(Linear) (Six-base) MAPSORT of: KIBB5349.seq Check: 9715 from: 1 to: 1128 KIBB5349.orf With 182 enzymes: SgfI * October 7, 2009 14:16 .. AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 100 772 1128 Size: 100 672 356 Fragments arranged by size: 672 356 100 AflIII A'CryG_T Cuts at: 0 506 1128 Size: 506 622 AleI CACnn'nnGTG Cuts at: 0 1075 1128 Size: 1075 53 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1108 1128 Size: 1108 20 AlwNI CAG_nnn'CTG Cuts at: 0 571 696 750 804 1128 Size: 571 125 54 54 324 Fragments arranged by size: 571 324 125 54 54 ApaI G_GGCC'C Cuts at: 0 47 1128 Size: 47 1081 ApaLI G'TGCA_C Cuts at: 0 1068 1128 Size: 1068 60 ApoI r'AATT_y Cuts at: 0 196 812 1128 Size: 196 616 316 Fragments arranged by size: 616 316 196 AvaI C'yCGr_G Cuts at: 0 46 1013 1128 Size: 46 967 115 Fragments arranged by size: 967 115 46 BamHI G'GATC_C Cuts at: 0 138 1128 Size: 138 990 BanI G'GyrC_C Cuts at: 0 65 1128 Size: 65 1063 BanII G_rGCy'C Cuts at: 0 40 47 83 360 1128 Size: 40 7 36 277 768 Fragments arranged by size: 768 277 40 36 7 BbsI GAAGACnn'nnnn_ Cuts at: 0 760 1128 Size: 760 368 Bme1580I G_kGCm'C Cuts at: 0 47 1072 1128 Size: 47 1025 56 Fragments arranged by size: 1025 56 47 BmtI G_CTAG'C Cuts at: 0 607 1128 Size: 607 521 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 181 664 1128 Size: 181 483 464 Fragments arranged by size: 483 464 181 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 94 459 685 1078 1128 Size: 94 365 226 393 50 Fragments arranged by size: 393 365 226 94 50 BsaI GGTCTCn'nnnn_ Cuts at: 0 169 1128 Size: 169 959 BsaAI yAC'GTr Cuts at: 0 246 1128 Size: 246 882 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 1051 1081 1128 Size: 1051 30 47 Fragments arranged by size: 1051 47 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 91 637 1128 Size: 91 546 491 Fragments arranged by size: 546 491 91 BseYI C'CCAG_C Cuts at: 0 597 797 1128 Size: 597 200 331 Fragments arranged by size: 597 331 200 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 154 1027 1128 Size: 154 873 101 Fragments arranged by size: 873 154 101 BsiHKAI G_wGCw'C Cuts at: 0 40 83 360 1072 1106 1128 Size: 40 43 277 712 34 22 Fragments arranged by size: 712 277 43 40 34 22 Bsp1286I G_dGCh'C Cuts at: 0 40 47 83 360 1072 1106 1128 Size: 40 7 36 277 712 34 22 Fragments arranged by size: 712 277 40 36 34 22 7 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 861 1128 Size: 861 267 BsrBI CCG'CTC Cuts at: 0 738 1128 Size: 738 390 BsrFI r'CCGG_y Cuts at: 0 686 1128 Size: 686 442 BssSI C'ACGA_G Cuts at: 0 226 299 1128 Size: 226 73 829 Fragments arranged by size: 829 226 73 BstAPI GCAn_nnn'nTGC Cuts at: 0 660 1128 Size: 660 468 BstXI CCAn_nnnn'nTGG Cuts at: 0 160 1128 Size: 160 968 BstYI r'GATC_y Cuts at: 0 138 558 1128 Size: 138 420 570 Fragments arranged by size: 570 420 138 Bsu36I CC'TnA_GG Cuts at: 0 673 1128 Size: 673 455 BtsI GCAGTG_nn' Cuts at: 0 335 697 1128 Size: 335 362 431 Fragments arranged by size: 431 362 335 DraIII CAC_nnn'GTG Cuts at: 0 210 660 1128 Size: 210 450 468 Fragments arranged by size: 468 450 210 DrdI GACnn_nn'nnGTC