Restriction map for
pF1KE2155
(Circular) (Six-base) MAPSORT of: pF1KE2155.seq Check: 4786 from: 1 to: 4247
pF1KE2155 4247 bp
With 182 enzymes: SgfI *
August 25, 2016 10:58 ..
AatII G_ACGT'C
Cuts at: 1037 1037
Size: 4247
AccI GT'mk_AC
Cuts at: 1285 1285
Size: 4247
Acc65I G'GTAC_C
Cuts at: 1263 1263
Size: 4247
AclI AA'CG_TT
Cuts at: 4087 4087
Size: 4247
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 513 2072 2504 3251 3519 513
Size: 1559 432 747 268 1241
Fragments arranged by size:
1559 1241 747 432 268
AflIII A'CryG_T
Cuts at: 1599 2703 3680 1599
Size: 1104 977 2166
Fragments arranged by size:
2166 1104 977
AgeI A'CCGG_T
Cuts at: 2635 2635
Size: 4247
AleI CACnn'nnGTG
Cuts at: 220 220
Size: 4247
AlwNI CAG_nnn'CTG
Cuts at: 1552 3119 1552
Size: 1567 2680
ApaLI G'TGCA_C
Cuts at: 3017 3017
Size: 4247
ApoI r'AATT_y
Cuts at: 1251 3600 3856 1251
Size: 2349 256 1642
Fragments arranged by size:
2349 1642 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4247
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4247
AvaI C'yCGr_G
Cuts at: 1267 1267
Size: 4247
BamHI G'GATC_C
Cuts at: 1272 1272
Size: 4247
BanI G'GyrC_C
Cuts at: 1263 1677 1925 1960 1263
Size: 414 248 35 3550
Fragments arranged by size:
3550 414 248 35
BanII G_rGCy'C
Cuts at: 689 1007 1261 2291 689
Size: 318 254 1030 2645
Fragments arranged by size:
2645 1030 318 254
BbeI G_GCGC'C
Cuts at: 1681 1929 1681
Size: 248 3999
BbsI GAAGACnn'nnnn_
Cuts at: 468 912 921 1166 468
Size: 444 9 245 3549
Fragments arranged by size:
3549 444 245 9
BbvCI CC'TCA_GC
Cuts at: 1147 1147
Size: 4247
BciVI GTATCCnnnnn_n'
Cuts at: 650 2145 2906 4247 650
Size: 1495 761 1341 650
Fragments arranged by size:
1495 1341 761 650
BclI T'GATC_A
Cuts at: 899 1767 899
Size: 868 3379
BglI GCCn_nnn'nGGC
Cuts at: 329 329
Size: 4247
BglII A'GATC_T
Cuts at: 1762 3799 1762
Size: 2037 2210
BlpI GC'TnA_GC
Cuts at: 1359 1359
Size: 4247
Bme1580I G_kGCm'C
Cuts at: 615 1872 1965 3021 615
Size: 1257 93 1056 1841
Fragments arranged by size:
1841 1257 1056 93
BmgBI CAC'GTC
Cuts at: 264 264
Size: 4247
BmrI ACTGGGnnnn_n'
Cuts at: 337 1234 1459 1540 1875 4019 337
Size: 897 225 81 335 2144 565
Fragments arranged by size:
2144 897 565 335 225 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2614 2614
Size: 4247
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 688 844 688
Size: 156 4091
Bpu10I CC'TnA_GC
Cuts at: 1147 1147
Size: 4247
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 358 1423 2302 2794 3092 358
Size: 1065 879 492 298 1513
Fragments arranged by size:
1513 1065 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 1213 3951 1213
Size: 2738 1509
BsaAI yAC'GTr
Cuts at: 573 1600 573
Size: 1027 3220
BsaBI GATnn'nnATC
Cuts at: 6 793 1761 3798 6
Size: 787 968 2037 455
Fragments arranged by size:
2037 968 787 455
BsaHI Gr'CG_yC
Cuts at: 1034 1097 1678 1926 2628 4124 1034
Size: 63 581 248 702 1496 1157
Fragments arranged by size:
1496 1157 702 581 248 63
BsaWI w'CCGG_w
Cuts at: 1436 1957 2635 2909 3056 3467 1436
Size: 521 678 274 147 411 2216
Fragments arranged by size:
2216 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 702 732 702
Size: 30 4217
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 723 1188 723
Size: 465 3782
BseYI C'CCAG_C
Cuts at: 1672 2430 3007 3503 1672
Size: 758 577 496 2416
Fragments arranged by size:
2416 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1789 1835 3043 104
Size: 1685 46 1208 1308
Fragments arranged by size:
1685 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 253 674 689 1007 1261 2039 2229 3021 253
Size: 421 15 318 254 778 190 792 1479
