Restriction map for pF1KB6602
 (Linear) (Six-base) MAPSORT of: KIBB6602.seq  Check: 9068  from: 1  to: 1290



KIBB6602.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:21  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     144     967    1290 
   Size:        144     823     323

  Fragments arranged by size: 

                 823     323     144
 
AgeI A'CCGG_T

Cuts at:      0      38    1290 
   Size:         38    1252
 
AhdI GACnn_n'nnGTC

Cuts at:      0     364     904    1290 
   Size:        364     540     386

  Fragments arranged by size: 

                 540     386     364
 
AleI CACnn'nnGTG

Cuts at:      0     838    1290 
   Size:        838     452
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     469    1219    1290 
   Size:        469     750      71

  Fragments arranged by size: 

                 750     469      71
 
AlwNI CAG_nnn'CTG

Cuts at:      0     115     374    1290 
   Size:        115     259     916

  Fragments arranged by size: 

                 916     259     115
 
ApaI G_GGCC'C

Cuts at:      0     762    1290 
   Size:        762     528
 
AvaI C'yCGr_G

Cuts at:      0    1055    1290 
   Size:       1055     235
 
BamHI G'GATC_C

Cuts at:      0    1019    1290 
   Size:       1019     271
 
BanI G'GyrC_C

Cuts at:      0      60      73    1290 
   Size:         60      13    1217

  Fragments arranged by size: 

                1217      60      13
 
BanII G_rGCy'C

Cuts at:      0      52     124     414     554     762    1290 
   Size:         52      72     290     140     208     528

  Fragments arranged by size: 

                 528     290     208     140      72      52
 
BbeI G_GCGC'C

Cuts at:      0      64      77    1290 
   Size:         64      13    1213

  Fragments arranged by size: 

                1213      64      13
 
BbvCI CC'TCA_GC

Cuts at:      0     860    1290 
   Size:        860     430
 
BglII A'GATC_T

Cuts at:      0     416    1290 
   Size:        416     874
 
Bme1580I G_kGCm'C

Cuts at:      0     762    1290 
   Size:        762     528
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     322     343     396     616    1138    1290 
   Size:        322      21      53     220     522     152

  Fragments arranged by size: 

                 522     322     220     152      53      21
 
Bpu10I CC'TnA_GC

Cuts at:      0     860    1290 
   Size:        860     430
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     601    1054    1290 
   Size:        601     453     236

  Fragments arranged by size: 

                 601     453     236
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     238     298     818    1255    1290 
   Size:        238      60     520     437      35

  Fragments arranged by size: 

                 520     437     238      60      35
 
BsaHI Gr'CG_yC

Cuts at:      0      61      74    1290 
   Size:         61      13    1216

  Fragments arranged by size: 

                1216      61      13
 
BsaWI w'CCGG_w

Cuts at:      0      38     314    1290 
   Size:         38     276     976

  Fragments arranged by size: 

                 976     276      38
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     916     946    1290 
   Size:        916      30     344

  Fragments arranged by size: 

                 916     344      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     309     637     808     841    1290 
   Size:        309     328     171      33     449

  Fragments arranged by size: 

                 449     328     309     171      33
 
BseYI C'CCAG_C

Cuts at:      0      64     115    1290 
   Size:         64      51    1175

  Fragments arranged by size: 

                1175      64      51
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1012    1290 
   Size:       1012     278
 
BsiEI CG_ry'CG

Cuts at:      0      82     267    1290 
   Size:         82     185    1023

  Fragments arranged by size: 

                1023     185      82
 
BsiHKAI G_wGCw'C

Cuts at:      0     344     835    1290 
   Size:        344     491     455

  Fragments arranged by size: 

                 491     455     344
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     871     891    1290 
   Size:        871      20     399

  Fragments arranged by size: 

                 871     399      20
 
Bsp1286I G_dGCh'C

Cuts at:      0      52     124     344     414     554     762     835    1290 
   Size:         52      72     220      70     140     208      73     455

