Restriction map for
pF1KE1064
(Circular) (Six-base) MAPSORT of: pF1KE1064.seq Check: 3857 from: 1 to: 3413
pF1KE1064 3413 bp
With 182 enzymes: SgfI *
February 26, 2009 15:00 ..
AccI GT'mk_AC
Cuts at: 112 451 112
Size: 339 3074
Acc65I G'GTAC_C
Cuts at: 429 429
Size: 3413
AclI AA'CG_TT
Cuts at: 3253 3253
Size: 3413
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 136 389 1238 1670 2417 2685 136
Size: 253 849 432 747 268 864
Fragments arranged by size:
864 849 747 432 268 253
AflIII A'CryG_T
Cuts at: 765 1869 2846 765
Size: 1104 977 1332
Fragments arranged by size:
1332 1104 977
AgeI A'CCGG_T
Cuts at: 1801 1801
Size: 3413
AlwNI CAG_nnn'CTG
Cuts at: 718 2285 718
Size: 1567 1846
ApaLI G'TGCA_C
Cuts at: 2183 2183
Size: 3413
ApoI r'AATT_y
Cuts at: 417 2766 3022 417
Size: 2349 256 808
Fragments arranged by size:
2349 808 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3413
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3413
AvaI C'yCGr_G
Cuts at: 433 433
Size: 3413
BamHI G'GATC_C
Cuts at: 438 438
Size: 3413
BanI G'GyrC_C
Cuts at: 429 843 1091 1126 429
Size: 414 248 35 2716
Fragments arranged by size:
2716 414 248 35
BanII G_rGCy'C
Cuts at: 427 1457 427
Size: 1030 2383
BbeI G_GCGC'C
Cuts at: 847 1095 847
Size: 248 3165
BbsI GAAGACnn'nnnn_
Cuts at: 153 153
Size: 3413
BciVI GTATCCnnnnn_n'
Cuts at: 1311 2072 3413 1311
Size: 761 1341 1311
Fragments arranged by size:
1341 1311 761
BclI T'GATC_A
Cuts at: 933 933
Size: 3413
BglII A'GATC_T
Cuts at: 928 2965 928
Size: 2037 1376
BlpI GC'TnA_GC
Cuts at: 525 525
Size: 3413
Bme1580I G_kGCm'C
Cuts at: 1038 1131 2187 1038
Size: 93 1056 2264
Fragments arranged by size:
2264 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 625 706 1041 3185 625
Size: 81 335 2144 853
Fragments arranged by size:
2144 853 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1780 1780
Size: 3413
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 589 1468 1960 2258 589
Size: 879 492 298 1744
Fragments arranged by size:
1744 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 218 3117 218
Size: 2899 514
BsaAI yAC'GTr
Cuts at: 766 766
Size: 3413
BsaBI GATnn'nnATC
Cuts at: 6 927 2964 6
Size: 921 2037 455
Fragments arranged by size:
2037 921 455
BsaHI Gr'CG_yC
Cuts at: 844 1092 1794 3290 844
Size: 248 702 1496 967
Fragments arranged by size:
1496 967 702 248
BsaWI w'CCGG_w
Cuts at: 602 1123 1801 2075 2222 2633 602
Size: 521 678 274 147 411 1382
Fragments arranged by size:
1382 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 838 1596 2173 2669 838
Size: 758 577 496 1582
Fragments arranged by size:
1582 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 275 275
Size: 3413
BsiEI CG_ry'CG
Cuts at: 104 252 955 1001 2209 104
Size: 148 703 46 1208 1308
Fragments arranged by size:
1308 1208 703 148 46
BsiHKAI G_wGCw'C
Cuts at: 427 1205 1395 2187 427
Size: 778 190 792 1653
Fragments arranged by size:
1653 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 427 1038 1131 1205 1395 1457 2187 427
Size: 611 93 74 190 62 730 1653
Fragments arranged by size:
1653 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 2633 2633
Size: 3413
BspHI T'CATG_A
Cuts at: 2589 2589
Size: 3413
BspMI ACCTGCnnnn'nnnn_
Cuts at: 463 677 979 1360 463
Size: 214 302 381 2516
Fragments arranged by size:
2516 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1705 1759 3243 3408 50
Size: 1655 54 1484 165 55
Fragments arranged by size:
1655 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1325 1325
Size: 3413
BsrFI r'CCGG_y
Cuts at: 1411 1592 1801 1411
Size: 181 209 3023
Fragments arranged by size:
3023 209 181
BssSI C'ACGA_G
Cuts at: 1684 2042 1684
Size: 358 3055
BstAPI GCAn_nnn'nTGC
Cuts at: 790 790
Size: 3413
BstBI TT'CG_AA
Cuts at: 1774 1774
Size: 3413
BstYI r'GATC_y
Cuts at: 1 438 905 928 1263 1509 2521 2607 2619
Size: 437 467 23 335 246 1012 86 12
Cuts at: 2619 2965 1
Size: 346 449
Fragments arranged by size:
1012 467 449 437 346 335 246 86
23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1363 1544 1707 1363
Size: 181 163 3069
Fragments arranged by size:
3069 181 163
BtsI GCAGTG_nn'
Cuts at: 262 656 262
Size: 394 3019
DraI TTT'AAA
Cuts at: 412 412
Size: 3413
DrdI GACnn_nn'nnGTC
Cuts at: 1119 1977 1119
Size: 858 2555
EaeI y'GGCC_r
Cuts at: 998 1172 1563 1590 2935 998
Size: 174 391 27 1345 1476
Fragments arranged by size:
1476 1345 391 174 27
EagI C'GGCC_G
Cuts at: 998 998
Size: 3413