Cuts at: 0 988 1128 Size: 988 140 EarI CTCTTCn'nnn_ Cuts at: 0 1075 1128 Size: 1075 53 EcoICRI GAG'CTC Cuts at: 0 38 81 358 1128 Size: 38 43 277 770 Fragments arranged by size: 770 277 43 38 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 94 100 459 685 772 1078 1128 Size: 94 6 359 226 87 306 50 Fragments arranged by size: 359 306 226 94 87 50 6 EcoO109I rG'GnC_Cy Cuts at: 0 378 939 1128 Size: 378 561 189 Fragments arranged by size: 561 378 189 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 817 1128 Size: 817 311 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 249 281 1128 Size: 249 32 847 Fragments arranged by size: 847 249 32 HincII GTy'rAC Cuts at: 0 549 1128 Size: 549 579 HindIII A'AGCT_T Cuts at: 0 172 1128 Size: 172 956 MfeI C'AATT_G Cuts at: 0 350 401 1128 Size: 350 51 727 Fragments arranged by size: 727 350 51 MluI A'CGCG_T Cuts at: 0 506 1128 Size: 506 622 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 1001 1128 Size: 1001 127 MslI CAynn'nnrTG Cuts at: 0 835 1075 1090 1128 Size: 835 240 15 38 Fragments arranged by size: 835 240 38 15 MspA1I CmG'CkG Cuts at: 0 58 108 1128 Size: 58 50 1020 Fragments arranged by size: 1020 58 50 NheI G'CTAG_C Cuts at: 0 603 1128 Size: 603 525 PpuMI rG'GwC_Cy Cuts at: 0 378 1128 Size: 378 750 PspOMI G'GGCC_C Cuts at: 0 43 1128 Size: 43 1085 PvuII CAG'CTG Cuts at: 0 108 1128 Size: 108 1020 SacI G_AGCT'C Cuts at: 0 40 83 360 1128 Size: 40 43 277 768 Fragments arranged by size: 768 277 43 40 SapI GCTCTTCn'nnn_ Cuts at: 0 1075 1128 Size: 1075 53 SfcI C'TryA_G Cuts at: 0 22 382 1128 Size: 22 360 746 Fragments arranged by size: 746 360 22 SmaI CCC'GGG Cuts at: 0 48 1128 Size: 48 1080 SrfI GCCC'GGGC Cuts at: 0 48 1128 Size: 48 1080 StyI C'CwwG_G Cuts at: 0 153 1128 Size: 153 975 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 993 1128 Size: 993 135 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1051 1083 1128 Size: 1051 32 45 Fragments arranged by size: 1051 45 32 XmaI C'CCGG_G Cuts at: 0 46 1128 Size: 46 1082 XmnI GAAnn'nnTTC Cuts at: 0 236 1128 Size: 236 892 Enzymes that do cut: AcuI AflIII AleI AlfI AlwNI ApaI ApaLI ApoI AvaI BamHI BanI BanII BbsI Bme1580I BmtI BplI BpmI BsaI BsaAI BsaXI BseRI BseYI BsgI BsiHKAI Bsp1286I BspMI BsrBI BsrFI BssSI BstAPI BstXI BstYI Bsu36I BtsI DraIII DrdI EarI EcoICRI Eco57MI EcoO109I FalI Hin4I HincII HindIII MfeI MluI MmeI MslI MspA1I NheI PpuMI PspOMI PvuII SacI SapI SfcI SmaI SrfI StyI TaqII TstI XmaI XmnI Enzymes that do not cut: AarI AatII AccI Acc65I AclI AfeI AflII AgeI AhdI AloI AscI AseI AsiSI AvrII BaeI BbeI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI BmgBI BmrI Bpu10I BpuEI BsaBI BsaHI BsaWI BsiEI BsiWI BsmI BsmBI BspEI BspHI BsrDI BsrGI BssHII BstBI BstEII BstZ17I BtgI BtgZI ClaI CspCI DraI EaeI EagI EciI EcoNI EcoRI EcoRV FseI FspI FspAI HaeII HpaI KasI KpnI MscI NaeI NarI NcoI NdeI NgoMIV NotI NruI NsiI NspI PacI PasI PciI PflMI PfoI PmeI PmlI PpiI PshAI PsiI PspXI PsrI PstI PvuI RsrII SacII SalI SanDI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmlI SnaBI SpeI SphI SspI StuI SwaI TatI Tth111I XbaI XcmI XhoI ZraI