  Fragments arranged by size: 

                 455     220     208     140      73      72      70      52
            
 
BspHI T'CATG_A

Cuts at:      0     635    1290 
   Size:        635     655
 
BsrBI CCG'CTC

Cuts at:      0     149    1290 
   Size:        149    1141
 
BsrFI r'CCGG_y

Cuts at:      0      38      81    1290 
   Size:         38      43    1209

  Fragments arranged by size: 

                1209      43      38
 
BstEII G'GTnAC_C

Cuts at:      0     843    1290 
   Size:        843     447
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     744    1290 
   Size:        744     546
 
BstYI r'GATC_y

Cuts at:      0     134     416    1019    1290 
   Size:        134     282     603     271

  Fragments arranged by size: 

                 603     282     271     134
 
Bsu36I CC'TnA_GG

Cuts at:      0     406     970    1290 
   Size:        406     564     320

  Fragments arranged by size: 

                 564     406     320
 
BtgI C'CryG_G

Cuts at:      0     546     701    1290 
   Size:        546     155     589

  Fragments arranged by size: 

                 589     546     155
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     413     448    1290 
   Size:        413      35     842

  Fragments arranged by size: 

                 842     413      35
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     493    1290 
   Size:        493     797
 
EaeI y'GGCC_r

Cuts at:      0      79      95     188     515    1290 
   Size:         79      16      93     327     775

  Fragments arranged by size: 

                 775     327      93      79      16
 
EagI C'GGCC_G

Cuts at:      0      79    1290 
   Size:         79    1211
 
EarI CTCTTCn'nnn_

Cuts at:      0     637     647    1290 
   Size:        637      10     643

  Fragments arranged by size: 

                 643     637      10
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     144     322     343     396     616     967    1138    1290 
   Size:        144     178      21      53     220     351     171     152

  Fragments arranged by size: 

                 351     220     178     171     152     144      53      21
            
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     973    1290 
   Size:        973     317
 
EcoO109I rG'GnC_Cy

Cuts at:      0     183     358     402     729    1092    1290 
   Size:        183     175      44     327     363     198

  Fragments arranged by size: 

                 363     327     198     183     175      44
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     972    1290 
   Size:        972     318
 
HaeII r_GCGC'y

Cuts at:      0      64      77     116    1074    1290 
   Size:         64      13      39     958     216

  Fragments arranged by size: 

                 958     216      64      39      13
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     350     382     597     629     890     916     922     948 
   Size:        350      32     215      32     261      26       6      26

Cuts at:    948    1290 
   Size:        342

  Fragments arranged by size: 

                 350     342     261     215      32      32      26      26
                   6
 
HincII GTy'rAC

Cuts at:      0     698    1290 
   Size:        698     592
 
KasI G'GCGC_C

Cuts at:      0      60      73    1290 
   Size:         60      13    1217

  Fragments arranged by size: 

                1217      60      13
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     846    1290 
   Size:        846     444
 
MscI TGG'CCA

Cuts at:      0     190     517    1290 
   Size:        190     327     773

  Fragments arranged by size: 

                 773     327     190
 
MslI CAynn'nnrTG

Cuts at:      0     838    1290 
   Size:        838     452
 
MspA1I CmG'CkG

Cuts at:      0     594    1290 
   Size:        594     696
 
NarI GG'CG_CC

Cuts at:      0      61      74    1290 
   Size:         61      13    1216

  Fragments arranged by size: 

                1216      61      13
 
NcoI C'CATG_G

Cuts at:      0     546    1290 
   Size:        546     744
 
PasI CC'CwG_GG

Cuts at:      0      45    1290 
   Size:         45    1245
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     531     915    1290 
   Size:        531     384     375

  Fragments arranged by size: 

                 531     384     375
 
PfoI T'CCnGG_A

Cuts at:      0     130     335    1290 
   Size:        130     205     955

  Fragments arranged by size: 

                 955     205     130
 
PpuMI rG'GwC_Cy

Cuts at:      0     358     402     729    1290 
   Size:        358      44     327     561

