(Circular) (Six-base) MAPSORT of: pF1KE1062.seq Check: 9189 from: 1 to: 7475 pF1KE1062 7475 bp With 182 enzymes: SgfI * January 15, 2009 16:32 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 1000 1560 2145 1000 Size: 560 585 6330 Fragments arranged by size: 6330 585 560 AccI GT'mk_AC Cuts at: 3760 4513 3760 Size: 753 6722 Acc65I G'GTAC_C Cuts at: 4491 4491 Size: 7475 AclI AA'CG_TT Cuts at: 253 7315 253 Size: 7062 413 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 764 908 1159 1469 2405 3272 4088 4367 5300 Size: 144 251 310 936 867 816 279 933 Cuts at: 5300 5732 6479 6747 764 Size: 432 747 268 1492 Fragments arranged by size: 1492 936 933 867 816 747 432 310 279 268 251 144 AfeI AGC'GCT Cuts at: 2542 2542 Size: 7475 AflIII A'CryG_T Cuts at: 1623 4827 5931 6908 1623 Size: 3204 1104 977 2190 Fragments arranged by size: 3204 2190 1104 977 AgeI A'CCGG_T Cuts at: 5863 5863 Size: 7475 AhdI GACnn_n'nnGTC Cuts at: 2337 2921 3756 2337 Size: 584 835 6056 Fragments arranged by size: 6056 835 584 AleI CACnn'nnGTG Cuts at: 3436 3436 Size: 7475 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 466 2153 466 Size: 1687 5788 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 243 275 243 Size: 32 7443 AlwNI CAG_nnn'CTG Cuts at: 835 1858 2085 3289 4780 6347 835 Size: 1023 227 1204 1491 1567 1963 Fragments arranged by size: 1963 1567 1491 1204 1023 227 ApaLI G'TGCA_C Cuts at: 6245 6245 Size: 7475 ApoI r'AATT_y Cuts at: 1132 1871 2103 2899 3546 3723 4479 6828 7084 Size: 739 232 796 647 177 756 2349 256 Cuts at: 7084 1132 Size: 1523 Fragments arranged by size: 2349 1523 796 756 739 647 256 232 177 AseI AT'TA_AT Cuts at: 20 1955 20 Size: 1935 5540 AsiSI GCG_AT'CGC Cuts at: 104 104 Size: 7475 AvaI C'yCGr_G Cuts at: 178 1493 3927 4495 178 Size: 1315 2434 568 3158 Fragments arranged by size: 3158 2434 1315 568 AvrII C'CTAG_G Cuts at: 3318 3318 Size: 7475 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 3166 3199 3166 Size: 33 7442 BamHI G'GATC_C Cuts at: 2565 3322 3578 4500 2565 Size: 757 256 922 5540 Fragments arranged by size: 5540 922 757 256 BanI G'GyrC_C Cuts at: 182 189 196 4491 4905 5153 5188 182 Size: 7 7 4295 414 248 35 2469 Fragments arranged by size: 4295 2469 414 248 35 7 7 BanII G_rGCy'C Cuts at: 129 1377 3373 4489 5519 129 Size: 1248 1996 1116 1030 2085 Fragments arranged by size: 2085 1996 1248 1116 1030 BbeI G_GCGC'C Cuts at: 186 193 200 4909 5157 186 Size: 7 7 4709 248 2504 Fragments arranged by size: 4709 2504 248 7 7 BbsI GAAGACnn'nnnn_ Cuts at: 550 4120 4285 4382 550 Size: 3570 165 97 3643 Fragments arranged by size: 3643 3570 165 97 BbvCI CC'TCA_GC Cuts at: 139 139 Size: 7475 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 2732 2766 3491 3525 2732 Size: 34 725 34 6682 Fragments arranged by size: 6682 725 34 34 BciVI GTATCCnnnnn_n' Cuts at: 282 5373 6134 7475 282 Size: 5091 761 1341 282 Fragments arranged by size: 5091 1341 761 282 BclI T'GATC_A Cuts at: 4053 4995 4053 Size: 942 6533 BglI GCCn_nnn'nGGC Cuts at: 112 112 Size: 7475 BglII A'GATC_T Cuts at: 3570 4990 7027 3570 Size: 1420 2037 4018 Fragments arranged by size: 4018 2037 1420 BlpI GC'TnA_GC Cuts at: 4587 4587 Size: 7475 Bme1580I G_kGCm'C Cuts at: 3843 4344 5100 5193 6249 