Restriction map for pF1KB8203
 (Linear) (Six-base) MAPSORT of: KIBB8203.seq  Check: 2480  from: 1  to: 909



KIBB8203.orf

 With 182 enzymes: SgfI * 

                        March 2, 2010 14:28  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     788     909 
   Size:        788     121
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     232     909 
   Size:        232     677
 
AfeI AGC'GCT

Cuts at:      0     411     436     909 
   Size:        411      25     473

  Fragments arranged by size: 

                 473     411      25
 
AhdI GACnn_n'nnGTC

Cuts at:      0     273     909 
   Size:        273     636
 
AleI CACnn'nnGTG

Cuts at:      0     554     909 
   Size:        554     355
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     824     856     909 
   Size:        824      32      53

  Fragments arranged by size: 

                 824      53      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0     237     384     909 
   Size:        237     147     525

  Fragments arranged by size: 

                 525     237     147
 
ApaI G_GGCC'C

Cuts at:      0     585     909 
   Size:        585     324
 
AscI GG'CGCG_CC

Cuts at:      0     252     909 
   Size:        252     657
 
AvaI C'yCGr_G

Cuts at:      0      58      69     909 
   Size:         58      11     840

  Fragments arranged by size: 

                 840      58      11
 
BanII G_rGCy'C

Cuts at:      0      70      86     585     820     909 
   Size:         70      16     499     235      89

  Fragments arranged by size: 

                 499     235      89      70      16
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0      82     116     750     784     909 
   Size:         82      34     634      34     125

  Fragments arranged by size: 

                 634     125      82      34      34
 
BglI GCCn_nnn'nGGC

Cuts at:      0     589     909 
   Size:        589     320
 
Bme1580I G_kGCm'C

Cuts at:      0     585     909 
   Size:        585     324
 
BmgBI CAC'GTC

Cuts at:      0     576     909 
   Size:        576     333
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     734     909 
   Size:        734     175
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     501     909 
   Size:        501     408
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     280     334     480     909 
   Size:        280      54     146     429

  Fragments arranged by size: 

                 429     280     146      54
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      70     909 
   Size:         70     839
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      28     466     787     909 
   Size:         28     438     321     122

  Fragments arranged by size: 

                 438     321     122      28
 
BseYI C'CCAG_C

Cuts at:      0      36     406     584     893     909 
   Size:         36     370     178     309      16

  Fragments arranged by size: 

                 370     309     178      36      16
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     466     909 
   Size:        466     443
 
BsiEI CG_ry'CG

Cuts at:      0     150     759     909 
   Size:        150     609     150

  Fragments arranged by size: 

                 609     150     150
 
BsiHKAI G_wGCw'C

Cuts at:      0      86     779     909 
   Size:         86     693     130

  Fragments arranged by size: 

                 693     130      86
 
BsmI GAATG_Cn'

Cuts at:      0     874     909 
   Size:        874      35
 
Bsp1286I G_dGCh'C

Cuts at:      0      70      86     585     779     820     909 
   Size:         70      16     499     194      41      89

  Fragments arranged by size: 

                 499     194      89      70      41      16
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     788     909 
   Size:        788     121
 
BsrBI CCG'CTC

Cuts at:      0     456     909 
   Size:        456     453
 
BsrDI GCAATG_nn'

Cuts at:      0     503     909 
   Size:        503     406
 
BsrFI r'CCGG_y

Cuts at:      0     248     909 
   Size:        248     661
 
BssHII G'CGCG_C

Cuts at:      0      74     252     909 
   Size:         74     178     657

  Fragments arranged by size: 

                 657     178      74
 
BssSI C'ACGA_G

Cuts at:      0     226     550     909 
   Size:        226     324     359

  Fragments arranged by size: 

                 359     324     226
 
BstBI TT'CG_AA

Cuts at:      0     417     909 
   Size:        417     492
 
BtgI C'CryG_G

Cuts at:      0     509     909 
   Size:        509     400
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     324     909 
   Size:        324     585
 
BtsI GCAGTG_nn'

Cuts at:      0     310     909 
   Size:        310     599
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     574     909 
   Size:        574     335
 
EaeI y'GGCC_r

Cuts at:      0     147     176     239     368     909 
   Size:        147      29      63     129     541

  Fragments arranged by size: 

                 541     147     129      63      29
 
EagI C'GGCC_G

Cuts at:      0     147     909 
   Size:        147     762
 
EarI CTCTTCn'nnn_

Cuts at:      0      58     419     527     655     909 
   Size:         58     361     108     128     254

  Fragments arranged by size: 

                 361     254     128     108      58
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0      65     640     909 
   Size:         65     575     269

