Restriction map for pF1KB5684
##################################
# NO CUT ENZYMES (  119 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AflII       AflIII      AgeI        AhdI        AocI        
Aor51HI     AscI        AseI        AsnI        Asp700I     
Asp718I     AspEI       AspI        AviII       AvrII       
BalI        BamHI       BbvCI       BciVI       BclI        
BfrI        BglI        BlnI        BlpI        Bpu1102I    
BsaBI       BsaI        BsaWI       BseAI       BsiWI       
BsmBI       BsmI        BspDI       BspEI       BspHI       
BspLU11I    BsrFI       BssHII      BssSI       Bst1107I    
BstBI       BstZ17I     Bsu36I      CelII       Cfr10I      
ClaI        CpoI        DraI        DrdI        EagI        
Eam1105I    EclXI       Eco47III    Eco52I      Eco81I      
EcoNI       EcoRV       EcoT22I     FbaI        FseI        
FspI        HincII      HindII      HindIII     HpaI        
KpnI        KspI        MamI        MfeI        MluI        
MluNI       MroI        MscI        MslI        MunI        
NaeI        NcoI        NdeI        NgoMIV      NheI        
NotI        NruI        NsiI        NspV        PacI        
PaeR7I      PflFI       PinAI       PmeI        PpuMI       
PshAI       PshBI       Psp1406I    PvuI        RcaI        
RsrII       SacII       SalI        SbfI        SexAI       
SfiI        SfuI        SgrAI       SmlI        SnaBI       
SpeI        SphI        Sse8387I    SwaI        Tth111I     
XbaI        XcmI        XhoI        XmnI        


###################################
# ONE CUT ENZYMES (   43 enzymes) #
###################################

AccII         462  CGCG                 CG^CG
Alw44I        441  GTGCAC               G^TGCAC
AlwI          726  GATCC                GGATC(4/5)
ApaI         1498  GGGCCC               GGGCC^C
ApaLI         441  GTGCAC               G^TGCAC
AvaI         1626  CCCGGG               C^[CT]CG[GA]G
BbeI          433  GGCGCC               GGCGC^C
BbsI           52  GTCTTC               GAAGAC(2/6)
BfaI         1657  CTAG                 C^TAG
BglII         103  AGATCT               A^GATCT
BpmI         1478  CTGGAG               CTGGAG(16/14)
BpuAI          52  GTCTTC               GAAGAC(2/6)
BsgI         1425  CTGCAC               GTGCAG(16/14)
BsiEI        1681  CGACCG               CG[GA][CT]^CG
BsoBI        1626  CCCGGG               C^[CT]CG[GA]G
Bsp1407I     1634  TGTACA               T^GTACA
BsrGI        1634  TGTACA               T^GTACA
BstAPI       1280  GCACTGTGTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII       1644  GGTTACC              G^GT[ACGT]ACC
BstPI        1644  GGTTACC              G^GT[ACGT]ACC
BstUI         462  CGCG                 CG^CG
BstXI         503  CCATCAGGCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI         103  AGATCT               [GA]^GATC[CT]
EaeI         1566  TGGCCG               [CT]^GGCC[GA]
EcoO65I      1644  GGTTACC              G^GT[ACGT]ACC
HaeII         433  GGCGCC               [GA]GCGC^[CT]
HgaI         1519  GACGC                GACGC(5/10)
KasI          433  GGCGCC               G^GCGCC
MaeI         1657  CTAG                 C^TAG
MflI          103  AGATCT               [GA]^GATC[CT]
MvnI          462  CGCG                 CG^CG
NarI          433  GGCGCC               GG^CGCC
SacI         1333  GAGCTC               GAGCT^C
SapI          942  GCTCTTC              GCTCTTC(1/4)
ScaI          866  AGTACT               AGT^ACT
SfoI          433  GGCGCC               GGC^GCC
SmaI         1626  CCCGGG               CCC^GGG
SspBI        1634  TGTACA               T^GTACA
SspI          200  AATATT               AAT^ATT
StuI         1358  AGGCCT               AGG^CCT
XhoII         103  AGATCT               [GA]^GATC[CT]
XmaCI        1626  CCCGGG               C^CCGGG
XmaI         1626  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   16 enzymes) #
###################################

AcyI          433  GGCGCC               G[GA]^CG[CT]C
             1519  GACGCC              
BcgI          333  CGAAGGATTTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
              690  CGAGTCGGGTGC        
BsaHI         433  GGCGCC               G[GA]^CG[CT]C
             1519  GACGCC              
BsrDI        1218  CATTGC               GCAATG(2/0)
                7  GCAATG              
DraIII        235  CACTCAGTG            CAC[ACGT][ACGT][ACGT]^GTG
             1281  CACTGTGTG           
DsaI          669  CCGTGG               C^C[GA][CT]GG
              712  CCGTGG              
EcoRI         717  GAATTC               G^AATTC
             1189  GAATTC              
EcoT14I       265  CCAAGG               C^C[AT][AT]GG
             1640  CCAAGG              
Hin1I         433  GGCGCC               G[GA]^CG[CT]C
             1519  GACGCC              
MseI          578  TTAA                 T^TAA
             1445  TTAA                
NspI         1466  ACATGC               [GA]CATG^[CT]
             1676  ACATGC              
PflMI         768  CCAGGCACTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              821  CCAAGCCCTGG         
PleI          553  GACTC                GAGTC(4/5)
              691  GAGTC               
StyI          265  CCAAGG               C^C[AT][AT]GG
             1640  CCAAGG              
Tru9I         578  TTAA                 T^TAA
             1445  TTAA                
Van91I        768  CCAGGCACTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              821  CCAAGCCCTGG         


#########################################
# 3 OR MORE CUT ENZYMES (   80 enzymes) #
#########################################

AciI        AcsI        AfaI        AluI        AlwNI       
ApoI        AspHI       AvaII       BanI        BanII       
BbrPI       BbvI        BcnI        BmyI        BsaAI       
BsaJI       BseRI       BsiHKAI     BsiYI       BslI        
BsmAI       BsmFI       Bsp1286I    BspMI       BsrBI       
BsrI        BssKI       BstF5I      BstNI       Cac8I       
CfoI        Cfr13I      DdeI        DpnII       DraII       
EarI        EcoO109I    EcoRII      FauI        Fnu4HI      
FokI        HaeIII      HapII       HhaI        HinP1I      
HinfI       HpaII       HphI        ItaI        Ksp632I     
MaeII       MaeIII      MboI        MboII       MnlI        
MspA1I      MspI        MvaI        MwoI        NciI        
NdeII       NlaIII      NlaIV       PmaCI       PmlI        
PstI        PvuII       RsaI        Sau3AI      Sau96I      
ScrFI       SfaNI       SfcI        TaqI        TfiI        
TseI        Tsp45I      Tsp509I     TspRI       TthHB8I     
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com