3843 Size: 501 756 93 1056 5069 Fragments arranged by size: 5069 1056 756 501 93 BmgBI CAC'GTC Cuts at: 1541 1541 Size: 7475 BmrI ACTGGGnnnn_n' Cuts at: 2060 3141 4331 4687 4768 5103 7247 2060 Size: 1081 1190 356 81 335 2144 2288 Fragments arranged by size: 2288 2144 1190 1081 356 335 81 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 1760 2459 4331 5842 1760 Size: 699 1872 1511 3393 Fragments arranged by size: 3393 1872 1511 699 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1042 1391 2039 2126 2432 3311 3551 3605 3616 Size: 349 648 87 306 879 240 54 11 Cuts at: 3616 3870 3887 4178 1042 Size: 254 17 291 4339 Fragments arranged by size: 4339 879 648 349 306 291 254 240 87 54 17 11 Bpu10I CC'TnA_GC Cuts at: 139 1728 139 Size: 1589 5886 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 1085 3653 4651 5530 6022 6320 1085 Size: 2568 998 879 492 298 2240 Fragments arranged by size: 2568 2240 998 879 492 298 BsaI GGTCTCn'nnnn_ Cuts at: 1521 2212 3780 4351 7179 1521 Size: 691 1568 571 2828 1817 Fragments arranged by size: 2828 1817 1568 691 571 BsaAI yAC'GTr Cuts at: 1841 2406 2738 4828 1841 Size: 565 332 2090 4488 Fragments arranged by size: 4488 2090 565 332 BsaBI GATnn'nnATC Cuts at: 6 4989 7026 6 Size: 4983 2037 455 Fragments arranged by size: 4983 2037 455 BsaHI Gr'CG_yC Cuts at: 183 190 197 408 3425 4906 5154 5856 7352 Size: 7 7 211 3017 1481 248 702 1496 Cuts at: 7352 183 Size: 306 Fragments arranged by size: 3017 1496 1481 702 306 248 211 7 7 BsaWI w'CCGG_w Cuts at: 4664 5185 5863 6137 6284 6695 4664 Size: 521 678 274 147 411 5444 Fragments arranged by size: 5444 678 521 411 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 243 273 1404 1434 3633 3663 243 Size: 30 1131 30 2199 30 4055 Fragments arranged by size: 4055 2199 1131 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 127 979 1481 2066 2435 3371 4074 127 Size: 852 502 585 369 936 703 3528 Fragments arranged by size: 3528 936 852 703 585 502 369 BseYI C'CCAG_C Cuts at: 601 1051 1788 2569 2817 3815 3824 4006 4244 Size: 450 737 781 248 998 9 182 238 Cuts at: 4244 4900 5658 6235 6731 601 Size: 656 758 577 496 1345 Fragments arranged by size: 1345 998 781 758 737 656 577 496 450 248 238 182 9 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 4032 4372 4032 Size: 340 7135 BsiEI CG_ry'CG Cuts at: 104 206 1688 5017 5063 6271 104 Size: 102 1482 3329 46 1208 1308 Fragments arranged by size: 3329 1482 1308 1208 102 46 BsiHKAI G_wGCw'C Cuts at: 4489 5267 5457 6249 4489 Size: 778 190 792 5715 Fragments arranged by size: 5715 792 778 190 BsiWI C'GTAC_G Cuts at: 2406 2406 Size: 7475 BsmI GAATG_Cn' Cuts at: 245 453 725 2844 3120 245 Size: 208 272 2119 276 4600 Fragments arranged by size: 4600 2119 276 272 208 BsmBI CGTCTCn'nnnn_ Cuts at: 4040 4040 Size: 7475 Bsp1286I G_dGCh'C Cuts at: 129 1377 3373 3843 4344 4489 5100 5193 5267 Size: 1248 1996 470 501 145 611 93 74 Cuts at: 5267 5457 5519 6249 129 Size: 190 62 730 1355 Fragments arranged by size: 1996 1355 1248 730 611 501 470 190 145 93 74 62 BspEI T'CCGG_A Cuts at: 6695 6695 Size: 7475 BspHI T'CATG_A Cuts at: 2718 3585 3638 6651 2718 Size: 867 53 3013 3542 Fragments arranged by size: 3542 3013 867 53 BspMI ACCTGCnnnn'nnnn_ Cuts at: 