Restriction map for pF1KB7975
##################################
# NO CUT ENZYMES (  108 enzymes) #
##################################

AatII       Acc65I      AccI        AclI        AcsI        
AflII       AflIII      AgeI        AocI        ApaI        
ApoI        AscI        AseI        AsnI        Asp700I     
Asp718I     AvaI        AvrII       BanII       BbrPI       
BbsI        BbvCI       BcgI        BfrI        BglI        
BglII       BlnI        BpuAI       BsaAI       BsaBI       
BsgI        BsiWI       BsmI        BsoBI       Bsp1407I    
BspDI       BspLU11I    BsrDI       BsrGI       BssSI       
Bst1107I    BstAPI      BstBI       BstEII      BstPI       
BstZ17I     Bsu36I      ClaI        CpoI        DraI        
DraIII      DrdI        EarI        Eco81I      EcoNI       
EcoO65I     EcoRI       EcoRV       FseI        HindIII     
HpaI        KpnI        Ksp632I     KspI        MamI        
MluI        MslI        NdeI        NheI        NotI        
NruI        NspV        PacI        PaeR7I      PflMI       
PinAI       PmaCI       PmeI        PmlI        PshAI       
PshBI       Psp1406I    PvuI        RsrII       SacI        
SacII       SalI        SapI        SbfI        ScaI        
SexAI       SfiI        SfuI        SgrAI       SmaI        
SnaBI       SpeI        Sse8387I    SspBI       SspI        
StuI        SwaI        Van91I      XcmI        XhoI        
XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   50 enzymes) #
###################################

AccIII        881  TCCGGA               T^CCGGA
Alw44I       1012  GTGCAC               G^TGCAC
ApaLI        1012  GTGCAC               G^TGCAC
AviII         630  TGCGCA               TGC^GCA
BalI          905  TGGCCA               TGG^CCA
BamHI         765  GGATCC               G^GATCC
BciVI         466  GTATCC               GTATCC(6/5)
BclI          842  TGATCA               T^GATCA
BfaI          499  CTAG                 C^TAG
BlpI         1170  GCTGAGC              GC^T[ACGT]AGC
Bpu1102I     1170  GCTGAGC              GC^T[ACGT]AGC
BsaI          658  GAGACC               GGTCTC(1/5)
BseAI         881  TCCGGA               T^CCGGA
BsmBI         679  GAGACG               CGTCTC(1/5)
BspEI         881  TCCGGA               T^CCGGA
BspHI         866  TCATGA               T^CATGA
BsrFI         454  GCCGGC               [GA]^CCGG[CT]
BssHII         17  GCGCGC               G^CGCGC
CelII        1170  GCTGAGC              GC^T[ACGT]AGC
Cfr10I        454  GCCGGC               [GA]^CCGG[CT]
EagI          150  CGGCCG               C^GGCCG
EclXI         150  CGGCCG               C^GGCCG
Eco52I        150  CGGCCG               C^GGCCG
EcoT22I       871  ATGCAT               ATGCA^T
FbaI          842  TGATCA               T^GATCA
FspI          630  TGCGCA               TGC^GCA
HincII        694  GTCAAC               GT[CT]^[GA]AC
HindII        694  GTCAAC               GT[CT]^[GA]AC
MaeI          499  CTAG                 C^TAG
MaeIII        556  GTCAC                ^GT[ACGT]AC
MfeI          332  CAATTG               C^AATTG
MluNI         905  TGGCCA               TGG^CCA
MroI          881  TCCGGA               T^CCGGA
MscI          905  TGGCCA               TGG^CCA
MseI          569  TTAA                 T^TAA
MunI          332  CAATTG               C^AATTG
NaeI          454  GCCGGC               GCC^GGC
NcoI         1157  CCATGG               C^CATGG
NgoMIV        454  GCCGGC               G^CCGGC
NsiI          871  ATGCAT               ATGCA^T
PleI          989  GACTC                GAGTC(4/5)
PvuII         948  CAGCTG               CAG^CTG
RcaI          866  TCATGA               T^CATGA
SmlI          205  CTCAAG               C^T[CT][GA]AG
TaqI         1019  TCGA                 T^CGA
TfiI          537  GATTC                G^A[AT]TC
Tru9I         569  TTAA                 T^TAA
Tsp45I        556  GTCAC                ^GT[GC]AC
TthHB8I      1019  TCGA                 T^CGA
XbaI          498  TCTAGA               T^CTAGA


