Restriction map for pF1KB6420
 (Linear) (Six-base) MAPSORT of: KIBB6420.seq  Check: 8692  from: 1  to: 1017



KIBB6420.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:05  ..

AccI GT'mk_AC

Cuts at:      0      57    1017 
   Size:         57     960
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     247     574    1017 
   Size:        247     327     443

  Fragments arranged by size: 

                 443     327     247
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     109     141     926     958    1017 
   Size:        109      32     785      32      59

  Fragments arranged by size: 

                 785     109      59      32      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0      66    1017 
   Size:         66     951
 
ApoI r'AATT_y

Cuts at:      0     874    1017 
   Size:        874     143
 
AvaI C'yCGr_G

Cuts at:      0      37     314     953    1017 
   Size:         37     277     639      64

  Fragments arranged by size: 

                 639     277      64      37
 
AvrII C'CTAG_G

Cuts at:      0     985    1017 
   Size:        985      32
 
BanI G'GyrC_C

Cuts at:      0     240    1017 
   Size:        240     777
 
BanII G_rGCy'C

Cuts at:      0     261     315     954    1017 
   Size:        261      54     639      63

  Fragments arranged by size: 

                 639     261      63      54
 
BbvCI CC'TCA_GC

Cuts at:      0       8    1017 
   Size:          8    1009
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     295     329    1017 
   Size:        295      34     688

  Fragments arranged by size: 

                 688     295      34
 
BlpI GC'TnA_GC

Cuts at:      0     890    1017 
   Size:        890     127
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     698    1017 
   Size:        698     319
 
Bpu10I CC'TnA_GC

Cuts at:      0       8    1017 
   Size:          8    1009
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     166     734    1017 
   Size:        166     568     283

  Fragments arranged by size: 

                 568     283     166
 
BsaBI GATnn'nnATC

Cuts at:      0     604    1017 
   Size:        604     413
 
BsaWI w'CCGG_w

Cuts at:      0     840    1017 
   Size:        840     177
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     109     139     496     526     926     956    1017 
   Size:        109      30     357      30     400      30      61

  Fragments arranged by size: 

                 400     357     109      61      30      30      30
 
BseYI C'CCAG_C

Cuts at:      0     283    1017 
   Size:        283     734
 
Bsp1286I G_dGCh'C

Cuts at:      0     261     315     954    1017 
   Size:        261      54     639      63

  Fragments arranged by size: 

                 639     261      63      54
 
BsrDI GCAATG_nn'

Cuts at:      0     348     527    1017 
   Size:        348     179     490

  Fragments arranged by size: 

                 490     348     179
 
BsrFI r'CCGG_y

Cuts at:      0      97    1017 
   Size:         97     920
 
BstBI TT'CG_AA

Cuts at:      0      47    1017 
   Size:         47     970
 
BstYI r'GATC_y

Cuts at:      0     647    1017 
   Size:        647     370
 
BtgI C'CryG_G

Cuts at:      0      28     425    1017 
   Size:         28     397     592

  Fragments arranged by size: 

                 592     397      28
 
BtsI GCAGTG_nn'

Cuts at:      0     410    1017 
   Size:        410     607
 
EarI CTCTTCn'nnn_

Cuts at:      0     200     611     672     917    1017 
   Size:        200     411      61     245     100

  Fragments arranged by size: 

                 411     245     200     100      61
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     247     574     698    1017 
   Size:        247     327     124     319

  Fragments arranged by size: 

                 327     319     247     124
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     148    1017 
   Size:        148     869
 
EcoRI G'AATT_C

Cuts at:      0     874    1017 
   Size:        874     143
 
EcoRV GAT'ATC

Cuts at:      0     606    1017 
   Size:        606     411
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     683    1017 
   Size:        683     334
 
HincII GTy'rAC

Cuts at:      0     279    1017 
   Size:        279     738
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     482    1017 
   Size:        482     535
 
MspA1I CmG'CkG

Cuts at:      0      24      30     309    1017 
   Size:         24       6     279     708

