(Circular) (Six-base) MAPSORT of: pF1KE6234.seq Check: 6294 from: 1 to: 3998 pF1KE6234 3998 bp With 182 enzymes: SgfI * July 20, 2018 17:51 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 493 493 Size: 3998 AccI GT'mk_AC Cuts at: 1036 1036 Size: 3998 Acc65I G'GTAC_C Cuts at: 311 1014 311 Size: 703 3295 AclI AA'CG_TT Cuts at: 3838 3838 Size: 3998 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1823 2255 3002 3270 1823 Size: 432 747 268 2551 Fragments arranged by size: 2551 747 432 268 AflIII A'CryG_T Cuts at: 1350 2454 3431 1350 Size: 1104 977 1917 Fragments arranged by size: 1917 1104 977 AgeI A'CCGG_T Cuts at: 2386 2386 Size: 3998 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 560 592 560 Size: 32 3966 AlwNI CAG_nnn'CTG Cuts at: 1303 2870 1303 Size: 1567 2431 ApaLI G'TGCA_C Cuts at: 2768 2768 Size: 3998 ApoI r'AATT_y Cuts at: 1002 3351 3607 1002 Size: 2349 256 1393 Fragments arranged by size: 2349 1393 256 AseI AT'TA_AT Cuts at: 20 20 Size: 3998 AsiSI GCG_AT'CGC Cuts at: 104 104 Size: 3998 AvaI C'yCGr_G Cuts at: 1018 1018 Size: 3998 BamHI G'GATC_C Cuts at: 446 1023 446 Size: 577 3421 BanI G'GyrC_C Cuts at: 311 378 717 1014 1428 1676 1711 311 Size: 67 339 297 414 248 35 2598 Fragments arranged by size: 2598 414 339 297 248 67 35 BanII G_rGCy'C Cuts at: 237 565 1012 2042 237 Size: 328 447 1030 2193 Fragments arranged by size: 2193 1030 447 328 BbeI G_GCGC'C Cuts at: 1432 1680 1432 Size: 248 3750 BbsI GAAGACnn'nnnn_ Cuts at: 173 621 813 982 173 Size: 448 192 169 3189 Fragments arranged by size: 3189 448 192 169 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 101 135 101 Size: 34 3964 BciVI GTATCCnnnnn_n' Cuts at: 1896 2657 3998 1896 Size: 761 1341 1896 Fragments arranged by size: 1896 1341 761 BclI T'GATC_A Cuts at: 779 1518 779 Size: 739 3259 BglII A'GATC_T Cuts at: 1513 3550 1513 Size: 2037 1961 BlpI GC'TnA_GC Cuts at: 1110 1110 Size: 3998 Bme1580I G_kGCm'C Cuts at: 722 1623 1716 2772 722 Size: 901 93 1056 1948 Fragments arranged by size: 1948 1056 901 93 BmrI ACTGGGnnnn_n' Cuts at: 564 1210 1291 1626 3770 564 Size: 646 81 335 2144 792 Fragments arranged by size: 2144 792 646 335 81 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 146 2365 146 Size: 2219 1779 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 587 976 587 Size: 389 3609 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 1174 2053 2545 2843 1174 Size: 879 492 298 2329 Fragments arranged by size: 2329 879 492 298 BsaI GGTCTCn'nnnn_ Cuts at: 117 365 3702 117 Size: 248 3337 413 Fragments arranged by size: 3337 413 248 BsaAI yAC'GTr Cuts at: 838 1351 838 Size: 513 3485 BsaBI GATnn'nnATC Cuts at: 6 1512 3549 6 Size: 1506 2037 455 Fragments arranged by size: 2037 1506 455 BsaHI Gr'CG_yC Cuts at: 1429 1677 2379 3875 1429 Size: 248 702 1496 1552 Fragments arranged by size: 1552 1496 702 248 BsaWI w'CCGG_w Cuts at: 449 689 1187 1708 2386 2660 2807 3218 449 Size: 240 498 521 678 274 147 411 1229 Fragments arranged by size: 1229 678 521 498 411 274 240 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 292 322 560 590 292 Size: 30 238 30 3700 Fragments arranged by size: 3700 238 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 148 151 154 157 160 529 571 592 964 Size: 3 3 3 3 369 42 21 372 Cuts at: 964 971 148 Size: 7 3175 Fragments arranged by size: 3175 372 369 42 21 7 3 3 3 3 BseYI C'CCAG_C Cuts at: 136 1423 2181 2758 3254 136 Size: 1287 758 