Restriction map for pF1KB6602
##################################
# NO CUT ENZYMES (  112 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcsI        AflII       AflIII      Alw44I      Aor51HI     
ApaLI       ApoI        AscI        AseI        AsnI        
Asp700I     Asp718I     AspI        AviII       AvrII       
BbrPI       BbsI        BcgI        BciVI       BclI        
BfrI        BglI        BlnI        BlpI        Bpu1102I    
BpuAI       BsaAI       BsaBI       BseAI       BsiWI       
BsmI        Bsp1407I    BspDI       BspEI       BspLU11I    
BspMI       BsrDI       BsrGI       BssHII      BssSI       
Bst1107I    BstAPI      BstBI       BstZ17I     CelII       
ClaI        CpoI        DraI        DraIII      Eco47III    
EcoRI       EcoRV       EcoT22I     FbaI        FseI        
FspI        HindIII     HpaI        KpnI        KspI        
MamI        MfeI        MluI        MroI        MunI        
NaeI        NdeI        NgoMIV      NheI        NotI        
NruI        NsiI        NspI        NspV        PacI        
PaeR7I      PflFI       PmaCI       PmeI        PmlI        
PshAI       PshBI       Psp1406I    PvuI        RsrII       
SacI        SacII       SalI        SbfI        SexAI       
SfiI        SfuI        SgrAI       SnaBI       SpeI        
SphI        Sse8387I    SspBI       SspI        SwaI        
TfiI        Tsp509I     Tth111I     XbaI        XcmI        
XhoI        XmnI        


###################################
# ONE CUT ENZYMES (   44 enzymes) #
###################################

AccII          93  CGCG                 CG^CG
AgeI           38  ACCGGT               A^CCGGT
ApaI          758  GGGCCC               GGGCC^C
AvaI         1055  CCCGGG               C^[CT]CG[GA]G
BamHI        1019  GGATCC               G^GATCC
BbvCI         859  GCTGAGG              CCTCAGC(-5/-2)
BfaI          799  CTAG                 C^TAG
BglII         416  AGATCT               A^GATCT
BsgI         1027  CTGCAC               GTGCAG(16/14)
BsoBI        1055  CCCGGG               C^[CT]CG[GA]G
BspHI         635  TCATGA               T^CATGA
BsrBI         147  CCGCTC               CCGCTC(-3/-3)
BstEII        843  GGTCACC              G^GT[ACGT]ACC
BstPI         843  GGTCACC              G^GT[ACGT]ACC
BstUI          93  CGCG                 CG^CG
BstXI         737  CCAAGATCATGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DrdI          487  GACTTTAGAGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI           79  CGGCCG               C^GGCCG
EclXI          79  CGGCCG               C^GGCCG
Eco52I         79  CGGCCG               C^GGCCG
EcoNI         969  CCTGAGGGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I       843  GGTCACC              G^GT[ACGT]ACC
HincII        696  GTTGAC               GT[CT]^[GA]AC
HindII        696  GTTGAC               GT[CT]^[GA]AC
MaeI          799  CTAG                 C^TAG
MaeII         900  ACGT                 A^CGT
MaeIII        844  GTCAC                ^GT[ACGT]AC
MseI         1169  TTAA                 T^TAA
MslI          834  CACCACAGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I        592  CAGCTG               C[AC]G^C[GT]G
MvnI           93  CGCG                 CG^CG
NcoI          546  CCATGG               C^CATGG
PinAI          38  ACCGGT               A^CCGGT
PvuII         592  CAGCTG               CAG^CTG
RcaI          635  TCATGA               T^CATGA
SapI          630  GCTCTTC              GCTCTTC(1/4)
ScaI          806  AGTACT               AGT^ACT
SmaI         1055  CCCGGG               CCC^GGG
StuI          666  AGGCCT               AGG^CCT
Tru9I        1169  TTAA                 T^TAA
Tsp45I        844  GTCAC                ^GT[GC]AC
TspRI         839  CAGTG                CA[GC]TG[ACGT][ACGT]^
XmaCI        1055  CCCGGG               C^CCGGG
XmaI         1055  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   35 enzymes) #
###################################

