##################################
# NO CUT ENZYMES (  216 enzymes) #
##################################

AatII       Acc65I      AccII       AccIII      AciI        
AclI        AcsI        AcyI        AfaI        AflII       
AflIII      AgeI        AhdI        Alw44I      AlwI        
AlwNI       AocI        Aor51HI     ApaI        ApaLI       
ApoI        AscI        AseI        AsnI        Asp700I     
Asp718I     AspEI       AspHI       AspI        AvaI        
AviII       AvrII       BalI        BamHI       BanI        
BanII       BbeI        BbrPI       BbvCI       BcgI        
BciVI       BclI        BcnI        BfaI        BfrI        
BglI        BglII       BlnI        BlpI        BmyI        
BpmI        Bpu1102I    BsaAI       BsaBI       BsaHI       
BsaJI       BsaWI       BseAI       BseRI       BsiHKAI     
BsiWI       BsiYI       BslI        BsmBI       BsmFI       
BsmI        BsoBI       Bsp1286I    Bsp1407I    BspDI       
BspEI       BspHI       BspLU11I    BspMI       BsrBI       
BsrDI       BsrFI       BsrGI       BssHII      BssKI       
BssSI       Bst1107I    BstAPI      BstBI       BstEII      
BstF5I      BstNI       BstPI       BstUI       BstXI       
BstYI       BstZ17I     Bsu36I      CelII       Cfr10I      
ClaI        CpoI        DpnII       DraI        DraII       
DraIII      DrdI        DsaI        EaeI        EagI        
Eam1105I    EclXI       Eco47III    Eco52I      Eco81I      
EcoNI       EcoO109I    EcoO65I     EcoRI       EcoRII      
EcoRV       EcoT14I     EcoT22I     FauI        FbaI        
FokI        FseI        FspI        HaeIII      HapII       
Hin1I       HindIII     HinfI       HpaII       HphI        
KasI        KpnI        KspI        MaeI        MaeIII      
MamI        MboI        MfeI        MflI        MluI        
MluNI       MroI        MscI        MspA1I      MspI        
MunI        MvaI        MvnI        NaeI        NarI        
NciI        NcoI        NdeI        NdeII       NgoMIV      
NheI        NlaIII      NlaIV       NotI        NruI        
NsiI        NspI        NspV        PacI        PaeR7I      
PflFI       PflMI       PinAI       PleI        PmaCI       
PmeI        PmlI        PpuMI       PshAI       PshBI       
Psp1406I    PvuI        PvuII       RcaI        RsaI        
RsrII       SacI        SacII       SalI        SapI        
Sau3AI      SbfI        ScaI        ScrFI       SexAI       
SfaNI       SfiI        SfoI        SfuI        SgrAI       
SmaI        SmlI        SnaBI       SpeI        SphI        
Sse8387I    SspBI       StuI        StyI        SwaI        
TfiI        Tsp45I      Tth111I     Van91I      XbaI        
XcmI        XhoI        XhoII       XmaCI       XmaI        
XmnI        


###################################
# ONE CUT ENZYMES (   26 enzymes) #
###################################

AccI            3  GTCTAC               GT^[AC][GT]AC
AluI          125  AGCT                 AG^CT
AvaII          10  GGTCC                G^G[AT]CC
BbsI           38  GAAGAC               GAAGAC(2/6)
BpuAI          38  GAAGAC               GAAGAC(2/6)
BsaI          116  GAGACC               GGTCTC(1/5)
BsgI          146  GTGCAG               GTGCAG(16/14)
BsiEI         141  CGACCG               CG[GA][CT]^CG
BsrI           25  ACTGG                ACTGG(1/-1)
Cfr13I         10  GGTCC                G^G[ACGT]CC
DdeI          131  CTGAG                C^T[ACGT]AG
HaeII         166  GGCGCT               [GA]GCGC^[CT]
HgaI          151  GACGC                GACGC(5/10)
HincII         91  GTTAAC               GT[CT]^[GA]AC
HindII         91  GTTAAC               GT[CT]^[GA]AC
HpaI           91  GTTAAC               GTT^AAC
MaeII          95  ACGT                 A^CGT
MseI           92  TTAA                 T^TAA
MslI          276  CATCATCGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MwoI          154  GCACTGCAGGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
PstI          157  CTGCAG               CTGCA^G
Sau96I         10  GGTCC                G^G[ACGT]CC
SspI          209  AATATT               AAT^ATT
TaqI           36  TCGA                 T^CGA
Tru9I          92  TTAA                 T^TAA
TthHB8I        36  TCGA                 T^CGA


###################################
# TWO CUT ENZYMES (   15 enzymes) #
###################################

BbvI          190  GCAGC                GCAGC(8/12)
               19  GCTGC               
BsmAI         116  GAGAC                GTCTC(1/5)
              292  GTCTC               
Cac8I         159  GCAGGC               GC[ACGT]^[ACGT]GC
              163  GCAGGC              
CfoI          167  GCGC                 GCG^C
              188  GCGC                
EarI          294  CTCTTC               CTCTTC(1/4)
              201  GAAGAG              
Fnu4HI         19  GCTGC                GC^[ACGT]GC
              190  GCAGC               
HhaI          167  GCGC                 GCG^C
              188  GCGC                
HinP1I        167  GCGC                 G^CGC
              188  GCGC                
ItaI           19  GCTGC                GC^[ACGT]GC
              190  GCAGC               
Ksp632I       294  CTCTTC               CTCTTC(1/4)
              201  GAAGAG              
MnlI           70  GAGG                 CCTC(7/6)
              204  GAGG                
SfcI            5  CTACAG               C^T[GA][CT]AG
              157  CTGCAG              
TseI           19  GCTGC                G^C[AT]GC
              190  GCAGC               
Tsp509I       176  AATT                 ^AATT
              223  AATT                
TspRI          24  CACTG                CA[GC]TG[ACGT][ACGT]^
              155  CACTG               


#########################################
# 3 OR MORE CUT ENZYMES (    1 enzymes) #
#########################################

MboII