Restriction map for pF1KE0216
##################################
# NO CUT ENZYMES (  159 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcsI        AflII       AgeI        AhdI        Alw44I      
AocI        Aor51HI     ApaLI       ApoI        AscI        
AseI        AsnI        Asp700I     Asp718I     AspEI       
AspI        AvaII       AviII       AvrII       BalI        
BamHI       BbrPI       BbsI        BbvCI       BcgI        
BciVI       BclI        BfaI        BfrI        BglII       
BlnI        BlpI        Bpu1102I    BpuAI       BsaAI       
BsaBI       BsaWI       BseAI       BsgI        BsiEI       
BsiWI       BsmI        Bsp1407I    BspDI       BspEI       
BspLU11I    BspMI       BsrBI       BsrDI       BsrGI       
BssHII      BssSI       Bst1107I    BstAPI      BstBI       
BstEII      BstPI       BstXI       BstYI       BstZ17I     
Bsu36I      CelII       ClaI        CpoI        DdeI        
DraI        DraIII      DrdI        DsaI        EaeI        
EagI        Eam1105I    EclXI       Eco47III    Eco52I      
Eco81I      EcoO65I     EcoRI       EcoRV       EcoT22I     
FbaI        FseI        FspI        HgaI        HincII      
HindII      HindIII     HpaI        HphI        KpnI        
KspI        MaeI        MamI        MfeI        MflI        
MluI        MluNI       MroI        MscI        MseI        
MunI        NcoI        NdeI        NheI        NotI        
NsiI        NspV        PacI        PaeR7I      PflFI       
PflMI       PinAI       PmaCI       PmeI        PmlI        
PpuMI       PshAI       PshBI       Psp1406I    PstI        
PvuI        RsrII       SacI        SacII       SalI        
SapI        SbfI        ScaI        SexAI       SfcI        
SfiI        SfuI        SgrAI       SmaI        SmlI        
SnaBI       SpeI        SphI        Sse8387I    SspBI       
SspI        SwaI        TfiI        Tru9I       Tsp509I     
Tth111I     Van91I      XbaI        XcmI        XhoI        
XhoII       XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   42 enzymes) #
###################################

AccII         397  CGCG                 CG^CG
AcyI           51  GGCGCC               G[GA]^CG[CT]C
AfaI          105  GTAC                 GT^AC
AflIII        233  ACGTGT               A^C[GA][CT]GT
AlwNI         462  CAGTCACTG            CAG[ACGT][ACGT][ACGT]^CTG
ApaI          188  GGGCCC               GGGCC^C
AvaI          403  CCCGAG               C^[CT]CG[GA]G
BanI           51  GGCGCC               G^G[CT][GA]CC
BbeI           51  GGCGCC               GGCGC^C
BcnI           98  CCGGG                CC^[GC]GG
BglI          293  GCCAGGGCGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaHI          51  GGCGCC               G[GA]^CG[CT]C
BsaI          496  GGTCTC               GGTCTC(1/5)
BseRI         412  GAGGAG               GAGGAG(10/8)
BsmBI         258  CGTCTC               CGTCTC(1/5)
BsoBI         403  CCCGAG               C^[CT]CG[GA]G
BspHI          23  TCATGA               T^CATGA
BstF5I        213  CATCC                GGATG(2/0)
BstUI         397  CGCG                 CG^CG
EarI          427  GAAGAG               CTCTTC(1/4)
EcoNI         159  CCTGTTTGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FokI          213  CATCC                GGATG(9/13)
Hin1I          51  GGCGCC               G[GA]^CG[CT]C
HinfI         357  GACTC                G^A[ACGT]TC
KasI           51  GGCGCC               G^GCGCC
Ksp632I       427  GAAGAG               CTCTTC(1/4)
MaeIII        464  GTCAC                ^GT[ACGT]AC
MslI          213  CATCCAGGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI          397  CGCG                 CG^CG
NarI           51  GGCGCC               GG^CGCC
NciI           98  CCGGG                CC^[GC]GG
NruI          396  TCGCGA               TCG^CGA
NspI           44  ACATGC               [GA]CATG^[CT]
PleI          357  GACTC                GAGTC(4/5)
PvuII         148  CAGCTG               CAG^CTG
RcaI           23  TCATGA               T^CATGA
RsaI          105  GTAC                 GT^AC
SfaNI         438  GATGC                GCATC(5/9)
SfoI           51  GGCGCC               GGC^GCC
TaqI          533  TCGA                 T^CGA
Tsp45I        464  GTCAC                ^GT[GC]AC
TthHB8I       533  TCGA                 T^CGA


###################################
# TWO CUT ENZYMES (   21 enzymes) #
###################################

AluI          149  AGCT                 AG^CT
              328  AGCT                
AlwI          400  GATCC                GGATC(4/5)
              371  GGATC               
AspHI         133  GAGCAC               G[AT]GC[AT]^C
              220  GTGCTC              
BanII         188  GGGCCC               G[GA]GC[CT]^C
              526  GAGCCC              
BbvI          150  GCTGC                GCAGC(8/12)
              555  GCTGC               
BsiHKAI       133  GAGCAC               G[AT]GC[AT]^C
              220  GTGCTC              
BsrFI          48  GCCGGC               [GA]^CCGG[CT]
               75  GCCGGC              
BsrI          561  ACTGG                ACTGG(1/-1)
              461  CCAGT               
Cfr10I         48  GCCGGC               [GA]^CCGG[CT]
               75  GCCGGC              
DraII         187  GGGGCCC              [GA]G^G[ACGT]CC[CT]
              188  GGGCCCT             
EcoO109I      187  GGGGCCC              [GA]G^G[ACGT]CC[CT]
              188  GGGCCCT             
EcoT14I        29  CCAAGG               C^C[AT][AT]GG
              501  CCTTGG              
FauI          290  CCCGC                CCCGC(4/6)
              448  CCCGC               
HaeII          51  GGCGCC               [GA]GCGC^[CT]
              473  AGCGCC              
MboII         424  GAAGA                GAAGA(8/7)
              427  GAAGA               
MspA1I        148  CAGCTG               C[AC]G^C[GT]G
              381  CAGCGG              
NaeI           48  GCCGGC               GCC^GGC
               75  GCCGGC              
NgoMIV         48  GCCGGC               G^CCGGC
               75  GCCGGC              
StuI          203  AGGCCT               AGG^CCT
              572  AGGCCT              
StyI           29  CCAAGG               C^C[AT][AT]GG
              501  CCTTGG              
TseI          150  GCTGC                G^C[AT]GC
              555  GCTGC               


#########################################
# 3 OR MORE CUT ENZYMES (   36 enzymes) #
#########################################

AciI        BmyI        BpmI        BsaJI       BsiYI       
BslI        BsmAI       BsmFI       Bsp1286I    BssKI       
BstNI       Cac8I       CfoI        Cfr13I      DpnII       
EcoRII      Fnu4HI      HaeIII      HapII       HhaI        
HinP1I      HpaII       ItaI        MaeII       MboI        
MnlI        MspI        MvaI        MwoI        NdeII       
NlaIII      NlaIV       Sau3AI      Sau96I      ScrFI       
TspRI       
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