Restriction map for pF1KB5329
##################################
# NO CUT ENZYMES (  177 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AcyI        
AflII       AflIII      AgeI        AhdI        Alw44I      
AlwNI       AocI        Aor51HI     ApaI        ApaLI       
AscI        AseI        AsnI        Asp700I     Asp718I     
AspEI       AspHI       AspI        AvaI        AviII       
AvrII       BamHI       BanI        BanII       BbeI        
BbrPI       BbsI        BbvCI       BcgI        BciVI       
BclI        BcnI        BfaI        BfrI        BglI        
BglII       BlnI        BlpI        BmyI        Bpu1102I    
BpuAI       BsaAI       BsaBI       BsaHI       BsaWI       
BseAI       BseRI       BsgI        BsiEI       BsiHKAI     
BsiWI       BsmBI       BsmI        BsoBI       Bsp1286I    
Bsp1407I    BspDI       BspEI       BspLU11I    BspMI       
BsrBI       BsrFI       BsrGI       BssHII      BssSI       
Bst1107I    BstAPI      BstBI       BstYI       BstZ17I     
Bsu36I      CelII       CfoI        Cfr10I      ClaI        
CpoI        DraI        DraII       DrdI        DsaI        
EagI        Eam1105I    EarI        EclXI       Eco47III    
Eco52I      Eco81I      EcoNI       EcoO109I    EcoRV       
EcoT22I     FbaI        FseI        FspI        HaeII       
HapII       HgaI        HhaI        Hin1I       HinP1I      
HincII      HindII      HindIII     HpaI        HpaII       
KasI        KpnI        Ksp632I     KspI        MaeI        
MamI        MfeI        MflI        MluI        MroI        
MspA1I      MspI        MunI        NaeI        NarI        
NciI        NcoI        NdeI        NgoMIV      NheI        
NotI        NruI        NsiI        NspV        PacI        
PaeR7I      PflFI       PinAI       PleI        PmaCI       
PmeI        PmlI        PpuMI       PshAI       PshBI       
PstI        PvuI        PvuII       RsrII       SacI        
SacII       SalI        SapI        SbfI        ScaI        
SexAI       SfcI        SfiI        SfoI        SfuI        
SgrAI       SmaI        SmlI        SnaBI       SpeI        
Sse8387I    SspBI       SspI        StuI        SwaI        
Tth111I     XbaI        XhoI        XhoII       XmaCI       
XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   45 enzymes) #
###################################

AccII         421  CGCG                 CG^CG
AclI           37  AACGTT               AA^CGTT
AfaI           81  GTAC                 GT^AC
AluI           77  AGCT                 AG^CT
AlwI          183  GATCC                GGATC(4/5)
AvaII         450  GGACC                G^G[AT]CC
BalI          299  TGGCCA               TGG^CCA
BpmI           73  CTGGAG               CTGGAG(16/14)
BsaI           49  GAGACC               GGTCTC(1/5)
BsmAI          49  GAGAC                GTCTC(1/5)
BsmFI         205  GGGAC                GGGAC(10/14)
BspHI         179  TCATGA               T^CATGA
BsrDI         295  GCAATG               GCAATG(2/0)
BstEII        193  GGTGACC              G^GT[ACGT]ACC
BstF5I        259  CATCC                GGATG(2/0)
BstPI         193  GGTGACC              G^GT[ACGT]ACC
BstUI         421  CGCG                 CG^CG
BstXI          16  CCAGATTCCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cac8I          90  GCATGC               GC[ACGT]^[ACGT]GC
DdeI          119  CTGAG                C^T[ACGT]AG
DraIII        354  CACCCAGTG            CAC[ACGT][ACGT][ACGT]^GTG
EcoO65I       193  GGTGACC              G^GT[ACGT]ACC
EcoRI         287  GAATTC               G^AATTC
EcoT14I       186  CCAAGG               C^C[AT][AT]GG
FauI          307  GCGGG                CCCGC(4/6)
FokI          259  CATCC                GGATG(9/13)
MluNI         299  TGGCCA               TGG^CCA
MscI          299  TGGCCA               TGG^CCA
MseI          479  TTAA                 T^TAA
MslI          405  CATAGGAATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI          421  CGCG                 CG^CG
MwoI          118  GCTGAGTTGGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NlaIV         309  GGGGCC               GG[ACGT]^[ACGT]CC
NspI           90  GCATGC               [GA]CATG^[CT]
PflMI         353  CCACCCAGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Psp1406I       37  AACGTT               AA^CGTT
RcaI          179  TCATGA               T^CATGA
RsaI           81  GTAC                 GT^AC
SfaNI         223  GCATC                GCATC(5/9)
SphI           90  GCATGC               GCATG^C
StyI          186  CCAAGG               C^C[AT][AT]GG
Tru9I         479  TTAA                 T^TAA
TspRI         358  CAGTG                CA[GC]TG[ACGT][ACGT]^
Van91I        353  CCACCCAGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI           35  CCAACGTTTACTTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG


###################################
# TWO CUT ENZYMES (   21 enzymes) #
###################################

BbvI           27  GCAGC                GCAGC(8/12)
              326  GCAGC               
BsaJI         186  CCAAGG               C^C[ACGT][ACGT]GG
              446  CCCAGG              
BssKI          23  CCTGG                ^CC[ACGT]GG
              447  CCAGG               
BstNI          23  CCTGG                CC^[AT]GG
              447  CCAGG               
Cfr13I        310  GGGCC                G^G[ACGT]CC
              450  GGACC               
DpnII         183  GATC                 ^GATC
              474  GATC                
EaeI          299  TGGCCA               [CT]^GGCC[GA]
              387  TGGCCG              
EcoRII         23  CCTGG                ^CC[AT]GG
              447  CCAGG               
HphI          193  GGTGA                GGTGA(8/7)
              414  GGTGA               
MaeII          38  ACGT                 A^CGT
              467  ACGT                
MboI          183  GATC                 ^GATC
              474  GATC                
MboII         247  GAAGA                GAAGA(8/7)
              471  GAAGA               
MvaI           23  CCTGG                CC^[AT]GG
              447  CCAGG               
NdeII         183  GATC                 ^GATC
              474  GATC                
Sau3AI        183  GATC                 ^GATC
              474  GATC                
Sau96I        310  GGGCC                G^G[ACGT]CC
              450  GGACC               
ScrFI          23  CCTGG                CC^[ACGT]GG
              447  CCAGG               
TaqI          132  TCGA                 T^CGA
              213  TCGA                
TseI           27  GCAGC                G^C[AT]GC
              326  GCAGC               
Tsp45I        194  GTGAC                ^GT[GC]AC
              340  GTGAC               
TthHB8I       132  TCGA                 T^CGA
              213  TCGA                


#########################################
# 3 OR MORE CUT ENZYMES (   15 enzymes) #
#########################################

AciI        AcsI        ApoI        BsiYI       BslI        
BsrI        Fnu4HI      HaeIII      HinfI       ItaI        
MaeIII      MnlI        NlaIII      TfiI        Tsp509I     
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