Restriction map for pF1KB7975
 (Linear) (Six-base) MAPSORT of: KIBB7975.seq  Check: 9653  from: 1  to: 1281



KIBB7975.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:25  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    1207    1281 
   Size:       1207      74
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     493     568    1179    1281 
   Size:        493      75     611     102

  Fragments arranged by size: 

                 611     493     102      75
 
AfeI AGC'GCT

Cuts at:      0     421    1036    1281 
   Size:        421     615     245

  Fragments arranged by size: 

                 615     421     245
 
AhdI GACnn_n'nnGTC

Cuts at:      0     138     348    1281 
   Size:        138     210     933

  Fragments arranged by size: 

                 933     210     138
 
AlwNI CAG_nnn'CTG

Cuts at:      0     776    1170    1281 
   Size:        776     394     111

  Fragments arranged by size: 

                 776     394     111
 
ApaLI G'TGCA_C

Cuts at:      0    1012    1281 
   Size:       1012     269
 
BamHI G'GATC_C

Cuts at:      0     765    1281 
   Size:        765     516
 
BanI G'GyrC_C

Cuts at:      0     981    1135    1228    1236    1281 
   Size:        981     154      93       8      45

  Fragments arranged by size: 

                 981     154      93      45       8
 
BbeI G_GCGC'C

Cuts at:      0     985    1232    1281 
   Size:        985     247      49

  Fragments arranged by size: 

                 985     247      49
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     477    1281 
   Size:        477     804
 
BclI T'GATC_A

Cuts at:      0     842    1281 
   Size:        842     439
 
BfrBI ATG'CAT

Cuts at:      0     873    1281 
   Size:        873     408
 
BlpI GC'TnA_GC

Cuts at:      0    1171    1281 
   Size:       1171     110
 
Bme1580I G_kGCm'C

Cuts at:      0     724     958     977    1016    1241    1281 
   Size:        724     234      19      39     225      40

  Fragments arranged by size: 

                 724     234     225      40      39      19
 
BmgBI CAC'GTC

Cuts at:      0     972    1281 
   Size:        972     309
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     764    1080    1158    1235    1281 
   Size:        764     316      78      77      46

  Fragments arranged by size: 

                 764     316      78      77      46
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     811     850     961    1024    1281 
   Size:        811      39     111      63     257

  Fragments arranged by size: 

                 811     257     111      63      39
 
Bpu10I CC'TnA_GC

Cuts at:      0     670    1281 
   Size:        670     611
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     190    1281 
   Size:        190    1091
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     652    1281 
   Size:        652     629
 
BsaHI Gr'CG_yC

Cuts at:      0     982    1229    1281 
   Size:        982     247      52

  Fragments arranged by size: 

                 982     247      52
 
BsaWI w'CCGG_w

Cuts at:      0     134     362     881    1281 
   Size:        134     228     519     400

  Fragments arranged by size: 

                 519     400     228     134
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    1138    1168    1281 
   Size:       1138      30     113

  Fragments arranged by size: 

                1138     113      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     238    1123    1281 
   Size:        238     885     158

  Fragments arranged by size: 

                 885     238     158
 
BseYI C'CCAG_C

Cuts at:      0     950    1281 
   Size:        950     331
 
BsiEI CG_ry'CG

Cuts at:      0     153     168    1281 
   Size:        153      15    1113

  Fragments arranged by size: 

                1113     153      15
 
BsiHKAI G_wGCw'C

Cuts at:      0    1016    1115    1177    1281 
   Size:       1016      99      62     104

  Fragments arranged by size: 

                1016     104      99      62
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     673    1281 
   Size:        673     608
 
Bsp1286I G_dGCh'C

Cuts at:      0     724     958     977    1016    1115    1177    1241    1281 
   Size:        724     234      19      39      99      62      64      40

  Fragments arranged by size: 

