(Linear) (Six-base) MAPSORT of: KIEE3662.seq Check: 982 from: 1 to: 2928 KIEE3662.orf With 182 enzymes: SgfI * October 24, 2019 20:50 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 1102 2928 Size: 1102 1826 AccI GT'mk_AC Cuts at: 0 587 1082 2761 2928 Size: 587 495 1679 167 Fragments arranged by size: 1679 587 495 167 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1765 1774 1915 2928 Size: 1765 9 141 1013 Fragments arranged by size: 1765 1013 141 9 AfeI AGC'GCT Cuts at: 0 502 2928 Size: 502 2426 AflIII A'CryG_T Cuts at: 0 194 2396 2928 Size: 194 2202 532 Fragments arranged by size: 2202 532 194 AhdI GACnn_n'nnGTC Cuts at: 0 960 2928 Size: 960 1968 AleI CACnn'nnGTG Cuts at: 0 688 1009 1186 2928 Size: 688 321 177 1742 Fragments arranged by size: 1742 688 321 177 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1576 2588 2928 Size: 1576 1012 340 Fragments arranged by size: 1576 1012 340 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 2113 2145 2928 Size: 2113 32 783 Fragments arranged by size: 2113 783 32 AlwNI CAG_nnn'CTG Cuts at: 0 341 621 780 1527 1578 2928 Size: 341 280 159 747 51 1350 Fragments arranged by size: 1350 747 341 280 159 51 ApaI G_GGCC'C Cuts at: 0 2097 2928 Size: 2097 831 ApaLI G'TGCA_C Cuts at: 0 1561 2206 2928 Size: 1561 645 722 Fragments arranged by size: 1561 722 645 ApoI r'AATT_y Cuts at: 0 1567 2928 Size: 1567 1361 AvaI C'yCGr_G Cuts at: 0 604 1111 1905 2302 2928 Size: 604 507 794 397 626 Fragments arranged by size: 794 626 604 507 397 BamHI G'GATC_C Cuts at: 0 2918 2928 Size: 2918 10 BanI G'GyrC_C Cuts at: 0 331 391 556 682 705 762 907 945 Size: 331 60 165 126 23 57 145 38 Cuts at: 945 1021 1920 2709 2928 Size: 76 899 789 219 Fragments arranged by size: 899 789 331 219 165 145 126 76 60 57 38 23 BanII G_rGCy'C Cuts at: 0 8 122 293 728 865 1211 1316 2097 Size: 8 114 171 435 137 346 105 781 Cuts at: 2097 2928 Size: 831 Fragments arranged by size: 831 781 435 346 171 137 114 105 8 BbeI G_GCGC'C Cuts at: 0 335 2928 Size: 335 2593 BbsI GAAGACnn'nnnn_ Cuts at: 0 1762 1903 2928 Size: 1762 141 1025 Fragments arranged by size: 1762 1025 141 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 447 481 2217 2251 2928 Size: 447 34 1736 34 677 Fragments arranged by size: 1736 677 447 34 34 BclI T'GATC_A Cuts at: 0 2066 2928 Size: 2066 862 BglI GCCn_nnn'nGGC Cuts at: 0 67 565 676 771 791 820 2036 2359 Size: 67 498 111 95 20 29 1216 323 Cuts at: 2359 2928 Size: 569 Fragments arranged by size: 1216 569 498 323 111 95 67 29 20 BlpI GC'TnA_GC Cuts at: 0 272 1354 2928 Size: 272 1082 1574 Fragments arranged by size: 1574 1082 272 Bme1580I G_kGCm'C Cuts at: 0 604 767 880 948 1565 1701 2097 2210 Size: 604 163 113 68 617 136 396 113 Cuts at: 2210 2577 2631 2714 2928 Size: 367 54 83 214 Fragments arranged by size: 617 604 396 367 214 163 136 113 113 83 68 54 BmgBI CAC'GTC Cuts at: 0 2397 2928 Size: 2397 531 BmrI ACTGGGnnnn_n' Cuts at: 0 261 2463 2928 Size: 261 2202 465 Fragments arranged by size: 2202 465 261 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 136 519 1485 2928 Size: 136 383 966 1443 Fragments arranged by size: 1443 966 383 136 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 131 1225 1351 1386 2311 2758 2928 Size: 131 