Restriction map for pF1KB6351
 (Linear) (Six-base) MAPSORT of: KIBB6351.seq  Check: 2835  from: 1  to: 798



KIBB6351.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:05  ..

AlwNI CAG_nnn'CTG

Cuts at:      0     172     798 
   Size:        172     626
 
AvaI C'yCGr_G

Cuts at:      0      29     204     385     798 
   Size:         29     175     181     413

  Fragments arranged by size: 

                 413     181     175      29
 
BanI G'GyrC_C

Cuts at:      0     161     798 
   Size:        161     637
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     410     515     798 
   Size:        410     105     283

  Fragments arranged by size: 

                 410     283     105
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     341     375     798 
   Size:        341      34     423

  Fragments arranged by size: 

                 423     341      34
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     731     798 
   Size:        731      67
 
BclI T'GATC_A

Cuts at:      0     423     480     798 
   Size:        423      57     318

  Fragments arranged by size: 

                 423     318      57
 
BglII A'GATC_T

Cuts at:      0     623     798 
   Size:        623     175
 
Bme1580I G_kGCm'C

Cuts at:      0     164     798 
   Size:        164     634
 
BsaBI GATnn'nnATC

Cuts at:      0     428     752     798 
   Size:        428     324      46

  Fragments arranged by size: 

                 428     324      46
 
BsaWI w'CCGG_w

Cuts at:      0     569     798 
   Size:        569     229
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     280     455     798 
   Size:        280     175     343

  Fragments arranged by size: 

                 343     280     175
 
BseYI C'CCAG_C

Cuts at:      0      78     144     798 
   Size:         78      66     654

  Fragments arranged by size: 

                 654      78      66
 
Bsp1286I G_dGCh'C

Cuts at:      0     164     798 
   Size:        164     634
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     140     798 
   Size:        140     658
 
BstYI r'GATC_y

Cuts at:      0     623     798 
   Size:        623     175
 
Bsu36I CC'TnA_GG

Cuts at:      0     588     798 
   Size:        588     210
 
BtgI C'CryG_G

Cuts at:      0     303     798 
   Size:        303     495
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0      58     798 
   Size:         58     740
 
EaeI y'GGCC_r

Cuts at:      0      66     798 
   Size:         66     732
 
FspI TGC'GCA

Cuts at:      0     255     798 
   Size:        255     543
 
HaeII r_GCGC'y

Cuts at:      0      92     155     185     351     798 
   Size:         92      63      30     166     447

  Fragments arranged by size: 

                 447     166      92      63      30
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     571     603     798 
   Size:        571      32     195

  Fragments arranged by size: 

                 571     195      32
 
HindIII A'AGCT_T

Cuts at:      0     468     798 
   Size:        468     330
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     140     798 
   Size:        140     658
 
MspA1I CmG'CkG

Cuts at:      0      19     144     305     798 
   Size:         19     125     161     493

  Fragments arranged by size: 

                 493     161     125      19
 
NruI TCG'CGA

Cuts at:      0      43     798 
   Size:         43     755
 
NspI r_CATG'y

Cuts at:      0     366     798 
   Size:        366     432
 
PstI C_TGCA'G

Cuts at:      0     177     343     798 
   Size:        177     166     455

  Fragments arranged by size: 

                 455     177     166
 
SacII CC_GC'GG

Cuts at:      0     306     798 
   Size:        306     492
 
SbfI CC_TGCA'GG

Cuts at:      0     343     798 
   Size:        343     455
 
ScaI AGT'ACT

Cuts at:      0     286     798 
   Size:        286     512
 
SfcI C'TryA_G

Cuts at:      0     173     339     798 
   Size:        173     166     459

  Fragments arranged by size: 

                 459     173     166
 
SmaI CCC'GGG

Cuts at:      0      31     206     387     798 
   Size:         31     175     181     411

  Fragments arranged by size: 

                 411     181     175      31
 
SphI G_CATG'C

Cuts at:      0     366     798 
   Size:        366     432
 
SspI AAT'ATT

Cuts at:      0     698     798 
   Size:        698     100
 
StyI C'CwwG_G

Cuts at:      0     491     798 
   Size:        491     307
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     179     798 
   Size:        179     619
 
TatI w'GTAC_w

Cuts at:      0     284     798 
   Size:        284     514
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     253     285     398     430     601     633     798 
   Size:        253      32     113      32     171      32     165

  Fragments arranged by size: 

                 253     171     165     113      32      32      32
 
XmaI C'CCGG_G

Cuts at:      0      29     204     385     798 
   Size:         29     175     181     413

  Fragments arranged by size: 

                 413     181     175      29
 

 Enzymes that do cut:

    AlwNI     AvaI     BanI     BbsI     BcgI    BciVI     BclI    BglII
 Bme1580I    BsaBI    BsaWI    BseRI    BseYI Bsp1286I    BspMI    BstYI
   Bsu36I     BtgI    BtgZI     EaeI     FspI    HaeII    Hin4I  HindIII
     MmeI   MspA1I     NruI     NspI     PstI    SacII     SbfI     ScaI
     SfcI     SmaI     SphI     SspI     StyI    TaqII     TatI     TstI
     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AlfI     AloI     ApaI    ApaLI
     ApoI     AscI     AseI    AsiSI    AvrII     BaeI    BamHI    BanII
     BbeI    BbvCI    BfrBI     BglI     BlpI    BmgBI     BmrI     BmtI
     BplI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaHI    BsaXI
     BsgI    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI    BspEI    BspHI
    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI
   BstEII    BstXI  BstZ17I     BtsI     ClaI    CspCI     DraI   DraIII
     DrdI     EagI     EarI     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I
    EcoRI    EcoRV     FalI     FseI    FspAI   HincII     HpaI     KasI
     KpnI     MfeI     MluI     MscI     MslI     NaeI     NarI     NcoI
     NdeI   NgoMIV     NheI     NotI     NsiI     PacI     PasI     PciI
    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI
   PspOMI    PspXI     PsrI     PvuI    PvuII    RsrII     SacI     SalI
    SanDI     SapI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmlI
    SnaBI     SpeI     SrfI     StuI     SwaI  Tth111I     XbaI     XcmI
     XhoI     XmnI     ZraI
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