(Circular) (Six-base) MAPSORT of: pF1KB8910.seq Check: 32 from: 1 to: 3737 pF1KB8910 3737 bp With 182 enzymes: SgfI * March 2, 2010 14:29 .. AccI GT'mk_AC Cuts at: 344 775 344 Size: 431 3306 Acc65I G'GTAC_C Cuts at: 753 753 Size: 3737 AclI AA'CG_TT Cuts at: 3577 3577 Size: 3737 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1562 1994 2741 3009 1562 Size: 432 747 268 2290 Fragments arranged by size: 2290 747 432 268 AflIII A'CryG_T Cuts at: 1089 2193 3170 1089 Size: 1104 977 1656 Fragments arranged by size: 1656 1104 977 AgeI A'CCGG_T Cuts at: 2125 2125 Size: 3737 AlwNI CAG_nnn'CTG Cuts at: 608 1042 2609 608 Size: 434 1567 1736 Fragments arranged by size: 1736 1567 434 ApaLI G'TGCA_C Cuts at: 2507 2507 Size: 3737 ApoI r'AATT_y Cuts at: 741 3090 3346 741 Size: 2349 256 1132 Fragments arranged by size: 2349 1132 256 AseI AT'TA_AT Cuts at: 20 20 Size: 3737 AsiSI GCG_AT'CGC Cuts at: 104 104 Size: 3737 AvaI C'yCGr_G Cuts at: 190 277 757 190 Size: 87 480 3170 Fragments arranged by size: 3170 480 87 BamHI G'GATC_C Cuts at: 762 762 Size: 3737 BanI G'GyrC_C Cuts at: 320 753 1167 1415 1450 320 Size: 433 414 248 35 2607 Fragments arranged by size: 2607 433 414 248 35 BanII G_rGCy'C Cuts at: 176 278 293 600 751 1781 176 Size: 102 15 307 151 1030 2132 Fragments arranged by size: 2132 1030 307 151 102 15 BbeI G_GCGC'C Cuts at: 324 1171 1419 324 Size: 847 248 2642 Fragments arranged by size: 2642 847 248 BbsI GAAGACnn'nnnn_ Cuts at: 398 398 Size: 3737 BciVI GTATCCnnnnn_n' Cuts at: 1635 2396 3737 1635 Size: 761 1341 1635 Fragments arranged by size: 1635 1341 761 BclI T'GATC_A Cuts at: 1257 1257 Size: 3737 BglII A'GATC_T Cuts at: 1252 3289 1252 Size: 2037 1700 BlpI GC'TnA_GC Cuts at: 849 849 Size: 3737 Bme1580I G_kGCm'C Cuts at: 284 1362 1455 2511 284 Size: 1078 93 1056 1510 Fragments arranged by size: 1510 1078 1056 93 BmrI ACTGGGnnnn_n' Cuts at: 949 1030 1365 3509 949 Size: 81 335 2144 1177 Fragments arranged by size: 2144 1177 335 81 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2104 2104 Size: 3737 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 235 277 292 649 235 Size: 42 15 357 3323 Fragments arranged by size: 3323 357 42 15 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 160 913 1792 2284 2582 160 Size: 753 879 492 298 1315 Fragments arranged by size: 1315 879 753 492 298 BsaI GGTCTCn'nnnn_ Cuts at: 3441 3441 Size: 3737 BsaAI yAC'GTr Cuts at: 1090 1090 Size: 3737 BsaBI GATnn'nnATC Cuts at: 6 1251 3288 6 Size: 1245 2037 455 Fragments arranged by size: 2037 1245 455 BsaHI Gr'CG_yC Cuts at: 321 1168 1416 2118 3614 321 Size: 847 248 702 1496 444 Fragments arranged by size: 1496 847 702 444 248 BsaWI w'CCGG_w Cuts at: 926 1447 2125 2399 2546 2957 926 Size: 521 678 274 147 411 1706 Fragments arranged by size: 1706 678 521 411 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 229 259 229 Size: 30 3707 BseYI C'CCAG_C Cuts at: 1162 1920 2497 2993 1162 Size: 758 577 496 1906 Fragments arranged by size: 1906 758 577 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 115 244 422 115 Size: 129 178 3430 Fragments arranged by size: 3430 178 129 BsiEI CG_ry'CG Cuts at: 104 371 1279 1325 2533 104 Size: 267 908 46 1208 1308 Fragments arranged by size: 1308 1208 908 267 46 BsiHKAI G_wGCw'C Cuts at: 176 293 751 1529 1719 2511 176 Size: 117 458 778 190 792 1402 