Restriction map for
pF1KE0405
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# NO CUT ENZYMES ( 188 enzymes) #
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AatII Acc65I AccII AccIII AclI
AcyI AgeI AhdI Alw44I AlwI
AlwNI AocI Aor51HI ApaI ApaLI
AscI AseI AsnI Asp700I Asp718I
AspEI AspHI AspI AvaI AviII
AvrII BalI BamHI BanI BanII
BbeI BbvCI BbvI BciVI BclI
BfaI BglII BlnI BlpI BmyI
Bpu1102I BsaBI BsaHI BsaI BsaWI
BseAI BseRI BsgI BsiEI BsiHKAI
BsiWI BsmAI BsmBI BsoBI Bsp1286I
Bsp1407I BspDI BspEI BspHI BspLU11I
BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII
BstPI BstUI BstXI BstYI Bsu36I
CelII CfoI Cfr10I ClaI CpoI
DdeI DpnII DraI DraII DraIII
DsaI EaeI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoNI EcoO109I
EcoO65I EcoRI EcoRV EcoT14I FauI
FbaI Fnu4HI FseI FspI HaeII
HaeIII HgaI HhaI Hin1I HinP1I
HindIII HpaI ItaI KasI KpnI
KspI MaeI MamI MboI MfeI
MflI MluI MluNI MroI MscI
MslI MspA1I MunI MvnI NaeI
NarI NcoI NdeI NdeII NgoMIV
NheI NlaIII NotI NruI NspI
NspV PacI PaeR7I PflFI PflMI
PinAI PmeI PpuMI PshAI PshBI
Psp1406I PvuI PvuII RcaI RsrII
SacI SacII SalI SapI Sau3AI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspBI StuI StyI
SwaI TseI Tsp45I TspRI Tth111I
Van91I XbaI XcmI XhoI XhoII
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 39 enzymes) #
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AciI 795 CCGC CCGC(-3/-1)
AcsI 456 AAATTT [GA]^AATT[CT]
AflII 432 CTTAAG C^TTAAG
ApoI 456 AAATTT [GA]^AATT[CT]
BbrPI 276 CACGTG CAC^GTG
BcgI 821 GCAATCCAATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BcnI 789 CCGGG CC^[GC]GG
BfrI 432 CTTAAG C^TTAAG
BglI 812 GCCTGGAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpmI 814 CTGGAG CTGGAG(16/14)
BsiYI 651 CCTCTACTTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 651 CCTCTACTTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmFI 791 GGGAC GGGAC(10/14)
BsmI 622 GCATTC GAATGC(1/-1)
Bst1107I 310 GTATAC GTA^TAC
BstZ17I 310 GTATAC GTA^TAC
Cac8I 54 GCCAGC GC[ACGT]^[ACGT]GC
DrdI 6 GACTCTGCAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 802 GAAGAG CTCTTC(1/4)
EcoT22I 620 ATGCAT ATGCA^T
HapII 789 CCGG C^CGG
HincII 325 GTTGAC GT[CT]^[GA]AC
HindII 325 GTTGAC GT[CT]^[GA]AC
HpaII 789 CCGG C^CGG
HphI 644 TCACC GGTGA(8/7)
Ksp632I 802 GAAGAG CTCTTC(1/4)
MspI 789 CCGG C^CGG
MwoI 812 GCCTGGAGGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NciI 789 CCGGG CC^[GC]GG
NlaIV 791 GGGACC GG[ACGT]^[ACGT]CC
NsiI 620 ATGCAT ATGCA^T
PmaCI 276 CACGTG CAC^GTG
PmlI 276 CACGTG CAC^GTG
PstI 10 CTGCAG CTGCA^G
SfcI 10 CTGCAG C^T[GA][CT]AG
SmlI 432 CTTAAG C^T[CT][GA]AG
SspI 166 AATATT AAT^ATT
TaqI 443 TCGA T^CGA
TthHB8I 443 TCGA T^CGA
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# TWO CUT ENZYMES ( 12 enzymes) #
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AfaI 340 GTAC GT^AC
482 GTAC
AflIII 277 ACGTGT A^C[GA][CT]GT
708 ACGTGT
AvaII 660 GGACC G^G[AT]CC
792 GGACC
BsaAI 276 CACGTG [CT]AC^GT[GA]
707 TACGTG
BsaJI 671 CCCTGG C^C[ACGT][ACGT]GG
834 CCTGGG
BsrI 547 CCAGT ACTGG(1/-1)
779 CCAGT
Cfr13I 660 GGACC G^G[ACGT]CC
792 GGACC
MaeII 277 ACGT A^CGT
708 ACGT
MaeIII 30 GTTAC ^GT[ACGT]AC
782 GTTAC
RsaI 340 GTAC GT^AC
482 GTAC
Sau96I 660 GGACC G^G[ACGT]CC
792 GGACC
SfaNI 334 GATGC GCATC(5/9)
843 GCATC
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# 3 OR MORE CUT ENZYMES ( 19 enzymes) #
#########################################
AccI AluI BbsI BpuAI BssKI
BstF5I BstNI EcoRII FokI HinfI
MboII MnlI MseI MvaI PleI
ScrFI TfiI Tru9I Tsp509I