544 1000 1560 2145 4525 4739 5041 5422 544 Size: 456 560 585 2380 214 302 381 2597 Fragments arranged by size: 2597 2380 585 560 456 381 302 214 BsrBI CCG'CTC Cuts at: 50 120 215 2415 3347 5767 5821 7305 7470 Size: 70 95 2200 932 2420 54 1484 165 Cuts at: 7470 50 Size: 55 Fragments arranged by size: 2420 2200 1484 932 165 95 70 55 54 BsrDI GCAATG_nn' Cuts at: 450 741 1337 1869 4219 5387 450 Size: 291 596 532 2350 1168 2538 Fragments arranged by size: 2538 2350 1168 596 532 291 BsrFI r'CCGG_y Cuts at: 282 3305 4310 5473 5654 5863 282 Size: 3023 1005 1163 181 209 1894 Fragments arranged by size: 3023 1894 1163 1005 209 181 BsrGI T'GTAC_A Cuts at: 1691 1691 Size: 7475 BssSI C'ACGA_G Cuts at: 2375 5746 6104 2375 Size: 3371 358 3746 Fragments arranged by size: 3746 3371 358 BstAPI GCAn_nnn'nTGC Cuts at: 4852 4852 Size: 7475 BstBI TT'CG_AA Cuts at: 249 2046 3996 5836 249 Size: 1797 1950 1840 1888 Fragments arranged by size: 1950 1888 1840 1797 BstEII G'GTnAC_C Cuts at: 2913 2913 Size: 7475 BstXI CCAn_nnnn'nTGG Cuts at: 2526 3441 3575 4419 2526 Size: 915 134 844 5582 Fragments arranged by size: 5582 915 844 134 BstYI r'GATC_y Cuts at: 1 427 831 1560 2565 2667 3322 3570 3578 Size: 426 404 729 1005 102 655 248 8 Cuts at: 3578 4500 4967 4990 5325 5571 6583 6669 6681 Size: 922 467 23 335 246 1012 86 12 Cuts at: 6681 7027 1 Size: 346 449 Fragments arranged by size: 1012 1005 922 729 655 467 449 426 404 346 335 248 246 102 86 23 12 8 Bsu36I CC'TnA_GG Cuts at: 836 1769 3282 836 Size: 933 1513 5029 Fragments arranged by size: 5029 1513 933 BtgI C'CryG_G Cuts at: 107 200 2955 3695 3937 107 Size: 93 2755 740 242 3645 Fragments arranged by size: 3645 2755 740 242 93 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 5425 5606 5769 5425 Size: 181 163 7131 Fragments arranged by size: 7131 181 163 BtsI GCAGTG_nn' Cuts at: 1477 1747 4133 4718 1477 Size: 270 2386 585 4234 Fragments arranged by size: 4234 2386 585 270 DraI TTT'AAA Cuts at: 386 2879 4474 386 Size: 2493 1595 3387 Fragments arranged by size: 3387 2493 1595 DraIII CAC_nnn'GTG Cuts at: 2583 2661 4275 2583 Size: 78 1614 5783 Fragments arranged by size: 5783 1614 78 DrdI GACnn_nn'nnGTC Cuts at: 2241 5181 6039 2241 Size: 2940 858 3677 Fragments arranged by size: 3677 2940 858 EaeI y'GGCC_r Cuts at: 203 497 4094 5060 5234 5625 5652 6997 203 Size: 294 3597 966 174 391 27 1345 681 Fragments arranged by size: 3597 1345 966 681 391 294 174 27 EagI C'GGCC_G Cuts at: 203 5060 203 Size: 4857 2618 EarI CTCTTCn'nnn_ Cuts at: 315 2405 4035 4159 5498 5708 315 Size: 2090 1630 124 1339 210 2082 Fragments arranged by size: 2090 2082 1630 1339 210 124 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 130 1560 5683 5993 6139 7285 130 Size: 1430 4123 310 146 1146 320 Fragments arranged by size: 4123 1430 1146 320 310 146 EcoICRI GAG'CTC Cuts at: 4487 4487 Size: 7475 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 764 908 1042 1159 1391 1469 2039 2126 2405 Size: 144 134 117 232 78 570 87 279 Cuts at: 2405 2432 3272 3311 3551 3605 3616 3870 3887 Size: 27 840 39 240 54 11 254 17 Cuts at: 3887 4088 4178 4367 5300 5732 6479 6747 764 Size: 201 90 189 933 432 747 268 1492 Fragments arranged by size: 1492 933 840 747 570 432 279 268 