Restriction map for pF1KB5273
 (Circular) (Six-base) MAPSORT of: pF1KB5273.seq  Check: 8513  from: 1  to: 3509



pF1KB5273  3509 bp

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:15  ..

AccI GT'mk_AC

Cuts at:    547     547 
   Size:       3509
 
Acc65I G'GTAC_C

Cuts at:    525     525 
   Size:       3509
 
AclI AA'CG_TT

Cuts at:   3349    3349 
   Size:       3509
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1334    1766    2513    2781    1334 
   Size:        432     747     268    2062

  Fragments arranged by size: 

                2062     747     432     268
 
AflIII A'CryG_T

Cuts at:    861    1965    2942     861 
   Size:       1104     977    1428

  Fragments arranged by size: 

                1428    1104     977
 
AgeI A'CCGG_T

Cuts at:   1897    1897 
   Size:       3509
 
AlwNI CAG_nnn'CTG

Cuts at:    814    2381     814 
   Size:       1567    1942
 
ApaLI G'TGCA_C

Cuts at:    480    2279     480 
   Size:       1799    1710
 
ApoI r'AATT_y

Cuts at:    118     210     316     513    2862    3118     118 
   Size:         92     106     197    2349     256     509

  Fragments arranged by size: 

                2349     509     256     197     106      92
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       3509
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       3509
 
AvaI C'yCGr_G

Cuts at:    529     529 
   Size:       3509
 
BamHI G'GATC_C

Cuts at:    534     534 
   Size:       3509
 
BanI G'GyrC_C

Cuts at:    127     525     939    1187    1222     127 
   Size:        398     414     248      35    2414

  Fragments arranged by size: 

                2414     414     398     248      35
 
BanII G_rGCy'C

Cuts at:    523    1553     523 
   Size:       1030    2479
 
BbeI G_GCGC'C

Cuts at:    943    1191     943 
   Size:        248    3261
 
BciVI GTATCCnnnnn_n'

Cuts at:   1407    2168    3509    1407 
   Size:        761    1341    1407

  Fragments arranged by size: 

                1407    1341     761
 
BclI T'GATC_A

Cuts at:    230    1029     230 
   Size:        799    2710
 
BglII A'GATC_T

Cuts at:   1024    3061    1024 
   Size:       2037    1472
 
BlpI GC'TnA_GC

Cuts at:    621     621 
   Size:       3509
 
Bme1580I G_kGCm'C

Cuts at:    130     484    1134    1227    2283     130 
   Size:        354     650      93    1056    1356

  Fragments arranged by size: 

                1356    1056     650     354      93
 
BmrI ACTGGGnnnn_n'

Cuts at:    214     721     802    1137    3281     214 
   Size:        507      81     335    2144     442

  Fragments arranged by size: 

                2144     507     442     335      81
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   1876    1876 
   Size:       3509
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:    685    1564    2056    2354     685 
   Size:        879     492     298    1840

  Fragments arranged by size: 

                1840     879     492     298
 
BsaI GGTCTCn'nnnn_

Cuts at:   3213    3213 
   Size:       3509
 
BsaAI yAC'GTr

Cuts at:    862     862 
   Size:       3509
 
BsaBI GATnn'nnATC

Cuts at:      6    1023    3060       6 
   Size:       1017    2037     455

  Fragments arranged by size: 

                2037    1017     455
 
BsaHI Gr'CG_yC

Cuts at:    940    1188    1890    3386     940 
   Size:        248     702    1496    1063

  Fragments arranged by size: 

                1496    1063     702     248
 
BsaWI w'CCGG_w

Cuts at:    698    1219    1897    2171    2318    2729     698 
   Size:        521     678     274     147     411    1478

  Fragments arranged by size: 

                1478     678     521     411     274     147
 
BseYI C'CCAG_C

Cuts at:    934    1692    2269    2765     934 
   Size:        758     577     496    1678

  Fragments arranged by size: 

                1678     758     577     496
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:    412     412 
   Size:       3509
 
BsiEI CG_ry'CG

Cuts at:    104    1051    1097    2305     104 
   Size:        947      46    1208    1308

  Fragments arranged by size: 

                1308    1208     947      46
 
BsiHKAI G_wGCw'C

Cuts at:    484     523    1301    1491    2283     484 
   Size:         39     778     190     792    1710

  Fragments arranged by size: 

                1710     792     778     190      39
 
Bsp1286I G_dGCh'C

Cuts at:    130     484     523    1134    1227    1301    1491    1553    2283 
   Size:        354      39     611      93      74     190      62     730

Cuts at:   2283     130 
   Size:       1356

  Fragments arranged by size: 

                1356     730     611     354     190      93      74      62
                  39
 
