Restriction map for pF1KB6420
##################################
# NO CUT ENZYMES (  141 enzymes) #
##################################

AatII       Acc65I      AccIII      AclI        AcyI        
AflII       AflIII      AgeI        AhdI        Alw44I      
AocI        Aor51HI     ApaI        ApaLI       AscI        
AseI        AsnI        Asp718I     AspEI       AspHI       
AspI        AviII       BalI        BamHI       BbeI        
BbrPI       BbsI        BciVI       BclI        BfrI        
BglI        BglII       BpuAI       BsaAI       BsaHI       
BsaI        BseAI       BseRI       BsgI        BsiEI       
BsiHKAI     BsiWI       BsmBI       BsmFI       BsmI        
Bsp1407I    BspDI       BspEI       BspHI       BspLU11I    
BspMI       BsrBI       BsrGI       BssHII      BssSI       
Bst1107I    BstAPI      BstEII      BstPI       BstXI       
BstZ17I     Bsu36I      CfoI        ClaI        CpoI        
DraI        DraII       DraIII      DrdI        EaeI        
EagI        Eam1105I    EclXI       Eco47III    Eco52I      
Eco81I      EcoO109I    EcoO65I     EcoT22I     FbaI        
FseI        FspI        HaeII       HhaI        Hin1I       
HinP1I      HindIII     HpaI        KasI        KpnI        
MaeII       MfeI        MluI        MluNI       MroI        
MscI        MslI        MunI        NaeI        NarI        
NdeI        NgoMIV      NheI        NotI        NruI        
NsiI        NspI        PacI        PflFI       PflMI       
PinAI       PmaCI       PmeI        PmlI        PpuMI       
PshAI       PshBI       Psp1406I    PstI        PvuI        
RcaI        RsrII       SacI        SalI        SapI        
SbfI        ScaI        SexAI       SfcI        SfiI        
SfoI        SgrAI       SnaBI       SpeI        SphI        
Sse8387I    SspBI       SwaI        TfiI        Tth111I     
Van91I      


###################################
# ONE CUT ENZYMES (   57 enzymes) #
###################################

AccI           56  GTAGAC               GT^[AC][GT]AC
AccII          29  CGCG                 CG^CG
AcsI          874  GAATTC               [GA]^AATT[CT]
AfaI          231  GTAC                 GT^AC
AlwNI          61  CAGTTACTG            CAG[ACGT][ACGT][ACGT]^CTG
ApoI          874  GAATTC               [GA]^AATT[CT]
AvaII         138  GGACC                G^G[AT]CC
AvrII         985  CCTAGG               C^CTAGG
BanI          240  GGTGCC               G^G[CT][GA]CC
BbvCI           7  GCTGAGG              CCTCAGC(-5/-2)
BcgI          306  GCAGCGGGCTCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BlnI          985  CCTAGG               C^CTAGG
BlpI          889  GCTCAGC              GC^T[ACGT]AGC
BpmI          713  CTCCAG               CTGGAG(16/14)
Bpu1102I      889  GCTCAGC              GC^T[ACGT]AGC
BsaBI         600  GATTGATATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI         840  ACCGGA               [AT]^CCGG[AT]
BsrFI          97  GCCGGT               [GA]^CCGG[CT]
BstBI          46  TTCGAA               TT^CGAA
BstUI          29  CGCG                 CG^CG
BstYI         647  AGATCC               [GA]^GATC[CT]
CelII         889  GCTCAGC              GC^T[ACGT]AGC
Cfr10I         97  GCCGGT               [GA]^CCGG[CT]
Cfr13I        138  GGACC                G^G[ACGT]CC
EcoNI         144  CCTTGAGAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI         874  GAATTC               G^AATTC
EcoRV         604  GATATC               GAT^ATC
HaeIII        710  GGCC                 GG^CC
HgaI          291  GCGTC                GACGC(5/10)
HincII        277  GTCAAC               GT[CT]^[GA]AC
HindII        277  GTCAAC               GT[CT]^[GA]AC
HinfI         664  GACTC                G^A[ACGT]TC
KspI           28  CCGCGG               CCGC^GG
MamI          600  GATTGATATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI          647  AGATCC               [GA]^GATC[CT]
MseI          337  TTAA                 T^TAA
MvnI           29  CGCG                 CG^CG
NcoI          425  CCATGG               C^CATGG
NspV           46  TTCGAA               TT^CGAA
PaeR7I        314  CTCGAG               C^TCGAG
PleI          664  GACTC                GAGTC(4/5)
PvuII          22  CAGCTG               CAG^CTG
RsaI          231  GTAC                 GT^AC
SacII          28  CCGCGG               CCGC^GG
Sau96I        138  GGACC                G^G[ACGT]CC
SfuI           46  TTCGAA               TT^CGAA
SmaI          953  CCCGGG               CCC^GGG
SspI          572  AATATT               AAT^ATT
StuI          709  AGGCCT               AGG^CCT
Tru9I         337  TTAA                 T^TAA
Tsp45I        803  GTGAC                ^GT[GC]AC
XbaI          294  TCTAGA               T^CTAGA
XcmI          416  CCAATTCCTCCATGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI          314  CTCGAG               C^TCGAG
XhoII         647  AGATCC               [GA]^GATC[CT]
XmaCI         953  CCCGGG               C^CCGGG
XmaI          953  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (    9 enzymes) #
###################################

AlwI          648  GATCC                GGATC(4/5)
              843  GGATC               
Asp700I       120  GAAGGTGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
              720  GAAACCCTTC          
BsmAI         662  GAGAC                GTCTC(1/5)
              440  GTCTC               
BsrDI         349  CATTGC               GCAATG(2/0)
              520  GCAATG              
DsaI           28  CCGCGG               C^C[GA][CT]GG
              425  CCATGG              
FauI           94  CCCGC                CCCGC(4/6)
              309  GCGGG               
SfaNI         454  GCATC                GCATC(5/9)
              679  GCATC               
Tsp509I       418  AATT                 ^AATT
              875  AATT                
XmnI          120  GAAGGTGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
              720  GAAACCCTTC          


#########################################
# 3 OR MORE CUT ENZYMES (   51 enzymes) #
#########################################

AciI        AluI        AvaI        BanII       BbvI        
BcnI        BfaI        BmyI        BsaJI       BsiYI       
BslI        BsoBI       Bsp1286I    BsrI        BssKI       
BstF5I      BstNI       Cac8I       DdeI        DpnII       
EarI        EcoRII      EcoT14I     Fnu4HI      FokI        
HapII       HpaII       HphI        ItaI        Ksp632I     
MaeI        MaeIII      MboI        MboII       MnlI        
MspA1I      MspI        MvaI        MwoI        NciI        
NdeII       NlaIII      NlaIV       Sau3AI      ScrFI       
SmlI        StyI        TaqI        TseI        TspRI       
TthHB8I     
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