Restriction map for
pF1KB6357
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# NO CUT ENZYMES ( 158 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcyI AfaI AflII AgeI AhdI
Alw44I AlwNI Aor51HI ApaI ApaLI
AscI AseI AsnI Asp718I AspEI
AspI AvaII AviII AvrII BamHI
BanI BbeI BbrPI BbsI BbvCI
BcgI BclI BcnI BfrI BglI
BglII BlnI BlpI BpmI Bpu1102I
BpuAI BsaAI BsaBI BsaHI BsaWI
BseAI BsgI BsiEI BsiWI BsmBI
Bsp1407I BspEI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstXI BstZ17I CelII
Cfr10I CpoI DraII DraIII DrdI
DsaI EagI Eam1105I EarI EclXI
Eco47III Eco52I EcoO109I EcoRI EcoRV
EcoT14I EcoT22I FbaI FseI FspI
HaeII HapII HgaI Hin1I HincII
HindII HindIII HpaI HpaII KasI
KpnI Ksp632I KspI MamI MboII
MfeI MluI MroI MspI MunI
NaeI NarI NciI NcoI NdeI
NgoMIV NheI NlaIV NotI NruI
NsiI NspV PacI PflFI PinAI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PstI PvuI PvuII
RsaI RsrII SacII SalI SapI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
StyI SwaI TfiI Tth111I XbaI
XcmI XmaCI XmaI
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# ONE CUT ENZYMES ( 44 enzymes) #
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AccII 476 CGCG CG^CG
AflIII 401 ACATGT A^C[GA][CT]GT
AocI 61 CCTCAGG CC^T[ACGT]AGG
Asp700I 782 GAAGTAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 482 GAGCTC G[AT]GC[AT]^C
AvaI 350 CTCGAG C^[CT]CG[GA]G
BalI 648 TGGCCA TGG^CCA
BanII 482 GAGCTC G[GA]GC[CT]^C
BciVI 696 GTATCC GTATCC(6/5)
BfaI 610 CTAG C^TAG
BmyI 482 GAGCTC G[AGT]GC[ACT]^C
BsaI 553 GGTCTC GGTCTC(1/5)
BsiHKAI 482 GAGCTC G[AT]GC[AT]^C
BsmI 758 GCATTC GAATGC(1/-1)
BsoBI 350 CTCGAG C^[CT]CG[GA]G
Bsp1286I 482 GAGCTC G[AGT]GC[ACT]^C
BspDI 377 ATCGAT AT^CGAT
BspLU11I 401 ACATGT A^CATGT
BsrI 211 ACTGG ACTGG(1/-1)
BstEII 324 GGTCACC G^GT[ACGT]ACC
BstPI 324 GGTCACC G^GT[ACGT]ACC
BstUI 476 CGCG CG^CG
Bsu36I 61 CCTCAGG CC^T[ACGT]AGG
ClaI 377 ATCGAT AT^CGAT
EaeI 648 TGGCCA [CT]^GGCC[GA]
Eco81I 61 CCTCAGG CC^T[ACGT]AGG
EcoNI 544 CCTGGGACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 324 GGTCACC G^GT[ACGT]ACC
FauI 70 GCGGG CCCGC(4/6)
MaeI 610 CTAG C^TAG
MaeII 230 ACGT A^CGT
MaeIII 325 GTCAC ^GT[ACGT]AC
MluNI 648 TGGCCA TGG^CCA
MscI 648 TGGCCA TGG^CCA
MslI 571 CACTTCGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 55 CCGCTG C[AC]G^C[GT]G
MvnI 476 CGCG CG^CG
MwoI 475 GCGCGAGGAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NspI 401 ACATGT [GA]CATG^[CT]
PaeR7I 350 CTCGAG C^TCGAG
SacI 482 GAGCTC GAGCT^C
Tsp45I 325 GTCAC ^GT[GC]AC
XhoI 350 CTCGAG C^TCGAG
XmnI 782 GAAGTAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AcsI 190 AAATTC [GA]^AATT[CT]
726 AAATTT
AluI 86 AGCT AG^CT
483 AGCT
ApoI 190 AAATTC [GA]^AATT[CT]
726 AAATTT
BsaJI 544 CCTGGG C^C[ACGT][ACGT]GG
740 CCCAGG
BseRI 479 GAGGAG GAGGAG(10/8)
560 GAGGAG
BsmAI 353 GAGAC GTCTC(1/5)
554 GTCTC
BspHI 461 TCATGA T^CATGA
639 TCATGA
BstYI 416 AGATCC [GA]^GATC[CT]
717 AGATCC
Cac8I 29 GCCTGC GC[ACGT]^[ACGT]GC
525 GCTGGC
CfoI 475 GCGC GCG^C
541 GCGC
Cfr13I 48 GGCCC G^G[ACGT]CC
511 GGGCC
DraI 396 TTTAAA TTT^AAA
730 TTTAAA
HhaI 475 GCGC GCG^C
541 GCGC
HinP1I 475 GCGC G^CGC
541 GCGC
HphI 8 TCACC GGTGA(8/7)
326 TCACC
MflI 416 AGATCC [GA]^GATC[CT]
717 AGATCC
RcaI 461 TCATGA T^CATGA
639 TCATGA
Sau96I 48 GGCCC G^G[ACGT]CC
511 GGGCC
SfaNI 183 GATGC GCATC(5/9)
565 GATGC
SfcI 88 CTACAG C^T[GA][CT]AG
330 CTACAG
XhoII 416 AGATCC [GA]^GATC[CT]
717 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 35 enzymes) #
#########################################
AciI AlwI BbvI BsiYI BslI
BsmFI BssKI BstF5I BstNI DdeI
DpnII EcoRII Fnu4HI FokI HaeIII
HinfI ItaI MboI MnlI MseI
MvaI NdeII NlaIII PflMI PleI
Sau3AI ScrFI SmlI TaqI Tru9I
TseI Tsp509I TspRI TthHB8I Van91I