Restriction map for pF1KB6939
 (Linear) (Six-base) MAPSORT of: KIBB6939.seq  Check: 8004  from: 1  to: 624



KIBB6939.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:23  ..

AatII G_ACGT'C

Cuts at:      0     421     624 
   Size:        421     203
 
AfeI AGC'GCT

Cuts at:      0     100     463     624 
   Size:        100     363     161

  Fragments arranged by size: 

                 363     161     100
 
AflIII A'CryG_T

Cuts at:      0     371     624 
   Size:        371     253
 
AgeI A'CCGG_T

Cuts at:      0     568     624 
   Size:        568      56
 
ApaI G_GGCC'C

Cuts at:      0     329     624 
   Size:        329     295
 
AvaI C'yCGr_G

Cuts at:      0     121     287     624 
   Size:        121     166     337

  Fragments arranged by size: 

                 337     166     121
 
BanI G'GyrC_C

Cuts at:      0     437     624 
   Size:        437     187
 
BanII G_rGCy'C

Cuts at:      0      58     220     329     368     624 
   Size:         58     162     109      39     256

  Fragments arranged by size: 

                 256     162     109      58      39
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     478     624 
   Size:        478     146
 
Bme1580I G_kGCm'C

Cuts at:      0     329     379     494     624 
   Size:        329      50     115     130

  Fragments arranged by size: 

                 329     130     115      50
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     324     624 
   Size:        324     300
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      73     624 
   Size:         73     551
 
BsaAI yAC'GTr

Cuts at:      0     374     624 
   Size:        374     250
 
BsaHI Gr'CG_yC

Cuts at:      0     118     418     624 
   Size:        118     300     206

  Fragments arranged by size: 

                 300     206     118
 
BsaWI w'CCGG_w

Cuts at:      0     568     624 
   Size:        568      56
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     310     624 
   Size:        310     314
 
BseYI C'CCAG_C

Cuts at:      0     328     624 
   Size:        328     296
 
BsiHKAI G_wGCw'C

Cuts at:      0      58     503     624 
   Size:         58     445     121

  Fragments arranged by size: 

                 445     121      58
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     425     624 
   Size:        425     199
 
Bsp1286I G_dGCh'C

Cuts at:      0      58     220     329     368     379     494     503     624 
   Size:         58     162     109      39      11     115       9     121

  Fragments arranged by size: 

                 162     121     115     109      58      39      11       9
            
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     246     624 
   Size:        246     378
 
BsrBI CCG'CTC

Cuts at:      0     300     311     624 
   Size:        300      11     313

  Fragments arranged by size: 

                 313     300      11
 
BsrFI r'CCGG_y

Cuts at:      0     262     440     568     624 
   Size:        262     178     128      56

  Fragments arranged by size: 

                 262     178     128      56
 
BstEII G'GTnAC_C

Cuts at:      0     258     624 
   Size:        258     366
 
BtgI C'CryG_G

Cuts at:      0     281     624 
   Size:        281     343
 
EaeI y'GGCC_r

Cuts at:      0     507     624 
   Size:        507     117
 
EarI CTCTTCn'nnn_

Cuts at:      0     550     624 
   Size:        550      74
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     259     624 
   Size:        259     365
 
EcoICRI GAG'CTC

Cuts at:      0      56     624 
   Size:         56     568
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     324     624 
   Size:        324     300
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0      64     244     624 
   Size:         64     180     380

  Fragments arranged by size: 

                 380     180      64
 
EcoO109I rG'GnC_Cy

Cuts at:      0     276     325     600     624 
   Size:        276      49     275      24

  Fragments arranged by size: 

                 276     275      49      24
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     417     624 
   Size:        417     207
 
HaeII r_GCGC'y

Cuts at:      0     102     465     624 
   Size:        102     363     159

  Fragments arranged by size: 

