Restriction map for pF1KE5606
 (Circular) (Six-base) MAPSORT of: pF1KE5606.seq  Check: 6261  from: 1  to: 4646



pF1KE5606  4646 bp

 With 182 enzymes: SgfI * 

                        February 27, 2017 10:28  ..

AccI GT'mk_AC

Cuts at:   1684    1684 
   Size:       4646
 
Acc65I G'GTAC_C

Cuts at:   1662    1662 
   Size:       4646
 
AclI AA'CG_TT

Cuts at:   4486    4486 
   Size:       4646
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1622    2471    2903    3650    3918    1622 
   Size:        849     432     747     268    2350

  Fragments arranged by size: 

                2350     849     747     432     268
 
AfeI AGC'GCT

Cuts at:   1028    1028 
   Size:       4646
 
AflIII A'CryG_T

Cuts at:    755    1998    3102    4079     755 
   Size:       1243    1104     977    1322

  Fragments arranged by size: 

                1322    1243    1104     977
 
AgeI A'CCGG_T

Cuts at:   3034    3034 
   Size:       4646
 
AlwNI CAG_nnn'CTG

Cuts at:    494     812    1951    3518     494 
   Size:        318    1139    1567    1622

  Fragments arranged by size: 

                1622    1567    1139     318
 
ApaLI G'TGCA_C

Cuts at:   3416    3416 
   Size:       4646
 
ApoI r'AATT_y

Cuts at:    385     916    1550    1650    3999    4255     385 
   Size:        531     634     100    2349     256     776

  Fragments arranged by size: 

                2349     776     634     531     256     100
 
AseI AT'TA_AT

Cuts at:     20     449    1268    1526      20 
   Size:        429     819     258    3140

  Fragments arranged by size: 

                3140     819     429     258
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       4646
 
AvaI C'yCGr_G

Cuts at:   1666    1666 
   Size:       4646
 
BamHI G'GATC_C

Cuts at:   1671    1671 
   Size:       4646
 
BanI G'GyrC_C

Cuts at:   1316    1662    2076    2324    2359    1316 
   Size:        346     414     248      35    3603

  Fragments arranged by size: 

                3603     414     346     248      35
 
BanII G_rGCy'C

Cuts at:    153    1660    2690     153 
   Size:       1507    1030    2109

  Fragments arranged by size: 

                2109    1507    1030
 
BbeI G_GCGC'C

Cuts at:   1320    2080    2328    1320 
   Size:        760     248    3638

  Fragments arranged by size: 

                3638     760     248
 
BbsI GAAGACnn'nnnn_

Cuts at:    637    1474     637 
   Size:        837    3809
 
BciVI GTATCCnnnnn_n'

Cuts at:   2544    3305    4646    2544 
   Size:        761    1341    2544

  Fragments arranged by size: 

                2544    1341     761
 
BclI T'GATC_A

Cuts at:   2166    2166 
   Size:       4646
 
BglII A'GATC_T

Cuts at:    367    2161    4198     367 
   Size:       1794    2037     815

  Fragments arranged by size: 

                2037    1794     815
 
BlpI GC'TnA_GC

Cuts at:   1758    1758 
   Size:       4646
 
Bme1580I G_kGCm'C

Cuts at:   2271    2364    3420    2271 
   Size:         93    1056    3497

  Fragments arranged by size: 

                3497    1056      93
 
BmrI ACTGGGnnnn_n'

Cuts at:    999    1305    1858    1939    2274    4418     999 
   Size:        306     553      81     335    2144    1227

  Fragments arranged by size: 

                2144    1227     553     335     306      81
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3013    3013 
   Size:       4646
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:    682     785    1094    1535     682 
   Size:        103     309     441    3793

  Fragments arranged by size: 

                3793     441     309     103
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1822    2701    3193    3491    1822 
   Size:        879     492     298    2977

  Fragments arranged by size: 

                2977     879     492     298
 
BsaI GGTCTCn'nnnn_

Cuts at:    282    4350     282 
   Size:       4068     578
 
BsaAI yAC'GTr

Cuts at:    675    1999     675 
   Size:       1324    3322
 
BsaBI GATnn'nnATC

Cuts at:      6    2160    4197       6 
   Size:       2154    2037     455

  Fragments arranged by size: 

