Restriction map for pF1KE0405
 (Circular) (Six-base) MAPSORT of: pF1KE0405.seq  Check: 3920  from: 1  to: 4007



pF1KE0405  4007 bp

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:14  ..

AccI GT'mk_AC

Cuts at:    124     419     813    1045     124 
   Size:        295     394     232    3086

  Fragments arranged by size: 

                3086     394     295     232
 
Acc65I G'GTAC_C

Cuts at:   1023    1023 
   Size:       4007
 
AclI AA'CG_TT

Cuts at:   3847    3847 
   Size:       4007
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:    814    1832    2264    3011    3279     814 
   Size:       1018     432     747     268    1542

  Fragments arranged by size: 

                1542    1018     747     432     268
 
AflII C'TTAA_G

Cuts at:    540     540 
   Size:       4007
 
AflIII A'CryG_T

Cuts at:    385     816    1359    2463    3440     385 
   Size:        431     543    1104     977     952

  Fragments arranged by size: 

                1104     977     952     543     431
 
AgeI A'CCGG_T

Cuts at:   2395    2395 
   Size:       4007
 
AlwNI CAG_nnn'CTG

Cuts at:   1312    2879    1312 
   Size:       1567    2440
 
ApaLI G'TGCA_C

Cuts at:   2777    2777 
   Size:       4007
 
ApoI r'AATT_y

Cuts at:    564    1011    3360    3616     564 
   Size:        447    2349     256     955

  Fragments arranged by size: 

                2349     955     447     256
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       4007
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       4007
 
AvaI C'yCGr_G

Cuts at:   1027    1027 
   Size:       4007
 
BamHI G'GATC_C

Cuts at:   1032    1032 
   Size:       4007
 
BanI G'GyrC_C

Cuts at:   1023    1437    1685    1720    1023 
   Size:        414     248      35    3310

  Fragments arranged by size: 

                3310     414     248      35
 
BanII G_rGCy'C

Cuts at:   1021    2051    1021 
   Size:       1030    2977
 
BbeI G_GCGC'C

Cuts at:   1441    1689    1441 
   Size:        248    3759
 
BbsI GAAGACnn'nnnn_

Cuts at:    118     310     552     118 
   Size:        192     242    3573

  Fragments arranged by size: 

                3573     242     192
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:    918     952     918 
   Size:         34    3973
 
BciVI GTATCCnnnnn_n'

Cuts at:   1905    2666    4007    1905 
   Size:        761    1341    1905

  Fragments arranged by size: 

                1905    1341     761
 
BclI T'GATC_A

Cuts at:   1527    1527 
   Size:       4007
 
BfrBI ATG'CAT

Cuts at:    730     730 
   Size:       4007
 
BglI GCCn_nnn'nGGC

Cuts at:    926     926 
   Size:       4007
 
BglII A'GATC_T

Cuts at:   1522    3559    1522 
   Size:       2037    1970
 
BlpI GC'TnA_GC

Cuts at:   1119    1119 
   Size:       4007
 
Bme1580I G_kGCm'C

Cuts at:   1632    1725    2781    1632 
   Size:         93    1056    2858

  Fragments arranged by size: 

                2858    1056      93
 
BmrI ACTGGGnnnn_n'

Cuts at:   1219    1300    1635    3779    1219 
   Size:         81     335    2144    1447

  Fragments arranged by size: 

                2144    1447     335      81
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2374    2374 
   Size:       4007
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:    943     943 
   Size:       4007
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1183    2062    2554    2852    1183 
   Size:        879     492     298    2338

  Fragments arranged by size: 

                2338     879     492     298
 
BsaI GGTCTCn'nnnn_

Cuts at:   3711    3711 
   Size:       4007
 
BsaAI yAC'GTr

Cuts at:    386     817    1360     386 
   Size:        431     543    3033

  Fragments arranged by size: 

                3033     543     431
 
BsaBI GATnn'nnATC

Cuts at:      6    1521    3558       6 
   Size:       1515    2037     455

  Fragments arranged by size: 

                2037    1515     455
 
BsaHI Gr'CG_yC

Cuts at:   1438    1686    2388    3884    1438 
   Size:        248     702    1496    1561

  Fragments arranged by size: 

                1561    1496     702     248
 
BsaWI w'CCGG_w

Cuts at:   1196    1717    2395    2669    2816    3227    1196 
   Size:        521     678     274     147     411    1976

  Fragments arranged by size: 

                1976     678     521     411     274     147
 
BseYI C'CCAG_C

Cuts at:    176    1432    2190    2767    3263     176 
   Size:       1256     758     577     496     920

  Fragments arranged by size: 

                1256     920     758     577     496
 
BsiEI CG_ry'CG

Cuts at:    104    1549    1595    2803     104 
   Size:       1445      46    1208    1308

  Fragments arranged by size: 

                1445    1308    1208      46
 
BsiHKAI G_wGCw'C

Cuts at:   1021    1799    1989    2781    1021 
   Size:        778     190     792    2247

  Fragments arranged by size: 

                2247     792     778     190
 
BsmI GAATG_Cn'

