Restriction map for pF1KB6351
##################################
# NO CUT ENZYMES (  153 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcsI        AcyI        AflII       AflIII      AgeI        
AhdI        Alw44I      AlwI        Aor51HI     ApaI        
ApaLI       ApoI        AscI        AseI        AsnI        
Asp700I     Asp718I     AspEI       AspHI       AspI        
AvrII       BalI        BamHI       BanII       BbeI        
BbrPI       BbvCI       BfrI        BglI        BlnI        
BlpI        BpmI        Bpu1102I    BsaAI       BsaHI       
BsaI        BseAI       BsgI        BsiEI       BsiHKAI     
BsiWI       BsmBI       BsmI        Bsp1407I    BspDI       
BspEI       BspHI       BspLU11I    BsrBI       BsrDI       
BsrFI       BsrGI       BssHII      BssSI       Bst1107I    
BstAPI      BstBI       BstEII      BstPI       BstXI       
BstZ17I     CelII       Cfr10I      ClaI        CpoI        
DraI        DraII       DraIII      DrdI        EagI        
Eam1105I    EarI        EclXI       Eco47III    Eco52I      
EcoNI       EcoO109I    EcoO65I     EcoRI       EcoRV       
EcoT22I     FseI        Hin1I       HincII      HindII      
HpaI        KasI        KpnI        Ksp632I     MaeII       
MfeI        MluI        MluNI       MroI        MscI        
MseI        MslI        MunI        NaeI        NarI        
NcoI        NdeI        NgoMIV      NheI        NotI        
NsiI        NspV        PacI        PaeR7I      PflFI       
PflMI       PinAI       PleI        PmaCI       PmeI        
PmlI        PpuMI       PshAI       PshBI       Psp1406I    
PvuI        PvuII       RcaI        RsrII       SacI        
SalI        SapI        SexAI       SfaNI       SfiI        
SfoI        SfuI        SgrAI       SmlI        SnaBI       
SpeI        SspBI       StuI        SwaI        TaqI        
Tru9I       Tth111I     TthHB8I     Van91I      XbaI        
XcmI        XhoI        XmnI        


###################################
# ONE CUT ENZYMES (   47 enzymes) #
###################################

AlwNI         167  CAGGCTCTG            CAG[ACGT][ACGT][ACGT]^CTG
AocI          587  CCTCAGG              CC^T[ACGT]AGG
AvaII         617  GGTCC                G^G[AT]CC
AviII         253  TGCGCA               TGC^GCA
BanI          161  GGCACC               G^G[CT][GA]CC
BcgI          352  CGAAAACGCTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI         737  GGATAC               GTATCC(6/5)
BglII         623  AGATCT               A^GATCT
BmyI          160  GGGCAC               G[AGT]GC[ACT]^C
BsaWI         569  TCCGGT               [AT]^CCGG[AT]
Bsp1286I      160  GGGCAC               G[AGT]GC[ACT]^C
BspMI         131  ACCTGC               ACCTGC(4/8)
BsrI          761  CCAGT                ACTGG(1/-1)
BstF5I        495  GGATG                GGATG(2/0)
BstNI         166  CCAGG                CC^[AT]GG
BstYI         623  AGATCT               [GA]^GATC[CT]
Bsu36I        587  CCTCAGG              CC^T[ACGT]AGG
Cfr13I        617  GGTCC                G^G[ACGT]CC
DdeI          588  CTCAG                C^T[ACGT]AG
DsaI          303  CCGCGG               C^C[GA][CT]GG
EaeI           66  CGGCCA               [CT]^GGCC[GA]
Eco81I        587  CCTCAGG              CC^T[ACGT]AGG
EcoRII        166  CCAGG                ^CC[AT]GG
EcoT14I       491  CCTTGG               C^C[AT][AT]GG
FokI          495  GGATG                GGATG(9/13)
FspI          253  TGCGCA               TGC^GCA
HaeIII         67  GGCC                 GG^CC
HindIII       468  AAGCTT               A^AGCTT
HinfI         194  GAATC                G^A[ACGT]TC
HphI          750  TCACC                GGTGA(8/7)
KspI          303  CCGCGG               CCGC^GG
MflI          623  AGATCT               [GA]^GATC[CT]
MvaI          166  CCAGG                CC^[AT]GG
NlaIV         161  GGCACC               GG[ACGT]^[ACGT]CC
NruI           41  TCGCGA               TCG^CGA
NspI          362  GCATGC               [GA]CATG^[CT]
SacII         303  CCGCGG               CCGC^GG
Sau96I        617  GGTCC                G^G[ACGT]CC
SbfI          338  CCTGCAGG             CCTGCA^GG
ScaI          284  AGTACT               AGT^ACT
SphI          362  GCATGC               GCATG^C
Sse8387I      338  CCTGCAGG             CCTGCA^GG
SspI          696  AATATT               AAT^ATT
StyI          491  CCTTGG               C^C[AT][AT]GG
TfiI          194  GAATC                G^A[AT]TC
Tsp45I        368  GTCAC                ^GT[GC]AC
XhoII         623  AGATCT               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   15 enzymes) #
###################################

AluI           24  AGCT                 AG^CT
              469  AGCT                
BbsI          417  GTCTTC               GAAGAC(2/6)
              522  GTCTTC              
BclI          423  TGATCA               T^GATCA
              480  TGATCA              
BpuAI         417  GTCTTC               GAAGAC(2/6)
              522  GTCTTC              
BsaBI         424  GATCAAAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
              748  GATCACCATC          
BseRI         265  GAGGAG               GAGGAG(10/8)
              440  GAGGAG              
BsmFI         311  GGGAC                GGGAC(10/14)
              296  GTCCC               
FbaI          423  TGATCA               T^GATCA
              480  TGATCA              
HgaI          313  GACGC                GACGC(5/10)
              366  GCGTC               
MaeIII        368  GTCAC                ^GT[ACGT]AC
              710  GTTAC               
MamI          424  GATCAAAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
              748  GATCACCATC          
NlaIII        363  CATG                 CATG^
              685  CATG                
PstI          173  CTGCAG               CTGCA^G
              339  CTGCAG              
SfcI          173  CTGCAG               C^T[GA][CT]AG
              339  CTGCAG              
TspRI         276  CACTG                CA[GC]TG[ACGT][ACGT]^
              462  CACTG               


#########################################
# 3 OR MORE CUT ENZYMES (   43 enzymes) #
#########################################

AccII       AciI        AfaI        AvaI        BbvI        
BcnI        BfaI        BsaJI       BsiYI       BslI        
BsmAI       BsoBI       BssKI       BstUI       Cac8I       
CfoI        DpnII       FauI        Fnu4HI      HaeII       
HapII       HhaI        HinP1I      HpaII       ItaI        
MaeI        MboI        MboII       MnlI        MspA1I      
MspI        MvnI        MwoI        NciI        NdeII       
RsaI        Sau3AI      ScrFI       SmaI        TseI        
Tsp509I     XmaCI       XmaI        
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