                 724     234      99      64      62      40      39      19
            
 
BspEI T'CCGG_A

Cuts at:      0     881    1281 
   Size:        881     400
 
BspHI T'CATG_A

Cuts at:      0     866    1281 
   Size:        866     415
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     617     681    1207    1281 
   Size:        617      64     526      74

  Fragments arranged by size: 

                 617     526      74      64
 
BsrBI CCG'CTC

Cuts at:      0     212     228    1143    1207    1281 
   Size:        212      16     915      64      74

  Fragments arranged by size: 

                 915     212      74      64      16
 
BsrFI r'CCGG_y

Cuts at:      0     454    1281 
   Size:        454     827
 
BssHII G'CGCG_C

Cuts at:      0      17    1281 
   Size:         17    1264
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    1039    1248    1281 
   Size:       1039     209      33

  Fragments arranged by size: 

                1039     209      33
 
BstYI r'GATC_y

Cuts at:      0     765     878    1281 
   Size:        765     113     403

  Fragments arranged by size: 

                 765     403     113
 
BtgI C'CryG_G

Cuts at:      0     291    1157    1281 
   Size:        291     866     124

  Fragments arranged by size: 

                 866     291     124
 
BtsI GCAGTG_nn'

Cuts at:      0    1263    1281 
   Size:       1263      18
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     195     230    1281 
   Size:        195      35    1051

  Fragments arranged by size: 

                1051     195      35
 
EaeI y'GGCC_r

Cuts at:      0      86     150     456     905    1281 
   Size:         86      64     306     449     376

  Fragments arranged by size: 

                 449     376     306      86      64
 
EagI C'GGCC_G

Cuts at:      0     150    1281 
   Size:        150    1131
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     342    1281 
   Size:        342     939
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     493     568     811     850     961    1024    1179    1281 
   Size:        493      75     243      39     111      63     155     102

  Fragments arranged by size: 

                 493     243     155     111     102      75      63      39
            
 
EcoO109I rG'GnC_Cy

Cuts at:      0     140     314     596     825    1281 
   Size:        140     174     282     229     456

  Fragments arranged by size: 

                 456     282     229     174     140
 
FspI TGC'GCA

Cuts at:      0     632    1281 
   Size:        632     649
 
HaeII r_GCGC'y

Cuts at:      0     423     677     985    1038    1232    1281 
   Size:        423     254     308      53     194      49

  Fragments arranged by size: 

                 423     308     254     194      53      49
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     124     156     334     366    1138    1170    1281 
   Size:        124      32     178      32     772      32     111

  Fragments arranged by size: 

                 772     178     124     111      32      32      32
 
HincII GTy'rAC

Cuts at:      0     696    1281 
   Size:        696     585
 
KasI G'GCGC_C

Cuts at:      0     981    1228    1281 
   Size:        981     247      53

  Fragments arranged by size: 

                 981     247      53
 
MfeI C'AATT_G

Cuts at:      0     332    1281 
   Size:        332     949
 
MscI TGG'CCA

Cuts at:      0     907    1281 
   Size:        907     374
 
MspA1I CmG'CkG

Cuts at:      0     149     950    1065    1281 
   Size:        149     801     115     216

  Fragments arranged by size: 

                 801     216     149     115
 
NaeI GCC'GGC

Cuts at:      0     456    1281 
   Size:        456     825
 
NarI GG'CG_CC

Cuts at:      0     982    1229    1281 
   Size:        982     247      52

  Fragments arranged by size: 

                 982     247      52
 
NcoI C'CATG_G

Cuts at:      0    1157    1281 
   Size:       1157     124
 
NgoMIV G'CCGG_C

Cuts at:      0     454    1281 
   Size:        454     827
 
NsiI A_TGCA'T

Cuts at:      0     875    1281 
   Size:        875     406
 
NspI r_CATG'y

Cuts at:      0     125     300    1281 
   Size:        125     175     981

  Fragments arranged by size: 

                 981     175     125
 
PfoI T'CCnGG_A

Cuts at:      0     788    1001    1281 
   Size:        788     213     280

  Fragments arranged by size: 

