Restriction map for
pF1KE0532
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# NO CUT ENZYMES ( 174 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcsI AfaI AflII AgeI AhdI
AlwNI AocI Aor51HI ApaI ApoI
AscI AseI AsnI Asp700I Asp718I
AspEI AspI AvaI AviII AvrII
BamHI BbeI BbrPI BbsI BbvCI
BciVI BclI BcnI BfaI BfrI
BglI BglII BlnI BlpI BpmI
Bpu1102I BpuAI BsaAI BsaBI BsaI
BsaWI BseAI BseRI BsgI BsiEI
BsiWI BsmAI BsmBI BsoBI Bsp1407I
BspDI BspEI BspHI BspMI BsrDI
BsrFI BsrGI BssHII BssSI Bst1107I
BstBI BstEII BstPI BstXI BstYI
BstZ17I Bsu36I CelII Cfr10I ClaI
DdeI DraI DraII DraIII DrdI
EagI Eam1105I EarI EclXI Eco47III
Eco52I Eco81I EcoNI EcoO109I EcoO65I
EcoRI EcoRV EcoT22I FbaI FseI
FspI HapII HincII HindII HindIII
HinfI HpaI HpaII HphI KasI
KpnI Ksp632I MaeI MaeII MamI
MfeI MflI MluI MroI MspI
MunI NaeI NarI NciI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PflMI PinAI PleI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PstI PvuI RcaI RsaI SalI
SapI SbfI ScaI SexAI SfcI
SfiI SfoI SfuI SgrAI SmaI
SmlI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI SwaI TfiI
Tth111I Van91I XbaI XcmI XhoI
XhoII XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 42 enzymes) #
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AcyI 91 GGCGTC G[GA]^CG[CT]C
AflIII 381 ACATGT A^C[GA][CT]GT
Alw44I 217 GTGCAC G^TGCAC
AlwI 457 GATCC GGATC(4/5)
ApaLI 217 GTGCAC G^TGCAC
AvaII 23 GGTCC G^G[AT]CC
BalI 450 TGGCCA TGG^CCA
BanI 204 GGTGCC G^G[CT][GA]CC
BanII 178 GAGCTC G[GA]GC[CT]^C
BcgI 209 CGAACACTGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BsaHI 91 GGCGTC G[GA]^CG[CT]C
BsmI 285 GAATGC GAATGC(1/-1)
BspLU11I 381 ACATGT A^CATGT
BsrBI 87 GAGCGG CCGCTC(-3/-3)
BsrI 345 ACTGG ACTGG(1/-1)
BstAPI 28 GCACGCGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstF5I 64 GGATG GGATG(2/0)
Cfr13I 23 GGTCC G^G[ACGT]CC
CpoI 22 CGGTCCG CG^G[AT]CCG
DsaI 19 CCGCGG C^C[GA][CT]GG
EaeI 450 TGGCCA [CT]^GGCC[GA]
EcoT14I 415 CCAAGG C^C[AT][AT]GG
FokI 64 GGATG GGATG(9/13)
HaeII 411 AGCGCC [GA]GCGC^[CT]
HgaI 92 GCGTC GACGC(5/10)
Hin1I 91 GGCGTC G[GA]^CG[CT]C
KspI 19 CCGCGG CCGC^GG
MaeIII 295 GTCAC ^GT[ACGT]AC
MluNI 450 TGGCCA TGG^CCA
MscI 450 TGGCCA TGG^CCA
NlaIV 204 GGTGCC GG[ACGT]^[ACGT]CC
NspI 381 ACATGT [GA]CATG^[CT]
PvuII 229 CAGCTG CAG^CTG
RsrII 22 CGGTCCG CG^G[AT]CCG
SacI 178 GAGCTC GAGCT^C
SacII 19 CCGCGG CCGC^GG
Sau96I 23 GGTCC G^G[ACGT]CC
SfaNI 65 GATGC GCATC(5/9)
StyI 415 CCAAGG C^C[AT][AT]GG
TaqI 341 TCGA T^CGA
Tsp45I 295 GTCAC ^GT[GC]AC
TthHB8I 341 TCGA T^CGA
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# TWO CUT ENZYMES ( 18 enzymes) #
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AspHI 178 GAGCTC G[AT]GC[AT]^C
217 GTGCAC
BbvI 372 GCAGC GCAGC(8/12)
34 GCTGC
BmyI 178 GAGCTC G[AGT]GC[ACT]^C
217 GTGCAC
BsiHKAI 178 GAGCTC G[AT]GC[AT]^C
217 GTGCAC
BsmFI 146 GGGAC GGGAC(10/14)
252 GGGAC
Bsp1286I 178 GAGCTC G[AGT]GC[ACT]^C
217 GTGCAC
BssKI 191 CCTGG ^CC[ACGT]GG
305 CCAGG
BstNI 191 CCTGG CC^[AT]GG
305 CCAGG
EcoRII 191 CCTGG ^CC[AT]GG
305 CCAGG
FauI 18 CCCGC CCCGC(4/6)
82 GCGGG
MseI 329 TTAA T^TAA
359 TTAA
MslI 120 CACCTTCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
237 CATCACTGTG
MvaI 191 CCTGG CC^[AT]GG
305 CCAGG
ScrFI 191 CCTGG CC^[ACGT]GG
305 CCAGG
Tru9I 329 TTAA T^TAA
359 TTAA
TseI 34 GCTGC G^C[AT]GC
372 GCAGC
Tsp509I 224 AATT ^AATT
357 AATT
TspRI 213 CACTG CA[GC]TG[ACGT][ACGT]^
240 CACTG
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# 3 OR MORE CUT ENZYMES ( 24 enzymes) #
#########################################
AccII AciI AluI BsaJI BsiYI
BslI BstUI Cac8I CfoI DpnII
Fnu4HI HaeIII HhaI HinP1I ItaI
MboI MboII MnlI MspA1I MvnI
MwoI NdeII NlaIII Sau3AI