                2154    2037     455
 
BsaHI Gr'CG_yC

Cuts at:   1317    2077    2325    3027    4523    1317 
   Size:        760     248     702    1496    1440

  Fragments arranged by size: 

                1496    1440     760     702     248
 
BsaWI w'CCGG_w

Cuts at:   1835    2356    3034    3308    3455    3866    1835 
   Size:        521     678     274     147     411    2615

  Fragments arranged by size: 

                2615     678     521     411     274     147
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:   1091    1091 
   Size:       4646
 
BseYI C'CCAG_C

Cuts at:    146    2071    2829    3406    3902     146 
   Size:       1925     758     577     496     890

  Fragments arranged by size: 

                1925     890     758     577     496
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:    497     497 
   Size:       4646
 
BsiEI CG_ry'CG

Cuts at:    104    2188    2234    3442     104 
   Size:       2084      46    1208    1308

  Fragments arranged by size: 

                2084    1308    1208      46
 
BsiHKAI G_wGCw'C

Cuts at:   1660    2438    2628    3420    1660 
   Size:        778     190     792    2886

  Fragments arranged by size: 

                2886     792     778     190
 
Bsp1286I G_dGCh'C

Cuts at:    153    1660    2271    2364    2438    2628    2690    3420     153 
   Size:       1507     611      93      74     190      62     730    1379

  Fragments arranged by size: 

                1507    1379     730     611     190      93      74      62
            
 
BspEI T'CCGG_A

Cuts at:   3866    3866 
   Size:       4646
 
BspHI T'CATG_A

Cuts at:   3822    3822 
   Size:       4646
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:   1696    1910    2212    2593    1696 
   Size:        214     302     381    3749

  Fragments arranged by size: 

                3749     381     302     214
 
BsrBI CCG'CTC

Cuts at:     50    2938    2992    4476    4641      50 
   Size:       2888      54    1484     165      55

  Fragments arranged by size: 

                2888    1484     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:   1296    1353    2558    1296 
   Size:         57    1205    3384

  Fragments arranged by size: 

                3384    1205      57
 
BsrFI r'CCGG_y

Cuts at:   2644    2825    3034    2644 
   Size:        181     209    4256

  Fragments arranged by size: 

                4256     209     181
 
BsrGI T'GTAC_A

Cuts at:   1112    1112 
   Size:       4646
 
BssSI C'ACGA_G

Cuts at:   1160    2917    3275    1160 
   Size:       1757     358    2531

  Fragments arranged by size: 

                2531    1757     358
 
BstAPI GCAn_nnn'nTGC

Cuts at:   1624    2023    1624 
   Size:        399    4247
 
BstBI TT'CG_AA

Cuts at:   3007    3007 
   Size:       4646
 
BstYI r'GATC_y

Cuts at:      1     367    1605    1671    2138    2161    2496    2742    3754 
   Size:        366    1238      66     467      23     335     246    1012

Cuts at:   3754    3840    3852    4198       1 
   Size:         86      12     346     449

  Fragments arranged by size: 

                1238    1012     467     449     366     346     335     246
                  86      66      23      12
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:    225    2596    2777    2940     225 
   Size:       2371     181     163    1931

  Fragments arranged by size: 

                2371    1931     181     163
 
BtsI GCAGTG_nn'

Cuts at:    891    1889     891 
   Size:        998    3648
 
ClaI AT'CG_AT

Cuts at:   1311    1311 
   Size:       4646
 
DraI TTT'AAA

Cuts at:   1645    1645 
   Size:       4646
 
DrdI GACnn_nn'nnGTC

Cuts at:   2352    3210    2352 
   Size:        858    3788
 
EaeI y'GGCC_r

Cuts at:    903    1183    1359    2231    2405    2796    2823    4168     903 
   Size:        280     176     872     174     391      27    1345    1381

  Fragments arranged by size: 

                1381    1345     872     391     280     176     174      27
            
 
EagI C'GGCC_G

Cuts at:   2231    2231 
   Size:       4646
 
EarI CTCTTCn'nnn_

Cuts at:   2669    2879    2669 
   Size:        210    4436
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:   2854    3164    3310    4456    2854 
   Size:        310     146    1146    3044