1094 126 35 925 447 170 Fragments arranged by size: 1094 925 447 170 131 126 35 Bpu10I CC'TnA_GC Cuts at: 0 881 2928 Size: 881 2047 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 277 2626 2878 2928 Size: 277 2349 252 50 Fragments arranged by size: 2349 277 252 50 BsaI GGTCTCn'nnnn_ Cuts at: 0 631 2928 Size: 631 2297 BsaAI yAC'GTr Cuts at: 0 307 480 1764 2928 Size: 307 173 1284 1164 Fragments arranged by size: 1284 1164 307 173 BsaBI GATnn'nnATC Cuts at: 0 2068 2338 2923 2928 Size: 2068 270 585 5 Fragments arranged by size: 2068 585 270 5 BsaHI Gr'CG_yC Cuts at: 0 332 1045 1250 2039 2928 Size: 332 713 205 789 889 Fragments arranged by size: 889 789 713 332 205 BsaWI w'CCGG_w Cuts at: 0 2372 2928 Size: 2372 556 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 184 214 2512 2542 2928 Size: 184 30 2298 30 386 Fragments arranged by size: 2298 386 184 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 278 511 1154 2928 Size: 278 233 643 1774 Fragments arranged by size: 1774 643 278 233 BseYI C'CCAG_C Cuts at: 0 957 2686 2886 2928 Size: 957 1729 200 42 Fragments arranged by size: 1729 957 200 42 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 761 1540 2830 2928 Size: 761 779 1290 98 Fragments arranged by size: 1290 779 761 98 BsiEI CG_ry'CG Cuts at: 0 62 2783 2928 Size: 62 2721 145 Fragments arranged by size: 2721 145 62 BsiHKAI G_wGCw'C Cuts at: 0 8 122 293 887 1565 2210 2771 2928 Size: 8 114 171 594 678 645 561 157 Fragments arranged by size: 678 645 594 561 171 157 114 8 BsmI GAATG_Cn' Cuts at: 0 1129 2928 Size: 1129 1799 BsmBI CGTCTCn'nnnn_ Cuts at: 0 1257 1365 2928 Size: 1257 108 1563 Fragments arranged by size: 1563 1257 108 Bsp1286I G_dGCh'C Cuts at: 0 8 122 293 604 728 767 865 880 Size: 8 114 171 311 124 39 98 15 Cuts at: 880 887 948 1211 1316 1565 1701 2097 2210 Size: 7 61 263 105 249 136 396 113 Cuts at: 2210 2577 2631 2714 2771 2928 Size: 367 54 83 57 157 Fragments arranged by size: 396 367 311 263 249 171 157 136 124 114 113 105 98 83 61 57 54 39 15 8 7 BspHI T'CATG_A Cuts at: 0 173 2546 2928 Size: 173 2373 382 Fragments arranged by size: 2373 382 173 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 1102 2928 Size: 1102 1826 BsrBI CCG'CTC Cuts at: 0 516 2445 2696 2928 Size: 516 1929 251 232 Fragments arranged by size: 1929 516 251 232 BsrDI GCAATG_nn' Cuts at: 0 431 1251 2928 Size: 431 820 1677 Fragments arranged by size: 1677 820 431 BsrFI r'CCGG_y Cuts at: 0 646 679 1549 1923 1948 1990 2782 2928 Size: 646 33 870 374 25 42 792 146 Fragments arranged by size: 870 792 646 374 146 42 33 25 BsrGI T'GTAC_A Cuts at: 0 1880 2928 Size: 1880 1048 BssSI C'ACGA_G Cuts at: 0 2470 2691 2928 Size: 2470 221 237 Fragments arranged by size: 2470 237 221 BstAPI GCAn_nnn'nTGC Cuts at: 0 891 2928 Size: 891 2037 BstEII G'GTnAC_C Cuts at: 0 1263 2928 Size: 1263 1665 BstXI CCAn_nnnn'nTGG Cuts at: 0 1509 2517 2928 Size: 1509 1008 411 Fragments arranged by size: 1509 1008 411 BstYI r'GATC_y Cuts at: 0 1591 1820 2333 2918 2928 Size: 1591 229 513 585 10 Fragments arranged by size: 1591 585 513 229 10 BstZ17I GTA'TAC Cuts at: 0 2762 2928 Size: 2762 166 Bsu36I CC'TnA_GG Cuts at: 0 902 950 1052 2928 Size: 902 48 102 1876 Fragments arranged by size: 1876 902 102 