Restriction map for pF1KB6580
##################################
# NO CUT ENZYMES (  171 enzymes) #
##################################

AatII       Acc65I      AccII       AccIII      AclI        
AcyI        AflII       AflIII      AgeI        AhdI        
Alw44I      AlwNI       AocI        Aor51HI     ApaI        
ApaLI       AscI        AseI        AsnI        Asp718I     
AspEI       AspHI       AspI        AvaI        AviII       
AvrII       BalI        BamHI       BanI        BanII       
BbeI        BbrPI       BcgI        BciVI       BcnI        
BfrI        BglI        BlnI        BlpI        BmyI        
BpmI        Bpu1102I    BsaAI       BsaHI       BsaI        
BsaJI       BsaWI       BseAI       BsiEI       BsiHKAI     
BsiWI       BsmBI       BsmI        BsoBI       Bsp1286I    
Bsp1407I    BspEI       BspHI       BspLU11I    BspMI       
BsrBI       BsrDI       BsrFI       BsrGI       BssHII      
BssSI       BstAPI      BstBI       BstEII      BstPI       
BstUI       BstXI       Bsu36I      CelII       Cfr10I      
CpoI        DraIII      DrdI        DsaI        EaeI        
EagI        Eam1105I    EarI        EclXI       Eco47III    
Eco52I      Eco81I      EcoNI       EcoO65I     EcoRI       
EcoT14I     EcoT22I     FauI        FseI        FspI        
HapII       HgaI        Hin1I       HincII      HindII      
HindIII     HpaI        HpaII       KasI        KpnI        
Ksp632I     KspI        MaeII       MfeI        MluI        
MluNI       MroI        MscI        MslI        MspA1I      
MspI        MunI        MvnI        NaeI        NarI        
NciI        NcoI        NdeI        NgoMIV      NotI        
NruI        NsiI        NspI        NspV        PacI        
PaeR7I      PflFI       PflMI       PinAI       PmaCI       
PmeI        PmlI        PshAI       PshBI       Psp1406I    
PvuI        PvuII       RcaI        RsrII       SacI        
SacII       SalI        SapI        SbfI        SexAI       
SfiI        SfoI        SfuI        SgrAI       SmaI        
SmlI        SnaBI       SpeI        SphI        Sse8387I    
SspBI       StuI        StyI        SwaI        Tth111I     
Van91I      XbaI        XcmI        XhoI        XmaCI       
XmaI        


###################################
# ONE CUT ENZYMES (   43 enzymes) #
###################################

AccI          133  GTATAC               GT^[AC][GT]AC
AlwI         1156  GATCC                GGATC(4/5)
Asp700I       601  GAAACTATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BbvCI         730  CCTCAGC              CCTCAGC(-5/-2)
BbvI          358  GCAGC                GCAGC(8/12)
BglII         318  AGATCT               A^GATCT
BseRI        1054  GAGGAG               GAGGAG(10/8)
BsgI          923  GTGCAG               GTGCAG(16/14)
BsmAI         482  GAGAC                GTCTC(1/5)
BsmFI         384  GGGAC                GGGAC(10/14)
BspDI         898  ATCGAT               AT^CGAT
BssKI         841  CCAGG                ^CC[ACGT]GG
Bst1107I      133  GTATAC               GTA^TAC
BstF5I         54  GGATG                GGATG(2/0)
BstNI         841  CCAGG                CC^[AT]GG
BstYI         318  AGATCT               [GA]^GATC[CT]
BstZ17I       133  GTATAC               GTA^TAC
CfoI          516  GCGC                 GCG^C
ClaI          898  ATCGAT               AT^CGAT
DraI          435  TTTAAA               TTT^AAA
EcoRII        841  CCAGG                ^CC[AT]GG
Fnu4HI        358  GCAGC                GC^[ACGT]GC
FokI           54  GGATG                GGATG(9/13)
HaeII         515  GGCGCT               [GA]GCGC^[CT]
HaeIII        450  GGCC                 GG^CC
HhaI          516  GCGC                 GCG^C
HinP1I        516  GCGC                 G^CGC
ItaI          358  GCAGC                GC^[ACGT]GC
MflI          318  AGATCT               [GA]^GATC[CT]
MvaI          841  CCAGG                CC^[AT]GG
NheI         1075  GCTAGC               G^CTAGC
PleI         1150  GACTC                GAGTC(4/5)
PpuMI         687  AGGACCC              [GA]G^G[AT]CC[CT]
PstI          113  CTGCAG               CTGCA^G
ScaI          948  AGTACT               AGT^ACT
ScrFI         841  CCAGG                CC^[ACGT]GG
SfaNI         789  GATGC                GCATC(5/9)
SfcI          113  CTGCAG               C^T[GA][CT]AG
SspI          145  AATATT               AAT^ATT
TseI          358  GCAGC                G^C[AT]GC
Tsp45I       1109  GTGAC                ^GT[GC]AC
XhoII         318  AGATCT               [GA]^GATC[CT]
XmnI          601  GAAACTATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   15 enzymes) #
###################################

AciI          233  CCGC                 CCGC(-3/-1)
              623  GCGG                
BbsI          271  GAAGAC               GAAGAC(2/6)
              703  GAAGAC              
BclI          888  TGATCA               T^GATCA
             1122  TGATCA              
BpuAI         271  GAAGAC               GAAGAC(2/6)
              703  GAAGAC              
BsaBI         343  GATGACAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             1177  GATGAAGATC          
DraII         449  AGGCCCT              [GA]G^G[ACGT]CC[CT]
              687  AGGACCC             
EcoO109I      449  AGGCCCT              [GA]G^G[ACGT]CC[CT]
              687  AGGACCC             
EcoRV          44  GATATC               GAT^ATC
              754  GATATC              
FbaI          888  TGATCA               T^GATCA
             1122  TGATCA              
MamI          343  GATGACAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             1177  GATGAAGATC          
MwoI          442  GCACAAGAGGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
              623  GCGGAACTTGC         
NlaIII        352  CATG                 CATG^
              714  CATG                
NlaIV         688  GGACCC               GG[ACGT]^[ACGT]CC
             1056  GGAGCC              
TaqI           62  TCGA                 T^CGA
              899  TCGA                
TthHB8I        62  TCGA                 T^CGA
              899  TCGA                


#########################################
# 3 OR MORE CUT ENZYMES (   29 enzymes) #
#########################################

AcsI        AfaI        AluI        ApoI        AvaII       
BfaI        BsiYI       BslI        BsrI        Cac8I       
Cfr13I      DdeI        DpnII       HinfI       HphI        
MaeI        MaeIII      MboI        MboII       MnlI        
MseI        NdeII       RsaI        Sau3AI      Sau96I      
TfiI        Tru9I       Tsp509I     TspRI       
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