Restriction map for pF1KB3649
 (Linear) (Six-base) MAPSORT of: KIBB3649.seq  Check: 772  from: 1  to: 1776



KIBB3649.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:15  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     697    1390    1451    1688    1776 
   Size:        697     693      61     237      88

  Fragments arranged by size: 

                 697     693     237      88      61
 
AlwNI CAG_nnn'CTG

Cuts at:      0     892    1090    1776 
   Size:        892     198     686

  Fragments arranged by size: 

                 892     686     198
 
ApoI r'AATT_y

Cuts at:      0    1733    1776 
   Size:       1733      43
 
AvaI C'yCGr_G

Cuts at:      0     371     850    1776 
   Size:        371     479     926

  Fragments arranged by size: 

                 926     479     371
 
BanI G'GyrC_C

Cuts at:      0     484    1776 
   Size:        484    1292
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     134     685     845    1037    1709    1776 
   Size:        134     551     160     192     672      67

  Fragments arranged by size: 

                 672     551     192     160     134      67
 
BglII A'GATC_T

Cuts at:      0     672    1227    1776 
   Size:        672     555     549

  Fragments arranged by size: 

                 672     555     549
 
BlpI GC'TnA_GC

Cuts at:      0    1394    1776 
   Size:       1394     382
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     918    1166    1776 
   Size:        918     248     610

  Fragments arranged by size: 

                 918     610     248
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     170    1776 
   Size:        170    1606
 
BsaAI yAC'GTr

Cuts at:      0     820     990    1776 
   Size:        820     170     786

  Fragments arranged by size: 

                 820     786     170
 
BsaBI GATnn'nnATC

Cuts at:      0     671    1776 
   Size:        671    1105
 
BsaWI w'CCGG_w

Cuts at:      0     842    1563    1776 
   Size:        842     721     213

  Fragments arranged by size: 

                 842     721     213
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     135     138     165     168    1264    1294    1776 
   Size:        135       3      27       3    1096      30     482

  Fragments arranged by size: 

                1096     482     135      30      27       3       3
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     143     791    1046    1633    1636    1711    1738    1776 
   Size:        143     648     255     587       3      75      27      38

  Fragments arranged by size: 

                 648     587     255     143      75      38      27       3
            
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     583    1776 
   Size:        583    1193
 
BspHI T'CATG_A

Cuts at:      0      60    1776 
   Size:         60    1716
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     908    1533    1776 
   Size:        908     625     243

  Fragments arranged by size: 

                 908     625     243
 
BstYI r'GATC_y

Cuts at:      0     672     882    1227    1776 
   Size:        672     210     345     549

  Fragments arranged by size: 

                 672     549     345     210
 
Bsu36I CC'TnA_GG

Cuts at:      0    1016    1776 
   Size:       1016     760
 
BtgI C'CryG_G

Cuts at:      0    1441    1776 
   Size:       1441     335
 
BtsI GCAGTG_nn'

Cuts at:      0     146    1776 
   Size:        146    1630
 
EarI CTCTTCn'nnn_

Cuts at:      0     113     938    1427    1504    1571    1580    1589    1628 
   Size:        113     825     489      77      67       9       9      39

Cuts at:   1628    1640    1764    1776 
   Size:         12     124      12

  Fragments arranged by size: 

                 825     489     124     113      77      67      39      12
                  12       9       9
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     170     697    1390    1451    1688    1776 
   Size:        170     527     693      61     237      88

  Fragments arranged by size: 

                 693     527     237     170      88      61
 
EcoO109I rG'GnC_Cy

Cuts at:      0     831    1365    1776 
   Size:        831     534     411

  Fragments arranged by size: 

                 831     534     411
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    1657    1776 
   Size:       1657     119
 
HaeII r_GCGC'y

Cuts at:      0    1440    1776 
   Size:       1440     336
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     860     892    1085    1117    1152    1184    1264    1296 
   Size:        860      32     193      32      35      32      80      32

Cuts at:   1296    1776 
   Size:        480

  Fragments arranged by size: 

                 860     480     193      80      35      32      32      32
                  32
 
HincII GTy'rAC

Cuts at:      0     756    1776 
   Size:        756    1020
 
HindIII A'AGCT_T

Cuts at:      0     337    1776 
   Size:        337    1439
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     217     636    1776 
   Size:        217     419    1140

  Fragments arranged by size: 

                1140     419     217
 
MslI CAynn'nnrTG

Cuts at:      0      74    1776 
   Size:         74    1702
 
MspA1I CmG'CkG

Cuts at:      0    1429    1776 
   Size:       1429     347
 
NspI r_CATG'y

Cuts at:      0     655    1776 
   Size:        655    1121
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     765    1776 
   Size:        765    1011
 
PfoI T'CCnGG_A

Cuts at:      0    1089    1776 
   Size:       1089     687
 
PmlI CAC'GTG

Cuts at:      0     820    1776 
   Size:        820     956
 
PpuMI rG'GwC_Cy

Cuts at:      0     831    1776 
   Size:        831     945
 
PsiI TTA'TAA

Cuts at:      0    1136    1776 
   Size:       1136     640
 
PvuII CAG'CTG

Cuts at:      0    1429    1776 
   Size:       1429     347
 
SapI GCTCTTCn'nnn_

Cuts at:      0    1427    1776 
   Size:       1427     349
 
SfcI C'TryA_G

Cuts at:      0    1469    1776 
   Size:       1469     307
 
SnaBI TAC'GTA

Cuts at:      0     990    1776 
   Size:        990     786
 
StyI C'CwwG_G

Cuts at:      0     167    1776 
   Size:        167    1609
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0      27     138     170    1776 
   Size:         27     111      32    1606

  Fragments arranged by size: 

                1606     111      32      27
 
Tth111I GACn'n_nGTC

Cuts at:      0     109    1776 
   Size:        109    1667
 

 Enzymes that do cut:

     AcuI    AlwNI     ApoI     AvaI     BanI     BbsI    BglII     BlpI
     BmrI     BpmI    BsaAI    BsaBI    BsaWI    BsaXI    BseRI     BsgI
    BspHI    BstXI    BstYI   Bsu36I     BtgI     BtsI     EarI  Eco57MI
 EcoO109I     FalI    HaeII    Hin4I   HincII  HindIII     MmeI     MslI
   MspA1I     NspI    PflMI     PfoI     PmlI    PpuMI     PsiI    PvuII
     SapI     SfcI    SnaBI     StyI     TstI  Tth111I

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI     ApaI    ApaLI     AscI
     AseI    AsiSI    AvrII     BaeI    BamHI    BanII     BbeI    BbvCI
     BcgI    BciVI     BclI    BfrBI     BglI Bme1580I    BmgBI     BmtI
     BplI   Bpu10I    BpuEI     BsaI    BsaHI    BseYI    BsiEI  BsiHKAI
    BsiWI     BsmI    BsmBI Bsp1286I    BspEI    BspMI    BsrBI    BsrDI
    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII  BstZ17I
    BtgZI     ClaI    CspCI     DraI   DraIII     DrdI     EaeI     EagI
     EciI  EcoICRI    EcoNI    EcoRI    EcoRV     FseI     FspI    FspAI
     HpaI     KasI     KpnI     MfeI     MluI     MscI     NaeI     NarI
     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     PacI
     PasI     PciI     PmeI     PpiI    PshAI   PspOMI    PspXI     PsrI
     PstI     PvuI    RsrII     SacI    SacII     SalI    SanDI     SbfI
     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI
     SpeI     SphI     SrfI     SspI     StuI     SwaI    TaqII     TatI
     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com