  Fragments arranged by size: 

                 575     269      65
 
EcoICRI GAG'CTC

Cuts at:      0      84     909 
   Size:         84     825
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     232     280     334     480     909 
   Size:        232      48      54     146     429

  Fragments arranged by size: 

                 429     232     146      54      48
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     370     826     909 
   Size:        370     456      83

  Fragments arranged by size: 

                 456     370      83
 
EcoO109I rG'GnC_Cy

Cuts at:      0     234     909 
   Size:        234     675
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     306     723     909 
   Size:        306     417     186

  Fragments arranged by size: 

                 417     306     186
 
HaeII r_GCGC'y

Cuts at:      0      44     413     438     909 
   Size:         44     369      25     471

  Fragments arranged by size: 

                 471     369      44      25
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     259     291     909 
   Size:        259      32     618

  Fragments arranged by size: 

                 618     259      32
 
MscI TGG'CCA

Cuts at:      0     370     909 
   Size:        370     539
 
MslI CAynn'nnrTG

Cuts at:      0     554     909 
   Size:        554     355
 
MspA1I CmG'CkG

Cuts at:      0     171     312     381     511     909 
   Size:        171     141      69     130     398

  Fragments arranged by size: 

                 398     171     141     130      69
 
NaeI GCC'GGC

Cuts at:      0     250     909 
   Size:        250     659
 
NgoMIV G'CCGG_C

Cuts at:      0     248     909 
   Size:        248     661
 
NruI TCG'CGA

Cuts at:      0     424     909 
   Size:        424     485
 
PspOMI G'GGCC_C

Cuts at:      0     581     909 
   Size:        581     328
 
PstI C_TGCA'G

Cuts at:      0     353     785     909 
   Size:        353     432     124

  Fragments arranged by size: 

                 432     353     124
 
PvuII CAG'CTG

Cuts at:      0     312     909 
   Size:        312     597
 
SacI G_AGCT'C

Cuts at:      0      86     909 
   Size:         86     823
 
SacII CC_GC'GG

Cuts at:      0     512     909 
   Size:        512     397
 
SapI GCTCTTCn'nnn_

Cuts at:      0      58     419     909 
   Size:         58     361     490

  Fragments arranged by size: 

                 490     361      58
 
SfcI C'TryA_G

Cuts at:      0     349     781     909 
   Size:        349     432     128

  Fragments arranged by size: 

                 432     349     128
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0     589     909 
   Size:        589     320
 
SmlI C'TyrA_G

Cuts at:      0      85     909 
   Size:         85     824
 
StuI AGG'CCT

Cuts at:      0     592     909 
   Size:        592     317
 
StyI C'CwwG_G

Cuts at:      0     371     909 
   Size:        371     538
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     885     909 
   Size:        885      24
 
TatI w'GTAC_w

Cuts at:      0     617     909 
   Size:        617     292
 

 Enzymes that do cut:

     AarI     AcuI     AfeI     AhdI     AleI     AloI    AlwNI     ApaI
     AscI     AvaI    BanII     BcgI     BglI Bme1580I    BmgBI     BmrI
     BplI     BpmI    BpuEI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI
     BsmI Bsp1286I    BspMI    BsrBI    BsrDI    BsrFI   BssHII    BssSI
    BstBI     BtgI    BtgZI     BtsI     DrdI     EaeI     EagI     EarI
     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I     FalI    HaeII    Hin4I
     MscI     MslI   MspA1I     NaeI   NgoMIV     NruI   PspOMI     PstI
    PvuII     SacI    SacII     SapI     SfcI     SfiI     SmlI     StuI
     StyI    TaqII     TatI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI    AflII   AflIII     AgeI     AlfI
    ApaLI     ApoI     AseI    AsiSI    AvrII     BaeI    BamHI     BanI
     BbeI     BbsI    BbvCI    BciVI     BclI    BfrBI    BglII     BlpI
     BmtI   Bpu10I     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI
    BsiWI    BsmBI    BspEI    BspHI    BsrGI   BstAPI   BstEII    BstXI
    BstYI  BstZ17I   Bsu36I     ClaI    CspCI     DraI   DraIII    EcoRI
    EcoRV     FseI     FspI    FspAI   HincII  HindIII     HpaI     KasI
     KpnI     MfeI     MluI     MmeI     NarI     NcoI     NdeI     NheI
     NotI     NsiI     NspI     PacI     PasI     PciI    PflMI     PfoI
     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI    PspXI     PsrI
     PvuI    RsrII     SalI    SanDI     SbfI     ScaI    SexAI     SfoI
     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI     SspI
     SwaI     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI
     ZraI
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