(Circular) (Six-base) MAPSORT of: pF1KE1065.seq Check: 2657 from: 1 to: 6077 pF1KE1065 6077 bp With 182 enzymes: SgfI * February 26, 2009 15:00 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 1075 1075 Size: 6077 AccI GT'mk_AC Cuts at: 203 3115 203 Size: 2912 3165 Acc65I G'GTAC_C Cuts at: 3093 3093 Size: 6077 AclI AA'CG_TT Cuts at: 5917 5917 Size: 6077 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 3902 4334 5081 5349 3902 Size: 432 747 268 4630 Fragments arranged by size: 4630 747 432 268 AflIII A'CryG_T Cuts at: 781 2144 3429 4533 5510 781 Size: 1363 1285 1104 977 1348 Fragments arranged by size: 1363 1348 1285 1104 977 AgeI A'CCGG_T Cuts at: 4465 4465 Size: 6077 AleI CACnn'nnGTG Cuts at: 755 892 2024 755 Size: 137 1132 4808 Fragments arranged by size: 4808 1132 137 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1507 1507 Size: 6077 AlwNI CAG_nnn'CTG Cuts at: 889 1146 1650 1839 1909 3382 4949 889 Size: 257 504 189 70 1473 1567 2017 Fragments arranged by size: 2017 1567 1473 504 257 189 70 ApaI G_GGCC'C Cuts at: 1359 1529 1359 Size: 170 5907 ApaLI G'TGCA_C Cuts at: 2329 4847 2329 Size: 2518 3559 ApoI r'AATT_y Cuts at: 2912 3081 5430 5686 2912 Size: 169 2349 256 3303 Fragments arranged by size: 3303 2349 256 169 AseI AT'TA_AT Cuts at: 20 20 Size: 6077 AsiSI GCG_AT'CGC Cuts at: 104 104 Size: 6077 AvaI C'yCGr_G Cuts at: 390 1128 1275 1352 1522 2242 2404 3097 390 Size: 738 147 77 170 720 162 693 3370 Fragments arranged by size: 3370 738 720 693 170 162 147 77 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 390 423 390 Size: 33 6044 BamHI G'GATC_C Cuts at: 2612 2774 3102 2612 Size: 162 328 5587 Fragments arranged by size: 5587 328 162 BanI G'GyrC_C Cuts at: 1507 1709 2014 2134 2296 2392 2882 3093 3507 Size: 202 305 120 162 96 490 211 414 Cuts at: 3507 3755 3790 1507 Size: 248 35 3794 Fragments arranged by size: 3794 490 414 305 248 211 202 162 120 96 35 BanII G_rGCy'C Cuts at: 191 1359 1529 2036 2243 3091 4121 191 Size: 1168 170 507 207 848 1030 2147 Fragments arranged by size: 2147 1168 1030 848 507 207 170 BbeI G_GCGC'C Cuts at: 1713 2886 3511 3759 1713 Size: 1173 625 248 4031 Fragments arranged by size: 4031 1173 625 248 BbsI GAAGACnn'nnnn_ Cuts at: 332 946 332 Size: 614 5463 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 813 847 887 921 1854 1888 2004 2038 2439 Size: 34 40 34 933 34 116 34 401 Cuts at: 2439 2473 813 Size: 34 4417 Fragments arranged by size: 4417 933 401 116 40 34 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3975 4736 6077 3975 Size: 761 1341 3975 Fragments arranged by size: 3975 1341 761 BclI T'GATC_A Cuts at: 2933 3597 2933 Size: 664 5413 BglI GCCn_nnn'nGGC Cuts at: 542 1037 2222 2390 542 Size: 495 1185 168 4229 Fragments arranged by size: 4229 1185 495 168 BglII A'GATC_T Cuts at: 497 2633 3592 5629 497 Size: 2136 959 2037 945 Fragments arranged by size: 2136 2037 