Fragments arranged by size:
1479 792 778 421 318 254 190 15
Bsp1286I G_dGCh'C
Cuts at: 253 615 674 689 1007 1261 1872 1965 2039
Size: 362 59 15 318 254 611 93 74
Cuts at: 2039 2229 2291 3021 253
Size: 190 62 730 1479
Fragments arranged by size:
1479 730 611 362 318 254 190 93
74 62 59 15
BspEI T'CCGG_A
Cuts at: 3467 3467
Size: 4247
BspHI T'CATG_A
Cuts at: 3423 3423
Size: 4247
BspMI ACCTGCnnnn'nnnn_
Cuts at: 404 1297 1511 1813 2194 404
Size: 893 214 302 381 2457
Fragments arranged by size:
2457 893 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2539 2593 4077 4242 50
Size: 2489 54 1484 165 55
Fragments arranged by size:
2489 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 339 2159 339
Size: 1820 2427
BsrFI r'CCGG_y
Cuts at: 2245 2426 2635 2245
Size: 181 209 3857
Fragments arranged by size:
3857 209 181
BsrGI T'GTAC_A
Cuts at: 692 692
Size: 4247
BssSI C'ACGA_G
Cuts at: 2518 2876 2518
Size: 358 3889
BstAPI GCAn_nnn'nTGC
Cuts at: 729 1624 729
Size: 895 3352
BstBI TT'CG_AA
Cuts at: 2608 2608
Size: 4247
BstXI CCAn_nnnn'nTGG
Cuts at: 114 780 1209 114
Size: 666 429 3152
Fragments arranged by size:
3152 666 429
BstYI r'GATC_y
Cuts at: 1 1272 1739 1762 2097 2343 3355 3441 3453
Size: 1271 467 23 335 246 1012 86 12
Cuts at: 3453 3799 1
Size: 346 449
Fragments arranged by size:
1271 1012 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 4247
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 742 747 888 1008 2197 2378 2541 742
Size: 5 141 120 1189 181 163 2448
Fragments arranged by size:
2448 1189 181 163 141 120 5
BtsI GCAGTG_nn'
Cuts at: 723 1174 1490 723
Size: 451 316 3480
Fragments arranged by size:
3480 451 316
DraI TTT'AAA
Cuts at: 276 1246 276
Size: 970 3277
DrdI GACnn_nn'nnGTC
Cuts at: 1953 2811 1953
Size: 858 3389
EaeI y'GGCC_r
Cuts at: 461 575 818 971 1832 2006 2397 2424 3769
Size: 114 243 153 861 174 391 27 1345
Cuts at: 3769 461
Size: 939
Fragments arranged by size:
1345 939 861 391 243 174 153 114
27
EagI C'GGCC_G
Cuts at: 1832 1832
Size: 4247
EarI CTCTTCn'nnn_
Cuts at: 430 1123 1132 2270 2480 430
Size: 693 9 1138 210 2197
Fragments arranged by size:
2197 1138 693 210 9
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2455 2765 2911 4057 2455
Size: 310 146 1146 2645
Fragments arranged by size:
2645 1146 310 146
EcoICRI GAG'CTC
Cuts at: 687 1005 1259 687
Size: 318 254 3675
Fragments arranged by size:
3675 318 254
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 513 688 844 2072 2504 3251 3519 513
Size: 175 156 1228 432 747 268 1241
Fragments arranged by size:
1241 1228 747 432 268 175 156
EcoNI CCTnn'n_nnAGG
Cuts at: 859 1150 859
Size: 291 3956
EcoO109I rG'GnC_Cy
Cuts at: 1134 1386 1134
Size: 252 3995
EcoRI G'AATT_C
Cuts at: 1251 1251
Size: 4247
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 519 540 2266 519
Size: 21 1726 2500
Fragments arranged by size:
2500 1726 21
FspI TGC'GCA
Cuts at: 2028 2028
Size: 4247
HaeII r_GCGC'y
Cuts at: 1197 1681 1929 2951 3930 1197
Size: 484 248 1022 979 1514
Fragments arranged by size:
1514 1022 979 484 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 725 757 2089 2119 2121 2151 725
Size: 32 1332 30 2 30 2821
Fragments arranged by size:
2821 1332 32 30 30 2
HincII GTy'rAC
Cuts at: 350 1071 1286 350
Size: 721 215 3311
Fragments arranged by size:
3311 721 215
KasI G'GCGC_C
Cuts at: 1677 1925 1677
Size: 248 3999
KpnI G_GTAC'C
Cuts at: 1267 1267
Size: 4247
MluI A'CGCG_T
Cuts at: 3680 3680
Size: 4247
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2918 3102 2918
Size: 184 4063
MscI TGG'CCA
Cuts at: 577 820 973 2008 577
Size: 243 153 1035 2816
Fragments arranged by size:
