Restriction map for pF1KE1065
##################################
# NO CUT ENZYMES (   90 enzymes) #
##################################

AatII       Acc65I      AccIII      AclI        AflII       
AgeI        AhdI        AocI        Aor51HI     AscI        
AseI        AsnI        Asp718I     AspEI       AspI        
AvrII       BbvCI       BciVI       BfaI        BfrI        
BlnI        BlpI        Bpu1102I    BseAI       BsiWI       
Bsp1407I    BspEI       BspLU11I    BsrDI       BsrGI       
BssHII      Bst1107I    BstBI       BstEII      BstPI       
BstZ17I     Bsu36I      CelII       CpoI        DraI        
DrdI        Eam1105I    Eco47III    Eco81I      EcoO65I     
EcoRI       EcoRV       EcoT22I     FseI        HindIII     
HpaI        KpnI        MaeI        MfeI        MluI        
MroI        MseI        MunI        NdeI        NheI        
NotI        NruI        NsiI        NspV        PacI        
PaeR7I      PflFI       PinAI       PmeI        PpuMI       
PshBI       Psp1406I    PvuI        RsrII       SapI        
ScaI        SexAI       SfiI        SfuI        SgrAI       
SnaBI       SpeI        SphI        SspBI       SspI        
SwaI        Tru9I       Tth111I     XbaI        XhoI        


###################################
# ONE CUT ENZYMES (   35 enzymes) #
###################################

AccI           94  GTCGAC               GT^[AC][GT]AC
AcsI         2804  AAATTT               [GA]^AATT[CT]
Alw44I       2221  GTGCAC               G^TGCAC
ApaLI        2221  GTGCAC               G^TGCAC
ApoI         2804  AAATTT               [GA]^AATT[CT]
Asp700I      2862  GAACTACTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BalI         2304  TGGCCA               TGG^CCA
BclI         2825  TGATCA               T^GATCA
BsaBI        2505  GATCCTCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI         758  ACCGGA               [AT]^CCGG[AT]
BseRI        1587  CTCCTC               GAGGAG(10/8)
BsmI         2467  GAATGC               GAATGC(1/-1)
BspDI         136  ATCGAT               AT^CGAT
BspHI        2567  TCATGA               T^CATGA
ClaI          136  ATCGAT               AT^CGAT
DraIII       1989  CACACCGTG            CAC[ACGT][ACGT][ACGT]^GTG
EcoNI        2621  CCTACGAGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FbaI         2825  TGATCA               T^GATCA
KspI         1047  CCGCGG               CCGC^GG
MamI         2505  GATCCTCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI        2304  TGGCCA               TGG^CCA
MscI         2304  TGGCCA               TGG^CCA
NaeI         1769  GCCGGC               GCC^GGC
NgoMIV       1769  GCCGGC               G^CCGGC
PflMI        1962  CCAGGACATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI         355  GACGAGTGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI         2567  TCATGA               T^CATGA
SacI         1924  GAGCTC               GAGCT^C
SacII        1047  CCGCGG               CCGC^GG
SalI           94  GTCGAC               G^TCGAC
SbfI         1731  CCTGCAGG             CCTGCA^GG
SmlI         1576  CTCAAG               C^T[CT][GA]AG
Sse8387I     1731  CCTGCAGG             CCTGCA^GG
Van91I       1962  CCAGGACATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmnI         2862  GAACTACTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   27 enzymes) #
###################################

AflIII        673  ACGTGT               A^C[GA][CT]GT
             2036  ACGTGT              
ApaI         1247  GGGCCC               GGGCC^C
             1417  GGGCCC              
AviII         733  TGCGCA               TGC^GCA
             1754  TGCGCA              
BamHI        2504  GGATCC               G^GATCC
             2666  GGATCC              
BbeI         1601  GGCGCC               GGCGC^C
             2774  GGCGCC              
BbrPI         672  CACGTG               CAC^GTG
              717  CACGTG              
BbsI          217  GAAGAC               GAAGAC(2/6)
              831  GAAGAC              
BglII         389  AGATCT               A^GATCT
             2525  AGATCT              
BpmI         2063  CTCCAG               CTGGAG(16/14)
             1837  CTGGAG              
BpuAI         217  GAAGAC               GAAGAC(2/6)
              831  GAAGAC              
BsgI          603  CTGCAC               GTGCAG(16/14)
             1095  GTGCAG              
BsmBI         535  GAGACG               CGTCTC(1/5)
              727  GAGACG              
BssSI         265  CACGAG               CACGAG(-5/-1)
             1514  CACGAG              
BstXI        1829  CCAAGGCGCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1911  CCACTTCGGTGG        
DraII         666  AGGCCCC              [GA]G^G[ACGT]CC[CT]
             2831  AGGCCCT             
EcoO109I      666  AGGCCCC              [GA]G^G[ACGT]CC[CT]
             2831  AGGCCCT             
FspI          733  TGCGCA               TGC^GCA
             1754  TGCGCA              
KasI         1601  GGCGCC               G^GCGCC
             2774  GGCGCC              
NarI         1601  GGCGCC               GG^CGCC
             2774  GGCGCC              
NcoI          404  CCATGG               C^CATGG
              510  CCATGG              
PmaCI         672  CACGTG               CAC^GTG
              717  CACGTG              
PmlI          672  CACGTG               CAC^GTG
              717  CACGTG              
SfoI         1601  GGCGCC               GGC^GCC
             2774  GGCGCC              
StuI         1679  AGGCCT               AGG^CCT
             2102  AGGCCT              
TfiI          809  GATTC                G^A[AT]TC
             2887  GAATC               
Tsp509I       619  AATT                 ^AATT
             2805  AATT                
XcmI          542  CCAAAGGTGGGGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
             2292  CCAGCCCGAGTTTGG     


#########################################
# 3 OR MORE CUT ENZYMES (  106 enzymes) #
#########################################

AccII       AciI        AcyI        AfaI        AluI        
AlwI        AlwNI       AspHI       AvaI        AvaII       
BanI        BanII       BbvI        BcgI        BcnI        
BglI        BmyI        BsaAI       BsaHI       BsaI        
BsaJI       BsiEI       BsiHKAI     BsiYI       BslI        
BsmAI       BsmFI       BsoBI       Bsp1286I    BspMI       
BsrBI       BsrFI       BsrI        BssKI       BstAPI      
BstF5I      BstNI       BstUI       BstYI       Cac8I       
CfoI        Cfr10I      Cfr13I      DdeI        DpnII       
DsaI        EaeI        EagI        EarI        EclXI       
Eco52I      EcoRII      EcoT14I     FauI        Fnu4HI      
FokI        HaeII       HaeIII      HapII       HgaI        
HhaI        Hin1I       HinP1I      HincII      HindII      
HinfI       HpaII       HphI        ItaI        Ksp632I     
MaeII       MaeIII      MboI        MboII       MflI        
MnlI        MslI        MspA1I      MspI        MvaI        
MvnI        MwoI        NciI        NdeII       NlaIII      
NlaIV       NspI        PleI        PstI        PvuII       
RsaI        Sau3AI      Sau96I      ScrFI       SfaNI       
SfcI        SmaI        StyI        TaqI        TseI        
Tsp45I      TspRI       TthHB8I     XhoII       XmaCI       
XmaI        
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com