Restriction map for pF1KB5920
##################################
# NO CUT ENZYMES (  106 enzymes) #
##################################

AatII       Acc65I      AclI        AcsI        AcyI        
AflII       AgeI        Alw44I      Aor51HI     ApaLI       
ApoI        AscI        AseI        AsnI        Asp718I     
AspI        AviII       AvrII       BbeI        BciVI       
BclI        BfrI        BglII       BlnI        BlpI        
Bpu1102I    BsaBI       BsaHI       BsiEI       BsiWI       
BsmBI       BspDI       BspHI       BspLU11I    BssHII      
Bst1107I    BstEII      BstPI       BstXI       BstZ17I     
CelII       ClaI        CpoI        DraI        DraIII      
DrdI        EagI        EclXI       Eco47III    Eco52I      
EcoO65I     EcoRI       EcoRV       EcoT22I     FbaI        
FseI        FspI        HaeII       Hin1I       HincII      
HindII      HpaI        KasI        KpnI        MamI        
MfeI        MluI        MseI        MunI        NaeI        
NarI        NdeI        NgoMIV      NheI        NotI        
NruI        NsiI        NspI        PacI        PaeR7I      
PflFI       PinAI       PmeI        PshAI       PshBI       
Psp1406I    PvuI        RcaI        RsrII       SalI        
SapI        ScaI        SexAI       SfiI        SfoI        
SgrAI       SnaBI       SpeI        SphI        SspI        
SwaI        TfiI        Tru9I       Tth111I     XbaI        
XhoI        


###################################
# ONE CUT ENZYMES (   47 enzymes) #
###################################

AccI         1351  GTAGAC               GT^[AC][GT]AC
AhdI          478  GACCACGAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI          56  CAGCCCCTG            CAG[ACGT][ACGT][ACGT]^CTG
AocI          106  CCTGAGG              CC^T[ACGT]AGG
ApaI          255  GGGCCC               GGGCC^C
Asp700I       546  GAAAAACTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI         478  GACCACGAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BamHI         801  GGATCC               G^GATCC
BbrPI         376  CACGTG               CAC^GTG
BbvCI        1227  CCTCAGC              CCTCAGC(-5/-2)
BcgI          806  CGAGGCACCTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI          454  CTAG                 C^TAG
BglI          761  GCCGATCTGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsmI          672  GAATGC               GAATGC(1/-1)
BsrBI         463  GAGCGG               CCGCTC(-3/-3)
BsrDI        1573  CATTGC               GCAATG(2/0)
BsrFI         148  ACCGGC               [GA]^CCGG[CT]
BstAPI        355  GCAGCAGGTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI         923  TTCGAA               TT^CGAA
Bsu36I        106  CCTGAGG              CC^T[ACGT]AGG
Cfr10I        148  ACCGGC               [GA]^CCGG[CT]
Eam1105I      478  GACCACGAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI         1003  CTCTTC               CTCTTC(1/4)
Eco81I        106  CCTGAGG              CC^T[ACGT]AGG
EcoNI        1185  CCTGGAGGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
HgaI          858  GACGC                GACGC(5/10)
HphI          855  GGTGA                GGTGA(8/7)
Ksp632I      1003  CTCTTC               CTCTTC(1/4)
KspI         1545  CCGCGG               CCGC^GG
MaeI          454  CTAG                 C^TAG
MaeIII        856  GTGAC                ^GT[ACGT]AC
NcoI         1121  CCATGG               C^CATGG
NspV          923  TTCGAA               TT^CGAA
PflMI          55  CCAGCCCCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI         376  CACGTG               CAC^GTG
PmlI          376  CACGTG               CAC^GTG
SacII        1545  CCGCGG               CCGC^GG
SbfI          335  CCTGCAGG             CCTGCA^GG
SfuI          923  TTCGAA               TT^CGAA
SmaI         1163  CCCGGG               CCC^GGG
Sse8387I      335  CCTGCAGG             CCTGCA^GG
StuI         1199  AGGCCT               AGG^CCT
Tsp45I        856  GTGAC                ^GT[GC]AC
Van91I         55  CCAGCCCCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI        1163  CCCGGG               C^CCGGG
XmaI         1163  CCCGGG               C^CCGGG
XmnI          546  GAAAAACTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   20 enzymes) #
###################################

AccIII         39  TCCGGA               T^CCGGA
              542  TCCGGA              
AflIII        377  ACGTGT               A^C[GA][CT]GT
              845  ACGTGT              
AvaI          250  CTCGGG               C^[CT]CG[GA]G
             1163  CCCGGG              
BsaI          421  GAGACC               GGTCTC(1/5)
              609  GGTCTC              
BsaWI          39  TCCGGA               [AT]^CCGG[AT]
              542  TCCGGA              
BseAI          39  TCCGGA               T^CCGGA
              542  TCCGGA              
BsoBI         250  CTCGGG               C^[CT]CG[GA]G
             1163  CCCGGG              
Bsp1407I      317  TGTACA               T^GTACA
             1025  TGTACA              
BspEI          39  TCCGGA               T^CCGGA
              542  TCCGGA              
BsrGI         317  TGTACA               T^GTACA
             1025  TGTACA              
EcoT14I       785  CCAAGG               C^C[AT][AT]GG
             1121  CCATGG              
HindIII       730  AAGCTT               A^AGCTT
              919  AAGCTT              
MroI           39  TCCGGA               T^CCGGA
              542  TCCGGA              
MslI          376  CACGTGTGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
             1581  CACGCGGGTG          
PpuMI          64  GGGACCC              [GA]G^G[AT]CC[CT]
              121  AGGTCCC             
SmlI          411  CTTGAG               C^T[CT][GA]AG
             1089  CTTGAG              
SspBI         317  TGTACA               T^GTACA
             1025  TGTACA              
StyI          785  CCAAGG               C^C[AT][AT]GG
             1121  CCATGG              
Tsp509I       246  AATT                 ^AATT
             1033  AATT                
XcmI          842  CCAACGTGTACGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
             1323  CCAGCGGCTTATTGG     


#########################################
# 3 OR MORE CUT ENZYMES (   85 enzymes) #
#########################################

AccII       AciI        AfaI        AluI        AlwI        
AspHI       AvaII       BalI        BanI        BanII       
BbsI        BbvI        BcnI        BmyI        BpmI        
BpuAI       BsaAI       BsaJI       BseRI       BsgI        
BsiHKAI     BsiYI       BslI        BsmAI       BsmFI       
Bsp1286I    BspMI       BsrI        BssKI       BssSI       
BstF5I      BstNI       BstUI       BstYI       Cac8I       
CfoI        Cfr13I      DdeI        DpnII       DraII       
DsaI        EaeI        EcoO109I    EcoRII      FauI        
Fnu4HI      FokI        HaeIII      HapII       HhaI        
HinP1I      HinfI       HpaII       ItaI        MaeII       
MboI        MboII       MflI        MluNI       MnlI        
MscI        MspA1I      MspI        MvaI        MvnI        
MwoI        NciI        NdeII       NlaIII      NlaIV       
PleI        PstI        PvuII       RsaI        SacI        
Sau3AI      Sau96I      ScrFI       SfaNI       SfcI        
TaqI        TseI        TspRI       TthHB8I     XhoII       
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