Restriction map for pF1KB6681
##################################
# NO CUT ENZYMES (  122 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcyI        AflII       AflIII      AgeI        AhdI        
Alw44I      AocI        ApaLI       AscI        AseI        
AsnI        Asp700I     Asp718I     AspEI       AviII       
AvrII       BanI        BbeI        BbrPI       BcgI        
BciVI       BclI        BfrI        BlnI        BsaAI       
BsaBI       BsaHI       BsaWI       BseAI       BsiWI       
BsmI        Bsp1407I    BspDI       BspEI       BspHI       
BspLU11I    BsrFI       BsrGI       BssHII      BssSI       
Bst1107I    BstBI       BstEII      BstPI       BstXI       
BstZ17I     Bsu36I      Cfr10I      ClaI        CpoI        
DraI        DraIII      Eam1105I    EarI        Eco81I      
EcoO65I     EcoRI       EcoRV       EcoT22I     FbaI        
FseI        FspI        Hin1I       HindIII     HpaI        
KasI        KpnI        Ksp632I     KspI        MamI        
MfeI        MluI        MroI        MseI        MunI        
NaeI        NarI        NdeI        NgoMIV      NheI        
NruI        NsiI        NspV        PacI        PaeR7I      
PinAI       PmaCI       PmeI        PmlI        PshAI       
PshBI       Psp1406I    PstI        PvuI        RcaI        
RsrII       SacI        SacII       SalI        SapI        
SbfI        SfcI        SfoI        SfuI        SgrAI       
SnaBI       SpeI        SphI        Sse8387I    SspBI       
SspI        SwaI        Tru9I       XbaI        XcmI        
XhoI        XmnI        


###################################
# ONE CUT ENZYMES (   45 enzymes) #
###################################

AccII         939  CGCG                 CG^CG
AcsI          376  AAATTC               [GA]^AATT[CT]
Aor51HI       869  AGCGCT               AGC^GCT
ApoI          376  AAATTC               [GA]^AATT[CT]
AspHI         692  GAGCAC               G[AT]GC[AT]^C
AspI          412  GACCTGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BamHI         563  GGATCC               G^GATCC
BbsI          270  GAAGAC               GAAGAC(2/6)
BbvCI         964  GCTGAGG              CCTCAGC(-5/-2)
BglII         356  AGATCT               A^GATCT
BpuAI         270  GAAGAC               GAAGAC(2/6)
BsaI           36  GGTCTC               GGTCTC(1/5)
BsiEI         245  CGGCCG               CG[GA][CT]^CG
BsiHKAI       692  GAGCAC               G[AT]GC[AT]^C
BsmBI         304  GAGACG               CGTCTC(1/5)
BsrBI         778  GAGCGG               CCGCTC(-3/-3)
BstAPI        753  GCAGCGGGTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI         939  CGCG                 CG^CG
DrdI          670  GACATCGAGGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI          245  CGGCCG               C^GGCCG
EclXI         245  CGGCCG               C^GGCCG
Eco47III      869  AGCGCT               AGC^GCT
Eco52I        245  CGGCCG               C^GGCCG
EcoNI         888  CCTAGCCGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
HgaI          940  GCGTC                GACGC(5/10)
HphI         1254  GGTGA                GGTGA(8/7)
MaeII         307  ACGT                 A^CGT
MvnI          939  CGCG                 CG^CG
NotI          244  GCGGCCGC             GC^GGCCGC
NspI         1064  ACATGC               [GA]CATG^[CT]
PflFI         412  GACCTGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PflMI         786  CCAGCAGGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI         1229  GACTC                GAGTC(4/5)
PpuMI         179  GGGTCCC              [GA]G^G[AT]CC[CT]
ScaI         1188  AGTACT               AGT^ACT
SexAI         413  ACCTGGT              A^CC[AT]GGT
SfiI          327  GGCCATCATGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmaI          842  CCCGGG               CCC^GGG
SmlI          509  CTCAAG               C^T[CT][GA]AG
StuI          968  AGGCCT               AGG^CCT
TfiI         1257  GAATC                G^A[AT]TC
Tth111I       412  GACCTGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
Van91I        786  CCAGCAGGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI         842  CCCGGG               C^CCGGG
XmaI          842  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   25 enzymes) #
###################################

AlwNI         191  CAGTCACTG            CAG[ACGT][ACGT][ACGT]^CTG
              604  CAGTACCTG           
ApaI          782  GGGCCC               GGGCC^C
              983  GGGCCC              
BfaI          889  CTAG                 C^TAG
             1072  CTAG                
BlpI         1003  GCTGAGC              GC^T[ACGT]AGC
             1054  GCTCAGC             
Bpu1102I     1003  GCTGAGC              GC^T[ACGT]AGC
             1054  GCTCAGC             
BsmAI         304  GAGAC                GTCTC(1/5)
               37  GTCTC               
BsrDI         201  CATTGC               GCAATG(2/0)
              294  CATTGC              
BstF5I        102  CATCC                GGATG(2/0)
              950  GGATG               
CelII        1003  GCTGAGC              GC^T[ACGT]AGC
             1054  GCTCAGC             
CfoI          870  GCGC                 GCG^C
              885  GCGC                
DraII         179  GGGTCCC              [GA]G^G[ACGT]CC[CT]
              982  GGGGCCC             
EcoO109I      179  GGGTCCC              [GA]G^G[ACGT]CC[CT]
              982  GGGGCCC             
FokI          102  CATCC                GGATG(9/13)
              950  GGATG               
HaeII         869  AGCGCT               [GA]GCGC^[CT]
              884  AGCGCC              
HhaI          870  GCGC                 GCG^C
              885  GCGC                
HinP1I        870  GCGC                 G^CGC
              885  GCGC                
HincII        418  GTCAAC               GT[CT]^[GA]AC
              679  GTCAAC              
HindII        418  GTCAAC               GT[CT]^[GA]AC
              679  GTCAAC              
HinfI        1229  GACTC                G^A[ACGT]TC
             1257  GAATC               
MaeI          889  CTAG                 C^TAG
             1072  CTAG                
MaeIII        193  GTCAC                ^GT[ACGT]AC
             1177  GTGAC               
MboII         270  GAAGA                GAAGA(8/7)
              654  GAAGA               
PvuII        1036  CAGCTG               CAG^CTG
             1057  CAGCTG              
Tsp45I        193  GTCAC                ^GT[GC]AC
             1177  GTGAC               
Tsp509I       377  AATT                 ^AATT
              915  AATT                


#########################################
# 3 OR MORE CUT ENZYMES (   66 enzymes) #
#########################################

AciI        AfaI        AluI        AlwI        AvaI        
AvaII       BalI        BanII       BbvI        BcnI        
BglI        BmyI        BpmI        BsaJI       BseRI       
BsgI        BsiYI       BslI        BsmFI       BsoBI       
Bsp1286I    BspMI       BsrI        BssKI       BstNI       
BstYI       Cac8I       Cfr13I      DdeI        DpnII       
DsaI        EaeI        EcoRII      EcoT14I     FauI        
Fnu4HI      HaeIII      HapII       HpaII       ItaI        
MboI        MflI        MluNI       MnlI        MscI        
MslI        MspA1I      MspI        MvaI        MwoI        
NciI        NcoI        NdeII       NlaIII      NlaIV       
RsaI        Sau3AI      Sau96I      ScrFI       SfaNI       
StyI        TaqI        TseI        TspRI       TthHB8I     
XhoII       
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