Restriction map for pF1KB6611
##################################
# NO CUT ENZYMES (  110 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AflII       AhdI        Alw44I      AocI        Aor51HI     
ApaI        ApaLI       AscI        AseI        AsnI        
Asp718I     AspEI       AviII       AvrII       BamHI       
BbrPI       BbvCI       BcgI        BclI        BfaI        
BfrI        BlnI        BlpI        Bpu1102I    BsaBI       
BsaI        BseAI       BsiWI       BsmAI       BsmBI       
BspDI       BspEI       BspHI       BspLU11I    BsrDI       
BssHII      BssSI       Bst1107I    BstAPI      BstBI       
BstEII      BstPI       BstZ17I     Bsu36I      CelII       
ClaI        CpoI        DraIII      EagI        Eam1105I    
EclXI       Eco47III    Eco52I      Eco81I      EcoO65I     
EcoRI       EcoT14I     EcoT22I     FbaI        FseI        
FspI        HpaI        KpnI        MaeI        MamI        
MfeI        MluI        MroI        MunI        NaeI        
NcoI        NdeI        NgoMIV      NheI        NotI        
NruI        NsiI        NspV        PacI        PaeR7I      
PflMI       PmaCI       PmeI        PmlI        PshAI       
PshBI       Psp1406I    PvuI        RcaI        RsrII       
SalI        SapI        ScaI        SfiI        SfuI        
SgrAI       SnaBI       SpeI        SphI        StuI        
StyI        SwaI        Van91I      XbaI        XhoI        


###################################
# ONE CUT ENZYMES (   53 enzymes) #
###################################

AcsI            9  AAATTT               [GA]^AATT[CT]
AflIII        917  ACGTGT               A^C[GA][CT]GT
AgeI          305  ACCGGT               A^CCGGT
AlwI          159  GATCC                GGATC(4/5)
AlwNI          58  CAGGACCTG            CAG[ACGT][ACGT][ACGT]^CTG
ApoI            9  AAATTT               [GA]^AATT[CT]
Asp700I       798  GAACAGCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI         1209  GACTGAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AvaI          585  CCCGGG               C^[CT]CG[GA]G
BbeI          880  GGCGCC               GGCGC^C
BglI          677  GCCGCTGCGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII         863  AGATCT               A^GATCT
BsaWI         305  ACCGGT               [AT]^CCGG[AT]
BsiEI         870  CGACCG               CG[GA][CT]^CG
BsmI         1195  GAATGC               GAATGC(1/-1)
BsoBI         585  CCCGGG               C^[CT]CG[GA]G
BsrFI         305  ACCGGT               [GA]^CCGG[CT]
BsrI          571  ACTGG                ACTGG(1/-1)
BstXI         344  CCAACTACCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr10I        305  ACCGGT               [GA]^CCGG[CT]
DraI           12  TTTAAA               TTT^AAA
DraII          59  AGGACCT              [GA]G^G[ACGT]CC[CT]
DrdI          124  GACTTTGAAGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI         1231  CTCTTC               CTCTTC(1/4)
EcoNI         519  CCTCAACCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I       59  AGGACCT              [GA]G^G[ACGT]CC[CT]
EcoRV         197  GATATC               GAT^ATC
HincII        964  GTCAAC               GT[CT]^[GA]AC
HindII        964  GTCAAC               GT[CT]^[GA]AC
HindIII       336  AAGCTT               A^AGCTT
KasI          880  GGCGCC               G^GCGCC
Ksp632I      1231  CTCTTC               CTCTTC(1/4)
KspI          469  CCGCGG               CCGC^GG
MseI           13  TTAA                 T^TAA
NarI          880  GGCGCC               GG^CGCC
NspI         1097  ACATGC               [GA]CATG^[CT]
PflFI        1209  GACTGAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PinAI         305  ACCGGT               A^CCGGT
PpuMI          59  AGGACCT              [GA]G^G[AT]CC[CT]
PvuII          52  CAGCTG               CAG^CTG
SacII         469  CCGCGG               CCGC^GG
SbfI          987  CCTGCAGG             CCTGCA^GG
SfoI          880  GGCGCC               GGC^GCC
SmaI          585  CCCGGG               CCC^GGG
Sse8387I      987  CCTGCAGG             CCTGCA^GG
SspI          173  AATATT               AAT^ATT
Tru9I          13  TTAA                 T^TAA
Tsp509I        10  AATT                 ^AATT
Tth111I      1209  GACTGAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XcmI         1142  CCAACTTTGGCCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI         585  CCCGGG               C^CCGGG
XmaI          585  CCCGGG               C^CCGGG
XmnI          798  GAACAGCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   27 enzymes) #
###################################

