Restriction map for pF1KB0980
##################################
# NO CUT ENZYMES (  127 enzymes) #
##################################

AatII       Acc65I      AclI        AcsI        AflII       
AflIII      AgeI        AhdI        AlwNI       AocI        
Aor51HI     ApoI        AscI        Asp700I     Asp718I     
AspEI       AspI        AviII       AvrII       BamHI       
BanI        BbeI        BbrPI       BbsI        BcgI        
BfrI        BlnI        BpuAI       BsaAI       BsaBI       
BsiEI       BsiWI       BsmBI       BsmI        Bsp1407I    
BspDI       BspHI       BspLU11I    BsrGI       BssHII      
BssSI       Bst1107I    BstAPI      BstBI       BstEII      
BstPI       BstZ17I     Bsu36I      ClaI        CpoI        
DraI        DraIII      DrdI        EagI        Eam1105I    
EarI        EclXI       Eco47III    Eco52I      Eco81I      
EcoO65I     EcoRI       EcoRV       EcoT22I     FseI        
FspI        HincII      HindII      HindIII     HpaI        
KasI        KpnI        Ksp632I     KspI        MaeII       
MamI        MfeI        MluI        MunI        NaeI        
NarI        NdeI        NgoMIV      NheI        NotI        
NruI        NsiI        NspI        NspV        PacI        
PflFI       PflMI       PinAI       PmaCI       PmeI        
PmlI        PshAI       Psp1406I    PvuI        RcaI        
RsrII       SacI        SacII       SalI        SapI        
SbfI        ScaI        SexAI       SfiI        SfoI        
SfuI        SgrAI       SmaI        SnaBI       SpeI        
SphI        Sse8387I    SspBI       SspI        SwaI        
Tsp509I     Tth111I     Van91I      XbaI        XmaCI       
XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   41 enzymes) #
###################################

AccI          103  GTAGAC               GT^[AC][GT]AC
AccII         154  CGCG                 CG^CG
AccIII        395  TCCGGA               T^CCGGA
AcyI          343  GGCGTC               G[GA]^CG[CT]C
Alw44I       1313  GTGCAC               G^TGCAC
ApaI         1102  GGGCCC               GGGCC^C
ApaLI        1313  GTGCAC               G^TGCAC
AseI          382  ATTAAT               AT^TAAT
AsnI          382  ATTAAT               AT^TAAT
AspHI        1313  GTGCAC               G[AT]GC[AT]^C
BbvCI         337  GCTGAGG              CCTCAGC(-5/-2)
BciVI        1117  GGATAC               GTATCC(6/5)
BclI          603  TGATCA               T^GATCA
BglI          849  GCCACTGGGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII          19  AGATCT               A^GATCT
BsaHI         343  GGCGTC               G[GA]^CG[CT]C
BsaI          323  GAGACC               GGTCTC(1/5)
BsaWI         395  TCCGGA               [AT]^CCGG[AT]
BseAI         395  TCCGGA               T^CCGGA
BsgI          406  GTGCAG               GTGCAG(16/14)
BsiHKAI      1313  GTGCAC               G[AT]GC[AT]^C
BspEI         395  TCCGGA               T^CCGGA
BspMI         188  GCAGGT               ACCTGC(4/8)
BsrDI        1205  GCAATG               GCAATG(2/0)
BsrFI         326  ACCGGC               [GA]^CCGG[CT]
BstUI         154  CGCG                 CG^CG
BstXI         257  CCAAGGTGGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr10I        326  ACCGGC               [GA]^CCGG[CT]
EcoNI         333  CCTGGCTGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FbaI          603  TGATCA               T^GATCA
HaeII        1250  GGCGCT               [GA]GCGC^[CT]
Hin1I         343  GGCGTC               G[GA]^CG[CT]C
MboII         270  GAAGA                GAAGA(8/7)
MroI          395  TCCGGA               T^CCGGA
MseI          383  TTAA                 T^TAA
MslI          115  CACCAGGGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI          154  CGCG                 CG^CG
PpuMI        1013  GGGTCCC              [GA]G^G[AT]CC[CT]
PshBI         382  ATTAAT               AT^TAAT
PstI          994  CTGCAG               CTGCA^G
Tru9I         383  TTAA                 T^TAA


###################################
# TWO CUT ENZYMES (   19 enzymes) #
###################################

AfaI          192  GTAC                 GT^AC
             1053  GTAC                
BfaI          950  CTAG                 C^TAG
              974  CTAG                
BlpI          485  GCTCAGC              GC^T[ACGT]AGC
              565  GCTCAGC             
BpmI         1263  CTCCAG               CTGGAG(16/14)
              218  CTGGAG              
Bpu1102I      485  GCTCAGC              GC^T[ACGT]AGC
              565  GCTCAGC             
BsmAI         199  GAGAC                GTCTC(1/5)
              323  GAGAC               
BsrBI         703  CCGCTC               CCGCTC(-3/-3)
              721  GAGCGG              
CelII         485  GCTCAGC              GC^T[ACGT]AGC
              565  GCTCAGC             
FauI          786  CCCGC                CCCGC(4/6)
             1017  CCCGC               
MaeI          950  CTAG                 C^TAG
              974  CTAG                
NcoI           27  CCATGG               C^CATGG
              162  CCATGG              
PaeR7I        697  CTCGAG               C^TCGAG
              706  CTCGAG              
RsaI          192  GTAC                 GT^AC
             1053  GTAC                
SfcI          994  CTGCAG               C^T[GA][CT]AG
             1236  CTACAG              
SmlI          697  CTCGAG               C^T[CT][GA]AG
              706  CTCGAG              
StuI          740  AGGCCT               AGG^CCT
             1244  AGGCCT              
TfiI          389  GAATC                G^A[AT]TC
              520  GATTC               
Tsp45I        350  GTGAC                ^GT[GC]AC
              507  GTCAC               
XhoI          697  CTCGAG               C^TCGAG
              706  CTCGAG              


#########################################
# 3 OR MORE CUT ENZYMES (   71 enzymes) #
#########################################

AciI        AluI        AlwI        AvaI        AvaII       
BalI        BanII       BbvI        BcnI        BmyI        
BsaJI       BseRI       BsiYI       BslI        BsmFI       
BsoBI       Bsp1286I    BsrI        BssKI       BstF5I      
BstNI       BstYI       Cac8I       CfoI        Cfr13I      
DdeI        DpnII       DraII       DsaI        EaeI        
EcoO109I    EcoRII      EcoT14I     Fnu4HI      FokI        
HaeIII      HapII       HgaI        HhaI        HinP1I      
HinfI       HpaII       HphI        ItaI        MaeIII      
MboI        MflI        MluNI       MnlI        MscI        
MspA1I      MspI        MvaI        MwoI        NciI        
NdeII       NlaIII      NlaIV       PleI        PvuII       
Sau3AI      Sau96I      ScrFI       SfaNI       StyI        
TaqI        TseI        TspRI       TthHB8I     XcmI        
XhoII       
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com