Restriction map for pF1KB5356
##################################
# NO CUT ENZYMES (  132 enzymes) #
##################################

Acc65I      AccI        AccIII      AclI        AcsI        
AflII       AflIII      AgeI        AhdI        Alw44I      
AlwI        AlwNI       AocI        Aor51HI     ApaLI       
ApoI        AscI        AseI        AsnI        Asp700I     
Asp718I     AspEI       AspI        AviII       AvrII       
BamHI       BbrPI       BbvCI       BcgI        BciVI       
BfrI        BglII       BlnI        BlpI        Bpu1102I    
BsaAI       BsaBI       BsaI        BsaWI       BseAI       
BsgI        BsiWI       BsmAI       BsmBI       Bsp1407I    
BspDI       BspEI       BspLU11I    BspMI       BsrDI       
BsrFI       BsrGI       BssHII      Bst1107I    BstBI       
BstEII      BstPI       BstYI       BstZ17I     Bsu36I      
CelII       Cfr10I      ClaI        CpoI        DraI        
Eam1105I    Eco47III    Eco81I      EcoNI       EcoO65I     
EcoRI       EcoRV       EcoT22I     FseI        FspI        
HincII      HindII      HpaI        KpnI        KspI        
MamI        MfeI        MflI        MluI        MroI        
MseI        MunI        NaeI        NdeI        NgoMIV      
NheI        NruI        NsiI        NspV        PacI        
PaeR7I      PflFI       PinAI       PmaCI       PmeI        
PmlI        PshAI       PshBI       Psp1406I    PvuI        
PvuII       RsrII       SacI        SacII       SalI        
SbfI        ScaI        SfiI        SfuI        SgrAI       
SnaBI       SpeI        SphI        Sse8387I    SspBI       
SspI        SwaI        TaqI        TfiI        Tru9I       
Tsp509I     Tth111I     TthHB8I     XbaI        XhoI        
XhoII       XmnI        


###################################
# ONE CUT ENZYMES (   31 enzymes) #
###################################

AatII          51  GACGTC               GACGT^C
BbeI          769  GGCGCC               GGCGC^C
BfaI          721  CTAG                 C^TAG
BglI           92  GCCAGGAGGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsmI          824  GAATGC               GAATGC(1/-1)
BsrBI          46  GAGCGG               CCGCTC(-3/-3)
BssSI         790  CTCGTG               CACGAG(-5/-1)
BstAPI        644  GCATGGTGTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI         458  CCATCTTCTTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DraIII        285  CACTCTGTG            CAC[ACGT][ACGT][ACGT]^GTG
DrdI          181  GACCGCTATGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
HgaI          440  GCGTC                GACGC(5/10)
HindIII      1081  AAGCTT               A^AGCTT
HinfI         976  GACTC                G^A[ACGT]TC
KasI          769  GGCGCC               G^GCGCC
MaeI          721  CTAG                 C^TAG
MaeIII        283  GTCAC                ^GT[ACGT]AC
NarI          769  GGCGCC               GG^CGCC
NotI          210  GCGGCCGC             GC^GGCCGC
NspI          258  GCATGT               [GA]CATG^[CT]
PleI          976  GACTC                GAGTC(4/5)
PpuMI         173  AGGACCC              [GA]G^G[AT]CC[CT]
PstI          679  CTGCAG               CTGCA^G
SapI          229  GAAGAGC              GCTCTTC(1/4)
SexAI        1295  ACCTGGT              A^CC[AT]GGT
SfoI          769  GGCGCC               GGC^GCC
SmaI          205  CCCGGG               CCC^GGG
SmlI          574  CTCAAG               C^T[CT][GA]AG
Tsp45I        283  GTCAC                ^GT[GC]AC
XmaCI         205  CCCGGG               C^CCGGG
XmaI          205  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   33 enzymes) #
###################################

AccII          83  CGCG                 CG^CG
              753  CGCG                
AcyI           51  GACGTC               G[GA]^CG[CT]C
              769  GGCGCC              
AfaI          483  GTAC                 GT^AC
              735  GTAC                
ApaI          108  GGGCCC               GGGCC^C
              666  GGGCCC              
AspHI         472  GTGCTC               G[AT]GC[AT]^C
              571  GTGCTC              
AvaI          205  CCCGGG               C^[CT]CG[GA]G
             1090  CTCGGG              
BalI          590  TGGCCA               TGG^CCA
             1178  TGGCCA              
BanI          769  GGCGCC               G^G[CT][GA]CC
              937  GGTGCC              
BbsI          973  GAAGAC               GAAGAC(2/6)
              522  GTCTTC              
BpuAI         973  GAAGAC               GAAGAC(2/6)
              522  GTCTTC              
BsaHI          51  GACGTC               G[GA]^CG[CT]C
              769  GGCGCC              
BsiHKAI       472  GTGCTC               G[AT]GC[AT]^C
              571  GTGCTC              
BsoBI         205  CCCGGG               C^[CT]CG[GA]G
             1090  CTCGGG              
BspHI         431  TCATGA               T^CATGA
              698  TCATGA              
BstUI          83  CGCG                 CG^CG
              753  CGCG                
FauI         1240  CCCGC                CCCGC(4/6)
             1145  GCGGG               
HapII         206  CCGG                 C^CGG
              961  CCGG                
Hin1I          51  GACGTC               G[GA]^CG[CT]C
              769  GGCGCC              
HpaII         206  CCGG                 C^CGG
              961  CCGG                
HphI          166  GGTGA                GGTGA(8/7)
              548  TCACC               
MaeII          52  ACGT                 A^CGT
             1261  ACGT                
MluNI         590  TGGCCA               TGG^CCA
             1178  TGGCCA              
MscI          590  TGGCCA               TGG^CCA
             1178  TGGCCA              
MspA1I        486  CCGCTG               C[AC]G^C[GT]G
             1143  CAGCGG              
MspI          206  CCGG                 C^CGG
              961  CCGG                
MvnI           83  CGCG                 CG^CG
              753  CGCG                
PflMI         112  CCAGAGGATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              890  CCATGGTGTGG         
RcaI          431  TCATGA               T^CATGA
              698  TCATGA              
RsaI          483  GTAC                 GT^AC
              735  GTAC                
SfaNI         531  GATGC                GCATC(5/9)
              653  GCATC               
StuI          219  AGGCCT               AGG^CCT
              686  AGGCCT              
Van91I        112  CCAGAGGATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              890  CCATGGTGTGG         
XcmI          458  CCATCTTCTTGGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
             1286  CCACGGACAACCTGG     


#########################################
# 3 OR MORE CUT ENZYMES (   62 enzymes) #
#########################################

AciI        AluI        AvaII       BanII       BbvI        
BclI        BcnI        BmyI        BpmI        BsaJI       
BseRI       BsiEI       BsiYI       BslI        BsmFI       
Bsp1286I    BsrI        BssKI       BstF5I      BstNI       
Cac8I       CfoI        Cfr13I      DdeI        DpnII       
DraII       DsaI        EaeI        EagI        EarI        
EclXI       Eco52I      EcoO109I    EcoRII      EcoT14I     
FbaI        Fnu4HI      FokI        HaeII       HaeIII      
HhaI        HinP1I      ItaI        Ksp632I     MboI        
MboII       MnlI        MslI        MvaI        MwoI        
NciI        NcoI        NdeII       NlaIII      NlaIV       
Sau3AI      Sau96I      ScrFI       SfcI        StyI        
TseI        TspRI       
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