959 945 BlpI GC'TnA_GC Cuts at: 3189 3189 Size: 6077 Bme1580I G_kGCm'C Cuts at: 160 229 630 1359 1529 2107 2139 2227 2333 Size: 69 401 729 170 578 32 88 106 Cuts at: 2333 2825 3702 3795 4851 160 Size: 492 877 93 1056 1386 Fragments arranged by size: 1386 1056 877 729 578 492 401 170 106 93 88 69 32 BmgBI CAC'GTC Cuts at: 207 839 2699 2952 207 Size: 632 1860 253 3332 Fragments arranged by size: 3332 1860 632 253 BmrI ACTGGGnnnn_n' Cuts at: 3289 3370 3705 5849 3289 Size: 81 335 2144 3517 Fragments arranged by size: 3517 2144 335 81 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2708 4444 2708 Size: 1736 4341 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1966 2156 1966 Size: 190 5887 Bpu10I CC'TnA_GC Cuts at: 1456 2237 1456 Size: 781 5296 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 1669 3253 4132 4624 4922 1669 Size: 1584 879 492 298 2824 Fragments arranged by size: 2824 1584 879 492 298 BsaI GGTCTCn'nnnn_ Cuts at: 1627 1905 2710 5781 1627 Size: 278 805 3071 1923 Fragments arranged by size: 3071 1923 805 278 BsaAI yAC'GTr Cuts at: 782 827 2228 3430 782 Size: 45 1401 1202 3429 Fragments arranged by size: 3429 1401 1202 45 BsaBI GATnn'nnATC Cuts at: 6 2617 3591 5628 6 Size: 2611 974 2037 455 Fragments arranged by size: 2611 2037 974 455 BsaHI Gr'CG_yC Cuts at: 646 1710 2883 3508 3756 4458 5954 646 Size: 1064 1173 625 248 702 1496 769 Fragments arranged by size: 1496 1173 1064 769 702 625 248 BsaWI w'CCGG_w Cuts at: 866 3266 3787 4465 4739 4886 5297 866 Size: 2400 521 678 274 147 411 1646 Fragments arranged by size: 2400 1646 678 521 411 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1072 1102 1072 Size: 30 6047 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1686 1686 Size: 6077 BseYI C'CCAG_C Cuts at: 1358 1528 2107 2562 3502 4260 4837 5333 1358 Size: 170 579 455 940 758 577 496 2102 Fragments arranged by size: 2102 940 758 579 577 496 455 170 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 696 1224 696 Size: 528 5549 BsiEI CG_ry'CG Cuts at: 104 122 822 867 1878 2189 3619 3665 4873 Size: 18 700 45 1011 311 1430 46 1208 Cuts at: 4873 104 Size: 1308 Fragments arranged by size: 1430 1308 1208 1011 700 311 46 45 18 BsiHKAI G_wGCw'C Cuts at: 220 1090 2036 2333 3091 3869 4059 4851 220 Size: 870 946 297 758 778 190 792 1446 Fragments arranged by size: 1446 946 870 792 778 758 297 190 BsmI GAATG_Cn' Cuts at: 2581 2581 Size: 6077 BsmBI CGTCTCn'nnnn_ Cuts at: 637 829 637 Size: 192 5885 Bsp1286I G_dGCh'C Cuts at: 160 191 220 229 630 1090 1359 1529 2036 Size: 31 29 9 401 460 269 170 507 Cuts at: 2036 2107 2139 2227 2243 2333 2825 3091 3702 Size: 71 32 88 16 90 492 266 611 Cuts at: 3702 3795 3869 4059 4121 4851 160 Size: 93 74 190 62 730 1386 Fragments arranged by size: 1386 730 611 507 492 460 401 269 266 190 170 93 90 88 74 71 62 32 31 29 16 9 BspEI T'CCGG_A Cuts at: 5297 5297 Size: 6077 BspHI T'CATG_A