(Circular) (Six-base) MAPSORT of: pF1KE1345.seq Check: 7016 from: 1 to: 3905 pF1KE1345 3905 bp With 182 enzymes: SgfI * February 23, 2015 11:06 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 507 507 Size: 3905 AccI GT'mk_AC Cuts at: 943 943 Size: 3905 Acc65I G'GTAC_C Cuts at: 921 921 Size: 3905 AclI AA'CG_TT Cuts at: 3745 3745 Size: 3905 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 421 544 688 1730 2162 2909 3177 421 Size: 123 144 1042 432 747 268 1149 Fragments arranged by size: 1149 1042 747 432 268 144 123 AflII C'TTAA_G Cuts at: 220 220 Size: 3905 AflIII A'CryG_T Cuts at: 1257 2361 3338 1257 Size: 1104 977 1824 Fragments arranged by size: 1824 1104 977 AgeI A'CCGG_T Cuts at: 2293 2293 Size: 3905 AhdI GACnn_n'nnGTC Cuts at: 508 508 Size: 3905 AlwNI CAG_nnn'CTG Cuts at: 378 387 522 552 1210 2777 378 Size: 9 135 30 658 1567 1506 Fragments arranged by size: 1567 1506 658 135 30 9 ApaLI G'TGCA_C Cuts at: 2675 2675 Size: 3905 ApoI r'AATT_y Cuts at: 909 3258 3514 909 Size: 2349 256 1300 Fragments arranged by size: 2349 1300 256 AseI AT'TA_AT Cuts at: 20 20 Size: 3905 AsiSI GCG_AT'CGC Cuts at: 104 104 Size: 3905 AvaI C'yCGr_G Cuts at: 158 925 158 Size: 767 3138 BamHI G'GATC_C Cuts at: 930 930 Size: 3905 BanI G'GyrC_C Cuts at: 921 1335 1583 1618 921 Size: 414 248 35 3208 Fragments arranged by size: 3208 414 248 35 BanII G_rGCy'C Cuts at: 919 1949 919 Size: 1030 2875 BbeI G_GCGC'C Cuts at: 1339 1587 1339 Size: 248 3657 BciVI GTATCCnnnnn_n' Cuts at: 1803 2564 3905 1803 Size: 761 1341 1803 Fragments arranged by size: 1803 1341 761 BclI T'GATC_A Cuts at: 689 1425 689 Size: 736 3169 BglII A'GATC_T Cuts at: 1420 3457 1420 Size: 2037 1868 BlpI GC'TnA_GC Cuts at: 589 886 1017 589 Size: 297 131 3477 Fragments arranged by size: 3477 297 131 Bme1580I G_kGCm'C Cuts at: 1530 1623 2679 1530 Size: 93 1056 2756 Fragments arranged by size: 2756 1056 93 BmrI ACTGGGnnnn_n' Cuts at: 427 562 1117 1198 1533 3677 427 Size: 135 555 81 335 2144 655 Fragments arranged by size: 2144 655 555 335 135 81 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 837 2272 837 Size: 1435 2470 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 409 726 409 Size: 317 3588 Bpu10I CC'TnA_GC Cuts at: 646 646 Size: 3905 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 688 1081 1960 2452 2750 688 Size: 393 879 492 298 1843 Fragments arranged by size: 1843 879 492 393 298 BsaI GGTCTCn'nnnn_ Cuts at: 269 3609 269 Size: 3340 565 BsaAI yAC'GTr Cuts at: 1258 1258 Size: 3905 BsaBI GATnn'nnATC Cuts at: 6 289 1419 3456 6 Size: 283 1130 2037 455 Fragments arranged by size: 2037 1130 455 283 BsaHI Gr'CG_yC Cuts at: 1336 1584 2286 3782 1336 Size: 248 702 1496 1459 Fragments arranged by size: 1496 1459 702 248 BsaWI w'CCGG_w Cuts at: 1094 1615 2293 2567 2714 3125 1094 Size: 521 678 274 147 411 1874 Fragments arranged by size: 1874 678 521 411 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 481 511 481 Size: 30 3875 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 310 505 310 Size: 195 3710 BseYI C'CCAG_C Cuts at: 1330 2088 2665 3161 1330 Size: 758 577 496 2074 Fragments arranged by size: 2074 758 577 496 BsiEI CG_ry'CG Cuts at: 104 1447 