BspEI T'CCGG_A

Cuts at:   2729    2729 
   Size:       3509
 
BspHI T'CATG_A

Cuts at:   2685    2685 
   Size:       3509
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    559     773    1075    1456     559 
   Size:        214     302     381    2612

  Fragments arranged by size: 

                2612     381     302     214
 
BsrBI CCG'CTC

Cuts at:     50    1801    1855    3339    3504      50 
   Size:       1751      54    1484     165      55

  Fragments arranged by size: 

                1751    1484     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:   1421    1421 
   Size:       3509
 
BsrFI r'CCGG_y

Cuts at:   1507    1688    1897    1507 
   Size:        181     209    3119

  Fragments arranged by size: 

                3119     209     181
 
BssSI C'ACGA_G

Cuts at:   1780    2138    1780 
   Size:        358    3151
 
BstAPI GCAn_nnn'nTGC

Cuts at:    886     886 
   Size:       3509
 
BstBI TT'CG_AA

Cuts at:   1870    1870 
   Size:       3509
 
BstXI CCAn_nnnn'nTGG

Cuts at:    204     204 
   Size:       3509
 
BstYI r'GATC_y

Cuts at:      1     534    1001    1024    1359    1605    2617    2703    2715 
   Size:        533     467      23     335     246    1012      86      12

Cuts at:   2715    3061       1 
   Size:        346     449

  Fragments arranged by size: 

                1012     533     467     449     346     335     246      86
                  23      12
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:   1459    1640    1803    1459 
   Size:        181     163    3165

  Fragments arranged by size: 

                3165     181     163
 
BtsI GCAGTG_nn'

Cuts at:    752     752 
   Size:       3509
 
DraI TTT'AAA

Cuts at:    282     508     282 
   Size:        226    3283
 
DrdI GACnn_nn'nnGTC

Cuts at:   1215    2073    1215 
   Size:        858    2651
 
EaeI y'GGCC_r

Cuts at:    215    1094    1268    1659    1686    3031     215 
   Size:        879     174     391      27    1345     693

  Fragments arranged by size: 

                1345     879     693     391     174      27
 
EagI C'GGCC_G

Cuts at:   1094    1094 
   Size:       3509
 
EarI CTCTTCn'nnn_

Cuts at:   1532    1742    1532 
   Size:        210    3299
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:   1717    2027    2173    3319    1717 
   Size:        310     146    1146    1907

  Fragments arranged by size: 

                1907    1146     310     146
 
EcoICRI GAG'CTC

Cuts at:    521     521 
   Size:       3509
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:   1334    1766    2513    2781    1334 
   Size:        432     747     268    2062

  Fragments arranged by size: 

                2062     747     432     268
 
EcoO109I rG'GnC_Cy

Cuts at:    648     648 
   Size:       3509
 
EcoRI G'AATT_C

Cuts at:    513     513 
   Size:       3509
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   1528    1528 
   Size:       3509
 
FspI TGC'GCA

Cuts at:   1290    1290 
   Size:       3509
 
HaeII r_GCGC'y

Cuts at:    943    1191    2213    3192     943 
   Size:        248    1022     979    1260

  Fragments arranged by size: 

                1260    1022     979     248
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1351    1381    1383    1413    1351 
   Size:         30       2      30    3447

  Fragments arranged by size: 

                3447      30      30       2
 
HincII GTy'rAC

Cuts at:    548     548 
   Size:       3509
 
KasI G'GCGC_C

Cuts at:    939    1187     939 
   Size:        248    3261
 
KpnI G_GTAC'C

Cuts at:    529     529 
   Size:       3509
 
MluI A'CGCG_T

Cuts at:   2942    2942 
   Size:       3509
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   2180    2364    2180 
   Size:        184    3325
 
MscI TGG'CCA

Cuts at:    217    1270     217 
   Size:       1053    2456
 
MslI CAynn'nnrTG

Cuts at:   1498    1625    1498 
   Size:        127    3382
 
MspA1I CmG'CkG

Cuts at:    334     620     934    1294    2307    2552     334 
   Size:        286     314     360    1013     245    1291

  Fragments arranged by size: 

                1291    1013     360     314     286     245
 
NaeI GCC'GGC

Cuts at:   1690    1690 
   Size:       3509
 
NarI GG'CG_CC

Cuts at:    940    1188     940 
   Size:        248    3261
 
NgoMIV G'CCGG_C

Cuts at:   1688    1688 
   Size:       3509
 
NspI r_CATG'y

Cuts at:    562    1062    1969     562 
   Size:        500     907    2102

  Fragments arranged by size: 