577 496 880 Fragments arranged by size: 1287 880 758 577 496 BsiEI CG_ry'CG Cuts at: 104 1540 1586 2794 104 Size: 1436 46 1208 1308 Fragments arranged by size: 1436 1308 1208 46 BsiHKAI G_wGCw'C Cuts at: 602 1012 1790 1980 2772 602 Size: 410 778 190 792 1828 Fragments arranged by size: 1828 792 778 410 190 Bsp1286I G_dGCh'C Cuts at: 237 565 602 722 1012 1623 1716 1790 1980 Size: 328 37 120 290 611 93 74 190 Cuts at: 1980 2042 2772 237 Size: 62 730 1463 Fragments arranged by size: 1463 730 611 328 290 190 120 93 74 62 37 BspEI T'CCGG_A Cuts at: 449 3218 449 Size: 2769 1229 BspHI T'CATG_A Cuts at: 3174 3174 Size: 3998 BspMI ACCTGCnnnn'nnnn_ Cuts at: 493 1048 1262 1564 1945 493 Size: 555 214 302 381 2546 Fragments arranged by size: 2546 555 381 302 214 BsrBI CCG'CTC Cuts at: 50 2290 2344 3828 3993 50 Size: 2240 54 1484 165 55 Fragments arranged by size: 2240 1484 165 55 54 BsrDI GCAATG_nn' Cuts at: 530 1910 530 Size: 1380 2618 BsrFI r'CCGG_y Cuts at: 753 1996 2177 2386 753 Size: 1243 181 209 2365 Fragments arranged by size: 2365 1243 209 181 BssSI C'ACGA_G Cuts at: 2269 2627 2269 Size: 358 3640 BstAPI GCAn_nnn'nTGC Cuts at: 1375 1375 Size: 3998 BstBI TT'CG_AA Cuts at: 2359 2359 Size: 3998 BstYI r'GATC_y Cuts at: 1 446 543 1023 1490 1513 1848 2094 3106 Size: 445 97 480 467 23 335 246 1012 Cuts at: 3106 3192 3204 3550 1 Size: 86 12 346 449 Fragments arranged by size: 1012 480 467 449 445 346 335 246 97 86 23 12 BtgI C'CryG_G Cuts at: 107 107 Size: 3998 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 1948 2129 2292 1948 Size: 181 163 3654 Fragments arranged by size: 3654 181 163 BtsI GCAGTG_nn' Cuts at: 1241 1241 Size: 3998 DraI TTT'AAA Cuts at: 997 997 Size: 3998 DrdI GACnn_nn'nnGTC Cuts at: 1704 2562 1704 Size: 858 3140 EaeI y'GGCC_r Cuts at: 1583 1757 2148 2175 3520 1583 Size: 174 391 27 1345 2061 Fragments arranged by size: 2061 1345 391 174 27 EagI C'GGCC_G Cuts at: 1583 1583 Size: 3998 EarI CTCTTCn'nnn_ Cuts at: 824 852 903 2021 2231 824 Size: 28 51 1118 210 2591 Fragments arranged by size: 2591 1118 210 51 28 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 2206 2516 2662 3808 2206 Size: 310 146 1146 2396 Fragments arranged by size: 2396 1146 310 146 EcoICRI GAG'CTC Cuts at: 1010 1010 Size: 3998 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 587 976 1823 2255 3002 3270 587 Size: 389 847 432 747 268 1315 Fragments arranged by size: 1315 847 747 432 389 268 EcoNI CCTnn'n_nnAGG Cuts at: 327 327 Size: 3998 EcoO109I rG'GnC_Cy Cuts at: 1137 1137 Size: 3998 EcoRI G'AATT_C Cuts at: 1002 1002 Size: 3998 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2017 2017 Size: 3998 FspI TGC'GCA Cuts at: 1779 1779 Size: 3998 HaeII r_GCGC'y Cuts at: 1432 1680 2702 3681 1432 Size: 248 1022 979 1749 Fragments arranged by size: 1749 1022 979 248 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 212 244 666 684 698 716 1840 1870 1872 Size: 32 422 18 14 18 1124 30 2 Cuts at: 1872 1902 212 Size: 30 2308 Fragments arranged by size: 2308 1124 422 32 30 30 18 18 14 2 HincII GTy'rAC Cuts at: 1037 1037 Size: 3998 HindIII A'AGCT_T Cuts at: 114 114 Size: 3998 KasI G'GCGC_C Cuts at: 1428 1676 1428 Size: 248 3750 KpnI G_GTAC'C Cuts at: 315 1018 315 Size: 703 3295 MluI A'CGCG_T Cuts at: 3431 3431 Size: 3998 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 2669 2853 2669 Size: 184 3814 MscI TGG'CCA Cuts at: 1759 1759 Size: 3998 MslI CAynn'nnrTG