1493 2701 104 Size: 1343 46 1208 1308 Fragments arranged by size: 1343 1308 1208 46 BsiHKAI G_wGCw'C Cuts at: 590 919 1697 1887 2679 590 Size: 329 778 190 792 1816 Fragments arranged by size: 1816 792 778 329 190 Bsp1286I G_dGCh'C Cuts at: 590 919 1530 1623 1697 1887 1949 2679 590 Size: 329 611 93 74 190 62 730 1816 Fragments arranged by size: 1816 730 611 329 190 93 74 62 BspEI T'CCGG_A Cuts at: 3125 3125 Size: 3905 BspHI T'CATG_A Cuts at: 3081 3081 Size: 3905 BspMI ACCTGCnnnn'nnnn_ Cuts at: 287 386 507 955 1169 1471 1852 287 Size: 99 121 448 214 302 381 2340 Fragments arranged by size: 2340 448 381 302 214 121 99 BsrBI CCG'CTC Cuts at: 50 2197 2251 3735 3900 50 Size: 2147 54 1484 165 55 Fragments arranged by size: 2147 1484 165 55 54 BsrDI GCAATG_nn' Cuts at: 1817 1817 Size: 3905 BsrFI r'CCGG_y Cuts at: 1903 2084 2293 1903 Size: 181 209 3515 Fragments arranged by size: 3515 209 181 BssSI C'ACGA_G Cuts at: 2176 2534 2176 Size: 358 3547 BstAPI GCAn_nnn'nTGC Cuts at: 1282 1282 Size: 3905 BstBI TT'CG_AA Cuts at: 2266 2266 Size: 3905 BstEII G'GTnAC_C Cuts at: 438 438 Size: 3905 BstYI r'GATC_y Cuts at: 1 930 1397 1420 1755 2001 3013 3099 3111 Size: 929 467 23 335 246 1012 86 12 Cuts at: 3111 3457 1 Size: 346 449 Fragments arranged by size: 1012 929 467 449 346 335 246 86 23 12 BtgI C'CryG_G Cuts at: 107 842 107 Size: 735 3170 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 1855 2036 2199 1855 Size: 181 163 3561 Fragments arranged by size: 3561 181 163 BtsI GCAGTG_nn' Cuts at: 1148 1148 Size: 3905 DraI TTT'AAA Cuts at: 904 904 Size: 3905 DraIII CAC_nnn'GTG Cuts at: 599 599 Size: 3905 DrdI GACnn_nn'nnGTC Cuts at: 1611 2469 1611 Size: 858 3047 EaeI y'GGCC_r Cuts at: 845 1490 1664 2055 2082 3427 845 Size: 645 174 391 27 1345 1323 Fragments arranged by size: 1345 1323 645 391 174 27 EagI C'GGCC_G Cuts at: 1490 1490 Size: 3905 EarI CTCTTCn'nnn_ Cuts at: 1928 2138 1928 Size: 210 3695 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 2113 2423 2569 3715 2113 Size: 310 146 1146 2303 Fragments arranged by size: 2303 1146 310 146 EcoICRI GAG'CTC Cuts at: 917 917 Size: 3905 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 409 421 544 688 726 1730 2162 2909 3177 Size: 12 123 144 38 1004 432 747 268 Cuts at: 3177 409 Size: 1137 Fragments arranged by size: 1137 1004 747 432 268 144 123 38 12 EcoNI CCTnn'n_nnAGG Cuts at: 636 636 Size: 3905 EcoO109I rG'GnC_Cy Cuts at: 1044 1044 Size: 3905 EcoRI G'AATT_C Cuts at: 909 909 Size: 3905 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 851 1924 851 Size: 1073 2832 FspI TGC'GCA Cuts at: 1686 1686 Size: 3905 HaeII r_GCGC'y Cuts at: 646 1339 1587 2609 3588 646 Size: 693 248 1022 979 963 Fragments arranged by size: 1022 979 963 693 248 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 280 312 494 526 1747 1777 1779 1809 280 Size: 32 182 32 1221 30 2 30 2376 Fragments arranged by size: 2376 1221 182 32 32 30 30 2 HincII GTy'rAC Cuts at: 944 944 Size: 3905 KasI G'GCGC_C Cuts at: 1335 1583 1335 Size: 248 3657 KpnI G_GTAC'C Cuts at: 925 925 Size: 3905 MluI A'CGCG_T Cuts at: 3338 3338 Size: 3905 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 2576 2760 2576 Size: 184 