254 240 232 201 189 144 134 117 90 87 78 54 39 27 17 11 EcoNI CCTnn'n_nnAGG Cuts at: 476 476 Size: 7475 EcoO109I rG'GnC_Cy Cuts at: 3241 4062 4198 4446 4614 3241 Size: 821 136 248 168 6102 Fragments arranged by size: 6102 821 248 168 136 EcoRI G'AATT_C Cuts at: 1132 2899 3546 4479 1132 Size: 1767 647 933 4128 Fragments arranged by size: 4128 1767 933 647 EcoRV GAT'ATC Cuts at: 845 3675 845 Size: 2830 4645 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 1126 1961 5494 1126 Size: 835 3533 3107 Fragments arranged by size: 3533 3107 835 FspI TGC'GCA Cuts at: 5256 5256 Size: 7475 HaeII r_GCGC'y Cuts at: 186 193 200 2544 4909 5157 6179 7158 186 Size: 7 7 2344 2365 248 1022 979 503 Fragments arranged by size: 2365 2344 1022 979 503 248 7 7 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 280 312 829 861 2244 2276 2323 2355 2557 Size: 32 517 32 1383 32 47 32 202 Cuts at: 2557 2589 2637 2669 2907 2939 3307 3339 3633 Size: 32 48 32 238 32 368 32 294 Cuts at: 3633 3665 3742 3774 5317 5347 5349 5379 280 Size: 32 77 32 1543 30 2 30 2376 Fragments arranged by size: 2376 1543 1383 517 368 294 238 202 77 48 47 32 32 32 32 32 32 32 32 32 32 30 30 2 HincII GTy'rAC Cuts at: 3185 3687 4514 3185 Size: 502 827 6146 Fragments arranged by size: 6146 827 502 HpaI GTT'AAC Cuts at: 3185 3185 Size: 7475 KasI G'GCGC_C Cuts at: 182 189 196 4905 5153 182 Size: 7 7 4709 248 2504 Fragments arranged by size: 4709 2504 248 7 7 KpnI G_GTAC'C Cuts at: 4495 4495 Size: 7475 MfeI C'AATT_G Cuts at: 1091 1091 Size: 7475 MluI A'CGCG_T Cuts at: 6908 6908 Size: 7475 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 964 1240 1335 3004 3350 4352 6146 6330 964 Size: 276 95 1669 346 1002 1794 184 2109 Fragments arranged by size: 2109 1794 1669 1002 346 276 184 95 MscI TGG'CCA Cuts at: 499 4096 5236 499 Size: 3597 1140 2738 Fragments arranged by size: 3597 2738 1140 MslI CAynn'nnrTG Cuts at: 2431 2524 2677 3040 3436 3439 3955 4366 5464 Size: 93 153 363 396 3 516 411 1098 Cuts at: 5464 5591 2431 Size: 127 4315 Fragments arranged by size: 4315 1098 516 411 396 363 153 127 93 3 MspA1I CmG'CkG Cuts at: 202 2241 2250 4586 4900 5260 6273 6518 202 Size: 2039 9 2336 314 360 1013 245 1159 Fragments arranged by size: 2336 2039 1159 1013 360 314 245 9 NaeI GCC'GGC Cuts at: 3307 4312 5656 3307 Size: 1005 1344 5126 Fragments arranged by size: 5126 1344 1005 NarI GG'CG_CC Cuts at: 183 190 197 4906 5154 183 Size: 7 7 4709 248 2504 Fragments arranged by size: 4709 2504 248 7 7 NcoI C'CATG_G Cuts at: 107 2955 3695 3937 107 Size: 2848 740 242 3645 Fragments arranged by size: 3645 2848 740 242 NdeI CA'TA_TG Cuts at: 1809 1809 Size: 7475 NgoMIV G'CCGG_C Cuts at: 3305 4310 5654 3305 Size: 1005 1344 5126 Fragments arranged by size: 5126 1344 1005 NotI GC'GGCC_GC Cuts at: 203 203 Size: 7475 NspI r_CATG'y Cuts at: 3759 4046 4528 5028 5935 3759 Size: 287 482 500 907 5299 Fragments arranged by size: 5299 907 500 482 287 PciI A'CATG_T Cuts at: 5931 5931 Size: 7475 PflMI CCAn_nnn'nTGG Cuts at: 950 1858 950 Size: 908 6567 PfoI T'CCnGG_A Cuts at: 419 2016 4194 419 Size: 1597 2178 3700 Fragments arranged by size: 3700 2178 1597 PmeI GTTT'AAAC Cuts at: 4474 4474 Size: 7475 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 