Restriction map for pF1KB7034
##################################
# NO CUT ENZYMES (  128 enzymes) #
##################################

AatII       Acc65I      AclI        AcsI        AcyI        
AflII       AhdI        Alw44I      AocI        Aor51HI     
ApaLI       ApoI        AscI        AseI        AsnI        
Asp718I     AspEI       AspI        AviII       AvrII       
BamHI       BbeI        BbrPI       BbvCI       BcgI        
BfrI        BglII       BlnI        BlpI        BpmI        
Bpu1102I    BsaAI       BsaBI       BsaHI       BsaI        
BsiEI       BsiWI       BsmBI       BspDI       BsrBI       
BsrDI       BsrI        BssHII      BssSI       Bst1107I    
BstBI       BstEII      BstPI       BstXI       BstZ17I     
Bsu36I      CelII       CfoI        ClaI        CpoI        
DraI        DraII       DraIII      DrdI        EagI        
Eam1105I    EclXI       Eco47III    Eco52I      Eco81I      
EcoNI       EcoO109I    EcoO65I     EcoRI       EcoRV       
EcoT14I     EcoT22I     FseI        FspI        HaeII       
HgaI        HhaI        Hin1I       HinP1I      HindIII     
HphI        KasI        KpnI        MamI        MfeI        
MluI        MunI        NarI        NcoI        NdeI        
NheI        NotI        NruI        NsiI        NspV        
PacI        PaeR7I      PflFI       PleI        PmaCI       
PmeI        PmlI        PpuMI       PshAI       PshBI       
Psp1406I    PvuI        RsrII       SacI        SalI        
SbfI        ScaI        SexAI       SfiI        SfoI        
SfuI        SgrAI       SnaBI       SpeI        SphI        
Sse8387I    SspI        StuI        StyI        SwaI        
Tth111I     XbaI        XhoI        


###################################
# ONE CUT ENZYMES (   44 enzymes) #
###################################

AccI         1144  GTCTAC               GT^[AC][GT]AC
AccII           8  CGCG                 CG^CG
AccIII        277  TCCGGA               T^CCGGA
AflIII        554  ACATGT               A^C[GA][CT]GT
AgeI          596  ACCGGT               A^CCGGT
ApaI           74  GGGCCC               GGGCC^C
Asp700I       650  GAAGGGCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaII         979  GGACC                G^G[AT]CC
BanI          735  GGTGCC               G^G[CT][GA]CC
BbsI          829  GTCTTC               GAAGAC(2/6)
BclI          848  TGATCA               T^GATCA
BfaI         1235  CTAG                 C^TAG
BpuAI         829  GTCTTC               GAAGAC(2/6)
BseAI         277  TCCGGA               T^CCGGA
BsgI          424  CTGCAC               GTGCAG(16/14)
BsmAI         214  GTCTC                GTCTC(1/5)
BsmI          256  GCATTC               GAATGC(1/-1)
BspEI         277  TCCGGA               T^CCGGA
BspLU11I      554  ACATGT               A^CATGT
BspMI        1228  ACCTGC               ACCTGC(4/8)
BstUI           8  CGCG                 CG^CG
BstYI         114  AGATCC               [GA]^GATC[CT]
FbaI          848  TGATCA               T^GATCA
HinfI          88  GAATC                G^A[ACGT]TC
KspI            7  CCGCGG               CCGC^GG
MaeI         1235  CTAG                 C^TAG
MflI          114  AGATCC               [GA]^GATC[CT]
MroI          277  TCCGGA               T^CCGGA
MvnI            8  CGCG                 CG^CG
NaeI          405  GCCGGC               GCC^GGC
NgoMIV        405  GCCGGC               G^CCGGC
PflMI         290  CCAGCTCCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI         596  ACCGGT               A^CCGGT
PstI          536  CTGCAG               CTGCA^G
PvuII         944  CAGCTG               CAG^CTG
SacII           7  CCGCGG               CCGC^GG
SapI          893  GCTCTTC              GCTCTTC(1/4)
SmlI          715  CTCAAG               C^T[CT][GA]AG
TfiI           88  GAATC                G^A[AT]TC
Tsp45I        532  GTCAC                ^GT[GC]AC
Van91I        290  CCAGCTCCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI          674  CCATCTGGGCTTTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII         114  AGATCC               [GA]^GATC[CT]
XmnI          650  GAAGGGCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   23 enzymes) #
###################################

AlwI          115  GATCC                GGATC(4/5)
             1177  GATCC               
AspHI         460  GTGCTC               G[AT]GC[AT]^C
              769  GTGCTC              
BanII          74  GGGCCC               G[GA]GC[CT]^C
              742  GGGCTC              
BciVI        1224  GGATAC               GTATCC(6/5)
              615  GTATCC              
BglI            6  GCCGCGGCGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
              673  GCCATCTGGGC         
BsiHKAI       460  GTGCTC               G[AT]GC[AT]^C
              769  GTGCTC              
Bsp1407I      323  TGTACA               T^GTACA
              416  TGTACA              
BspHI         575  TCATGA               T^CATGA
              762  TCATGA              
BsrFI         405  GCCGGC               [GA]^CCGG[CT]
              596  ACCGGT              
BsrGI         323  TGTACA               T^GTACA
              416  TGTACA              
BstAPI         97  GCAACAAATGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
             1082  GCATAAGCTGC         
Cfr10I        405  GCCGGC               [GA]^CCGG[CT]
              596  ACCGGT              
EarI          236  CTCTTC               CTCTTC(1/4)
              894  CTCTTC              
HincII        195  GTTAAC               GT[CT]^[GA]AC
             1269  GTTAAC              
HindII        195  GTTAAC               GT[CT]^[GA]AC
             1269  GTTAAC              
HpaI          195  GTTAAC               GTT^AAC
             1269  GTTAAC              
Ksp632I       236  CTCTTC               CTCTTC(1/4)
              894  CTCTTC              
MaeII         989  ACGT                 A^CGT
             1142  ACGT                
NspI          419  ACATGC               [GA]CATG^[CT]
              554  ACATGT              
RcaI          575  TCATGA               T^CATGA
              762  TCATGA              
SfcI          536  CTGCAG               C^T[GA][CT]AG
             1146  CTACAG              
SspBI         323  TGTACA               T^GTACA
              416  TGTACA              
Tsp509I       518  AATT                 ^AATT
             1107  AATT                


#########################################
# 3 OR MORE CUT ENZYMES (   63 enzymes) #
#########################################

AciI        AfaI        AluI        AlwNI       AvaI        
BalI        BbvI        BcnI        BmyI        BsaJI       
BsaWI       BseRI       BsiYI       BslI        BsmFI       
BsoBI       Bsp1286I    BssKI       BstF5I      BstNI       
Cac8I       Cfr13I      DdeI        DpnII       DsaI        
EaeI        EcoRII      FauI        Fnu4HI      FokI        
HaeIII      HapII       HpaII       ItaI        MaeIII      
MboI        MboII       MluNI       MnlI        MscI        
MseI        MslI        MspA1I      MspI        MvaI        
MwoI        NciI        NdeII       NlaIII      NlaIV       
RsaI        Sau3AI      Sau96I      ScrFI       SfaNI       
SmaI        TaqI        Tru9I       TseI        TspRI       
TthHB8I     XmaCI       XmaI        
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