                 363     159     102
 
MspA1I CmG'CkG

Cuts at:      0     283     624 
   Size:        283     341
 
PasI CC'CwG_GG

Cuts at:      0      60     624 
   Size:         60     564
 
PmlI CAC'GTG

Cuts at:      0     374     624 
   Size:        374     250
 
PpuMI rG'GwC_Cy

Cuts at:      0     276     600     624 
   Size:        276     324      24

  Fragments arranged by size: 

                 324     276      24
 
PspOMI G'GGCC_C

Cuts at:      0     325     624 
   Size:        325     299
 
PstI C_TGCA'G

Cuts at:      0     106     388     624 
   Size:        106     282     236

  Fragments arranged by size: 

                 282     236     106
 
SacI G_AGCT'C

Cuts at:      0      58     624 
   Size:         58     566
 
SacII CC_GC'GG

Cuts at:      0     284     624 
   Size:        284     340
 
SanDI GG'GwC_CC

Cuts at:      0     600     624 
   Size:        600      24
 
SfcI C'TryA_G

Cuts at:      0     102     384     624 
   Size:        102     282     240

  Fragments arranged by size: 

                 282     240     102
 
SgrAI Cr'CCGG_yG

Cuts at:      0     568     624 
   Size:        568      56
 
SmaI CCC'GGG

Cuts at:      0     123     624 
   Size:        123     501
 
SmlI C'TyrA_G

Cuts at:      0      88     624 
   Size:         88     536
 
StyI C'CwwG_G

Cuts at:      0     554     624 
   Size:        554      70
 
TatI w'GTAC_w

Cuts at:      0      35     398     624 
   Size:         35     363     226

  Fragments arranged by size: 

                 363     226      35
 
XmaI C'CCGG_G

Cuts at:      0     121     624 
   Size:        121     503
 
XmnI GAAnn'nnTTC

Cuts at:      0     151     624 
   Size:        151     473
 
ZraI GAC'GTC

Cuts at:      0     419     624 
   Size:        419     205
 

 Enzymes that do cut:

    AatII     AfeI   AflIII     AgeI     ApaI     AvaI     BanI    BanII
     BbsI Bme1580I     BpmI    BpuEI    BsaAI    BsaHI    BsaWI    BseRI
    BseYI  BsiHKAI    BsmBI Bsp1286I    BspMI    BsrBI    BsrFI   BstEII
     BtgI     EaeI     EarI     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I
     FalI    HaeII   MspA1I     PasI     PmlI    PpuMI   PspOMI     PstI
     SacI    SacII    SanDI     SfcI    SgrAI     SmaI     SmlI     StyI
     TatI     XmaI     XmnI     ZraI

 Enzymes that do not cut: 

     AarI     AccI   Acc65I     AclI     AcuI    AflII     AhdI     AleI
     AlfI     AloI    AlwNI    ApaLI     ApoI     AscI     AseI    AsiSI
    AvrII     BaeI    BamHI     BbeI    BbvCI     BcgI    BciVI     BclI
    BfrBI     BglI    BglII     BlpI    BmgBI     BmrI     BmtI     BplI
   Bpu10I     BsaI    BsaBI    BsaXI     BsgI    BsiEI    BsiWI     BsmI
    BspEI    BspHI    BsrDI    BsrGI   BssHII    BssSI   BstAPI    BstBI
    BstXI    BstYI  BstZ17I   Bsu36I    BtgZI     BtsI     ClaI    CspCI
     DraI   DraIII     DrdI     EagI    EcoRI    EcoRV     FseI     FspI
    FspAI    Hin4I   HincII  HindIII     HpaI     KasI     KpnI     MfeI
     MluI     MmeI     MscI     MslI     NaeI     NarI     NcoI     NdeI
   NgoMIV     NheI     NotI     NruI     NsiI     NspI     PacI     PciI
    PflMI     PfoI     PmeI     PpiI    PshAI     PsiI    PspXI     PsrI
     PvuI    PvuII    RsrII     SalI     SapI     SbfI     ScaI    SexAI
     SfiI     SfoI     SgfI    SnaBI     SpeI     SphI     SrfI     SspI
     StuI     SwaI    TaqII     TstI  Tth111I     XbaI     XcmI     XhoI
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