243 275 6614 6646 243 Size: 32 6339 32 1072 Fragments arranged by size: 6339 1072 32 32 PpuMI rG'GwC_Cy Cuts at: 3241 4062 4198 4446 3241 Size: 821 136 248 6270 Fragments arranged by size: 6270 821 248 136 PshAI GACnn'nnGTC Cuts at: 3246 3246 Size: 7475 PsiI TTA'TAA Cuts at: 1883 1883 Size: 7475 PstI C_TGCA'G Cuts at: 4522 5207 4522 Size: 685 6790 PvuI CG_AT'CG Cuts at: 104 104 Size: 7475 PvuII CAG'CTG Cuts at: 2241 2250 4900 5260 2241 Size: 9 2650 360 4456 Fragments arranged by size: 4456 2650 360 9 RsrII CG'GwC_CG Cuts at: 5670 5670 Size: 7475 SacI G_AGCT'C Cuts at: 4489 4489 Size: 7475 SacII CC_GC'GG Cuts at: 203 203 Size: 7475 SalI G'TCGA_C Cuts at: 4512 4512 Size: 7475 SanDI GG'GwC_CC Cuts at: 3241 3241 Size: 7475 SapI GCTCTTCn'nnn_ Cuts at: 315 4159 5498 5708 315 Size: 3844 1339 210 2082 Fragments arranged by size: 3844 2082 1339 210 SbfI CC_TGCA'GG Cuts at: 4522 4522 Size: 7475 ScaI AGT'ACT Cuts at: 6992 6992 Size: 7475 SfcI C'TryA_G Cuts at: 33 4518 5203 6196 6387 33 Size: 4485 685 993 191 1121 Fragments arranged by size: 4485 1121 993 685 191 SfoI GGC'GCC Cuts at: 184 191 198 4907 5155 184 Size: 7 7 4709 248 2504 Fragments arranged by size: 4709 2504 248 7 7 SgfI GCG_AT'CGC Cuts at: 104 104 Size: 7475 SmaI CCC'GGG Cuts at: 3929 4497 3929 Size: 568 6907 SmlI C'TyrA_G Cuts at: 1100 3668 4630 5545 6037 6299 1100 Size: 2568 962 915 492 262 2276 Fragments arranged by size: 2568 2276 962 915 492 262 SpeI A'CTAG_T Cuts at: 67 67 Size: 7475 SphI G_CATG'C Cuts at: 4528 5028 4528 Size: 500 6975 StuI AGG'CCT Cuts at: 2194 2652 2194 Size: 458 7017 StyI C'CwwG_G Cuts at: 107 1601 2090 2222 2955 2993 3318 3695 3937 Size: 1494 489 132 733 38 325 377 242 Cuts at: 3937 4609 4963 107 Size: 672 354 2619 Fragments arranged by size: 2619 1494 733 672 489 377 354 325 242 132 38 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 1658 2602 3006 4172 5058 5191 5650 5859 1658 Size: 944 404 1166 886 133 459 209 3274 Fragments arranged by size: 3274 1166 944 886 459 404 209 133 TatI w'GTAC_w Cuts at: 1691 1889 2831 2935 3103 3536 6990 1691 Size: 198 942 104 168 433 3454 2176 Fragments arranged by size: 3454 2176 942 433 198 168 104 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4197 4229 6992 7024 4197 Size: 32 2763 32 4648 Fragments arranged by size: 4648 2763 32 32 Tth111I GACn'n_nGTC Cuts at: 5272 5272 Size: 7475 XbaI T'CTAG_A Cuts at: 4506 4506 Size: 7475 XcmI CCAnnnn_n'nnnnTGG Cuts at: 359 656 947 1223 2559 3381 4368 4970 359 Size: 297 291 276 1336 822 987 602 2864 Fragments arranged by size: 2864 1336 987 822 602 297 291 276 XmaI C'CCGG_G Cuts at: 3927 4495 3927 Size: 568 6907 XmnI GAAnn'nnTTC Cuts at: 2975 3992 4168 2975 Size: 1017 176 6282 Fragments arranged by size: 6282 1017 176 Enzymes that do cut: AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BaeI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NotI NspI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII RsrII SacI SacII SalI SanDI SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI Enzymes that do not cut: AatII AflII ApaI AscI BfrBI BmtI BssHII BstZ17I ClaI CspCI FseI FspAI HindIII NheI NruI NsiI PacI PasI PmlI PspOMI PspXI PsrI SexAI SfiI SgrAI SnaBI SrfI SspI SwaI XhoI ZraI