Restriction map for
pF1KB3606
(Circular) (Six-base) MAPSORT of: pF1KB3606.seq Check: 593 from: 1 to: 3707
pF1KB3606 3707 bp
With 182 enzymes: SgfI *
April 24, 2009 13:02 ..
AccI GT'mk_AC
Cuts at: 745 745
Size: 3707
Acc65I G'GTAC_C
Cuts at: 723 723
Size: 3707
AclI AA'CG_TT
Cuts at: 3547 3547
Size: 3707
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1532 1964 2711 2979 1532
Size: 432 747 268 2260
Fragments arranged by size:
2260 747 432 268
AflIII A'CryG_T
Cuts at: 1059 2163 3140 1059
Size: 1104 977 1626
Fragments arranged by size:
1626 1104 977
AgeI A'CCGG_T
Cuts at: 2095 2095
Size: 3707
AleI CACnn'nnGTG
Cuts at: 163 253 163
Size: 90 3617
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 331 363 331
Size: 32 3675
AlwNI CAG_nnn'CTG
Cuts at: 145 1012 2579 145
Size: 867 1567 1273
Fragments arranged by size:
1567 1273 867
ApaI G_GGCC'C
Cuts at: 400 400
Size: 3707
ApaLI G'TGCA_C
Cuts at: 2477 2477
Size: 3707
ApoI r'AATT_y
Cuts at: 711 3060 3316 711
Size: 2349 256 1102
Fragments arranged by size:
2349 1102 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3707
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3707
AvaI C'yCGr_G
Cuts at: 727 727
Size: 3707
BamHI G'GATC_C
Cuts at: 732 732
Size: 3707
BanI G'GyrC_C
Cuts at: 175 723 1137 1385 1420 175
Size: 548 414 248 35 2462
Fragments arranged by size:
2462 548 414 248 35
BanII G_rGCy'C
Cuts at: 400 721 1751 400
Size: 321 1030 2356
Fragments arranged by size:
2356 1030 321
BbeI G_GCGC'C
Cuts at: 179 1141 1389 179
Size: 962 248 2497
Fragments arranged by size:
2497 962 248
BciVI GTATCCnnnnn_n'
Cuts at: 1605 2366 3707 1605
Size: 761 1341 1605
Fragments arranged by size:
1605 1341 761
BclI T'GATC_A
Cuts at: 1227 1227
Size: 3707
BglII A'GATC_T
Cuts at: 1222 3259 1222
Size: 2037 1670
BlpI GC'TnA_GC
Cuts at: 819 819
Size: 3707
Bme1580I G_kGCm'C
Cuts at: 262 400 1332 1425 2481 262
Size: 138 932 93 1056 1488
Fragments arranged by size:
1488 1056 932 138 93
BmgBI CAC'GTC
Cuts at: 426 426
Size: 3707
BmrI ACTGGGnnnn_n'
Cuts at: 919 1000 1335 3479 919
Size: 81 335 2144 1147
Fragments arranged by size:
2144 1147 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2074 2074
Size: 3707
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 883 1762 2254 2552 883
Size: 879 492 298 2038
Fragments arranged by size:
2038 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3411 3411
Size: 3707
BsaAI yAC'GTr
Cuts at: 219 1060 219
Size: 841 2866
BsaBI GATnn'nnATC
Cuts at: 6 1221 3258 6
Size: 1215 2037 455
Fragments arranged by size:
2037 1215 455
BsaHI Gr'CG_yC
Cuts at: 176 329 1138 1386 2088 3584 176
Size: 153 809 248 702 1496 299
Fragments arranged by size:
1496 809 702 299 248 153
BsaWI w'CCGG_w
Cuts at: 115 896 1417 2095 2369 2516 2927 115
Size: 781 521 678 274 147 411 895
Fragments arranged by size:
895 781 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 309 324 1132 1890 2467 2963 309
Size: 15 808 758 577 496 1053
Fragments arranged by size:
1053 808 758 577 496 15
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 311 311
Size: 3707
BsiEI CG_ry'CG
Cuts at: 104 1249 1295 2503 104
Size: 1145 46 1208 1308
Fragments arranged by size:
1308 1208 1145 46
BsiHKAI G_wGCw'C
Cuts at: 721 1499 1689 2481 721
Size: 778 190 792 1947
Fragments arranged by size:
