Restriction map for pF1KB6184
 (Linear) (Six-base) MAPSORT of: KIBB6184.seq  Check: 9537  from: 1  to: 2100



KIBB6184.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:19  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    1974    2100 
   Size:       1974     126
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     358     947    1208    1615    2083    2100 
   Size:        358     589     261     407     468      17

  Fragments arranged by size: 

                 589     468     407     358     261      17
 
AleI CACnn'nnGTG

Cuts at:      0    1429    2100 
   Size:       1429     671
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     148     180    2100 
   Size:        148      32    1920

  Fragments arranged by size: 

                1920     148      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0    1368    1985    2052    2100 
   Size:       1368     617      67      48

  Fragments arranged by size: 

                1368     617      67      48
 
ApaI G_GGCC'C

Cuts at:      0     132    2100 
   Size:        132    1968
 
ApoI r'AATT_y

Cuts at:      0     814     979    2100 
   Size:        814     165    1121

  Fragments arranged by size: 

                1121     814     165
 
AvaI C'yCGr_G

Cuts at:      0     616    1693    1918    2100 
   Size:        616    1077     225     182

  Fragments arranged by size: 

                1077     616     225     182
 
BanI G'GyrC_C

Cuts at:      0    1092    2100 
   Size:       1092    1008
 
BanII G_rGCy'C

Cuts at:      0     132     617     767     806    1201    1411    1732    2100 
   Size:        132     485     150      39     395     210     321     368

  Fragments arranged by size: 

                 485     395     368     321     210     150     132      39
            
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     275     661    1883    1996    2100 
   Size:        275     386    1222     113     104

  Fragments arranged by size: 

                1222     386     275     113     104
 
BbvCI CC'TCA_GC

Cuts at:      0     124    2100 
   Size:        124    1976
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1173    1207    2100 
   Size:       1173      34     893

  Fragments arranged by size: 

                1173     893      34
 
BglI GCCn_nnn'nGGC

Cuts at:      0    1121    2100 
   Size:       1121     979
 
BglII A'GATC_T

Cuts at:      0     777    2100 
   Size:        777    1323
 
Bme1580I G_kGCm'C

Cuts at:      0     132     645    1097    2100 
   Size:        132     513     452    1003

  Fragments arranged by size: 

                1003     513     452     132
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     589    1091    1356    1423    2100 
   Size:        589     502     265      67     677

  Fragments arranged by size: 

                 677     589     502     265      67
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1428    2100 
   Size:       1428     672
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     619     631    1423    1597    1642    2100 
   Size:        619      12     792     174      45     458

  Fragments arranged by size: 

                 792     619     458     174      45      12
 
Bpu10I CC'TnA_GC

Cuts at:      0     124    1141    2100 
   Size:        124    1017     959

  Fragments arranged by size: 

                1017     959     124
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     588    1213    2100 
   Size:        588     625     887

  Fragments arranged by size: 

                 887     625     588
 
BsaBI GATnn'nnATC

Cuts at:      0    1171    2100 
   Size:       1171     929
 
BsaWI w'CCGG_w

Cuts at:      0    1745    2100 
   Size:       1745     355
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     670     700    2100 
   Size:        670      30    1400

  Fragments arranged by size: 

                1400     670      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     124     433     436     724     739     886    1639    2100 
   Size:        124     309       3     288      15     147     753     461

  Fragments arranged by size: 

                 753     461     309     288     147     124      15       3
            
 
BseYI C'CCAG_C

Cuts at:      0     348     456     467     644    1180    2068    2100 
   Size:        348     108      11     177     536     888      32

  Fragments arranged by size: 

                 888     536     348     177     108      32      11
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      22    1054    1450    1455    1471    2100 
   Size:         22    1032     396       5      16     629

  Fragments arranged by size: 

                1032     629     396      22      16       5
 
BsiHKAI G_wGCw'C

Cuts at:      0     617    1229    1330    2100 
   Size:        617     612     101     770

  Fragments arranged by size: 