  Fragments arranged by size: 

                 561     358     327      44
 
PspOMI G'GGCC_C

Cuts at:      0     758    1290 
   Size:        758     532
 
PstI C_TGCA'G

Cuts at:      0     458     593    1290 
   Size:        458     135     697

  Fragments arranged by size: 

                 697     458     135
 
PvuII CAG'CTG

Cuts at:      0     594    1290 
   Size:        594     696
 
SanDI GG'GwC_CC

Cuts at:      0     358    1290 
   Size:        358     932
 
SapI GCTCTTCn'nnn_

Cuts at:      0     637    1290 
   Size:        637     653
 
ScaI AGT'ACT

Cuts at:      0     808    1290 
   Size:        808     482
 
SfcI C'TryA_G

Cuts at:      0     454     589     997    1290 
   Size:        454     135     408     293

  Fragments arranged by size: 

                 454     408     293     135
 
SfoI GGC'GCC

Cuts at:      0      62      75    1290 
   Size:         62      13    1215

  Fragments arranged by size: 

                1215      62      13
 
SmaI CCC'GGG

Cuts at:      0    1057    1290 
   Size:       1057     233
 
SmlI C'TyrA_G

Cuts at:      0     616    1033    1290 
   Size:        616     417     257

  Fragments arranged by size: 

                 616     417     257
 
StuI AGG'CCT

Cuts at:      0     668    1290 
   Size:        668     622
 
StyI C'CwwG_G

Cuts at:      0     546     914    1184    1290 
   Size:        546     368     270     106

  Fragments arranged by size: 

                 546     368     270     106
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     322    1290 
   Size:        322     968
 
TatI w'GTAC_w

Cuts at:      0     806    1290 
   Size:        806     484
 
XmaI C'CCGG_G

Cuts at:      0    1055    1290 
   Size:       1055     235
 

 Enzymes that do cut:

     AcuI     AgeI     AhdI     AleI     AlfI    AlwNI     ApaI     AvaI
    BamHI     BanI    BanII     BbeI    BbvCI    BglII Bme1580I     BpmI
   Bpu10I    BpuEI     BsaI    BsaHI    BsaWI    BsaXI    BseRI    BseYI
     BsgI    BsiEI  BsiHKAI    BsmBI Bsp1286I    BspHI    BsrBI    BsrFI
   BstEII    BstXI    BstYI   Bsu36I     BtgI    CspCI     DrdI     EaeI
     EagI     EarI  Eco57MI    EcoNI EcoO109I     FalI    HaeII    Hin4I
   HincII     KasI     MmeI     MscI     MslI   MspA1I     NarI     NcoI
     PasI    PflMI     PfoI    PpuMI   PspOMI     PstI    PvuII    SanDI
     SapI     ScaI     SfcI     SfoI     SmaI     SmlI     StuI     StyI
    TaqII     TatI     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AloI    ApaLI     ApoI     AscI     AseI    AsiSI    AvrII     BaeI
     BbsI     BcgI    BciVI     BclI    BfrBI     BglI     BlpI    BmgBI
     BmrI     BmtI     BplI    BsaAI    BsaBI    BsiWI     BsmI    BspEI
    BspMI    BsrDI    BsrGI   BssHII    BssSI   BstAPI    BstBI  BstZ17I
    BtgZI     BtsI     ClaI     DraI   DraIII     EciI  EcoICRI    EcoRI
    EcoRV     FseI     FspI    FspAI  HindIII     HpaI     KpnI     MfeI
     MluI     NaeI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI
     NspI     PacI     PciI     PmeI     PmlI     PpiI    PshAI     PsiI
    PspXI     PsrI     PvuI    RsrII     SacI    SacII     SalI     SbfI
    SexAI     SfiI     SgfI    SgrAI    SnaBI     SpeI     SphI     SrfI
     SspI     SwaI     TstI  Tth111I     XbaI     XcmI     XhoI     XmnI
     ZraI
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