Cuts at: 2675 5253 2675 Size: 2578 3499 BspMI ACCTGCnnnn'nnnn_ Cuts at: 418 580 1075 2728 3127 3341 3643 4024 418 Size: 162 495 1653 399 214 302 381 2471 Fragments arranged by size: 2471 1653 495 399 381 302 214 162 BsrBI CCG'CTC Cuts at: 50 584 1098 2759 4369 4423 5907 6072 50 Size: 534 514 1661 1610 54 1484 165 55 Fragments arranged by size: 1661 1610 1484 534 514 165 55 54 BsrDI GCAATG_nn' Cuts at: 3989 3989 Size: 6077 BsrFI r'CCGG_y Cuts at: 1877 2251 2524 4075 4256 4465 1877 Size: 374 273 1551 181 209 3489 Fragments arranged by size: 3489 1551 374 273 209 181 BssSI C'ACGA_G Cuts at: 373 1622 4348 4706 373 Size: 1249 2726 358 1744 Fragments arranged by size: 2726 1744 1249 358 BstAPI GCAn_nnn'nTGC Cuts at: 1650 2102 2255 2381 3454 1650 Size: 452 153 126 1073 4273 Fragments arranged by size: 4273 1073 452 153 126 BstBI TT'CG_AA Cuts at: 4438 4438 Size: 6077 BstXI CCAn_nnnn'nTGG Cuts at: 1944 2026 1944 Size: 82 5995 BstYI r'GATC_y Cuts at: 1 497 1580 2612 2633 2774 3102 3569 3592 Size: 496 1083 1032 21 141 328 467 23 Cuts at: 3592 3927 4173 5185 5271 5283 5629 1 Size: 335 246 1012 86 12 346 449 Fragments arranged by size: 1083 1032 1012 496 467 449 346 335 328 246 141 86 23 21 12 BtgI C'CryG_G Cuts at: 107 512 618 1007 1155 1713 2219 3013 107 Size: 405 106 389 148 558 506 794 3171 Fragments arranged by size: 3171 794 558 506 405 389 148 106 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 912 2574 2678 2727 2891 4027 4208 4371 912 Size: 1662 104 49 164 1136 181 163 2618 Fragments arranged by size: 2618 1662 1136 181 164 163 104 49 BtsI GCAGTG_nn' Cuts at: 325 922 1182 1659 2331 2421 2481 2496 3320 Size: 597 260 477 672 90 60 15 824 Cuts at: 3320 325 Size: 3082 Fragments arranged by size: 3082 824 672 597 477 260 90 60 15 ClaI AT'CG_AT Cuts at: 245 245 Size: 6077 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 465 500 465 Size: 35 6042 DraI TTT'AAA Cuts at: 3076 3076 Size: 6077 DraIII CAC_nnn'GTG Cuts at: 2102 2102 Size: 6077 DrdI GACnn_nn'nnGTC Cuts at: 3783 4641 3783 Size: 858 5219 EaeI y'GGCC_r Cuts at: 119 173 1716 1875 2037 2186 2343 2412 3662 Size: 54 1543 159 162 149 157 69 1250 Cuts at: 3662 3836 4227 4254 5599 119 Size: 174 391 27 1345 597 Fragments arranged by size: 1543 1345 1250 597 391 174 162 159 157 149 69 54 27 EagI C'GGCC_G Cuts at: 119 1875 2186 3662 119 Size: 1756 311 1476 2534 Fragments arranged by size: 2534 1756 1476 311 EarI CTCTTCn'nnn_ Cuts at: 1429 1699 1778 2677 2950 4100 4310 1429 Size: 270 79 899 273 1150 210 3196 Fragments arranged by size: 3196 1150 899 273 270 210 79 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 4285 4595 4741 5887 4285 Size: 310 146 1146 4475 Fragments arranged by size: 4475 1146 310 146 EcoICRI GAG'CTC Cuts at: 2034 3089 2034 Size: 1055 5022 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1966 2156 3902 4334 5081 5349 1966 Size: 190 1746 432 747 