(Circular) (Six-base) MAPSORT of: pF1KE0532.seq Check: 7065 from: 1 to: 3575 pF1KE0532 3575 bp With 182 enzymes: SgfI * April 24, 2009 13:15 .. AccI GT'mk_AC Cuts at: 613 613 Size: 3575 Acc65I G'GTAC_C Cuts at: 591 591 Size: 3575 AclI AA'CG_TT Cuts at: 3415 3415 Size: 3575 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1400 1832 2579 2847 1400 Size: 432 747 268 2128 Fragments arranged by size: 2128 747 432 268 AflIII A'CryG_T Cuts at: 489 927 2031 3008 489 Size: 438 1104 977 1056 Fragments arranged by size: 1104 1056 977 438 AgeI A'CCGG_T Cuts at: 1963 1963 Size: 3575 AleI CACnn'nnGTG Cuts at: 232 232 Size: 3575 AlwNI CAG_nnn'CTG Cuts at: 880 2447 880 Size: 1567 2008 ApaLI G'TGCA_C Cuts at: 325 2345 325 Size: 2020 1555 ApoI r'AATT_y Cuts at: 579 2928 3184 579 Size: 2349 256 970 Fragments arranged by size: 2349 970 256 AseI AT'TA_AT Cuts at: 20 20 Size: 3575 AsiSI GCG_AT'CGC Cuts at: 104 104 Size: 3575 AvaI C'yCGr_G Cuts at: 595 595 Size: 3575 BamHI G'GATC_C Cuts at: 600 600 Size: 3575 BanI G'GyrC_C Cuts at: 312 591 1005 1253 1288 312 Size: 279 414 248 35 2599 Fragments arranged by size: 2599 414 279 248 35 BanII G_rGCy'C Cuts at: 290 589 1619 290 Size: 299 1030 2246 Fragments arranged by size: 2246 1030 299 BbeI G_GCGC'C Cuts at: 1009 1257 1009 Size: 248 3327 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 306 340 306 Size: 34 3541 BciVI GTATCCnnnnn_n' Cuts at: 1473 2234 3575 1473 Size: 761 1341 1473 Fragments arranged by size: 1473 1341 761 BclI T'GATC_A Cuts at: 1095 1095 Size: 3575 BglII A'GATC_T Cuts at: 1090 3127 1090 Size: 2037 1538 BlpI GC'TnA_GC Cuts at: 687 687 Size: 3575 Bme1580I G_kGCm'C Cuts at: 329 1200 1293 2349 329 Size: 871 93 1056 1555 Fragments arranged by size: 1555 1056 871 93 BmrI ACTGGGnnnn_n' Cuts at: 787 868 1203 3347 787 Size: 81 335 2144 1015 Fragments arranged by size: 2144 1015 335 81 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 1942 1942 Size: 3575 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 751 1630 2122 2420 751 Size: 879 492 298 1906 Fragments arranged by size: 1906 879 492 298 BsaI GGTCTCn'nnnn_ Cuts at: 3279 3279 Size: 3575 BsaAI yAC'GTr Cuts at: 928 928 Size: 3575 BsaBI GATnn'nnATC Cuts at: 6 1089 3126 6 Size: 1083 2037 455 Fragments arranged by size: 2037 1083 455 BsaHI Gr'CG_yC Cuts at: 200 1006 1254 1956 3452 200 Size: 806 248 702 1496 323 Fragments arranged by size: 1496 806 702 323 248 BsaWI w'CCGG_w Cuts at: 764 1285 1963 2237 2384 2795 764 Size: 521 678 274 147 411 1544 Fragments arranged by size: 1544 678 521 411 274 147 BseYI C'CCAG_C Cuts at: 1000 1758 2335 2831 1000 Size: 758 577 496 1744 Fragments arranged by size: 1744 758 577 496 BsiEI CG_ry'CG Cuts at: 104 1117 1163 2371 104 Size: 1013 46 1208 1308 Fragments arranged by size: 1308 1208 1013 46 BsiHKAI G_wGCw'C Cuts at: 290 329 589 1367 1557 2349 290 Size: 39 260 778 190 792 1516 Fragments arranged by size: 1516 792 778 260 190 39 BsmI GAATG_Cn' Cuts at: 399 399 Size: 3575 Bsp1286I