                 770     617     612     101
 
Bsp1286I G_dGCh'C

Cuts at:      0     132     617     645     767     806    1097    1201    1229 
   Size:        132     485      28     122      39     291     104      28

Cuts at:   1229    1330    1411    1732    2100 
   Size:        101      81     321     368

  Fragments arranged by size: 

                 485     368     321     291     132     122     104     101
                  81      39      28      28
 
BspEI T'CCGG_A

Cuts at:      0    1745    2100 
   Size:       1745     355
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    1974    2100 
   Size:       1974     126
 
BsrDI GCAATG_nn'

Cuts at:      0     208    2100 
   Size:        208    1892
 
BsrFI r'CCGG_y

Cuts at:      0     344    2100 
   Size:        344    1756
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     437    1065    2100 
   Size:        437     628    1035

  Fragments arranged by size: 

                1035     628     437
 
BstEII G'GTnAC_C

Cuts at:      0    1452    2100 
   Size:       1452     648
 
BstYI r'GATC_y

Cuts at:      0      24     777    2100 
   Size:         24     753    1323

  Fragments arranged by size: 

                1323     753      24
 
Bsu36I CC'TnA_GG

Cuts at:      0     392     992    2100 
   Size:        392     600    1108

  Fragments arranged by size: 

                1108     600     392
 
BtgI C'CryG_G

Cuts at:      0     253     650    1532    2100 
   Size:        253     397     882     568

  Fragments arranged by size: 

                 882     568     397     253
 
BtsI GCAGTG_nn'

Cuts at:      0     319     364     794    1462    1801    1937    2100 
   Size:        319      45     430     668     339     136     163

  Fragments arranged by size: 

                 668     430     339     319     163     136      45
 
DraIII CAC_nnn'GTG

Cuts at:      0     453    2100 
   Size:        453    1647
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     277    2100 
   Size:        277    1823
 
EaeI y'GGCC_r

Cuts at:      0     170    1122    2100 
   Size:        170     952     978

  Fragments arranged by size: 

                 978     952     170
 
EarI CTCTTCn'nnn_

Cuts at:      0    1237    1699    1851    2100 
   Size:       1237     462     152     249

  Fragments arranged by size: 

                1237     462     249     152
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0      24    1074    1740    2100 
   Size:         24    1050     666     360

  Fragments arranged by size: 

                1050     666     360      24
 
EcoICRI GAG'CTC

Cuts at:      0     615    2100 
   Size:        615    1485
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     358     619     631     947    1208    1423    1597    1615 
   Size:        358     261      12     316     261     215     174      18

Cuts at:   1615    1642    2083    2100 
   Size:         27     441      17

  Fragments arranged by size: 

                 441     358     316     261     261     215     174      27
                  18      17      12
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     244     991    1624    2100 
   Size:        244     747     633     476

  Fragments arranged by size: 

                 747     633     476     244
 
EcoO109I rG'GnC_Cy

Cuts at:      0     128     129    2100 
   Size:        128       1    1971

  Fragments arranged by size: 

                1971     128       1
 
EcoRI G'AATT_C

Cuts at:      0     814    2100 
   Size:        814    1286
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     985    1017    2100 
   Size:        985      32    1083

  Fragments arranged by size: 

                1083     985      32
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     929    2100 
   Size:        929    1171
 
MscI TGG'CCA

Cuts at:      0     172    1124    2100 
   Size:        172     952     976

  Fragments arranged by size: 

                 976     952     172
 
MslI CAynn'nnrTG

Cuts at:      0    1429    2100 
   Size:       1429     671
 
MspA1I CmG'CkG

Cuts at:      0     123     467     907    1038    2100 
   Size:        123     344     440     131    1062

  Fragments arranged by size: 

                1062     440     344     131     123
 
NaeI GCC'GGC

Cuts at:      0     346    2100 
   Size:        346    1754
 
NcoI C'CATG_G

Cuts at:      0     253    1532    2100 
   Size:        253    1279     568

  Fragments arranged by size: 