  Fragments arranged by size: 

                3044    1146     310     146
 
EcoICRI GAG'CTC

Cuts at:   1658    1658 
   Size:       4646
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    682     785    1094    1535    1622    2471    2903    3650    3918 
   Size:        103     309     441      87     849     432     747     268

Cuts at:   3918     682 
   Size:       1410

  Fragments arranged by size: 

                1410     849     747     441     432     309     268     103
                  87
 
EcoO109I rG'GnC_Cy

Cuts at:   1785    1785 
   Size:       4646
 
EcoRI G'AATT_C

Cuts at:    916    1650     916 
   Size:        734    3912
 
EcoRV GAT'ATC

Cuts at:    594     594 
   Size:       4646
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:    851    2665     851 
   Size:       1814    2832
 
FspI TGC'GCA

Cuts at:   2427    2427 
   Size:       4646
 
HaeII r_GCGC'y

Cuts at:   1030    1320    2080    2328    3350    4329    1030 
   Size:        290     760     248    1022     979    1347

  Fragments arranged by size: 

                1347    1022     979     760     290     248
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:    958     990    1016    1048    1438    1470    2488    2518    2520 
   Size:         32      26      32     390      32    1018      30       2

Cuts at:   2520    2550     958 
   Size:         30    3054

  Fragments arranged by size: 

                3054    1018     390      32      32      32      30      30
                  26       2
 
HincII GTy'rAC

Cuts at:   1685    1685 
   Size:       4646
 
KasI G'GCGC_C

Cuts at:   1316    2076    2324    1316 
   Size:        760     248    3638

  Fragments arranged by size: 

                3638     760     248
 
KpnI G_GTAC'C

Cuts at:   1666    1666 
   Size:       4646
 
MfeI C'AATT_G

Cuts at:   1533    1533 
   Size:       4646
 
MluI A'CGCG_T

Cuts at:   4079    4079 
   Size:       4646
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   1107    3317    3501    1107 
   Size:       2210     184    2252

  Fragments arranged by size: 

                2252    2210     184
 
MscI TGG'CCA

Cuts at:   1185    1361    2407    1185 
   Size:        176    1046    3424

  Fragments arranged by size: 

                3424    1046     176
 
MslI CAynn'nnrTG

Cuts at:    458    2635    2762     458 
   Size:       2177     127    2342

  Fragments arranged by size: 

                2342    2177     127
 
MspA1I CmG'CkG

Cuts at:   1757    2071    2431    3444    3689    1757 
   Size:        314     360    1013     245    2714

  Fragments arranged by size: 

                2714    1013     360     314     245
 
NaeI GCC'GGC

Cuts at:   2827    2827 
   Size:       4646
 
NarI GG'CG_CC

Cuts at:   1317    2077    2325    1317 
   Size:        760     248    3638

  Fragments arranged by size: 

                3638     760     248
 
NdeI CA'TA_TG

Cuts at:    455     455 
   Size:       4646
 
NgoMIV G'CCGG_C

Cuts at:   2825    2825 
   Size:       4646
 
NspI r_CATG'y

Cuts at:    759    1699    2199    3106     759 
   Size:        940     500     907    2299

  Fragments arranged by size: 

                2299     940     907     500
 
PciI A'CATG_T

Cuts at:    755    3102     755 
   Size:       2347    2299
 
PflMI CCAn_nnn'nTGG

Cuts at:    547     547 
   Size:       4646
 
PmeI GTTT'AAAC

Cuts at:   1645    1645 
   Size:       4646
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3785    3817    3785 
   Size:         32    4614
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:    474     506     474 
   Size:         32    4614
 
PstI C_TGCA'G

Cuts at:    825    1693    2378     825 
   Size:        868     685    3093

  Fragments arranged by size: 

                3093     868     685
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       4646
 
