Restriction map for pF1KB6681
 (Linear) (Six-base) MAPSORT of: KIBB6681.seq  Check: 6466  from: 1  to: 1281



KIBB6681.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:22  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     753     759     780    1281 
   Size:        753       6      21     501

  Fragments arranged by size: 

                 753     501      21       6
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     472     673     703    1103    1281 
   Size:        472     201      30     400     178

  Fragments arranged by size: 

                 472     400     201     178      30
 
AfeI AGC'GCT

Cuts at:      0     871    1281 
   Size:        871     410
 
AleI CACnn'nnGTG

Cuts at:      0    1138    1237    1281 
   Size:       1138      99      44

  Fragments arranged by size: 

                1138      99      44
 
AlwNI CAG_nnn'CTG

Cuts at:      0     196     609    1281 
   Size:        196     413     672

  Fragments arranged by size: 

                 672     413     196
 
ApaI G_GGCC'C

Cuts at:      0     786     987    1281 
   Size:        786     201     294

  Fragments arranged by size: 

                 786     294     201
 
ApoI r'AATT_y

Cuts at:      0     376    1281 
   Size:        376     905
 
AvaI C'yCGr_G

Cuts at:      0     842     986     992    1281 
   Size:        842     144       6     289

  Fragments arranged by size: 

                 842     289     144       6
 
BamHI G'GATC_C

Cuts at:      0     563    1281 
   Size:        563     718
 
BanII G_rGCy'C

Cuts at:      0     786     987     993    1281 
   Size:        786     201       6     288

  Fragments arranged by size: 

                 786     288     201       6
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     277    1281 
   Size:        277    1004
 
BbvCI CC'TCA_GC

Cuts at:      0     965    1281 
   Size:        965     316
 
BglI GCCn_nnn'nGGC

Cuts at:      0     334     982    1021    1281 
   Size:        334     648      39     260

  Fragments arranged by size: 

                 648     334     260      39
 
BglII A'GATC_T

Cuts at:      0     356    1281 
   Size:        356     925
 
BlpI GC'TnA_GC

Cuts at:      0    1004    1055    1281 
   Size:       1004      51     226

  Fragments arranged by size: 

                1004     226      51
 
Bme1580I G_kGCm'C

Cuts at:      0     786     987    1281 
   Size:        786     201     294

  Fragments arranged by size: 

                 786     294     201
 
BmrI ACTGGGnnnn_n'

Cuts at:      0      55    1190    1281 
   Size:         55    1135      91

  Fragments arranged by size: 

                1135      91      55
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     140    1281 
   Size:        140    1141
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      95     322     856     901    1281 
   Size:         95     227     534      45     380

  Fragments arranged by size: 

                 534     380     227      95      45
 
Bpu10I CC'TnA_GC

Cuts at:      0     965    1281 
   Size:        965     316
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     494    1281 
   Size:        494     787
 
BsaI GGTCTCn'nnnn_

Cuts at:      0      42    1281 
   Size:         42    1239
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0      68      98    1281 
   Size:         68      30    1183

  Fragments arranged by size: 

                1183      68      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     367     396     763    1111    1281 
   Size:        367      29     367     348     170

  Fragments arranged by size: 

                 367     367     348     170      29
 
BseYI C'CCAG_C

Cuts at:      0     559     785    1281 
   Size:        559     226     496

  Fragments arranged by size: 

                 559     496     226
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     781     787     820    1281 
   Size:        781       6      33     461

  Fragments arranged by size: 

                 781     461      33       6
 
BsiEI CG_ry'CG

Cuts at:      0     248    1281 
   Size:        248    1033
 
BsiHKAI G_wGCw'C

Cuts at:      0     696    1281 
   Size:        696     585
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     298    1281 
   Size:        298     983
 
Bsp1286I G_dGCh'C

Cuts at:      0     696     786     987     993    1281 
   Size:        696      90     201       6     288

  Fragments arranged by size: 

                 696     288     201      90       6
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     753     759     780    1281 
   Size:        753       6      21     501

  Fragments arranged by size: 

                 753     501      21       6
 
BsrBI CCG'CTC

Cuts at:      0     780    1281 
   Size:        780     501
 
BsrDI GCAATG_nn'

Cuts at:      0     200     293    1281 
   Size:        200      93     988

  Fragments arranged by size: 

                 988     200      93
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     759    1281 
   Size:        759     522
 
BstYI r'GATC_y

Cuts at:      0     110     119     356     563    1281 
   Size:        110       9     237     207     718

  Fragments arranged by size: 

                 718     237     207     110       9
 
BtgI C'CryG_G

Cuts at:      0      29     174    1160    1281 
   Size:         29     145     986     121

  Fragments arranged by size: 

                 986     145     121      29
 
BtsI GCAGTG_nn'

Cuts at:      0     803    1158    1281 
   Size:        803     355     123

  Fragments arranged by size: 

                 803     355     123
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     676    1281 
   Size:        676     605
 
EaeI y'GGCC_r

Cuts at:      0     245     626     725    1013    1281 
   Size:        245     381      99     288     268

  Fragments arranged by size: 

                 381     288     268     245      99
 
EagI C'GGCC_G

Cuts at:      0     245    1281 
   Size:        245    1036
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     940    1281 
   Size:        940     341
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      95     322     472     673     703     856     901    1103 
   Size:         95     227     150     201      30     153      45     202

Cuts at:   1103    1281 
   Size:        178

  Fragments arranged by size: 

                 227     202     201     178     153     150      95      45
                  30
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     892    1281 
   Size:        892     389
 
