Restriction map for pF1KB0980
 (Linear) (Six-base) MAPSORT of: KIBB0980.seq  Check: 1587  from: 1  to: 1350



KIBB0980.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:15  ..

AccI GT'mk_AC

Cuts at:      0     104    1350 
   Size:        104    1246
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     666    1350 
   Size:        666     684
 
AleI CACnn'nnGTG

Cuts at:      0     119    1350 
   Size:        119    1231
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0      91    1350 
   Size:         91    1259
 
ApaI G_GGCC'C

Cuts at:      0    1106    1350 
   Size:       1106     244
 
ApaLI G'TGCA_C

Cuts at:      0    1313    1350 
   Size:       1313      37
 
AseI AT'TA_AT

Cuts at:      0     383    1350 
   Size:        383     967
 
AvaI C'yCGr_G

Cuts at:      0     697     706     934    1350 
   Size:        697       9     228     416

  Fragments arranged by size: 

                 697     416     228       9
 
BanII G_rGCy'C

Cuts at:      0     235     532     554    1106    1350 
   Size:        235     297      22     552     244

  Fragments arranged by size: 

                 552     297     244     235      22
 
BbvCI CC'TCA_GC

Cuts at:      0     338    1350 
   Size:        338    1012
 
BciVI GTATCCnnnnn_n'

Cuts at:      0    1111    1350 
   Size:       1111     239
 
BclI T'GATC_A

Cuts at:      0     603    1350 
   Size:        603     747
 
BglI GCCn_nnn'nGGC

Cuts at:      0     855    1350 
   Size:        855     495
 
BglII A'GATC_T

Cuts at:      0      19    1350 
   Size:         19    1331
 
BlpI GC'TnA_GC

Cuts at:      0     486     566    1350 
   Size:        486      80     784

  Fragments arranged by size: 

                 784     486      80
 
Bme1580I G_kGCm'C

Cuts at:      0     368     715    1106    1317    1350 
   Size:        368     347     391     211      33

  Fragments arranged by size: 

                 391     368     347     211      33
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     362     507     862    1350 
   Size:        362     145     355     488

  Fragments arranged by size: 

                 488     362     355     145
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     346    1350 
   Size:        346    1004
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     239    1248    1350 
   Size:        239    1009     102

  Fragments arranged by size: 

                1009     239     102
 
Bpu10I CC'TnA_GC

Cuts at:      0     338    1350 
   Size:        338    1012
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     317    1350 
   Size:        317    1033
 
BsaHI Gr'CG_yC

Cuts at:      0     344    1350 
   Size:        344    1006
 
BsaWI w'CCGG_w

Cuts at:      0     395    1350 
   Size:        395     955
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0    1069    1152    1221    1350 
   Size:       1069      83      69     129

  Fragments arranged by size: 

                1069     129      83      69
 
BseYI C'CCAG_C

Cuts at:      0      45     481     754    1350 
   Size:         45     436     273     596

  Fragments arranged by size: 

                 596     436     273      45
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     427    1350 
   Size:        427     923
 
BsiHKAI G_wGCw'C

Cuts at:      0    1317    1350 
   Size:       1317      33
 
Bsp1286I G_dGCh'C

Cuts at:      0     235     368     532     554     715    1106    1317    1350 
   Size:        235     133     164      22     161     391     211      33

  Fragments arranged by size: 

                 391     235     211     164     161     133      33      22
            
 
BspEI T'CCGG_A

Cuts at:      0     395    1350 
   Size:        395     955
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     179    1350 
   Size:        179    1171
 
BsrBI CCG'CTC

Cuts at:      0     705     723    1350 
   Size:        705      18     627

  Fragments arranged by size: 

                 705     627      18
 
BsrDI GCAATG_nn'

Cuts at:      0    1212    1350 
   Size:       1212     138
 
BsrFI r'CCGG_y

Cuts at:      0     326    1350 
   Size:        326    1024
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     264    1350 
   Size:        264    1086
 
BstYI r'GATC_y

Cuts at:      0      19     179     317     897     990    1350 
   Size:         19     160     138     580      93     360

  Fragments arranged by size: 

                 580     360     160     138      93      19
 
BtgI C'CryG_G

Cuts at:      0      27     162     887    1331    1350 
   Size:         27     135     725     444      19

  Fragments arranged by size: 

                 725     444     135      27      19
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     546    1155    1350 
   Size:        546     609     195

  Fragments arranged by size: 

                 609     546     195
 
BtsI GCAGTG_nn'

Cuts at:      0    1325    1350 
   Size:       1325      25
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0    1320    1350 
   Size:       1320      30
 
EaeI y'GGCC_r

Cuts at:      0      24     248     443    1215    1350 
   Size:         24     224     195     772     135

  Fragments arranged by size: 

                 772     224     195     135      24
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0      79     969    1350 
   Size:         79     890     381

  Fragments arranged by size: 

                 890     381      79
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     239     666    1248    1350 
   Size:        239     427     582     102

  Fragments arranged by size: 

                 582     427     239     102
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     337    1350 
   Size:        337    1013
 
EcoO109I rG'GnC_Cy

Cuts at:      0      53     128     777    1014    1074    1102    1350 
   Size:         53      75     649     237      60      28     248

  Fragments arranged by size: 