Cuts at: 301 1987 2114 301 Size: 1686 127 2185 Fragments arranged by size: 2185 1686 127 MspA1I CmG'CkG Cuts at: 140 1109 1423 1783 2796 3041 140 Size: 969 314 360 1013 245 1097 Fragments arranged by size: 1097 1013 969 360 314 245 NaeI GCC'GGC Cuts at: 2179 2179 Size: 3998 NarI GG'CG_CC Cuts at: 1429 1677 1429 Size: 248 3750 NcoI C'CATG_G Cuts at: 107 107 Size: 3998 NgoMIV G'CCGG_C Cuts at: 2177 2177 Size: 3998 NspI r_CATG'y Cuts at: 1051 1551 2458 1051 Size: 500 907 2591 Fragments arranged by size: 2591 907 500 PciI A'CATG_T Cuts at: 2454 2454 Size: 3998 PmeI GTTT'AAAC Cuts at: 997 997 Size: 3998 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 560 592 3137 3169 560 Size: 32 2545 32 1389 Fragments arranged by size: 2545 1389 32 32 PsiI TTA'TAA Cuts at: 860 860 Size: 3998 PstI C_TGCA'G Cuts at: 611 644 1045 1730 611 Size: 33 401 685 2879 Fragments arranged by size: 2879 685 401 33 PvuI CG_AT'CG Cuts at: 104 104 Size: 3998 PvuII CAG'CTG Cuts at: 140 1423 1783 140 Size: 1283 360 2355 Fragments arranged by size: 2355 1283 360 RsrII CG'GwC_CG Cuts at: 2193 2193 Size: 3998 SacI G_AGCT'C Cuts at: 1012 1012 Size: 3998 SalI G'TCGA_C Cuts at: 1035 1035 Size: 3998 SapI GCTCTTCn'nnn_ Cuts at: 824 2021 2231 824 Size: 1197 210 2591 Fragments arranged by size: 2591 1197 210 SbfI CC_TGCA'GG Cuts at: 1045 1045 Size: 3998 ScaI AGT'ACT Cuts at: 3515 3515 Size: 3998 SfcI C'TryA_G Cuts at: 33 290 345 607 640 1041 1726 2719 2910 Size: 257 55 262 33 401 685 993 191 Cuts at: 2910 33 Size: 1121 Fragments arranged by size: 1121 993 685 401 262 257 191 55 33 SfoI GGC'GCC Cuts at: 1430 1678 1430 Size: 248 3750 SgfI GCG_AT'CGC Cuts at: 104 104 Size: 3998 SmaI CCC'GGG Cuts at: 1020 1020 Size: 3998 SmlI C'TyrA_G Cuts at: 1153 2068 2560 2822 1153 Size: 915 492 262 2329 Fragments arranged by size: 2329 915 492 262 SnaBI TAC'GTA Cuts at: 838 838 Size: 3998 SpeI A'CTAG_T Cuts at: 67 67 Size: 3998 SphI G_CATG'C Cuts at: 1051 1551 1051 Size: 500 3498 StyI C'CwwG_G Cuts at: 107 767 1132 1486 107 Size: 660 365 354 2619 Fragments arranged by size: 2619 660 365 354 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 546 955 1581 1714 2173 2382 546 Size: 409 626 133 459 209 2162 Fragments arranged by size: 2162 626 459 409 209 133 TatI w'GTAC_w Cuts at: 644 3513 644 Size: 2869 1129 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 292 324 3515 3547 292 Size: 32 3191 32 743 Fragments arranged by size: 3191 743 32 32 Tth111I GACn'n_nGTC Cuts at: 1795 1795 Size: 3998 XbaI T'CTAG_A Cuts at: 1029 1029 Size: 3998 XcmI CCAnnnn_n'nnnnTGG Cuts at: 1493 1493 Size: 3998 XmaI C'CCGG_G Cuts at: 1018 1018 Size: 3998 Enzymes that do cut: AarI AccI Acc65I AclI AcuI AflIII AgeI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglII BlpI Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII KasI KpnI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI PciI PmeI PpiI PsiI PstI PvuI PvuII RsrII SacI SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI SnaBI SpeI SphI StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI Enzymes that do not cut: AatII AfeI AflII AhdI AleI AlfI ApaI AscI AvrII BaeI BbvCI BfrBI BglI BmgBI BmtI Bpu10I BsgI BsiWI BsmI BsmBI BsrGI BssHII BstEII BstXI BstZ17I Bsu36I ClaI CspCI DraIII EcoRV FseI FspAI HpaI MfeI NdeI NheI NotI NruI NsiI PacI PasI PflMI PfoI PmlI PpuMI PshAI PspOMI PspXI PsrI SacII SanDI SexAI SfiI SgrAI SrfI SspI StuI SwaI XhoI XmnI ZraI