################################## # NO CUT ENZYMES ( 130 enzymes) # ################################## Acc65I AccI AfaI AflIII AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AviII AvrII BalI BanI BbeI BbrPI BbvCI BcgI BglII BlnI BlpI BpmI Bpu1102I BsaAI BsaI BsgI BsiEI BsiHKAI BsiWI Bsp1407I BspDI BspHI BspLU11I BspMI BsrGI BssSI Bst1107I BstBI BstEII BstPI BstZ17I Bsu36I CelII ClaI DdeI DraI DraIII DrdI EaeI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT22I FseI FspI HaeII HincII HindII HpaI KasI KpnI Ksp632I KspI MfeI MluI MluNI MscI MslI MunI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PflFI PinAI PmaCI PmeI PmlI PpuMI PshBI PstI PvuI PvuII RcaI RsaI SacI SacII SalI SapI SbfI ScaI SfiI SfoI SfuI SgrAI SnaBI SpeI SphI Sse8387I SspBI StuI SwaI Tth111I XbaI XcmI XmnI ################################### # ONE CUT ENZYMES ( 56 enzymes) # ################################### AatII 961 GACGTC GACGT^C AccIII 989 TCCGGA T^CCGGA AclI 692 AACGTT AA^CGTT AflII 954 CTTAAG C^TTAAG ApaI 383 GGGCCC GGGCC^C BamHI 942 GGATCC G^GATCC BanII 383 GGGCCC G[GA]GC[CT]^C BbsI 599 GAAGAC GAAGAC(2/6) BciVI 1089 GGATAC GTATCC(6/5) BclI 275 TGATCA T^GATCA BfaI 928 CTAG C^TAG BfrI 954 CTTAAG C^TTAAG BglI 385 GCCCCCATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BmyI 383 GGGCCC G[AGT]GC[ACT]^C BpuAI 599 GAAGAC GAAGAC(2/6) BsaBI 201 GATGCGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaWI 989 TCCGGA [AT]^CCGG[AT] BseAI 989 TCCGGA T^CCGGA BsmBI 959 GAGACG CGTCTC(1/5) BsmFI 1004 GGGAC GGGAC(10/14) BsmI 477 GAATGC GAATGC(1/-1) Bsp1286I 383 GGGCCC G[AGT]GC[ACT]^C BspEI 989 TCCGGA T^CCGGA BsrBI 174 GAGCGG CCGCTC(-3/-3) BsrDI 845 GCAATG GCAATG(2/0) BsrFI 488 ACCGGC [GA]^CCGG[CT] BsrI 507 ACTGG ACTGG(1/-1) BstAPI 1116 GCAGCTTATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstXI 345 CCAACAACATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Cfr10I 488 ACCGGC [GA]^CCGG[CT] CpoI 22 CGGTCCG CG^G[AT]CCG DraII 383 GGGCCCC [GA]G^G[ACGT]CC[CT] EcoO109I 383 GGGCCCC [GA]G^G[ACGT]CC[CT] FauI 166 GCGGG CCCGC(4/6) FbaI 275 TGATCA T^GATCA HgaI 492 GCGTC GACGC(5/10) HindIII 313 AAGCTT A^AGCTT MaeI 928 CTAG C^TAG MamI 201 GATGCGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MroI 989 TCCGGA T^CCGGA MspA1I 465 CAGCGG C[AC]G^C[GT]G NcoI 389 CCATGG C^CATGG PaeR7I 305 CTCGAG C^TCGAG PflMI 399 CCAAGGAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PleI 799 GAGTC GAGTC(4/5) PshAI 487 GACCGGCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC Psp1406I 692 AACGTT AA^CGTT RsrII 22 CGGTCCG CG^G[AT]CCG SexAI 758 ACCAGGT A^CC[AT]GGT SmaI 380 CCCGGG CCC^GGG SspI 524 AATATT AAT^ATT TfiI 514 GAATC G^A[AT]TC Van91I 399 CCAAGGAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XhoI 305 CTCGAG C^TCGAG XmaCI 380 CCCGGG C^CCGGG XmaI 380 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 17 enzymes) # ################################### AcyI 491 GGCGTC G[GA]^CG[CT]C 961 GACGTC AluI 314 AGCT AG^CT 1118 AGCT AvaII 23 GGTCC G^G[AT]CC 1005 GGACC BsaHI 491 GGCGTC G[GA]^CG[CT]C 961 GACGTC BseRI 244 GAGGAG GAGGAG(10/8) 889 GAGGAG BstYI 149 AGATCC [GA]^GATC[CT] 942 GGATCC DsaI 213 CCACGG C^C[GA][CT]GG 389 CCATGG HaeIII 72 GGCC GG^CC 384 GGCC Hin1I 491 GGCGTC G[GA]^CG[CT]C 961 GACGTC HinfI 514 GAATC G^A[ACGT]TC 799 GAGTC MaeIII 718 GTGAC ^GT[ACGT]AC 1079 GTGAC MflI 149 AGATCC [GA]^GATC[CT] 942 GGATCC MseI 868 TTAA T^TAA 955 TTAA SfcI 264 CTACAG C^T[GA][CT]AG 1126 CTACAG Tru9I 868 TTAA T^TAA 955 TTAA Tsp45I 718 GTGAC ^GT[GC]AC 1079 GTGAC XhoII 149 AGATCC [GA]^GATC[CT] 942 GGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 55 enzymes) # ######################################### AccII AciI AcsI AlwI ApoI AvaI BbvI BcnI BsaJI BsiYI BslI BsmAI BsoBI BssHII BssKI BstF5I BstNI BstUI Cac8I CfoI Cfr13I DpnII EcoRII EcoT14I Fnu4HI FokI HapII HhaI HinP1I HpaII HphI ItaI MaeII MboI MboII MnlI MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV Sau3AI Sau96I ScrFI SfaNI SmlI StyI TaqI TseI Tsp509I TspRI TthHB8I