EcoO109I rG'GnC_Cy

Cuts at:      0     180     983    1281 
   Size:        180     803     298

  Fragments arranged by size: 

                 803     298     180
 
HaeII r_GCGC'y

Cuts at:      0     873     888    1281 
   Size:        873      15     393

  Fragments arranged by size: 

                 873     393      15
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      68     100    1092    1124    1281 
   Size:         68      32     992      32     157

  Fragments arranged by size: 

                 992     157      68      32      32
 
HincII GTy'rAC

Cuts at:      0     420     681    1281 
   Size:        420     261     600

  Fragments arranged by size: 

                 600     420     261
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     129    1281 
   Size:        129    1152
 
MscI TGG'CCA

Cuts at:      0     628     727    1015    1281 
   Size:        628      99     288     266

  Fragments arranged by size: 

                 628     288     266      99
 
MslI CAynn'nnrTG

Cuts at:      0     346     433    1138    1237    1281 
   Size:        346      87     705      99      44

  Fragments arranged by size: 

                 705     346      99      87      44
 
MspA1I CmG'CkG

Cuts at:      0     756     859    1038    1059    1148    1281 
   Size:        756     103     179      21      89     133

  Fragments arranged by size: 

                 756     179     133     103      89      21
 
NcoI C'CATG_G

Cuts at:      0      29     174    1160    1281 
   Size:         29     145     986     121

  Fragments arranged by size: 

                 986     145     121      29
 
NotI GC'GGCC_GC

Cuts at:      0     245    1281 
   Size:        245    1036
 
NspI r_CATG'y

Cuts at:      0    1068    1281 
   Size:       1068     213
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     792    1281 
   Size:        792     489
 
PfoI T'CCnGG_A

Cuts at:      0     566    1281 
   Size:        566     715
 
PpuMI rG'GwC_Cy

Cuts at:      0     180    1281 
   Size:        180    1101
 
PspOMI G'GGCC_C

Cuts at:      0     782     983    1281 
   Size:        782     201     298

  Fragments arranged by size: 

                 782     298     201
 
PvuII CAG'CTG

Cuts at:      0    1038    1059    1281 
   Size:       1038      21     222

  Fragments arranged by size: 

                1038     222      21
 
SanDI GG'GwC_CC

Cuts at:      0     180    1281 
   Size:        180    1101
 
ScaI AGT'ACT

Cuts at:      0    1190    1281 
   Size:       1190      91
 
SexAI A'CCwGG_T

Cuts at:      0     413    1281 
   Size:        413     868
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0     334    1281 
   Size:        334     947
 
SmaI CCC'GGG

Cuts at:      0     844    1281 
   Size:        844     437
 
SmlI C'TyrA_G

Cuts at:      0     509    1281 
   Size:        509     772
 
StuI AGG'CCT

Cuts at:      0     970    1281 
   Size:        970     311
 
StyI C'CwwG_G

Cuts at:      0      29     174     629    1160    1281 
   Size:         29     145     455     531     121

  Fragments arranged by size: 

                 531     455     145     121      29
 
TatI w'GTAC_w

Cuts at:      0    1188    1281 
   Size:       1188      93
 
Tth111I GACn'n_nGTC

Cuts at:      0     415    1281 
   Size:        415     866
 
XmaI C'CCGG_G

Cuts at:      0     842    1281 
   Size:        842     439
 

 Enzymes that do cut:

     AarI     AcuI     AfeI     AleI    AlwNI     ApaI     ApoI     AvaI
    BamHI    BanII     BbsI    BbvCI     BglI    BglII     BlpI Bme1580I
     BmrI     BplI     BpmI   Bpu10I    BpuEI     BsaI    BsaXI    BseRI
    BseYI     BsgI    BsiEI  BsiHKAI    BsmBI Bsp1286I    BspMI    BsrBI
    BsrDI   BstAPI    BstYI     BtgI     BtsI     DrdI     EaeI     EagI
     EciI  Eco57MI    EcoNI EcoO109I    HaeII    Hin4I   HincII     MmeI
     MscI     MslI   MspA1I     NcoI     NotI     NspI    PflMI     PfoI
    PpuMI   PspOMI    PvuII    SanDI     ScaI    SexAI     SfiI     SmaI
     SmlI     StuI     StyI     TatI  Tth111I     XmaI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI    AflII   AflIII     AgeI     AhdI
     AlfI     AloI    ApaLI     AscI     AseI    AsiSI    AvrII     BaeI
     BanI     BbeI     BcgI    BciVI     BclI    BfrBI    BmgBI     BmtI
    BsaAI    BsaBI    BsaHI    BsaWI    BsiWI     BsmI    BspEI    BspHI
    BsrFI    BsrGI   BssHII    BssSI    BstBI   BstEII    BstXI  BstZ17I
   Bsu36I    BtgZI     ClaI    CspCI     DraI   DraIII     EarI  EcoICRI
    EcoRI    EcoRV     FalI     FseI     FspI    FspAI  HindIII     HpaI
     KasI     KpnI     MfeI     MluI     NaeI     NarI     NdeI   NgoMIV
     NheI     NruI     NsiI     PacI     PasI     PciI     PmeI     PmlI
     PpiI    PshAI     PsiI    PspXI     PsrI     PstI     PvuI    RsrII
     SacI    SacII     SalI     SapI     SbfI     SfcI     SfoI     SgfI
    SgrAI    SnaBI     SpeI     SphI     SrfI     SspI     SwaI    TaqII
     TstI     XbaI     XcmI     XhoI     XmnI     ZraI
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