1947 792 778 190
BsmBI CGTCTCn'nnnn_
Cuts at: 336 336
Size: 3707
Bsp1286I G_dGCh'C
Cuts at: 262 400 721 1332 1425 1499 1689 1751 2481
Size: 138 321 611 93 74 190 62 730
Cuts at: 2481 262
Size: 1488
Fragments arranged by size:
1488 730 611 321 190 138 93 74
62
BspEI T'CCGG_A
Cuts at: 2927 2927
Size: 3707
BspHI T'CATG_A
Cuts at: 2883 2883
Size: 3707
BspMI ACCTGCnnnn'nnnn_
Cuts at: 757 971 1273 1654 757
Size: 214 302 381 2810
Fragments arranged by size:
2810 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1999 2053 3537 3702 50
Size: 1949 54 1484 165 55
Fragments arranged by size:
1949 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 476 1619 476
Size: 1143 2564
BsrFI r'CCGG_y
Cuts at: 1705 1886 2095 1705
Size: 181 209 3317
Fragments arranged by size:
3317 209 181
BssHII G'CGCG_C
Cuts at: 124 124
Size: 3707
BssSI C'ACGA_G
Cuts at: 1978 2336 1978
Size: 358 3349
BstAPI GCAn_nnn'nTGC
Cuts at: 1084 1084
Size: 3707
BstBI TT'CG_AA
Cuts at: 2068 2068
Size: 3707
BstXI CCAn_nnnn'nTGG
Cuts at: 165 165
Size: 3707
BstYI r'GATC_y
Cuts at: 1 732 1199 1222 1557 1803 2815 2901 2913
Size: 731 467 23 335 246 1012 86 12
Cuts at: 2913 3259 1
Size: 346 449
Fragments arranged by size:
1012 731 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 560 107
Size: 453 3254
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 258 1657 1838 2001 258
Size: 1399 181 163 1964
Fragments arranged by size:
1964 1399 181 163
BtsI GCAGTG_nn'
Cuts at: 651 950 651
Size: 299 3408
ClaI AT'CG_AT
Cuts at: 503 503
Size: 3707
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 343 378 343
Size: 35 3672
DraI TTT'AAA
Cuts at: 706 706
Size: 3707
DrdI GACnn_nn'nnGTC
Cuts at: 1413 2271 1413
Size: 858 2849
EaeI y'GGCC_r
Cuts at: 1292 1466 1857 1884 3229 1292
Size: 174 391 27 1345 1770
Fragments arranged by size:
1770 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1292 1292
Size: 3707
EarI CTCTTCn'nnn_
Cuts at: 1730 1940 1730
Size: 210 3497
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1915 2225 2371 3517 1915
Size: 310 146 1146 2105
Fragments arranged by size:
2105 1146 310 146
EcoICRI GAG'CTC
Cuts at: 719 719
Size: 3707
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1532 1964 2711 2979 1532
Size: 432 747 268 2260
Fragments arranged by size:
2260 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 397 489 846 397
Size: 92 357 3258
Fragments arranged by size:
3258 357 92
EcoRI G'AATT_C
Cuts at: 711 711
Size: 3707
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1726 1726
Size: 3707
FspI TGC'GCA
Cuts at: 1488 1488
Size: 3707
HaeII r_GCGC'y
Cuts at: 179 1141 1389 2411 3390 179
Size: 962 248 1022 979 496
Fragments arranged by size:
1022 979 962 496 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1549 1579 1581 1611 1549
Size: 30 2 30 3645
Fragments arranged by size:
3645 30 30 2
HincII GTy'rAC
Cuts at: 746 746
Size: 3707
KasI G'GCGC_C
Cuts at: 175 1137 1385 175
Size: 962 248 2497
Fragments arranged by size:
2497 962 248
KpnI G_GTAC'C
Cuts at: 727 727
Size: 3707
MluI A'CGCG_T
Cuts at: 3140 3140
Size: 3707
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 179 2378 2562 179
Size: 2199 184 1324
Fragments arranged by size:
2199 1324 184
MscI TGG'CCA
Cuts at: 1468 1468
Size: 3707
MslI CAynn'nnrTG