3721 MscI TGG'CCA Cuts at: 847 1666 847 Size: 819 3086 MslI CAynn'nnrTG Cuts at: 1894 2021 1894 Size: 127 3778 MspA1I CmG'CkG Cuts at: 1016 1330 1690 2703 2948 1016 Size: 314 360 1013 245 1973 Fragments arranged by size: 1973 1013 360 314 245 NaeI GCC'GGC Cuts at: 2086 2086 Size: 3905 NarI GG'CG_CC Cuts at: 1336 1584 1336 Size: 248 3657 NcoI C'CATG_G Cuts at: 107 842 107 Size: 735 3170 NgoMIV G'CCGG_C Cuts at: 2084 2084 Size: 3905 NspI r_CATG'y Cuts at: 958 1458 2365 958 Size: 500 907 2498 Fragments arranged by size: 2498 907 500 PasI CC'CwG_GG Cuts at: 753 753 Size: 3905 PciI A'CATG_T Cuts at: 2361 2361 Size: 3905 PflMI CCAn_nnn'nTGG Cuts at: 552 552 Size: 3905 PmeI GTTT'AAAC Cuts at: 904 904 Size: 3905 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 721 753 3044 3076 721 Size: 32 2291 32 1550 Fragments arranged by size: 2291 1550 32 32 PstI C_TGCA'G Cuts at: 207 383 640 952 1637 207 Size: 176 257 312 685 2475 Fragments arranged by size: 2475 685 312 257 176 PvuI CG_AT'CG Cuts at: 104 104 Size: 3905 PvuII CAG'CTG Cuts at: 1330 1690 1330 Size: 360 3545 RsrII CG'GwC_CG Cuts at: 2100 2100 Size: 3905 SacI G_AGCT'C Cuts at: 919 919 Size: 3905 SalI G'TCGA_C Cuts at: 942 942 Size: 3905 SapI GCTCTTCn'nnn_ Cuts at: 1928 2138 1928 Size: 210 3695 SbfI CC_TGCA'GG Cuts at: 383 952 383 Size: 569 3336 ScaI AGT'ACT Cuts at: 3422 3422 Size: 3905 SfcI C'TryA_G Cuts at: 33 203 379 636 948 1633 2626 2817 33 Size: 170 176 257 312 685 993 191 1121 Fragments arranged by size: 1121 993 685 312 257 191 176 170 SfoI GGC'GCC Cuts at: 1337 1585 1337 Size: 248 3657 SgfI GCG_AT'CGC Cuts at: 104 104 Size: 3905 SmaI CCC'GGG Cuts at: 927 927 Size: 3905 SmlI C'TyrA_G Cuts at: 220 703 1060 1975 2467 2729 220 Size: 483 357 915 492 262 1396 Fragments arranged by size: 1396 915 492 483 357 262 SpeI A'CTAG_T Cuts at: 67 67 Size: 3905 SphI G_CATG'C Cuts at: 958 1458 958 Size: 500 3405 SspI AAT'ATT Cuts at: 178 178 Size: 3905 StuI AGG'CCT Cuts at: 418 418 Size: 3905 StyI C'CwwG_G Cuts at: 107 128 842 1039 1393 107 Size: 21 714 197 354 2619 Fragments arranged by size: 2619 714 354 197 21 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 1488 1621 2080 2289 1488 Size: 133 459 209 3104 Fragments arranged by size: 3104 459 209 133 TatI w'GTAC_w Cuts at: 3420 3420 Size: 3905 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3422 3454 3422 Size: 32 3873 Tth111I GACn'n_nGTC Cuts at: 1702 1702 Size: 3905 XbaI T'CTAG_A Cuts at: 936 936 Size: 3905 XcmI CCAnnnn_n'nnnnTGG Cuts at: 750 1400 750 Size: 650 3255 XmaI C'CCGG_G Cuts at: 925 925 Size: 3905 Enzymes that do cut: AarI AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BciVI BclI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI PasI PciI PflMI PmeI PpiI PstI PvuI PvuII RsrII SacI SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI Enzymes that do not cut: AatII AfeI AleI AlfI AloI ApaI AscI AvrII BaeI BbsI BbvCI BcgI BfrBI BglI BmgBI BmtI BsgI BsiWI BsmI BsmBI BsrGI BssHII BstXI BstZ17I Bsu36I ClaI CspCI EcoRV FseI FspAI HindIII HpaI MfeI NdeI NheI NotI NruI NsiI PacI PfoI PmlI PpuMI PshAI PsiI PspOMI PspXI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI SwaI XhoI XmnI ZraI