                 788     280     213
 
PpuMI rG'GwC_Cy

Cuts at:      0     140     314     596    1281 
   Size:        140     174     282     685

  Fragments arranged by size: 

                 685     282     174     140
 
PstI C_TGCA'G

Cuts at:      0     148     689    1281 
   Size:        148     541     592

  Fragments arranged by size: 

                 592     541     148
 
PvuII CAG'CTG

Cuts at:      0     950    1281 
   Size:        950     331
 
SfcI C'TryA_G

Cuts at:      0     144     685    1281 
   Size:        144     541     596

  Fragments arranged by size: 

                 596     541     144
 
SfoI GGC'GCC

Cuts at:      0     983    1230    1281 
   Size:        983     247      51

  Fragments arranged by size: 

                 983     247      51
 
SmlI C'TyrA_G

Cuts at:      0     205    1281 
   Size:        205    1076
 
SphI G_CATG'C

Cuts at:      0     125     300    1281 
   Size:        125     175     981

  Fragments arranged by size: 

                 981     175     125
 
StyI C'CwwG_G

Cuts at:      0     977    1157    1232    1281 
   Size:        977     180      75      49

  Fragments arranged by size: 

                 977     180      75      49
 
TatI w'GTAC_w

Cuts at:      0     797    1025    1281 
   Size:        797     228     256

  Fragments arranged by size: 

                 797     256     228
 
Tth111I GACn'n_nGTC

Cuts at:      0      62     169    1281 
   Size:         62     107    1112

  Fragments arranged by size: 

                1112     107      62
 
XbaI T'CTAG_A

Cuts at:      0     498    1281 
   Size:        498     783
 

 Enzymes that do cut:

     AarI     AcuI     AfeI     AhdI    AlwNI    ApaLI    BamHI     BanI
     BbeI    BciVI     BclI    BfrBI     BlpI Bme1580I    BmgBI     BmrI
     BpmI   Bpu10I    BpuEI     BsaI    BsaHI    BsaWI    BsaXI    BseRI
    BseYI    BsiEI  BsiHKAI    BsmBI Bsp1286I    BspEI    BspHI    BspMI
    BsrBI    BsrFI   BssHII    BstXI    BstYI     BtgI     BtsI    CspCI
     EaeI     EagI     EciI  Eco57MI EcoO109I     FspI    HaeII    Hin4I
   HincII     KasI     MfeI     MscI   MspA1I     NaeI     NarI     NcoI
   NgoMIV     NsiI     NspI     PfoI    PpuMI     PstI    PvuII     SfcI
     SfoI     SmlI     SphI     StyI     TatI  Tth111I     XbaI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI    AflII   AflIII     AgeI     AleI
     AlfI     AloI     ApaI     ApoI     AscI     AseI    AsiSI     AvaI
    AvrII     BaeI    BanII     BbsI    BbvCI     BcgI     BglI    BglII
     BmtI     BplI    BsaAI    BsaBI     BsgI    BsiWI     BsmI    BsrDI
    BsrGI    BssSI   BstAPI    BstBI   BstEII  BstZ17I   Bsu36I    BtgZI
     ClaI     DraI   DraIII     DrdI     EarI  EcoICRI    EcoNI    EcoRI
    EcoRV     FalI     FseI    FspAI  HindIII     HpaI     KpnI     MluI
     MmeI     MslI     NdeI     NheI     NotI     NruI     PacI     PasI
     PciI    PflMI     PmeI     PmlI     PpiI    PshAI     PsiI   PspOMI
    PspXI     PsrI     PvuI    RsrII     SacI    SacII     SalI    SanDI
     SapI     SbfI     ScaI    SexAI     SfiI     SgfI    SgrAI     SmaI
    SnaBI     SpeI     SrfI     SspI     StuI     SwaI    TaqII     TstI
     XcmI     XhoI     XmaI     XmnI     ZraI
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