EarI CTCTTCn'nnn_
Cuts at: 304 408 1436 1646 304
Size: 104 1028 210 2071
Fragments arranged by size:
2071 1028 210 104
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1621 1931 2077 3223 1621
Size: 310 146 1146 1811
Fragments arranged by size:
1811 1146 310 146
EcoICRI GAG'CTC
Cuts at: 425 425
Size: 3413
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 136 389 1238 1670 2417 2685 136
Size: 253 849 432 747 268 864
Fragments arranged by size:
864 849 747 432 268 253
EcoO109I rG'GnC_Cy
Cuts at: 552 552
Size: 3413
EcoRI G'AATT_C
Cuts at: 417 417
Size: 3413
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1432 1432
Size: 3413
FspI TGC'GCA
Cuts at: 1194 1194
Size: 3413
HaeII r_GCGC'y
Cuts at: 278 847 1095 2117 3096 278
Size: 569 248 1022 979 595
Fragments arranged by size:
1022 979 595 569 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 150 182 1255 1285 1287 1317 150
Size: 32 1073 30 2 30 2246
Fragments arranged by size:
2246 1073 32 30 30 2
HincII GTy'rAC
Cuts at: 201 452 201
Size: 251 3162
HpaI GTT'AAC
Cuts at: 201 201
Size: 3413
KasI G'GCGC_C
Cuts at: 843 1091 843
Size: 248 3165
KpnI G_GTAC'C
Cuts at: 433 433
Size: 3413
MluI A'CGCG_T
Cuts at: 2846 2846
Size: 3413
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 145 2084 2268 145
Size: 1939 184 1290
Fragments arranged by size:
1939 1290 184
MscI TGG'CCA
Cuts at: 1174 1174
Size: 3413
MslI CAynn'nnrTG
Cuts at: 388 1402 1529 388
Size: 1014 127 2272
Fragments arranged by size:
2272 1014 127
MspA1I CmG'CkG
Cuts at: 524 838 1198 2211 2456 524
Size: 314 360 1013 245 1481
Fragments arranged by size:
1481 1013 360 314 245
NaeI GCC'GGC
Cuts at: 1594 1594
Size: 3413
NarI GG'CG_CC
Cuts at: 844 1092 844
Size: 248 3165
NgoMIV G'CCGG_C
Cuts at: 1592 1592
Size: 3413
NspI r_CATG'y
Cuts at: 466 966 1873 466
Size: 500 907 2006
Fragments arranged by size:
2006 907 500
PciI A'CATG_T
Cuts at: 1869 1869
Size: 3413
PmeI GTTT'AAAC
Cuts at: 412 412
Size: 3413
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2552 2584 2552
Size: 32 3381
PstI C_TGCA'G
Cuts at: 269 460 1145 269
Size: 191 685 2537
Fragments arranged by size:
2537 685 191
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3413
PvuII CAG'CTG
Cuts at: 838 1198 838
Size: 360 3053
RsrII CG'GwC_CG
Cuts at: 1608 1608
Size: 3413
SacI G_AGCT'C
Cuts at: 427 427
Size: 3413
SalI G'TCGA_C
Cuts at: 450 450
Size: 3413
SapI GCTCTTCn'nnn_
Cuts at: 1436 1646 1436
Size: 210 3203
SbfI CC_TGCA'GG
Cuts at: 460 460
Size: 3413
ScaI AGT'ACT
Cuts at: 2930 2930
Size: 3413
SfcI C'TryA_G
Cuts at: 33 113 265 456 1141 2134 2325 33
Size: 80 152 191 685 993 191 1121
Fragments arranged by size:
1121 993 685 191 191 152 80
SfoI GGC'GCC
Cuts at: 845 1093 845
Size: 248 3165
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3413
SmaI CCC'GGG
Cuts at: 435 435
Size: 3413
SmlI C'TyrA_G
Cuts at: 568 1483 1975 2237 568
Size: 915 492 262 1744
Fragments arranged by size:
1744 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3413
SphI G_CATG'C
Cuts at: 466 966 466
Size: 500 2913
SspI AAT'ATT
Cuts at: 319 319
Size: 3413
StyI C'CwwG_G
Cuts at: 547 901 547
Size: 354 3059
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 996 1129 1588 1797 996
Size: 133 459 209 2612
Fragments arranged by size:
2612 459 209 133
TatI w'GTAC_w
Cuts at: 2928 2928
Size: 3413
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2930 2962 2930
Size: 32 3381
Tth111I GACn'n_nGTC
Cuts at: 1210 1210
Size: 3413
XbaI T'CTAG_A
Cuts at: 444 444
Size: 3413
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 908 908
Size: 3413
XmaI C'CCGG_G
Cuts at: 433 433
Size: 3413
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BciVI BclI BglII BlpI Bme1580I BmrI BplI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseYI BsgI
BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstYI BtgZI BtsI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HpaI KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NgoMIV NspI PciI PmeI PpiI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI SspI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbvCI BcgI BfrBI BglI
BmgBI BmtI BpmI Bpu10I BsaXI BseRI BsiWI BsmI
BsmBI BsrGI BssHII BstEII BstXI BstZ17I Bsu36I BtgI
ClaI CspCI DraIII EcoNI EcoRV FseI FspAI HindIII
MfeI NcoI NdeI NheI NotI NruI NsiI PacI
PasI PflMI PfoI PmlI PpuMI PshAI PsiI PspOMI
PspXI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI
SrfI StuI SwaI XhoI XmnI ZraI