                 649     248     237      75      60      53      28
 
HaeII r_GCGC'y

Cuts at:      0    1254    1350 
   Size:       1254      96
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     889     921    1350 
   Size:        889      32     429

  Fragments arranged by size: 

                 889     429      32
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     497     866    1350 
   Size:        497     369     484

  Fragments arranged by size: 

                 497     484     369
 
MscI TGG'CCA

Cuts at:      0      26     250     445    1217    1350 
   Size:         26     224     195     772     133

  Fragments arranged by size: 

                 772     224     195     133      26
 
MslI CAynn'nnrTG

Cuts at:      0     119    1350 
   Size:        119    1231
 
MspA1I CmG'CkG

Cuts at:      0      45     437     490     650     789    1142    1350 
   Size:         45     392      53     160     139     353     208

  Fragments arranged by size: 

                 392     353     208     160     139      53      45
 
NcoI C'CATG_G

Cuts at:      0      27     162    1350 
   Size:         27     135    1188

  Fragments arranged by size: 

                1188     135      27
 
PasI CC'CwG_GG

Cuts at:      0     525    1350 
   Size:        525     825
 
PfoI T'CCnGG_A

Cuts at:      0     462    1350 
   Size:        462     888
 
PpuMI rG'GwC_Cy

Cuts at:      0    1014    1350 
   Size:       1014     336
 
PspOMI G'GGCC_C

Cuts at:      0    1102    1350 
   Size:       1102     248
 
PspXI vC'TCGA_Gb

Cuts at:      0     697     706    1350 
   Size:        697       9     644

  Fragments arranged by size: 

                 697     644       9
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0    1045    1077    1350 
   Size:       1045      32     273

  Fragments arranged by size: 

                1045     273      32
 
PstI C_TGCA'G

Cuts at:      0     998    1350 
   Size:        998     352
 
PvuII CAG'CTG

Cuts at:      0      45     437     490    1350 
   Size:         45     392      53     860

  Fragments arranged by size: 

                 860     392      53      45
 
SanDI GG'GwC_CC

Cuts at:      0    1014    1350 
   Size:       1014     336
 
SfcI C'TryA_G

Cuts at:      0     994    1236    1350 
   Size:        994     242     114

  Fragments arranged by size: 

                 994     242     114
 
SmlI C'TyrA_G

Cuts at:      0     697     706    1350 
   Size:        697       9     644

  Fragments arranged by size: 

                 697     644       9
 
StuI AGG'CCT

Cuts at:      0     742    1246    1350 
   Size:        742     504     104

  Fragments arranged by size: 

                 742     504     104
 
StyI C'CwwG_G

Cuts at:      0      27     162     183     242     257    1350 
   Size:         27     135      21      59      15    1093

  Fragments arranged by size: 

                1093     135      59      27      21      15
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1115    1147    1350 
   Size:       1115      32     203

  Fragments arranged by size: 

                1115     203      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     243     258     849    1176    1350 
   Size:        243      15     591     327     174

  Fragments arranged by size: 

                 591     327     243     174      15
 
XhoI C'TCGA_G

Cuts at:      0     697     706    1350 
   Size:        697       9     644

  Fragments arranged by size: 

                 697     644       9
 

 Enzymes that do cut:

     AccI     AcuI     AleI     AlfI     ApaI    ApaLI     AseI     AvaI
    BanII    BbvCI    BciVI     BclI     BglI    BglII     BlpI Bme1580I
     BmrI     BplI     BpmI   Bpu10I     BsaI    BsaHI    BsaWI    BseRI
    BseYI     BsgI  BsiHKAI Bsp1286I    BspEI    BspMI    BsrBI    BsrDI
    BsrFI    BstXI    BstYI     BtgI    BtgZI     BtsI    CspCI     EaeI
     EciI  Eco57MI    EcoNI EcoO109I    HaeII    Hin4I     MmeI     MscI
     MslI   MspA1I     NcoI     PasI     PfoI    PpuMI   PspOMI    PspXI
     PsrI     PstI    PvuII    SanDI     SfcI     SmlI     StuI     StyI
     TstI     XcmI     XhoI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AfeI    AflII   AflIII     AgeI
     AhdI     AloI    AlwNI     ApoI     AscI    AsiSI    AvrII     BaeI
    BamHI     BanI     BbeI     BbsI     BcgI    BfrBI    BmgBI     BmtI
    BpuEI    BsaAI    BsaBI    BsaXI    BsiEI    BsiWI     BsmI    BsmBI
    BspHI    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII  BstZ17I
   Bsu36I     ClaI     DraI   DraIII     DrdI     EagI     EarI  EcoICRI
    EcoRI    EcoRV     FalI     FseI     FspI    FspAI   HincII  HindIII
     HpaI     KasI     KpnI     MfeI     MluI     NaeI     NarI     NdeI
   NgoMIV     NheI     NotI     NruI     NsiI     NspI     PacI     PciI
    PflMI     PmeI     PmlI     PpiI    PshAI     PsiI     PvuI    RsrII
     SacI    SacII     SalI     SapI     SbfI     ScaI    SexAI     SfiI
     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI
     SspI     SwaI    TaqII     TatI  Tth111I     XbaI     XmaI     XmnI
     ZraI
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