268 2694 Fragments arranged by size: 2694 1746 747 432 268 190 EcoNI CCTnn'n_nnAGG Cuts at: 2733 2733 Size: 6077 EcoO109I rG'GnC_Cy Cuts at: 775 2940 3216 775 Size: 2165 276 3636 Fragments arranged by size: 3636 2165 276 EcoRI G'AATT_C Cuts at: 3081 3081 Size: 6077 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 1590 2961 4096 1590 Size: 1371 1135 3571 Fragments arranged by size: 3571 1371 1135 FspI TGC'GCA Cuts at: 843 1864 3858 843 Size: 1021 1994 3062 Fragments arranged by size: 3062 1994 1021 HaeII r_GCGC'y Cuts at: 1492 1713 1945 2886 3511 3759 4781 5760 1492 Size: 221 232 941 625 248 1022 979 1809 Fragments arranged by size: 1809 1022 979 941 625 248 232 221 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 202 234 1072 1104 3919 3949 3951 3981 202 Size: 32 838 32 2815 30 2 30 2298 Fragments arranged by size: 2815 2298 838 32 32 30 30 2 HincII GTy'rAC Cuts at: 204 972 1242 3116 204 Size: 768 270 1874 3165 Fragments arranged by size: 3165 1874 768 270 KasI G'GCGC_C Cuts at: 1709 2882 3507 3755 1709 Size: 1173 625 248 4031 Fragments arranged by size: 4031 1173 625 248 KpnI G_GTAC'C Cuts at: 3097 3097 Size: 6077 MluI A'CGCG_T Cuts at: 5510 5510 Size: 6077 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 894 2129 4748 4932 894 Size: 1235 2619 184 2039 Fragments arranged by size: 2619 2039 1235 184 MscI TGG'CCA Cuts at: 2414 3838 2414 Size: 1424 4653 MslI CAynn'nnrTG Cuts at: 245 623 755 892 1664 2024 4066 4193 245 Size: 378 132 137 772 360 2042 127 2129 Fragments arranged by size: 2129 2042 772 378 360 137 132 127 MspA1I CmG'CkG Cuts at: 185 755 905 1157 1362 1532 1650 2372 2381 Size: 570 150 252 205 170 118 722 9 Cuts at: 2381 2804 3188 3502 3862 4875 5120 185 Size: 423 384 314 360 1013 245 1142 Fragments arranged by size: 1142 1013 722 570 423 384 360 314 252 245 205 170 150 118 9 NaeI GCC'GGC Cuts at: 1879 4258 1879 Size: 2379 3698 NarI GG'CG_CC Cuts at: 1710 2883 3508 3756 1710 Size: 1173 625 248 4031 Fragments arranged by size: 4031 1173 625 248 NcoI C'CATG_G Cuts at: 107 512 618 107 Size: 405 106 5566 Fragments arranged by size: 5566 405 106 NgoMIV G'CCGG_C Cuts at: 1877 4256 1877 Size: 2379 3698 NspI r_CATG'y Cuts at: 875 947 1802 1964 3130 3630 4537 875 Size: 72 855 162 1166 500 907 2415 Fragments arranged by size: 2415 1166 907 855 500 162 72 PasI CC'CwG_GG Cuts at: 153 193 153 Size: 40 6037 PciI A'CATG_T Cuts at: 4533 4533 Size: 6077 PflMI CCAn_nnn'nTGG Cuts at: 2076 2076 Size: 6077 PfoI T'CCnGG_A Cuts at: 2309 2309 Size: 6077 PmeI GTTT'AAAC Cuts at: 3076 3076 Size: 6077 PmlI CAC'GTG Cuts at: 782 827 782 Size: 45 6032 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5216 5248 5216 Size: 32 6045 PshAI GACnn'nnGTC Cuts at: 467 467 Size: 6077 PspOMI G'GGCC_C Cuts at: 1355 1525 1355 Size: 170 5907 PstI C_TGCA'G Cuts at: 676 929 1372 1496 1844 3124 3809 676 Size: 253 443 124 348 1280 685 2944 Fragments