2816 1035 243 153
MslI CAynn'nnrTG
Cuts at: 220 778 895 907 1207 2236 2363 220
Size: 558 117 12 300 1029 127 2104
Fragments arranged by size:
2104 1029 558 300 127 117 12
MspA1I CmG'CkG
Cuts at: 936 1061 1358 1672 2032 3045 3290 936
Size: 125 297 314 360 1013 245 1893
Fragments arranged by size:
1893 1013 360 314 297 245 125
NaeI GCC'GGC
Cuts at: 2428 2428
Size: 4247
NarI GG'CG_CC
Cuts at: 1678 1926 1678
Size: 248 3999
NcoI C'CATG_G
Cuts at: 107 107
Size: 4247
NgoMIV G'CCGG_C
Cuts at: 2426 2426
Size: 4247
NspI r_CATG'y
Cuts at: 540 757 1300 1800 2707 540
Size: 217 543 500 907 2080
Fragments arranged by size:
2080 907 543 500 217
PciI A'CATG_T
Cuts at: 2703 2703
Size: 4247
PflMI CCAn_nnn'nTGG
Cuts at: 470 969 470
Size: 499 3748
PmeI GTTT'AAAC
Cuts at: 1246 1246
Size: 4247
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3386 3418 3386
Size: 32 4215
PpuMI rG'GwC_Cy
Cuts at: 1134 1134
Size: 4247
PstI C_TGCA'G
Cuts at: 1294 1979 1294
Size: 685 3562
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4247
PvuII CAG'CTG
Cuts at: 936 1672 2032 936
Size: 736 360 3151
Fragments arranged by size:
3151 736 360
RsrII CG'GwC_CG
Cuts at: 2442 2442
Size: 4247
SacI G_AGCT'C
Cuts at: 689 1007 1261 689
Size: 318 254 3675
Fragments arranged by size:
3675 318 254
SalI G'TCGA_C
Cuts at: 1284 1284
Size: 4247
SapI GCTCTTCn'nnn_
Cuts at: 1132 2270 2480 1132
Size: 1138 210 2899
Fragments arranged by size:
2899 1138 210
SbfI CC_TGCA'GG
Cuts at: 1294 1294
Size: 4247
ScaI AGT'ACT
Cuts at: 3764 3764
Size: 4247
SfcI C'TryA_G
Cuts at: 33 453 1041 1290 1975 2968 3159 33
Size: 420 588 249 685 993 191 1121
Fragments arranged by size:
1121 993 685 588 420 249 191
SfoI GGC'GCC
Cuts at: 1679 1927 1679
Size: 248 3999
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4247
SmaI CCC'GGG
Cuts at: 1269 1269
Size: 4247
SmlI C'TyrA_G
Cuts at: 373 1402 2317 2809 3071 373
Size: 1029 915 492 262 1549
Fragments arranged by size:
1549 1029 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4247
SphI G_CATG'C
Cuts at: 540 1300 1800 540
Size: 760 500 2987
Fragments arranged by size:
2987 760 500
StuI AGG'CCT
Cuts at: 763 763
Size: 4247
StyI C'CwwG_G
Cuts at: 107 1381 1735 107
Size: 1274 354 2619
Fragments arranged by size:
2619 1274 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 400 1830 1963 2422 2631 400
Size: 1430 133 459 209 2016
Fragments arranged by size:
2016 1430 459 209 133
TatI w'GTAC_w
Cuts at: 299 692 3762 299
Size: 393 3070 784
Fragments arranged by size:
3070 784 393
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 702 734 3764 3796 702
Size: 32 3030 32 1153
Fragments arranged by size:
3030 1153 32 32
Tth111I GACn'n_nGTC
Cuts at: 2044 2044
Size: 4247
XbaI T'CTAG_A
Cuts at: 1278 1278
Size: 4247
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1742 1742
Size: 4247
XmaI C'CCGG_G
Cuts at: 1267 1267
Size: 4247
ZraI GAC'GTC
Cuts at: 1035 1035
Size: 4247
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflIII AgeI AleI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BbvCI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssSI BstAPI BstBI BstXI BstYI BtgI
BtgZI BtsI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NspI PciI PflMI
PmeI PpiI PpuMI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI ZraI
Enzymes that do not cut:
AarI AfeI AflII AhdI AlfI AloI ApaI AscI
AvrII BaeI BcgI BfrBI BmtI BsgI BsiWI BsmI
BsmBI BssHII BstEII BstZ17I Bsu36I ClaI CspCI DraIII
EcoRV FseI FspAI HindIII HpaI MfeI NdeI NheI
NotI NruI NsiI PacI PasI PfoI PmlI PshAI
PsiI PspOMI PspXI PsrI SacII SanDI SexAI SfiI
SgrAI SnaBI SrfI SspI SwaI XhoI XmnI