Fragments arranged by size: 1402 792 778 458 190 117 Bsp1286I G_dGCh'C Cuts at: 176 278 284 293 600 751 1362 1455 1529 Size: 102 6 9 307 151 611 93 74 Cuts at: 1529 1719 1781 2511 176 Size: 190 62 730 1402 Fragments arranged by size: 1402 730 611 307 190 151 102 93 74 62 9 6 BspEI T'CCGG_A Cuts at: 2957 2957 Size: 3737 BspHI T'CATG_A Cuts at: 2913 2913 Size: 3737 BspMI ACCTGCnnnn'nnnn_ Cuts at: 338 787 1001 1303 1684 338 Size: 449 214 302 381 2391 Fragments arranged by size: 2391 449 381 302 214 BsrBI CCG'CTC Cuts at: 50 230 2029 2083 3567 3732 50 Size: 180 1799 54 1484 165 55 Fragments arranged by size: 1799 1484 180 165 55 54 BsrDI GCAATG_nn' Cuts at: 1649 1649 Size: 3737 BsrFI r'CCGG_y Cuts at: 1735 1916 2125 1735 Size: 181 209 3347 Fragments arranged by size: 3347 209 181 BssSI C'ACGA_G Cuts at: 2008 2366 2008 Size: 358 3379 BstAPI GCAn_nnn'nTGC Cuts at: 1114 1114 Size: 3737 BstBI TT'CG_AA Cuts at: 2098 2098 Size: 3737 BstXI CCAn_nnnn'nTGG Cuts at: 255 255 Size: 3737 BstYI r'GATC_y Cuts at: 1 762 1229 1252 1587 1833 2845 2931 2943 Size: 761 467 23 335 246 1012 86 12 Cuts at: 2943 3289 1 Size: 346 449 Fragments arranged by size: 1012 761 467 449 346 335 246 86 23 12 BtgI C'CryG_G Cuts at: 107 107 Size: 3737 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 1687 1868 2031 1687 Size: 181 163 3393 Fragments arranged by size: 3393 181 163 BtsI GCAGTG_nn' Cuts at: 980 980 Size: 3737 DraI TTT'AAA Cuts at: 736 736 Size: 3737 DraIII CAC_nnn'GTG Cuts at: 138 591 138 Size: 453 3284 DrdI GACnn_nn'nnGTC Cuts at: 1443 2301 1443 Size: 858 2879 EaeI y'GGCC_r Cuts at: 368 1322 1496 1887 1914 3259 368 Size: 954 174 391 27 1345 846 Fragments arranged by size: 1345 954 846 391 174 27 EagI C'GGCC_G Cuts at: 368 1322 368 Size: 954 2783 EarI CTCTTCn'nnn_ Cuts at: 247 1760 1970 247 Size: 1513 210 2014 Fragments arranged by size: 2014 1513 210 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 1945 2255 2401 3547 1945 Size: 310 146 1146 2135 Fragments arranged by size: 2135 1146 310 146 EcoICRI GAG'CTC Cuts at: 174 291 749 174 Size: 117 458 3162 Fragments arranged by size: 3162 458 117 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 235 277 292 649 1562 1994 2741 3009 235 Size: 42 15 357 913 432 747 268 963 Fragments arranged by size: 963 913 747 432 357 268 42 15 EcoO109I rG'GnC_Cy Cuts at: 605 876 605 Size: 271 3466 EcoRI G'AATT_C Cuts at: 741 741 Size: 3737 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 1756 1756 Size: 3737 FspI TGC'GCA Cuts at: 1518 1518 Size: 3737 HaeII r_GCGC'y Cuts at: 324 1171 1419 2441 3420 324 Size: 847 248 1022 979 641 Fragments arranged by size: 1022 979 847 641 248 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 229 261 1579 1609 1611 1641 229 Size: 32 1318 30 2 30 2325 Fragments arranged by size: 2325 1318 32 30 30 2 HincII GTy'rAC Cuts at: 345 776 345 Size: 431 3306 KasI G'GCGC_C Cuts at: 320 1167 1415 320 Size: 847 248 2642 Fragments arranged by size: 2642 847 248 KpnI G_GTAC'C Cuts at: 757 757 Size: 3737 MluI A'CGCG_T Cuts at: 3170 3170 Size: 3737 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 2408 2592 2408 Size: 184 3553 MscI TGG'CCA Cuts at: 1498 1498 Size: 3737 MslI CAynn'nnrTG Cuts at: 1726 1853 1726 Size: 127 3610 MspA1I CmG'CkG Cuts at: 848 1162 1522 2535 2780 848 Size: 314 360 1013 245 1805 Fragments arranged by size: 