PvuII CAG'CTG

Cuts at:   2071    2431    2071 
   Size:        360    4286
 
RsrII CG'GwC_CG

Cuts at:   2841    2841 
   Size:       4646
 
SacI G_AGCT'C

Cuts at:   1660    1660 
   Size:       4646
 
SalI G'TCGA_C

Cuts at:   1683    1683 
   Size:       4646
 
SapI GCTCTTCn'nnn_

Cuts at:   2669    2879    2669 
   Size:        210    4436
 
SbfI CC_TGCA'GG

Cuts at:    825    1693     825 
   Size:        868    3778
 
ScaI AGT'ACT

Cuts at:   4163    4163 
   Size:       4646
 
SfcI C'TryA_G

Cuts at:     33     821    1689    2374    3367    3558      33 
   Size:        788     868     685     993     191    1121

  Fragments arranged by size: 

                1121     993     868     788     685     191
 
SfoI GGC'GCC

Cuts at:   1318    2078    2326    1318 
   Size:        760     248    3638

  Fragments arranged by size: 

                3638     760     248
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       4646
 
SmaI CCC'GGG

Cuts at:   1668    1668 
   Size:       4646
 
SmlI C'TyrA_G

Cuts at:   1801    2716    3208    3470    1801 
   Size:        915     492     262    2977

  Fragments arranged by size: 

                2977     915     492     262
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       4646
 
SphI G_CATG'C

Cuts at:   1699    2199    1699 
   Size:        500    4146
 
StyI C'CwwG_G

Cuts at:    322    1780    2134     322 
   Size:       1458     354    2834

  Fragments arranged by size: 

                2834    1458     354
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    853    2229    2362    2821    3030     853 
   Size:       1376     133     459     209    2469

  Fragments arranged by size: 

                2469    1376     459     209     133
 
TatI w'GTAC_w

Cuts at:   1112    4161    1112 
   Size:       3049    1597
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   4163    4195    4163 
   Size:         32    4614
 
Tth111I GACn'n_nGTC

Cuts at:   1297    2443    1297 
   Size:       1146    3500
 
XbaI T'CTAG_A

Cuts at:   1677    1677 
   Size:       4646
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:    548    1405    2141     548 
   Size:        857     736    3053

  Fragments arranged by size: 

                3053     857     736
 
XmaI C'CCGG_G

Cuts at:   1666    1666 
   Size:       4646
 

 Enzymes that do cut:

     AccI   Acc65I     AclI     AcuI     AfeI   AflIII     AgeI    AlwNI
    ApaLI     ApoI     AseI    AsiSI     AvaI    BamHI     BanI    BanII
     BbeI     BbsI    BciVI     BclI    BglII     BlpI Bme1580I     BmrI
     BplI     BpmI    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI
    BseRI    BseYI     BsgI    BsiEI  BsiHKAI Bsp1286I    BspEI    BspHI
    BspMI    BsrBI    BsrDI    BsrFI    BsrGI    BssSI   BstAPI    BstBI
    BstYI    BtgZI     BtsI     ClaI     DraI     DrdI     EaeI     EagI
     EarI     EciI  EcoICRI  Eco57MI EcoO109I    EcoRI    EcoRV     FalI
     FspI    HaeII    Hin4I   HincII     KasI     KpnI     MfeI     MluI
     MmeI     MscI     MslI   MspA1I     NaeI     NarI     NdeI   NgoMIV
     NspI     PciI    PflMI     PmeI     PpiI     PsrI     PstI     PvuI
    PvuII    RsrII     SacI     SalI     SapI     SbfI     ScaI     SfcI
     SfoI     SgfI     SmaI     SmlI     SpeI     SphI     StyI    TaqII
     TatI     TstI  Tth111I     XbaI     XcmI     XmaI

 Enzymes that do not cut: 

     AarI    AatII    AflII     AhdI     AleI     AlfI     AloI     ApaI
     AscI    AvrII     BaeI    BbvCI     BcgI    BfrBI     BglI    BmgBI
     BmtI   Bpu10I    BsaXI    BsiWI     BsmI    BsmBI   BssHII   BstEII
    BstXI  BstZ17I   Bsu36I     BtgI    CspCI   DraIII    EcoNI     FseI
    FspAI  HindIII     HpaI     NcoI     NheI     NotI     NruI     NsiI
     PacI     PasI     PfoI     PmlI    PpuMI    PshAI     PsiI   PspOMI
    PspXI    SacII    SanDI    SexAI     SfiI    SgrAI    SnaBI     SrfI
     SspI     StuI     SwaI     XhoI     XmnI     ZraI
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