Restriction map for pF1KE0216
 (Linear) (Six-base) MAPSORT of: KIEE0216.seq  Check: 2522  from: 1  to: 582



KIEE0216.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:11  ..

AflIII A'CryG_T

Cuts at:      0     233     582 
   Size:        233     349
 
AlwNI CAG_nnn'CTG

Cuts at:      0     467     582 
   Size:        467     115
 
ApaI G_GGCC'C

Cuts at:      0     192     582 
   Size:        192     390
 
AvaI C'yCGr_G

Cuts at:      0     403     582 
   Size:        403     179
 
BanI G'GyrC_C

Cuts at:      0      51     582 
   Size:         51     531
 
BanII G_rGCy'C

Cuts at:      0     192     530     582 
   Size:        192     338      52

  Fragments arranged by size: 

                 338     192      52
 
BbeI G_GCGC'C

Cuts at:      0      55     582 
   Size:         55     527
 
BglI GCCn_nnn'nGGC

Cuts at:      0     299     582 
   Size:        299     283
 
Bme1580I G_kGCm'C

Cuts at:      0     192     582 
   Size:        192     390
 
BmgBI CAC'GTC

Cuts at:      0      90     171     234     582 
   Size:         90      81      63     348

  Fragments arranged by size: 

                 348      90      81      63
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     222     582 
   Size:        222     360
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      37      79     444     582 
   Size:         37      42     365     138

  Fragments arranged by size: 

                 365     138      42      37
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     502     582 
   Size:        502      80
 
BsaHI Gr'CG_yC

Cuts at:      0      52     582 
   Size:         52     530
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0      79     109     582 
   Size:         79      30     473

  Fragments arranged by size: 

                 473      79      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     427     582 
   Size:        427     155
 
BseYI C'CCAG_C

Cuts at:      0     146     390     442     582 
   Size:        146     244      52     140

  Fragments arranged by size: 

                 244     146     140      52
 
BsiHKAI G_wGCw'C

Cuts at:      0     137     224     582 
   Size:        137      87     358

  Fragments arranged by size: 

                 358     137      87
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     264     582 
   Size:        264     318
 
Bsp1286I G_dGCh'C

Cuts at:      0     137     192     224     530     582 
   Size:        137      55      32     306      52

  Fragments arranged by size: 

                 306     137      55      52      32
 
BspHI T'CATG_A

Cuts at:      0      23     582 
   Size:         23     559
 
BsrFI r'CCGG_y

Cuts at:      0      48      75     582 
   Size:         48      27     507

  Fragments arranged by size: 

                 507      48      27
 
BtsI GCAGTG_nn'

Cuts at:      0      86     281     582 
   Size:         86     195     301

  Fragments arranged by size: 

                 301     195      86
 
EarI CTCTTCn'nnn_

Cuts at:      0     422     582 
   Size:        422     160
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      37      79     444     582 
   Size:         37      42     365     138

  Fragments arranged by size: 

                 365     138      42      37
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     163     582 
   Size:        163     419
 
EcoO109I rG'GnC_Cy

Cuts at:      0     188     189     582 
   Size:        188       1     393

  Fragments arranged by size: 

                 393     188       1
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     345     582 
   Size:        345     237
 
HaeII r_GCGC'y

Cuts at:      0      55     477     582 
   Size:         55     422     105

  Fragments arranged by size: 

                 422     105      55
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      79     111     242     274     582 
   Size:         79      32     131      32     308

  Fragments arranged by size: 

                 308     131      79      32      32
 
KasI G'GCGC_C

Cuts at:      0      51     582 
   Size:         51     531
 
MslI CAynn'nnrTG

Cuts at:      0     217     582 
   Size:        217     365
 
MspA1I CmG'CkG

Cuts at:      0     150     383     582 
   Size:        150     233     199

  Fragments arranged by size: 

                 233     199     150
 
NaeI GCC'GGC

Cuts at:      0      50      77     582 
   Size:         50      27     505

  Fragments arranged by size: 

                 505      50      27
 
NarI GG'CG_CC

Cuts at:      0      52     582 
   Size:         52     530
 
NgoMIV G'CCGG_C

Cuts at:      0      48      75     582 
   Size:         48      27     507

  Fragments arranged by size: 

                 507      48      27
 
NruI TCG'CGA

Cuts at:      0     398     582 
   Size:        398     184
 
NspI r_CATG'y

Cuts at:      0      48     582 
   Size:         48     534
 
PspOMI G'GGCC_C

Cuts at:      0     188     582 
   Size:        188     394
 
PvuII CAG'CTG

Cuts at:      0     150     582 
   Size:        150     432
 
SfoI GGC'GCC

Cuts at:      0      53     582 
   Size:         53     529
 
StuI AGG'CCT

Cuts at:      0     205     574     582 
   Size:        205     369       8

  Fragments arranged by size: 

                 369     205       8
 
StyI C'CwwG_G

Cuts at:      0      29     501     582 
   Size:         29     472      81

  Fragments arranged by size: 

                 472      81      29
 
TatI w'GTAC_w

Cuts at:      0     104     582 
   Size:        104     478
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     220     252     582 
   Size:        220      32     330

  Fragments arranged by size: 

                 330     220      32
 

 Enzymes that do cut:

   AflIII    AlwNI     ApaI     AvaI     BanI    BanII     BbeI     BglI
 Bme1580I    BmgBI     BplI     BpmI     BsaI    BsaHI    BsaXI    BseRI
    BseYI  BsiHKAI    BsmBI Bsp1286I    BspHI    BsrFI     BtsI     EarI
  Eco57MI    EcoNI EcoO109I     FalI    HaeII    Hin4I     KasI     MslI
   MspA1I     NaeI     NarI   NgoMIV     NruI     NspI   PspOMI    PvuII
     SfoI     StuI     StyI     TatI     TstI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
     AgeI     AhdI     AleI     AlfI     AloI    ApaLI     ApoI     AscI
     AseI    AsiSI    AvrII     BaeI    BamHI     BbsI    BbvCI     BcgI
    BciVI     BclI    BfrBI    BglII     BlpI     BmrI     BmtI   Bpu10I
    BpuEI    BsaAI    BsaBI    BsaWI     BsgI    BsiEI    BsiWI     BsmI
    BspEI    BspMI    BsrBI    BsrDI    BsrGI   BssHII    BssSI   BstAPI
    BstBI   BstEII    BstXI    BstYI  BstZ17I   Bsu36I     BtgI    BtgZI
     ClaI    CspCI     DraI   DraIII     DrdI     EaeI     EagI     EciI
  EcoICRI    EcoRI    EcoRV     FseI     FspI    FspAI   HincII  HindIII
     HpaI     KpnI     MfeI     MluI     MmeI     MscI     NcoI     NdeI
     NheI     NotI     NsiI     PacI     PasI     PciI    PflMI     PfoI
     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI    PspXI     PsrI
     PstI     PvuI    RsrII     SacI    SacII     SalI    SanDI     SapI
     SbfI     ScaI    SexAI     SfcI     SfiI     SgfI    SgrAI     SmaI
     SmlI    SnaBI     SpeI     SphI     SrfI     SspI     SwaI    TaqII
  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI
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