Restriction map for pF1KB8910
 (Linear) (Six-base) MAPSORT of: KIBB8910.seq  Check: 9010  from: 1  to: 624



KIBB8910.orf

 With 182 enzymes: SgfI * 

                        March 2, 2010 14:29  ..

AccI GT'mk_AC

Cuts at:      0     236     624 
   Size:        236     388
 
AlwNI CAG_nnn'CTG

Cuts at:      0     500     624 
   Size:        500     124
 
AvaI C'yCGr_G

Cuts at:      0      82     169     624 
   Size:         82      87     455

  Fragments arranged by size: 

                 455      87      82
 
BanI G'GyrC_C

Cuts at:      0     212     624 
   Size:        212     412
 
BanII G_rGCy'C

Cuts at:      0      68     170     185     492     624 
   Size:         68     102      15     307     132

  Fragments arranged by size: 

                 307     132     102      68      15
 
BbeI G_GCGC'C

Cuts at:      0     216     624 
   Size:        216     408
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     290     624 
   Size:        290     334
 
Bme1580I G_kGCm'C

Cuts at:      0     176     624 
   Size:        176     448
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     127     169     184     541     624 
   Size:        127      42      15     357      83

  Fragments arranged by size: 

                 357     127      83      42      15
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      52     624 
   Size:         52     572
 
BsaHI Gr'CG_yC

Cuts at:      0     213     624 
   Size:        213     411
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     121     151     624 
   Size:        121      30     473

  Fragments arranged by size: 

                 473     121      30
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0       7     136     314     624 
   Size:          7     129     178     310

  Fragments arranged by size: 

                 310     178     129       7
 
BsiEI CG_ry'CG

Cuts at:      0     263     624 
   Size:        263     361
 
BsiHKAI G_wGCw'C

Cuts at:      0      68     185     624 
   Size:         68     117     439

  Fragments arranged by size: 

                 439     117      68
 
Bsp1286I G_dGCh'C

Cuts at:      0      68     170     176     185     492     624 
   Size:         68     102       6       9     307     132

  Fragments arranged by size: 

                 307     132     102      68       9       6
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     230     624 
   Size:        230     394
 
BsrBI CCG'CTC

Cuts at:      0     122     624 
   Size:        122     502
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     147     624 
   Size:        147     477
 
DraIII CAC_nnn'GTG

Cuts at:      0      30     483     624 
   Size:         30     453     141

  Fragments arranged by size: 

                 453     141      30
 
EaeI y'GGCC_r

Cuts at:      0     260     624 
   Size:        260     364
 
EagI C'GGCC_G

Cuts at:      0     260     624 
   Size:        260     364
 
EarI CTCTTCn'nnn_

Cuts at:      0     139     624 
   Size:        139     485
 
EcoICRI GAG'CTC

Cuts at:      0      66     183     624 
   Size:         66     117     441

  Fragments arranged by size: 

                 441     117      66
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     127     169     184     541     624 
   Size:        127      42      15     357      83

  Fragments arranged by size: 

                 357     127      83      42      15
 
EcoO109I rG'GnC_Cy

Cuts at:      0     497     624 
   Size:        497     127
 
HaeII r_GCGC'y

Cuts at:      0     216     624 
   Size:        216     408
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     121     153     624 
   Size:        121      32     471

  Fragments arranged by size: 

                 471     121      32
 
HincII GTy'rAC

Cuts at:      0     237     624 
   Size:        237     387
 
KasI G'GCGC_C

Cuts at:      0     212     624 
   Size:        212     412
 
NarI GG'CG_CC

Cuts at:      0     213     624 
   Size:        213     411
 
NdeI CA'TA_TG

Cuts at:      0     310     624 
   Size:        310     314
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     483     624 
   Size:        483     141
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0      55      87     624 
   Size:         55      32     537

  Fragments arranged by size: 

                 537      55      32
 
PpuMI rG'GwC_Cy

Cuts at:      0     497     624 
   Size:        497     127
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0     521     553     624 
   Size:        521      32      71

  Fragments arranged by size: 

                 521      71      32
 
SacI G_AGCT'C

Cuts at:      0      68     185     624 
   Size:         68     117     439

  Fragments arranged by size: 

                 439     117      68
 
SalI G'TCGA_C

Cuts at:      0     235     624 
   Size:        235     389
 
SexAI A'CCwGG_T

Cuts at:      0     479     512     524     624 
   Size:        479      33      12     100

  Fragments arranged by size: 

                 479     100      33      12
 
SfoI GGC'GCC

Cuts at:      0     214     624 
   Size:        214     410
 
SmaI CCC'GGG

Cuts at:      0     171     624 
   Size:        171     453
 
SmlI C'TyrA_G

Cuts at:      0      67     624 
   Size:         67     557
 
SrfI GCCC'GGGC

Cuts at:      0     171     624 
   Size:        171     453
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0      76     624 
   Size:         76     548
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     565     624 
   Size:        565      59
 
XmaI C'CCGG_G

Cuts at:      0     169     624 
   Size:        169     455
 

 Enzymes that do cut:

     AccI    AlwNI     AvaI     BanI    BanII     BbeI     BbsI Bme1580I
     BpmI    BpuEI    BsaHI    BsaXI     BsgI    BsiEI  BsiHKAI Bsp1286I
    BspMI    BsrBI    BstXI   DraIII     EaeI     EagI     EarI  EcoICRI
  Eco57MI EcoO109I    HaeII    Hin4I   HincII     KasI     NarI     NdeI
    PflMI     PpiI    PpuMI     PsrI     SacI     SalI    SexAI     SfoI
     SmaI     SmlI     SrfI    TaqII     XcmI     XmaI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AcuI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI     ApaI    ApaLI     ApoI
     AscI     AseI    AsiSI    AvrII     BaeI    BamHI    BbvCI     BcgI
    BciVI     BclI    BfrBI     BglI    BglII     BlpI    BmgBI     BmrI
     BmtI     BplI   Bpu10I     BsaI    BsaAI    BsaBI    BsaWI    BseRI
    BseYI    BsiWI     BsmI    BsmBI    BspEI    BspHI    BsrDI    BsrFI
    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII    BstYI  BstZ17I
   Bsu36I     BtgI    BtgZI     BtsI     ClaI    CspCI     DraI     DrdI
     EciI    EcoNI    EcoRI    EcoRV     FalI     FseI     FspI    FspAI
  HindIII     HpaI     KpnI     MfeI     MluI     MmeI     MscI     MslI
   MspA1I     NaeI     NcoI   NgoMIV     NheI     NotI     NruI     NsiI
     NspI     PacI     PasI     PciI     PfoI     PmeI     PmlI    PshAI
     PsiI   PspOMI    PspXI     PstI     PvuI    PvuII    RsrII    SacII
    SanDI     SapI     SbfI     ScaI     SfcI     SfiI     SgfI    SgrAI
    SnaBI     SpeI     SphI     SspI     StuI     StyI     SwaI     TatI
     TstI  Tth111I     XbaI     XhoI     XmnI     ZraI
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