                1279     568     253
 
NgoMIV G'CCGG_C

Cuts at:      0     344    2100 
   Size:        344    1756
 
NspI r_CATG'y

Cuts at:      0    1501    2100 
   Size:       1501     599
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    1446    1985    2100 
   Size:       1446     539     115

  Fragments arranged by size: 

                1446     539     115
 
PfoI T'CCnGG_A

Cuts at:      0    1295    2100 
   Size:       1295     805
 
PspOMI G'GGCC_C

Cuts at:      0     128    2100 
   Size:        128    1972
 
PspXI vC'TCGA_Gb

Cuts at:      0     616    2100 
   Size:        616    1484
 
PstI C_TGCA'G

Cuts at:      0    1008    2100 
   Size:       1008    1092
 
PvuII CAG'CTG

Cuts at:      0     123    1038    2100 
   Size:        123     915    1062

  Fragments arranged by size: 

                1062     915     123
 
SacI G_AGCT'C

Cuts at:      0     617    2100 
   Size:        617    1483
 
ScaI AGT'ACT

Cuts at:      0    1528    2100 
   Size:       1528     572
 
SfcI C'TryA_G

Cuts at:      0    1004    2100 
   Size:       1004    1096
 
SmlI C'TyrA_G

Cuts at:      0     567     616    1228    2100 
   Size:        567      49     612     872

  Fragments arranged by size: 

                 872     612     567      49
 
StyI C'CwwG_G

Cuts at:      0     253     806    1532    2100 
   Size:        253     553     726     568

  Fragments arranged by size: 

                 726     568     553     253
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     430     864    1120    2100 
   Size:        430     434     256     980

  Fragments arranged by size: 

                 980     434     430     256
 
TatI w'GTAC_w

Cuts at:      0     209     599    1526    2100 
   Size:        209     390     927     574

  Fragments arranged by size: 

                 927     574     390     209
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0      70     102    1233    1265    2100 
   Size:         70      32    1131      32     835

  Fragments arranged by size: 

                1131     835      70      32      32
 
Tth111I GACn'n_nGTC

Cuts at:      0     241    2100 
   Size:        241    1859
 
XhoI C'TCGA_G

Cuts at:      0     616    2100 
   Size:        616    1484
 

 Enzymes that do cut:

     AarI     AcuI     AleI     AloI    AlwNI     ApaI     ApoI     AvaI
     BanI    BanII     BbsI    BbvCI     BcgI     BglI    BglII Bme1580I
     BmrI     BplI     BpmI   Bpu10I    BpuEI    BsaBI    BsaWI    BsaXI
    BseRI    BseYI     BsgI  BsiHKAI Bsp1286I    BspEI    BspMI    BsrDI
    BsrFI   BstAPI   BstEII    BstYI   Bsu36I     BtgI     BtsI   DraIII
     DrdI     EaeI     EarI     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I
    EcoRI    Hin4I     MmeI     MscI     MslI   MspA1I     NaeI     NcoI
   NgoMIV     NspI    PflMI     PfoI   PspOMI    PspXI     PstI    PvuII
     SacI     ScaI     SfcI     SmlI     StyI    TaqII     TatI     TstI
  Tth111I     XhoI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII     AgeI
     AhdI     AlfI    ApaLI     AscI     AseI    AsiSI    AvrII     BaeI
    BamHI     BbeI    BciVI     BclI    BfrBI     BlpI    BmgBI     BmtI
     BsaI    BsaAI    BsaHI    BsiEI    BsiWI     BsmI    BsmBI    BspHI
    BsrBI    BsrGI   BssHII    BssSI    BstBI    BstXI  BstZ17I    BtgZI
     ClaI    CspCI     DraI     EagI    EcoRV     FalI     FseI     FspI
    FspAI    HaeII   HincII  HindIII     HpaI     KasI     KpnI     MfeI
     MluI     NarI     NdeI     NheI     NotI     NruI     NsiI     PacI
     PasI     PciI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI
     PsrI     PvuI    RsrII    SacII     SalI    SanDI     SapI     SbfI
    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI
     SphI     SrfI     SspI     StuI     SwaI     XbaI     XcmI     XmaI
     XmnI     ZraI
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