G_dGCh'C Cuts at: 290 329 589 1200 1293 1367 1557 1619 2349 Size: 39 260 611 93 74 190 62 730 Cuts at: 2349 290 Size: 1516 Fragments arranged by size: 1516 730 611 260 190 93 74 62 39 BspEI T'CCGG_A Cuts at: 2795 2795 Size: 3575 BspHI T'CATG_A Cuts at: 2751 2751 Size: 3575 BspMI ACCTGCnnnn'nnnn_ Cuts at: 625 839 1141 1522 625 Size: 214 302 381 2678 Fragments arranged by size: 2678 381 302 214 BsrBI CCG'CTC Cuts at: 50 197 1867 1921 3405 3570 50 Size: 147 1670 54 1484 165 55 Fragments arranged by size: 1670 1484 165 147 55 54 BsrDI GCAATG_nn' Cuts at: 1487 1487 Size: 3575 BsrFI r'CCGG_y Cuts at: 1573 1754 1963 1573 Size: 181 209 3185 Fragments arranged by size: 3185 209 181 BssSI C'ACGA_G Cuts at: 1846 2204 1846 Size: 358 3217 BstAPI GCAn_nnn'nTGC Cuts at: 142 952 142 Size: 810 2765 BstBI TT'CG_AA Cuts at: 1936 1936 Size: 3575 BstYI r'GATC_y Cuts at: 1 600 1067 1090 1425 1671 2683 2769 2781 Size: 599 467 23 335 246 1012 86 12 Cuts at: 2781 3127 1 Size: 346 449 Fragments arranged by size: 1012 599 467 449 346 335 246 86 23 12 BtgI C'CryG_G Cuts at: 107 127 107 Size: 20 3555 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 1525 1706 1869 1525 Size: 181 163 3231 Fragments arranged by size: 3231 181 163 BtsI GCAGTG_nn' Cuts at: 818 818 Size: 3575 DraI TTT'AAA Cuts at: 574 574 Size: 3575 DrdI GACnn_nn'nnGTC Cuts at: 1281 2139 1281 Size: 858 2717 EaeI y'GGCC_r Cuts at: 558 1160 1334 1725 1752 3097 558 Size: 602 174 391 27 1345 1036 Fragments arranged by size: 1345 1036 602 391 174 27 EagI C'GGCC_G Cuts at: 1160 1160 Size: 3575 EarI CTCTTCn'nnn_ Cuts at: 1598 1808 1598 Size: 210 3365 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 193 1783 2093 2239 3385 193 Size: 1590 310 146 1146 383 Fragments arranged by size: 1590 1146 383 310 146 EcoICRI GAG'CTC Cuts at: 288 587 288 Size: 299 3276 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1400 1832 2579 2847 1400 Size: 432 747 268 2128 Fragments arranged by size: 2128 747 432 268 EcoO109I rG'GnC_Cy Cuts at: 714 714 Size: 3575 EcoRI G'AATT_C Cuts at: 579 579 Size: 3575 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 1594 1594 Size: 3575 FspI TGC'GCA Cuts at: 1356 1356 Size: 3575 HaeII r_GCGC'y Cuts at: 523 1009 1257 2279 3258 523 Size: 486 248 1022 979 840 Fragments arranged by size: 1022 979 840 486 248 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 526 558 1417 1447 1449 1479 526 Size: 32 859 30 2 30 2622 Fragments arranged by size: 2622 859 32 30 30 2 HincII GTy'rAC Cuts at: 614 614 Size: 3575 KasI G'GCGC_C Cuts at: 1005 1253 1005 Size: 248 3327 KpnI G_GTAC'C Cuts at: 595 595 Size: 3575 MluI A'CGCG_T Cuts at: 3008 3008 Size: 3575 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 2246 2430 2246 Size: 184 3391 MscI TGG'CCA Cuts at: 560 1336 560 Size: 776 2799 MslI CAynn'nnrTG Cuts at: 232 349 1564 1691 232 Size: 117 1215 127 2116 Fragments arranged by size: 2116 1215 127 117 MspA1I CmG'CkG Cuts at: 129 339 483 686 1000 1360 2373 2618 129 