###################################
# TWO CUT ENZYMES (   30 enzymes) #
###################################

AcyI          981  GGCGCC               G[GA]^CG[CT]C
             1228  GGCGCC              
AfaI          798  GTAC                 GT^AC
             1026  GTAC                
AhdI          133  GACCGGAGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
              343  GACTTGGTGTC         
AlwNI         771  CAGTGCCTG            CAG[ACGT][ACGT][ACGT]^CTG
             1165  CAGTGGCTG           
Aor51HI       419  AGCGCT               AGC^GCT
             1034  AGCGCT              
AspEI         133  GACCGGAGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
              343  GACTTGGTGTC         
AspI           59  GACCGCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
              166  GACCGCGTC           
BbeI          981  GGCGCC               GGCGC^C
             1228  GGCGCC              
BsaHI         981  GGCGCC               G[GA]^CG[CT]C
             1228  GGCGCC              
BseRI         223  GAGGAG               GAGGAG(10/8)
             1108  GAGGAG              
BsiEI         150  CGGCCG               CG[GA][CT]^CG
              165  CGACCG              
BstXI        1032  CCAGCGCTTTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1241  CCAGTCCCCTGG        
BstYI         765  GGATCC               [GA]^GATC[CT]
              878  AGATCC              
DsaI          291  CCACGG               C^C[GA][CT]GG
             1157  CCATGG              
Eam1105I      133  GACCGGAGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
              343  GACTTGGTGTC         
Eco47III      419  AGCGCT               AGC^GCT
             1034  AGCGCT              
Hin1I         981  GGCGCC               G[GA]^CG[CT]C
             1228  GGCGCC              
HinfI         537  GATTC                G^A[ACGT]TC
              989  GACTC               
KasI          981  GGCGCC               G^GCGCC
             1228  GGCGCC              
MflI          765  GGATCC               [GA]^GATC[CT]
              878  AGATCC              
NarI          981  GGCGCC               GG^CGCC
             1228  GGCGCC              
NspI          121  GCATGC               [GA]CATG^[CT]
              296  GCATGC              
PflFI          59  GACCGCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
              166  GACCGCGTC           
PstI          144  CTGCAG               CTGCA^G
              685  CTGCAG              
RsaI          798  GTAC                 GT^AC
             1026  GTAC                
SfcI          144  CTGCAG               C^T[GA][CT]AG
              685  CTGCAG              
SfoI          981  GGCGCC               GGC^GCC
             1228  GGCGCC              
SphI          121  GCATGC               GCATG^C
              296  GCATGC              
Tth111I        59  GACCGCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
              166  GACCGCGTC           
XhoII         765  GGATCC               [GA]^GATC[CT]
              878  AGATCC              


#########################################
# 3 OR MORE CUT ENZYMES (   70 enzymes) #
#########################################

AccII       AciI        AluI        AlwI        AspHI       
AvaII       BanI        BbvI        BcnI        BmyI        
BpmI        BsaJI       BsaWI       BsiHKAI     BsiYI       
BslI        BsmAI       BsmFI       Bsp1286I    BspMI       
BsrBI       BsrI        BssKI       BstF5I      BstNI       
BstUI       Cac8I       CfoI        Cfr13I      DdeI        
DpnII       DraII       EaeI        EcoO109I    EcoRII      
EcoT14I     FauI        Fnu4HI      FokI        HaeII       
HaeIII      HapII       HgaI        HhaI        HinP1I      
HpaII       HphI        ItaI        MaeII       MboI        
MboII       MnlI        MspA1I      MspI        MvaI        
MvnI        MwoI        NciI        NdeII       NlaIII      
NlaIV       PpuMI       Sau3AI      Sau96I      ScrFI       
SfaNI       StyI        TseI        Tsp509I     TspRI       
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