  Fragments arranged by size: 

                 708     279      24       6
 
NcoI C'CATG_G

Cuts at:      0     425    1017 
   Size:        425     592
 
PfoI T'CCnGG_A

Cuts at:      0     103     113    1017 
   Size:        103      10     904

  Fragments arranged by size: 

                 904     103      10
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     109     141     926     958    1017 
   Size:        109      32     785      32      59

  Fragments arranged by size: 

                 785     109      59      32      32
 
PsiI TTA'TAA

Cuts at:      0     328    1017 
   Size:        328     689
 
PvuII CAG'CTG

Cuts at:      0      24    1017 
   Size:         24     993
 
SacII CC_GC'GG

Cuts at:      0      31    1017 
   Size:         31     986
 
SmaI CCC'GGG

Cuts at:      0     955    1017 
   Size:        955      62
 
SmlI C'TyrA_G

Cuts at:      0     145     314     749    1017 
   Size:        145     169     435     268

  Fragments arranged by size: 

                 435     268     169     145
 
SspI AAT'ATT

Cuts at:      0     574    1017 
   Size:        574     443
 
StuI AGG'CCT

Cuts at:      0     711    1017 
   Size:        711     306
 
StyI C'CwwG_G

Cuts at:      0     425     857     985    1017 
   Size:        425     432     128      32

  Fragments arranged by size: 

                 432     425     128      32
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     994    1017 
   Size:        994      23
 
XbaI T'CTAG_A

Cuts at:      0     294    1017 
   Size:        294     723
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     423    1017 
   Size:        423     594
 
XhoI C'TCGA_G

Cuts at:      0     314    1017 
   Size:        314     703
 
XmaI C'CCGG_G

Cuts at:      0     953    1017 
   Size:        953      64
 
XmnI GAAnn'nnTTC

Cuts at:      0     124     724    1017 
   Size:        124     600     293

  Fragments arranged by size: 

                 600     293     124
 

 Enzymes that do cut:

     AccI     AcuI     AloI    AlwNI     ApoI     AvaI    AvrII     BanI
    BanII    BbvCI     BcgI     BlpI     BpmI   Bpu10I    BpuEI    BsaBI
    BsaWI    BsaXI    BseYI Bsp1286I    BsrDI    BsrFI    BstBI    BstYI
     BtgI     BtsI     EarI  Eco57MI    EcoNI    EcoRI    EcoRV     FalI
   HincII     MmeI   MspA1I     NcoI     PfoI     PpiI     PsiI    PvuII
    SacII     SmaI     SmlI     SspI     StuI     StyI    TaqII     XbaI
     XcmI     XhoI     XmaI     XmnI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AfeI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI     ApaI    ApaLI     AscI     AseI    AsiSI
     BaeI    BamHI     BbeI     BbsI    BciVI     BclI    BfrBI     BglI
    BglII Bme1580I    BmgBI     BmrI     BmtI     BplI     BsaI    BsaAI
    BsaHI    BseRI     BsgI    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI
    BspEI    BspHI    BspMI    BsrBI    BsrGI   BssHII    BssSI   BstAPI
   BstEII    BstXI  BstZ17I   Bsu36I    BtgZI     ClaI    CspCI     DraI
   DraIII     DrdI     EaeI     EagI     EciI  EcoICRI EcoO109I     FseI
     FspI    FspAI    HaeII    Hin4I  HindIII     HpaI     KasI     KpnI
     MfeI     MluI     MscI     MslI     NaeI     NarI     NdeI   NgoMIV
     NheI     NotI     NruI     NsiI     NspI     PacI     PasI     PciI
    PflMI     PmeI     PmlI    PpuMI    PshAI   PspOMI    PspXI     PsrI
     PstI     PvuI    RsrII     SacI     SalI    SanDI     SapI     SbfI
     ScaI    SexAI     SfcI     SfiI     SfoI     SgfI    SgrAI    SnaBI
     SpeI     SphI     SrfI     SwaI     TatI     TstI  Tth111I     ZraI
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com