arranged by size: 2944 1280 685 443 348 253 124 PvuI CG_AT'CG Cuts at: 104 104 Size: 6077 PvuII CAG'CTG Cuts at: 755 905 1362 1532 3502 3862 755 Size: 150 457 170 1970 360 2970 Fragments arranged by size: 2970 1970 457 360 170 150 RsrII CG'GwC_CG Cuts at: 4272 4272 Size: 6077 SacI G_AGCT'C Cuts at: 2036 3091 2036 Size: 1055 5022 SacII CC_GC'GG Cuts at: 1158 1158 Size: 6077 SalI G'TCGA_C Cuts at: 202 3114 202 Size: 2912 3165 SapI GCTCTTCn'nnn_ Cuts at: 4100 4310 4100 Size: 210 5867 SbfI CC_TGCA'GG Cuts at: 1844 3124 1844 Size: 1280 4797 ScaI AGT'ACT Cuts at: 5594 5594 Size: 6077 SfcI C'TryA_G Cuts at: 33 399 672 925 1368 1492 1840 1981 3120 Size: 366 273 253 443 124 348 141 1139 Cuts at: 3120 3805 4798 4989 33 Size: 685 993 191 1121 Fragments arranged by size: 1139 1121 993 685 443 366 348 273 253 191 141 124 SfoI GGC'GCC Cuts at: 1711 2884 3509 3757 1711 Size: 1173 625 248 4031 Fragments arranged by size: 4031 1173 625 248 SgfI GCG_AT'CGC Cuts at: 104 104 Size: 6077 SmaI CCC'GGG Cuts at: 392 1130 1277 1354 2244 3099 392 Size: 738 147 77 890 855 3370 Fragments arranged by size: 3370 890 855 738 147 77 SmlI C'TyrA_G Cuts at: 1684 3232 4147 4639 4901 1684 Size: 1548 915 492 262 2860 Fragments arranged by size: 2860 1548 915 492 262 SpeI A'CTAG_T Cuts at: 67 67 Size: 6077 SphI G_CATG'C Cuts at: 3130 3630 3130 Size: 500 5577 SrfI GCCC'GGGC Cuts at: 2244 2244 Size: 6077 StuI AGG'CCT Cuts at: 1789 2212 1789 Size: 423 5654 StyI C'CwwG_G Cuts at: 107 512 618 1247 1733 1937 2999 3211 3565 Size: 405 106 629 486 204 1062 212 354 Cuts at: 3565 107 Size: 2619 Fragments arranged by size: 2619 1062 629 486 405 354 212 204 106 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 2032 2380 3660 3793 4252 4461 2032 Size: 348 1280 133 459 209 3648 Fragments arranged by size: 3648 1280 459 348 209 133 TatI w'GTAC_w Cuts at: 2981 5592 2981 Size: 2611 3466 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5594 5626 5594 Size: 32 6045 Tth111I GACn'n_nGTC Cuts at: 3874 3874 Size: 6077 XbaI T'CTAG_A Cuts at: 3108 3108 Size: 6077 XcmI CCAnnnn_n'nnnnTGG Cuts at: 657 2407 3572 657 Size: 1750 1165 3162 Fragments arranged by size: 3162 1750 1165 XmaI C'CCGG_G Cuts at: 390 1128 1275 1352 2242 3097 390 Size: 738 147 77 890 855 3370 Fragments arranged by size: 3370 890 855 738 147 77 XmnI GAAnn'nnTTC Cuts at: 2974 2974 Size: 6077 Enzymes that do cut: AarI AccI Acc65I AclI AcuI AflIII AgeI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PshAI PspOMI PstI PvuI PvuII RsrII SacI SacII SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI SrfI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI Enzymes that do not cut: AatII AfeI AflII AhdI AloI AscI AvrII BbvCI BfrBI BmtI BsiWI BsrGI BssHII BstEII BstZ17I Bsu36I EcoRV FseI FspAI HindIII HpaI MfeI NdeI NheI NotI NruI NsiI PacI PpuMI PsiI PspXI PsrI SanDI SexAI SfiI SgrAI SnaBI SspI SwaI XhoI ZraI