                2102     907     500
 
PciI A'CATG_T

Cuts at:   1965    1965 
   Size:       3509
 
PmeI GTTT'AAAC

Cuts at:    508     508 
   Size:       3509
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2648    2680    2648 
   Size:         32    3477
 
PsiI TTA'TAA

Cuts at:    256     256 
   Size:       3509
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:    258     290     258 
   Size:         32    3477
 
PstI C_TGCA'G

Cuts at:    371     556    1241     371 
   Size:        185     685    2639

  Fragments arranged by size: 

                2639     685     185
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       3509
 
PvuII CAG'CTG

Cuts at:    334     934    1294     334 
   Size:        600     360    2549

  Fragments arranged by size: 

                2549     600     360
 
RsrII CG'GwC_CG

Cuts at:   1704    1704 
   Size:       3509
 
SacI G_AGCT'C

Cuts at:    523     523 
   Size:       3509
 
SalI G'TCGA_C

Cuts at:    546     546 
   Size:       3509
 
SapI GCTCTTCn'nnn_

Cuts at:   1532    1742    1532 
   Size:        210    3299
 
SbfI CC_TGCA'GG

Cuts at:    556     556 
   Size:       3509
 
ScaI AGT'ACT

Cuts at:   3026    3026 
   Size:       3509
 
SfcI C'TryA_G

Cuts at:     33     367     552    1237    2230    2421      33 
   Size:        334     185     685     993     191    1121

  Fragments arranged by size: 

                1121     993     685     334     191     185
 
SfoI GGC'GCC

Cuts at:    941    1189     941 
   Size:        248    3261
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       3509
 
SmaI CCC'GGG

Cuts at:    531     531 
   Size:       3509
 
SmlI C'TyrA_G

Cuts at:    664    1579    2071    2333     664 
   Size:        915     492     262    1840

  Fragments arranged by size: 

                1840     915     492     262
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       3509
 
SphI G_CATG'C

Cuts at:    562    1062     562 
   Size:        500    3009
 
StyI C'CwwG_G

Cuts at:    643     997     643 
   Size:        354    3155
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    169    1092    1225    1684    1893     169 
   Size:        923     133     459     209    1785

  Fragments arranged by size: 

                1785     923     459     209     133
 
TatI w'GTAC_w

Cuts at:   3024    3024 
   Size:       3509
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   3026    3058    3026 
   Size:         32    3477
 
Tth111I GACn'n_nGTC

Cuts at:   1306    1306 
   Size:       3509
 
XbaI T'CTAG_A

Cuts at:    540     540 
   Size:       3509
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:    201    1004     201 
   Size:        803    2706
 
XmaI C'CCGG_G

Cuts at:    529     529 
   Size:       3509
 

 Enzymes that do cut:

     AccI   Acc65I     AclI     AcuI   AflIII     AgeI    AlwNI    ApaLI
     ApoI     AseI    AsiSI     AvaI    BamHI     BanI    BanII     BbeI
    BciVI     BclI    BglII     BlpI Bme1580I     BmrI     BplI    BpuEI
     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BseYI     BsgI    BsiEI
  BsiHKAI Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI
    BssSI   BstAPI    BstBI    BstXI    BstYI    BtgZI     BtsI     DraI
     DrdI     EaeI     EagI     EarI     EciI  EcoICRI  Eco57MI EcoO109I
    EcoRI     FalI     FspI    HaeII    Hin4I   HincII     KasI     KpnI
     MluI     MmeI     MscI     MslI   MspA1I     NaeI     NarI   NgoMIV
     NspI     PciI     PmeI     PpiI     PsiI     PsrI     PstI     PvuI
    PvuII    RsrII     SacI     SalI     SapI     SbfI     ScaI     SfcI
     SfoI     SgfI     SmaI     SmlI     SpeI     SphI     StyI    TaqII
     TatI     TstI  Tth111I     XbaI     XcmI     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AfeI    AflII     AhdI     AleI     AlfI     AloI
     ApaI     AscI    AvrII     BaeI     BbsI    BbvCI     BcgI    BfrBI
     BglI    BmgBI     BmtI     BpmI   Bpu10I    BsaXI    BseRI    BsiWI
     BsmI    BsmBI    BsrGI   BssHII   BstEII  BstZ17I   Bsu36I     BtgI
     ClaI    CspCI   DraIII    EcoNI    EcoRV     FseI    FspAI  HindIII
     HpaI     MfeI     NcoI     NdeI     NheI     NotI     NruI     NsiI
     PacI     PasI    PflMI     PfoI     PmlI    PpuMI    PshAI   PspOMI
    PspXI    SacII    SanDI    SexAI     SfiI    SgrAI    SnaBI     SrfI
     SspI     StuI     SwaI     XhoI     XmnI     ZraI
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