Cuts at: 163 253 1696 1823 163
Size: 90 1443 127 2047
Fragments arranged by size:
2047 1443 127 90
MspA1I CmG'CkG
Cuts at: 163 628 818 1132 1492 2505 2750 163
Size: 465 190 314 360 1013 245 1120
Fragments arranged by size:
1120 1013 465 360 314 245 190
NaeI GCC'GGC
Cuts at: 1888 1888
Size: 3707
NarI GG'CG_CC
Cuts at: 176 1138 1386 176
Size: 962 248 2497
Fragments arranged by size:
2497 962 248
NcoI C'CATG_G
Cuts at: 107 107
Size: 3707
NgoMIV G'CCGG_C
Cuts at: 1886 1886
Size: 3707
NspI r_CATG'y
Cuts at: 760 1260 2167 760
Size: 500 907 2300
Fragments arranged by size:
2300 907 500
PciI A'CATG_T
Cuts at: 2163 2163
Size: 3707
PmeI GTTT'AAAC
Cuts at: 706 706
Size: 3707
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2846 2878 2846
Size: 32 3675
PshAI GACnn'nnGTC
Cuts at: 438 438
Size: 3707
PspOMI G'GGCC_C
Cuts at: 396 396
Size: 3707
PstI C_TGCA'G
Cuts at: 754 1439 754
Size: 685 3022
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3707
PvuII CAG'CTG
Cuts at: 1132 1492 1132
Size: 360 3347
RsrII CG'GwC_CG
Cuts at: 1902 1902
Size: 3707
SacI G_AGCT'C
Cuts at: 721 721
Size: 3707
SalI G'TCGA_C
Cuts at: 744 744
Size: 3707
SapI GCTCTTCn'nnn_
Cuts at: 1730 1940 1730
Size: 210 3497
SbfI CC_TGCA'GG
Cuts at: 754 754
Size: 3707
ScaI AGT'ACT
Cuts at: 3224 3224
Size: 3707
SfcI C'TryA_G
Cuts at: 33 483 750 1435 2428 2619 33
Size: 450 267 685 993 191 1121
Fragments arranged by size:
1121 993 685 450 267 191
SfoI GGC'GCC
Cuts at: 177 1139 1387 177
Size: 962 248 2497
Fragments arranged by size:
2497 962 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3707
SmaI CCC'GGG
Cuts at: 729 729
Size: 3707
SmlI C'TyrA_G
Cuts at: 862 1777 2269 2531 862
Size: 915 492 262 2038
Fragments arranged by size:
2038 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3707
SphI G_CATG'C
Cuts at: 760 1260 760
Size: 500 3207
SspI AAT'ATT
Cuts at: 685 685
Size: 3707
StuI AGG'CCT
Cuts at: 589 589
Size: 3707
StyI C'CwwG_G
Cuts at: 107 841 1195 107
Size: 734 354 2619
Fragments arranged by size:
2619 734 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 367 1290 1423 1882 2091 367
Size: 923 133 459 209 1983
Fragments arranged by size:
1983 923 459 209 133
TatI w'GTAC_w
Cuts at: 596 3222 596
Size: 2626 1081
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 545 577 3224 3256 545
Size: 32 2647 32 996
Fragments arranged by size:
2647 996 32 32
Tth111I GACn'n_nGTC
Cuts at: 1504 1504
Size: 3707
XbaI T'CTAG_A
Cuts at: 738 738
Size: 3707
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1202 1202
Size: 3707
XmaI C'CCGG_G
Cuts at: 727 727
Size: 3707
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AloI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BciVI BclI BglII BlpI Bme1580I
BmgBI BmrI BplI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssHII BssSI BstAPI
BstBI BstXI BstYI BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NspI PciI PmeI PpiI PshAI PspOMI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AlfI AscI AvrII
BaeI BbsI BbvCI BcgI BfrBI BglI BmtI BpmI
Bpu10I BsaXI BseRI BsiWI BsmI BsrGI BstEII BstZ17I
Bsu36I DraIII EcoNI EcoRV FseI FspAI HindIII HpaI
MfeI NdeI NheI NotI NruI NsiI PacI PasI
PflMI PfoI PmlI PpuMI PsiI PspXI PsrI SacII
SanDI SexAI SfiI SgrAI SnaBI SrfI SwaI XhoI
XmnI ZraI