48 BtgI C'CryG_G Cuts at: 0 56 62 506 701 1010 1232 2516 2928 Size: 56 6 444 195 309 222 1284 412 Fragments arranged by size: 1284 444 412 309 222 195 56 6 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 627 2151 2856 2928 Size: 627 1524 705 72 Fragments arranged by size: 1524 705 627 72 BtsI GCAGTG_nn' Cuts at: 0 749 781 2560 2789 2928 Size: 749 32 1779 229 139 Fragments arranged by size: 1779 749 229 139 32 DraIII CAC_nnn'GTG Cuts at: 0 2475 2928 Size: 2475 453 EaeI y'GGCC_r Cuts at: 0 53 59 668 677 1928 2189 2780 2856 Size: 53 6 609 9 1251 261 591 76 Cuts at: 2856 2928 Size: 72 Fragments arranged by size: 1251 609 591 261 76 72 53 9 6 EagI C'GGCC_G Cuts at: 0 59 2780 2928 Size: 59 2721 148 Fragments arranged by size: 2721 148 59 EarI CTCTTCn'nnn_ Cuts at: 0 716 2928 Size: 716 2212 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 978 2017 2520 2928 Size: 978 1039 503 408 Fragments arranged by size: 1039 978 503 408 EcoICRI GAG'CTC Cuts at: 0 6 120 291 2928 Size: 6 114 171 2637 Fragments arranged by size: 2637 171 114 6 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 131 1225 1351 1386 1765 1774 1915 2311 Size: 131 1094 126 35 379 9 141 396 Cuts at: 2311 2758 2928 Size: 447 170 Fragments arranged by size: 1094 447 396 379 170 141 131 126 35 9 EcoNI CCTnn'n_nnAGG Cuts at: 0 784 1513 1984 2928 Size: 784 729 471 944 Fragments arranged by size: 944 784 729 471 EcoO109I rG'GnC_Cy Cuts at: 0 156 618 954 1785 2093 2094 2928 Size: 156 462 336 831 308 1 834 Fragments arranged by size: 834 831 462 336 308 156 1 EcoRI G'AATT_C Cuts at: 0 1567 2928 Size: 1567 1361 EcoRV GAT'ATC Cuts at: 0 552 2928 Size: 552 2376 HaeII r_GCGC'y Cuts at: 0 335 411 504 571 2928 Size: 335 76 93 67 2357 Fragments arranged by size: 2357 335 93 76 67 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 946 978 1035 1067 2512 2544 2928 Size: 946 32 57 32 1445 32 384 Fragments arranged by size: 1445 946 384 57 32 32 32 HincII GTy'rAC Cuts at: 0 2241 2928 Size: 2241 687 KasI G'GCGC_C Cuts at: 0 331 2928 Size: 331 2597 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 356 1460 2489 2928 Size: 356 1104 1029 439 Fragments arranged by size: 1104 1029 439 356 MscI TGG'CCA Cuts at: 0 670 1930 2191 2928 Size: 670 1260 261 737 Fragments arranged by size: 1260 737 670 261 MslI CAynn'nnrTG Cuts at: 0 140 688 1009 1186 1778 2416 2515 2749 Size: 140 548 321 177 592 638 99 234 Cuts at: 2749 2928 Size: 179 Fragments arranged by size: 638 592 548 321 234 179 177 140 99 MspA1I CmG'CkG Cuts at: 0 58 64 109 1061 2145 2173 2345 2928 Size: 58 6 45 952 1084 28 172 583 Fragments arranged by size: 1084 952 583 172 58 45 28 6 NaeI GCC'GGC Cuts at: 0 648 681 1551 1950 1992 2784 2928 Size: 648 33 870 399 42 792 144 Fragments arranged by size: 870 792 648 399 144 42 33 NarI GG'CG_CC Cuts at: 0 332 2928 Size: 332 2596 NcoI C'CATG_G Cuts at: 0 2516 2928 Size: 2516 412 NdeI CA'TA_TG Cuts at: 0 1779 2928 Size: 1779 1149 NgoMIV G'CCGG_C Cuts at: 0 646 679 1549 1948 1990 2782 2928 Size: 646 33 870 399 42 792 146 Fragments arranged by size: 870 792 646 399 146 42 33 NotI GC'GGCC_GC Cuts at: 0 59 2928 Size: 59 2869 NspI r_CATG'y Cuts at: 0 186 198 1893 2157 2928 Size: 186 12 1695 264 771 Fragments arranged by size: 