1742     255     213     151
 
PvuII CAG'CTG

Cuts at:      0    1403    1834    2118    2361 
   Size:       1403     431     284     243

  Fragments arranged by size: 

                1403     431     284     243
 
SacI G_AGCT'C

Cuts at:      0      35    1736    2361 
   Size:         35    1701     625

  Fragments arranged by size: 

                1701     625      35
 
SanDI GG'GwC_CC

Cuts at:      0      72    2361 
   Size:         72    2289
 
SapI GCTCTTCn'nnn_

Cuts at:      0     667     742    1687    2361 
   Size:        667      75     945     674

  Fragments arranged by size: 

                 945     674     667      75
 
SexAI A'CCwGG_T

Cuts at:      0    1090    1642    2361 
   Size:       1090     552     719

  Fragments arranged by size: 

                1090     719     552
 
SfcI C'TryA_G

Cuts at:      0    1738    1993    2144    2361 
   Size:       1738     255     151     217

  Fragments arranged by size: 

                1738     255     217     151
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0     712    2361 
   Size:        712    1649
 
SfoI GGC'GCC

Cuts at:      0    2057    2361 
   Size:       2057     304
 
SmaI CCC'GGG

Cuts at:      0      77    1302    2361 
   Size:         77    1225    1059

  Fragments arranged by size: 

                1225    1059      77
 
SmlI C'TyrA_G

Cuts at:      0      93    2361 
   Size:         93    2268
 
SphI G_CATG'C

Cuts at:      0    1773    2361 
   Size:       1773     588
 
StuI AGG'CCT

Cuts at:      0    1714    2361 
   Size:       1714     647
 
StyI C'CwwG_G

Cuts at:      0     587    1613    1709    2361 
   Size:        587    1026      96     652

  Fragments arranged by size: 

                1026     652     587      96
 
TatI w'GTAC_w

Cuts at:      0     962    2361 
   Size:        962    1399
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1765    1797    2361 
   Size:       1765      32     564

  Fragments arranged by size: 

                1765     564      32
 
Tth111I GACn'n_nGTC

Cuts at:      0     933    2361 
   Size:        933    1428
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     856    1350    1962    2361 
   Size:        856     494     612     399

  Fragments arranged by size: 

                 856     612     494     399
 
XhoI C'TCGA_G

Cuts at:      0      93    2361 
   Size:         93    2268
 
XmaI C'CCGG_G

Cuts at:      0      75    1300    2361 
   Size:         75    1225    1061

  Fragments arranged by size: 

                1225    1061      75
 

 Enzymes that do cut:

     AarI     AccI     AcuI   AflIII     AleI     AlfI    AlwNI     ApaI
    ApaLI     AvaI    BamHI     BanI    BanII     BbeI    BbvCI     BcgI
     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI     BplI     BpmI
   Bpu10I     BsaI    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI
    BsiEI  BsiHKAI     BsmI Bsp1286I    BspEI    BspHI    BspMI    BsrBI
    BsrFI    BssSI   BstAPI    BstXI    BstYI  BstZ17I   Bsu36I     BtgI
    BtgZI     BtsI   DraIII     EaeI     EagI     EarI     EciI  EcoICRI
  Eco57MI    EcoNI EcoO109I    HaeII    Hin4I   HincII     KasI     MmeI
     MscI     MslI   MspA1I     NarI     NspI     PasI    PflMI     PfoI
    PpuMI    PshAI   PspOMI    PspXI     PsrI     PstI    PvuII     SacI
    SanDI     SapI    SexAI     SfcI     SfiI     SfoI     SmaI     SmlI
     SphI     StuI     StyI     TatI     TstI  Tth111I     XcmI     XhoI
     XmaI

 Enzymes that do not cut: 

    AatII   Acc65I     AclI     AfeI    AflII     AgeI     AhdI     AloI
     ApoI     AscI     AseI    AsiSI    AvrII     BaeI     BbsI    BciVI
     BclI    BfrBI     BmtI    BpuEI    BsaAI    BsaBI    BsiWI    BsmBI
    BsrDI    BsrGI   BssHII    BstBI   BstEII     ClaI    CspCI     DraI
     DrdI    EcoRI    EcoRV     FalI     FseI     FspI    FspAI  HindIII
     HpaI     KpnI     MfeI     MluI     NaeI     NcoI     NdeI   NgoMIV
     NheI     NotI     NruI     NsiI     PacI     PciI     PmeI     PmlI
     PpiI     PsiI     PvuI    RsrII    SacII     SalI     SbfI     ScaI
     SgfI    SgrAI    SnaBI     SpeI     SrfI     SspI     SwaI    TaqII
     XbaI     XmnI     ZraI
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