1805 1013 360 314 245 NaeI GCC'GGC Cuts at: 1918 1918 Size: 3737 NarI GG'CG_CC Cuts at: 321 1168 1416 321 Size: 847 248 2642 Fragments arranged by size: 2642 847 248 NcoI C'CATG_G Cuts at: 107 107 Size: 3737 NdeI CA'TA_TG Cuts at: 418 418 Size: 3737 NgoMIV G'CCGG_C Cuts at: 1916 1916 Size: 3737 NspI r_CATG'y Cuts at: 790 1290 2197 790 Size: 500 907 2330 Fragments arranged by size: 2330 907 500 PciI A'CATG_T Cuts at: 2193 2193 Size: 3737 PflMI CCAn_nnn'nTGG Cuts at: 591 591 Size: 3737 PmeI GTTT'AAAC Cuts at: 736 736 Size: 3737 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 163 195 2876 2908 163 Size: 32 2681 32 992 Fragments arranged by size: 2681 992 32 32 PpuMI rG'GwC_Cy Cuts at: 605 605 Size: 3737 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 629 661 629 Size: 32 3705 PstI C_TGCA'G Cuts at: 784 1469 784 Size: 685 3052 PvuI CG_AT'CG Cuts at: 104 104 Size: 3737 PvuII CAG'CTG Cuts at: 1162 1522 1162 Size: 360 3377 RsrII CG'GwC_CG Cuts at: 1932 1932 Size: 3737 SacI G_AGCT'C Cuts at: 176 293 751 176 Size: 117 458 3162 Fragments arranged by size: 3162 458 117 SalI G'TCGA_C Cuts at: 343 774 343 Size: 431 3306 SapI GCTCTTCn'nnn_ Cuts at: 1760 1970 1760 Size: 210 3527 SbfI CC_TGCA'GG Cuts at: 784 784 Size: 3737 ScaI AGT'ACT Cuts at: 3254 3254 Size: 3737 SexAI A'CCwGG_T Cuts at: 587 620 632 587 Size: 33 12 3692 Fragments arranged by size: 3692 33 12 SfcI C'TryA_G Cuts at: 33 780 1465 2458 2649 33 Size: 747 685 993 191 1121 Fragments arranged by size: 1121 993 747 685 191 SfoI GGC'GCC Cuts at: 322 1169 1417 322 Size: 847 248 2642 Fragments arranged by size: 2642 847 248 SgfI GCG_AT'CGC Cuts at: 104 104 Size: 3737 SmaI CCC'GGG Cuts at: 279 759 279 Size: 480 3257 SmlI C'TyrA_G Cuts at: 175 892 1807 2299 2561 175 Size: 717 915 492 262 1351 Fragments arranged by size: 1351 915 717 492 262 SpeI A'CTAG_T Cuts at: 67 67 Size: 3737 SphI G_CATG'C Cuts at: 790 1290 790 Size: 500 3237 SrfI GCCC'GGGC Cuts at: 279 279 Size: 3737 StyI C'CwwG_G Cuts at: 107 871 1225 107 Size: 764 354 2619 Fragments arranged by size: 2619 764 354 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 184 1320 1453 1912 2121 184 Size: 1136 133 459 209 1800 Fragments arranged by size: 1800 1136 459 209 133 TatI w'GTAC_w Cuts at: 3252 3252 Size: 3737 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3254 3286 3254 Size: 32 3705 Tth111I GACn'n_nGTC Cuts at: 1534 1534 Size: 3737 XbaI T'CTAG_A Cuts at: 768 768 Size: 3737 XcmI CCAnnnn_n'nnnnTGG Cuts at: 673 1232 673 Size: 559 3178 XmaI C'CCGG_G Cuts at: 277 757 277 Size: 480 3257 Enzymes that do cut: AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BciVI BclI BglII BlpI Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI BtgI BtgZI BtsI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NspI PciI PflMI PmeI PpiI PpuMI PsrI PstI PvuI PvuII RsrII SacI SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI SmaI SmlI SpeI SphI SrfI StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI Enzymes that do not cut: AarI AatII AfeI AflII AhdI AleI AlfI AloI ApaI AscI AvrII BaeI BbvCI BcgI BfrBI BglI BmgBI BmtI Bpu10I BseRI BsiWI BsmI BsmBI BsrGI BssHII BstEII BstZ17I Bsu36I ClaI CspCI EcoNI EcoRV FseI FspAI HindIII HpaI MfeI NheI NotI NruI NsiI PacI PasI PfoI PmlI PshAI PsiI PspOMI PspXI SacII SanDI SfiI SgrAI SnaBI SspI StuI SwaI XhoI XmnI ZraI