AcyI           60  GGCGCC               G[GA]^CG[CT]C
               73  GGCGCC              
AfaI          807  GTAC                 GT^AC
              902  GTAC                
AhdI          359  GACCCCAGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
              899  GACGTACAGTC         
AlwNI         110  CAGGCGCTG            CAG[ACGT][ACGT][ACGT]^CTG
              369  CAGAGACTG           
AocI          405  CCTGAGG              CC^T[ACGT]AGG
              969  CCTGAGG             
AspEI         359  GACCCCAGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
              899  GACGTACAGTC         
AspHI         340  GAGCAC               G[AT]GC[AT]^C
              831  GAGCAC              
BalI          188  TGGCCA               TGG^CCA
              515  TGGCCA              
BanI           60  GGCGCC               G^G[CT][GA]CC
               73  GGCGCC              
BbeI           60  GGCGCC               GGCGC^C
               73  GGCGCC              
BsaHI          60  GGCGCC               G[GA]^CG[CT]C
               73  GGCGCC              
BsaWI          38  ACCGGT               [AT]^CCGG[AT]
              314  ACCGGA              
BsiEI          79  CGGCCG               CG[GA][CT]^CG
              264  CGACCG              
BsiHKAI       340  GAGCAC               G[AT]GC[AT]^C
              831  GAGCAC              
BsmBI         877  GAGACG               CGTCTC(1/5)
              897  GAGACG              
BsrFI          38  ACCGGT               [GA]^CCGG[CT]
               81  GCCGGT              
Bsu36I        405  CCTGAGG              CC^T[ACGT]AGG
              969  CCTGAGG             
Cfr10I         38  ACCGGT               [GA]^CCGG[CT]
               81  GCCGGT              
DsaI          546  CCATGG               C^C[GA][CT]GG
              701  CCGTGG              
Eam1105I      359  GACCCCAGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
              899  GACGTACAGTC         
EarI          631  CTCTTC               CTCTTC(1/4)
              652  GAAGAG              
Eco81I        405  CCTGAGG              CC^T[ACGT]AGG
              969  CCTGAGG             
HgaI          882  GACGC                GACGC(5/10)
              101  GCGTC               
Hin1I          60  GGCGCC               G[GA]^CG[CT]C
               73  GGCGCC              
KasI           60  GGCGCC               G^GCGCC
               73  GGCGCC              
Ksp632I       631  CTCTTC               CTCTTC(1/4)
              652  GAAGAG              
MluNI         188  TGGCCA               TGG^CCA
              515  TGGCCA              
MscI          188  TGGCCA               TGG^CCA
              515  TGGCCA              
NarI           60  GGCGCC               GG^CGCC
               73  GGCGCC              
PflMI         525  CCAGTCTGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              909  CCAGTCCTTGG         
PstI          454  CTGCAG               CTGCA^G
              589  CTGCAG              
RsaI          807  GTAC                 GT^AC
              902  GTAC                
SfoI           60  GGCGCC               GGC^GCC
               73  GGCGCC              
SmlI          616  CTCAAG               C^T[CT][GA]AG
             1033  CTTGAG              
Van91I        525  CCAGTCTGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              909  CCAGTCCTTGG         


#########################################
# 3 OR MORE CUT ENZYMES (   67 enzymes) #
#########################################

AciI        AluI        AlwI        AvaII       BanII       
BbvI        BcnI        BmyI        BpmI        BsaI        
BsaJI       BseRI       BsiYI       BslI        BsmAI       
BsmFI       Bsp1286I    BsrI        BssKI       BstF5I      
BstNI       BstYI       Cac8I       CfoI        Cfr13I      
DdeI        DpnII       DraII       EaeI        EcoO109I    
EcoRII      EcoT14I     FauI        Fnu4HI      FokI        
HaeII       HaeIII      HapII       HhaI        HinP1I      
HinfI       HpaII       HphI        ItaI        MboI        
MboII       MflI        MnlI        MspI        MvaI        
MwoI        NciI        NdeII       NlaIII      NlaIV       
PleI        PpuMI       Sau3AI      Sau96I      ScrFI       
SfaNI       SfcI        StyI        TaqI        TseI        
TthHB8I     XhoII       
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