AccII         163  CGCG                 CG^CG
              470  CGCG                
BalI           74  TGGCCA               TGG^CCA
              341  TGGCCA              
BbsI           39  GTCTTC               GAAGAC(2/6)
             1003  GTCTTC              
BciVI         251  GTATCC               GTATCC(6/5)
              719  GTATCC              
BpmI          572  CTGGAG               CTGGAG(16/14)
              781  CTGGAG              
BpuAI          39  GTCTTC               GAAGAC(2/6)
             1003  GTCTTC              
BsaAI         817  TACGTG               [CT]AC^GT[GA]
              907  TACGTG              
BseRI         841  GAGGAG               GAGGAG(10/8)
             1075  GAGGAG              
BsgI          598  CTGCAC               GTGCAG(16/14)
              355  GTGCAG              
BsmFI         426  GGGAC                GGGAC(10/14)
             1207  GGGAC               
BspMI         986  ACCTGC               ACCTGC(4/8)
              990  GCAGGT              
BsrBI         850  GAGCGG               CCGCTC(-3/-3)
             1024  GAGCGG              
BstUI         163  CGCG                 CG^CG
              470  CGCG                
BstYI         158  AGATCC               [GA]^GATC[CT]
              863  AGATCT              
EaeI           74  TGGCCA               [CT]^GGCC[GA]
              341  TGGCCA              
HaeII         378  GGCGCT               [GA]GCGC^[CT]
              880  GGCGCC              
MaeIII        594  GTCAC                ^GT[ACGT]AC
              977  GTGAC               
MflI          158  AGATCC               [GA]^GATC[CT]
              863  AGATCT              
MluNI          74  TGGCCA               TGG^CCA
              341  TGGCCA              
MscI           74  TGGCCA               TGG^CCA
              341  TGGCCA              
MvnI          163  CGCG                 CG^CG
              470  CGCG                
SacI          400  GAGCTC               GAGCT^C
             1078  GAGCTC              
SmlI          238  CTCAAG               C^T[CT][GA]AG
              256  CTCAAG              
TaqI          134  TCGA                 T^CGA
              639  TCGA                
Tsp45I        594  GTCAC                ^GT[GC]AC
              977  GTGAC               
TthHB8I       134  TCGA                 T^CGA
              639  TCGA                
XhoII         158  AGATCC               [GA]^GATC[CT]
              863  AGATCT              


#########################################
# 3 OR MORE CUT ENZYMES (   68 enzymes) #
#########################################

AciI        AcyI        AfaI        AluI        AspHI       
AvaII       BanI        BanII       BbvI        BcnI        
BmyI        BsaHI       BsaJI       BsiHKAI     BsiYI       
BslI        Bsp1286I    Bsp1407I    BsrGI       BssKI       
BstF5I      BstNI       Cac8I       CfoI        Cfr13I      
DdeI        DpnII       DsaI        EcoRII      FauI        
Fnu4HI      FokI        HaeIII      HapII       HgaI        
HhaI        Hin1I       HinP1I      HinfI       HpaII       
HphI        ItaI        MaeII       MboI        MboII       
MnlI        MslI        MspA1I      MspI        MvaI        
MwoI        NciI        NdeII       NlaIII      NlaIV       
PleI        PstI        RsaI        Sau3AI      Sau96I      
ScrFI       SexAI       SfaNI       SfcI        SspBI       
TfiI        TseI        TspRI       
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