Size: 210 144 203 314 360 1013 245 1086 Fragments arranged by size: 1086 1013 360 314 245 210 203 144 NaeI GCC'GGC Cuts at: 1756 1756 Size: 3575 NarI GG'CG_CC Cuts at: 1006 1254 1006 Size: 248 3327 NcoI C'CATG_G Cuts at: 107 107 Size: 3575 NgoMIV G'CCGG_C Cuts at: 1754 1754 Size: 3575 NspI r_CATG'y Cuts at: 493 628 1128 2035 493 Size: 135 500 907 2033 Fragments arranged by size: 2033 907 500 135 PasI CC'CwG_GG Cuts at: 413 413 Size: 3575 PciI A'CATG_T Cuts at: 489 2031 489 Size: 1542 2033 PmeI GTTT'AAAC Cuts at: 574 574 Size: 3575 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2714 2746 2714 Size: 32 3543 PstI C_TGCA'G Cuts at: 622 1307 622 Size: 685 2890 PvuI CG_AT'CG Cuts at: 104 104 Size: 3575 PvuII CAG'CTG Cuts at: 339 1000 1360 339 Size: 661 360 2554 Fragments arranged by size: 2554 661 360 RsrII CG'GwC_CG Cuts at: 131 1770 131 Size: 1639 1936 SacI G_AGCT'C Cuts at: 290 589 290 Size: 299 3276 SacII CC_GC'GG Cuts at: 130 130 Size: 3575 SalI G'TCGA_C Cuts at: 612 612 Size: 3575 SapI GCTCTTCn'nnn_ Cuts at: 1598 1808 1598 Size: 210 3365 SbfI CC_TGCA'GG Cuts at: 622 622 Size: 3575 ScaI AGT'ACT Cuts at: 3092 3092 Size: 3575 SfcI C'TryA_G Cuts at: 33 618 1303 2296 2487 33 Size: 585 685 993 191 1121 Fragments arranged by size: 1121 993 685 585 191 SfoI GGC'GCC Cuts at: 1007 1255 1007 Size: 248 3327 SgfI GCG_AT'CGC Cuts at: 104 104 Size: 3575 SmaI CCC'GGG Cuts at: 597 597 Size: 3575 SmlI C'TyrA_G Cuts at: 730 1645 2137 2399 730 Size: 915 492 262 1906 Fragments arranged by size: 1906 915 492 262 SpeI A'CTAG_T Cuts at: 67 67 Size: 3575 SphI G_CATG'C Cuts at: 628 1128 628 Size: 500 3075 StyI C'CwwG_G Cuts at: 107 523 709 1063 107 Size: 416 186 354 2619 Fragments arranged by size: 2619 416 354 186 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 1158 1291 1750 1959 1158 Size: 133 459 209 2774 Fragments arranged by size: 2774 459 209 133 TatI w'GTAC_w Cuts at: 3090 3090 Size: 3575 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3092 3124 3092 Size: 32 3543 Tth111I GACn'n_nGTC Cuts at: 1372 1372 Size: 3575 XbaI T'CTAG_A Cuts at: 606 606 Size: 3575 XcmI CCAnnnn_n'nnnnTGG Cuts at: 1070 1070 Size: 3575 XmaI C'CCGG_G Cuts at: 595 595 Size: 3575 Enzymes that do cut: AccI Acc65I AclI AcuI AflIII AgeI AleI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BcgI BciVI BclI BglII BlpI Bme1580I BmrI BplI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseYI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI PasI PciI PmeI PpiI PstI PvuI PvuII RsrII SacI SacII SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI Enzymes that do not cut: AarI AatII AfeI AflII AhdI AlfI AloI ApaI AscI AvrII BaeI BbsI BbvCI BfrBI BglI BmgBI BmtI BpmI Bpu10I BsaXI BseRI BsgI BsiWI BsmBI BsrGI BssHII BstEII BstXI BstZ17I Bsu36I ClaI CspCI DraIII EcoNI EcoRV FseI FspAI HindIII HpaI MfeI NdeI NheI NotI NruI NsiI PacI PflMI PfoI PmlI PpuMI PshAI PsiI PspOMI PspXI PsrI SanDI SexAI SfiI SgrAI SnaBI SrfI SspI StuI SwaI XhoI XmnI ZraI