Cuts at:    730     730 
   Size:       4007
 
Bsp1286I G_dGCh'C

Cuts at:   1021    1632    1725    1799    1989    2051    2781    1021 
   Size:        611      93      74     190      62     730    2247

  Fragments arranged by size: 

                2247     730     611     190      93      74      62
 
BspEI T'CCGG_A

Cuts at:   3227    3227 
   Size:       4007
 
BspHI T'CATG_A

Cuts at:   3183    3183 
   Size:       4007
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:   1057    1271    1573    1954    1057 
   Size:        214     302     381    3110

  Fragments arranged by size: 

                3110     381     302     214
 
BsrBI CCG'CTC

Cuts at:     50    2299    2353    3837    4002      50 
   Size:       2249      54    1484     165      55

  Fragments arranged by size: 

                2249    1484     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:   1919    1919 
   Size:       4007
 
BsrFI r'CCGG_y

Cuts at:   2005    2186    2395    2005 
   Size:        181     209    3617

  Fragments arranged by size: 

                3617     209     181
 
BssSI C'ACGA_G

Cuts at:   2278    2636    2278 
   Size:        358    3649
 
BstAPI GCAn_nnn'nTGC

Cuts at:   1384    1384 
   Size:       4007
 
BstBI TT'CG_AA

Cuts at:   2368    2368 
   Size:       4007
 
BstYI r'GATC_y

Cuts at:      1    1032    1499    1522    1857    2103    3115    3201    3213 
   Size:       1031     467      23     335     246    1012      86      12

Cuts at:   3213    3559       1 
   Size:        346     449

  Fragments arranged by size: 

                1031    1012     467     449     346     335     246      86
                  23      12
 
BstZ17I GTA'TAC

Cuts at:    420     420 
   Size:       4007
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:   1957    2138    2301    1957 
   Size:        181     163    3663

  Fragments arranged by size: 

                3663     181     163
 
BtsI GCAGTG_nn'

Cuts at:   1250    1250 
   Size:       4007
 
DraI TTT'AAA

Cuts at:   1006    1006 
   Size:       4007
 
DrdI GACnn_nn'nnGTC

Cuts at:    120    1713    2571     120 
   Size:       1593     858    1556

  Fragments arranged by size: 

                1593    1556     858
 
EaeI y'GGCC_r

Cuts at:   1592    1766    2157    2184    3529    1592 
   Size:        174     391      27    1345    2070

  Fragments arranged by size: 

                2070    1345     391     174      27
 
EagI C'GGCC_G

Cuts at:   1592    1592 
   Size:       4007
 
EarI CTCTTCn'nnn_

Cuts at:    905    2030    2240     905 
   Size:       1125     210    2672

  Fragments arranged by size: 

                2672    1125     210
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:   2215    2525    2671    3817    2215 
   Size:        310     146    1146    2405

  Fragments arranged by size: 

                2405    1146     310     146
 
EcoICRI GAG'CTC

Cuts at:   1019    1019 
   Size:       4007
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    814     943    1832    2264    3011    3279     814 
   Size:        129     889     432     747     268    1542

  Fragments arranged by size: 

                1542     889     747     432     268     129
 
EcoO109I rG'GnC_Cy

Cuts at:   1146    1146 
   Size:       4007
 
EcoRI G'AATT_C

Cuts at:   1011    1011 
   Size:       4007
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   2026    2026 
   Size:       4007
 
FspI TGC'GCA

Cuts at:   1788    1788 
   Size:       4007
 
HaeII r_GCGC'y

Cuts at:   1441    1689    2711    3690    1441 
   Size:        248    1022     979    1758

  Fragments arranged by size: 

                1758    1022     979     248
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1849    1879    1881    1911    1849 
   Size:         30       2      30    3945

  Fragments arranged by size: 

                3945      30      30       2
 
HincII GTy'rAC

Cuts at:    435    1046     435 
   Size:        611    3396
 
KasI G'GCGC_C

Cuts at:   1437    1685    1437 
   Size:        248    3759
 
KpnI G_GTAC'C

Cuts at:   1027    1027 
   Size:       4007
 
MluI A'CGCG_T

Cuts at:   3440    3440 
   Size:       4007
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   2678    2862    2678 
   Size:        184    3823
 
MscI TGG'CCA

Cuts at:   1768    1768 
   Size:       4007
 
MslI CAynn'nnrTG

Cuts at:   1996    2123    1996 
   Size:        127    3880
 
MspA1I CmG'CkG

Cuts at:   1118    1432    1792    2805    3050    1118 
   Size:        314     360    1013     245    2075

  Fragments arranged by size: 

                2075    1013     360     314     245
 
NaeI GCC'GGC

Cuts at:   2188    2188 
   Size:       4007
 
NarI GG'CG_CC

Cuts at:   1438    1686    1438 
   Size:        248    3759
 
NgoMIV G'CCGG_C

Cuts at:   2186    2186 
   Size:       4007
 
NsiI A_TGCA'T

Cuts at:    732     732 
   Size:       4007
 
NspI r_CATG'y

Cuts at:   1060    1560    2467    1060 
   Size:        500     907    2600

  Fragments arranged by size: 