1695 771 264 186 12 PciI A'CATG_T Cuts at: 0 194 2928 Size: 194 2734 PflMI CCAn_nnn'nTGG Cuts at: 0 702 2345 2928 Size: 702 1643 583 Fragments arranged by size: 1643 702 583 PfoI T'CCnGG_A Cuts at: 0 545 1363 2609 2928 Size: 545 818 1246 319 Fragments arranged by size: 1246 818 545 319 PmlI CAC'GTG Cuts at: 0 480 2928 Size: 480 2448 PpuMI rG'GwC_Cy Cuts at: 0 156 954 1785 2928 Size: 156 798 831 1143 Fragments arranged by size: 1143 831 798 156 PspOMI G'GGCC_C Cuts at: 0 2093 2928 Size: 2093 835 PstI C_TGCA'G Cuts at: 0 108 617 2144 2928 Size: 108 509 1527 784 Fragments arranged by size: 1527 784 509 108 PvuII CAG'CTG Cuts at: 0 109 2145 2173 2928 Size: 109 2036 28 755 Fragments arranged by size: 2036 755 109 28 SacI G_AGCT'C Cuts at: 0 8 122 293 2928 Size: 8 114 171 2635 Fragments arranged by size: 2635 171 114 8 SacII CC_GC'GG Cuts at: 0 59 65 2928 Size: 59 6 2863 Fragments arranged by size: 2863 59 6 SapI GCTCTTCn'nnn_ Cuts at: 0 716 2928 Size: 716 2212 SexAI A'CCwGG_T Cuts at: 0 1508 2249 2900 2928 Size: 1508 741 651 28 Fragments arranged by size: 1508 741 651 28 SfcI C'TryA_G Cuts at: 0 104 613 1083 2140 2877 2928 Size: 104 509 470 1057 737 51 Fragments arranged by size: 1057 737 509 470 104 51 SfiI GGCCn_nnn'nGGCC Cuts at: 0 676 2928 Size: 676 2252 SfoI GGC'GCC Cuts at: 0 333 2928 Size: 333 2595 SmlI C'TyrA_G Cuts at: 0 292 2641 2893 2928 Size: 292 2349 252 35 Fragments arranged by size: 2349 292 252 35 SphI G_CATG'C Cuts at: 0 1893 2157 2928 Size: 1893 264 771 Fragments arranged by size: 1893 771 264 StuI AGG'CCT Cuts at: 0 823 1259 2928 Size: 823 436 1669 Fragments arranged by size: 1669 823 436 StyI C'CwwG_G Cuts at: 0 1025 1319 2516 2928 Size: 1025 294 1197 412 Fragments arranged by size: 1197 1025 412 294 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 243 1009 2529 2928 Size: 243 766 1520 399 Fragments arranged by size: 1520 766 399 243 TatI w'GTAC_w Cuts at: 0 197 1880 2078 2928 Size: 197 1683 198 850 Fragments arranged by size: 1683 850 198 197 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 739 771 2928 Size: 739 32 2157 Fragments arranged by size: 2157 739 32 Tth111I GACn'n_nGTC Cuts at: 0 1078 1578 2599 2928 Size: 1078 500 1021 329 Fragments arranged by size: 1078 1021 500 329 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 1326 2802 2928 Size: 1326 1476 126 Fragments arranged by size: 1476 1326 126 Enzymes that do cut: AarI AccI AcuI AfeI AflIII AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AvaI BamHI BanI BanII BbeI BbsI BcgI BclI BglI BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI DraIII EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV HaeII Hin4I HincII KasI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NotI NspI PciI PflMI PfoI PmlI PpuMI PspOMI PstI PvuII SacI SacII SapI SexAI SfcI SfiI SfoI SmlI SphI StuI StyI TaqII TatI TstI Tth111I XcmI Enzymes that do not cut: AatII Acc65I AclI AflII AgeI AscI AseI AsiSI AvrII BaeI BbvCI BciVI BfrBI BglII BmtI BsiWI BspEI BssHII BstBI ClaI CspCI DraI DrdI FalI FseI FspI FspAI HindIII HpaI KpnI MfeI MluI NheI NruI NsiI PacI PasI PmeI PpiI PshAI PsiI PspXI PsrI PvuI RsrII SalI SanDI SbfI ScaI SgfI SgrAI SmaI SnaBI SpeI SrfI SspI SwaI XbaI XhoI XmaI XmnI ZraI