                2600     907     500
 
PciI A'CATG_T

Cuts at:   2463    2463 
   Size:       4007
 
PfoI T'CCnGG_A

Cuts at:    896     896 
   Size:       4007
 
PmeI GTTT'AAAC

Cuts at:   1006    1006 
   Size:       4007
 
PmlI CAC'GTG

Cuts at:    386     386 
   Size:       4007
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3146    3178    3146 
   Size:         32    3975
 
PstI C_TGCA'G

Cuts at:    122    1054    1739     122 
   Size:        932     685    2390

  Fragments arranged by size: 

                2390     932     685
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       4007
 
PvuII CAG'CTG

Cuts at:   1432    1792    1432 
   Size:        360    3647
 
RsrII CG'GwC_CG

Cuts at:   2202    2202 
   Size:       4007
 
SacI G_AGCT'C

Cuts at:   1021    1021 
   Size:       4007
 
SalI G'TCGA_C

Cuts at:   1044    1044 
   Size:       4007
 
SapI GCTCTTCn'nnn_

Cuts at:   2030    2240    2030 
   Size:        210    3797
 
SbfI CC_TGCA'GG

Cuts at:   1054    1054 
   Size:       4007
 
ScaI AGT'ACT

Cuts at:   3524    3524 
   Size:       4007
 
SfcI C'TryA_G

Cuts at:     33     118    1050    1735    2728    2919      33 
   Size:         85     932     685     993     191    1121

  Fragments arranged by size: 

                1121     993     932     685     191      85
 
SfoI GGC'GCC

Cuts at:   1439    1687    1439 
   Size:        248    3759
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       4007
 
SmaI CCC'GGG

Cuts at:   1029    1029 
   Size:       4007
 
SmlI C'TyrA_G

Cuts at:    540    1162    2077    2569    2831     540 
   Size:        622     915     492     262    1716

  Fragments arranged by size: 

                1716     915     622     492     262
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       4007
 
SphI G_CATG'C

Cuts at:   1060    1560    1060 
   Size:        500    3507
 
SspI AAT'ATT

Cuts at:    276     276 
   Size:       4007
 
StyI C'CwwG_G

Cuts at:   1141    1495    1141 
   Size:        354    3653
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:   1590    1723    2182    2391    1590 
   Size:        133     459     209    3206

  Fragments arranged by size: 

                3206     459     209     133
 
TatI w'GTAC_w

Cuts at:   3522    3522 
   Size:       4007
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   3524    3556    3524 
   Size:         32    3975
 
Tth111I GACn'n_nGTC

Cuts at:   1804    1804 
   Size:       4007
 
XbaI T'CTAG_A

Cuts at:   1038    1038 
   Size:       4007
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:   1502    1502 
   Size:       4007
 
XmaI C'CCGG_G

Cuts at:   1027    1027 
   Size:       4007
 

 Enzymes that do cut:

     AccI   Acc65I     AclI     AcuI    AflII   AflIII     AgeI    AlwNI
    ApaLI     ApoI     AseI    AsiSI     AvaI    BamHI     BanI    BanII
     BbeI     BbsI     BcgI    BciVI     BclI    BfrBI     BglI    BglII
     BlpI Bme1580I     BmrI     BplI     BpmI    BpuEI     BsaI    BsaAI
    BsaBI    BsaHI    BsaWI    BseYI    BsiEI  BsiHKAI     BsmI Bsp1286I
    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BssSI   BstAPI
    BstBI    BstYI  BstZ17I    BtgZI     BtsI     DraI     DrdI     EaeI
     EagI     EarI     EciI  EcoICRI  Eco57MI EcoO109I    EcoRI     FalI
     FspI    HaeII    Hin4I   HincII     KasI     KpnI     MluI     MmeI
     MscI     MslI   MspA1I     NaeI     NarI   NgoMIV     NsiI     NspI
     PciI     PfoI     PmeI     PmlI     PpiI     PstI     PvuI    PvuII
    RsrII     SacI     SalI     SapI     SbfI     ScaI     SfcI     SfoI
     SgfI     SmaI     SmlI     SpeI     SphI     SspI     StyI    TaqII
     TatI     TstI  Tth111I     XbaI     XcmI     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AfeI     AhdI     AleI     AlfI     AloI     ApaI
     AscI    AvrII     BaeI    BbvCI    BmgBI     BmtI   Bpu10I    BsaXI
    BseRI     BsgI    BsiWI    BsmBI    BsrGI   BssHII   BstEII    BstXI
   Bsu36I     BtgI     ClaI    CspCI   DraIII    EcoNI    EcoRV     FseI
    FspAI  HindIII     HpaI     MfeI     NcoI     NdeI     NheI     NotI
     NruI     PacI     PasI    PflMI    PpuMI    PshAI     PsiI   PspOMI
    PspXI     PsrI    SacII    SanDI    SexAI     SfiI    SgrAI    SnaBI
     SrfI     StuI     SwaI     XhoI     XmnI     ZraI
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